Abstract：
:Absorption rates for many biologically important compounds are determined by the relative hydrophobicity of the jejunal microvillus membrane. An estimate of this parameter may be obtained by measuring the incremental change in free energy that occurs when a methylene group partitions into the bilayer form an external aqueous solution. Although sensitive, this measurement has been difficult to quantitate in vivo; therefore, these studies have historically been performed in vitro. We describe a rapid, simple technique to measure this parameter in vivo. Furthermore, this method directly quantitates the resistance of aqueous unstirred layers that lie external to the microvillus membrane.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Meddings JB,Dietschy JMsubject
Has Abstractpub_date
1989-08-01 00:00:00pages
1289-96issue
8eissn
0022-2275issn
1539-7262journal_volume
30pub_type
杂志文章abstract：:Four distinct subspecies of the insect hemolymph lipoprotein, lipophorin, that range in diacylglycerol (DAG) content from approximately 100 to 1000 molecules per particle, were treated with phospholipase C. Lipid analysis demonstrated that both phosphatidylcholine and phosphatidylethanolamine were hydrolyzed to DAG. P...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:In mammals, because they share a single synthetic pathway, n-6/n-3 ratios of dietary PUFAs impact tissue arachidonic acid (ARA) and DHA content. Likewise, SNPs in the human fatty acid desaturase (FADS) gene cluster impact tissue ARA and DHA. Here we tested the feasibility of using heterozygous Fads2-null-mice (HET) as...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M064956
更新日期：2016-11-01 00:00:00
abstract：:It has been suggested that phytosterol and phytostanol esters possess similar cholesterol-lowering properties, however, whether mechanisms responsible are identical has not been addressed. To address this question, cholesterol plasma levels, absorption, biosynthesis, and turnover were measured in 15 hypercholesterolem...
journal_title：Journal of lipid research
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2000-05-01 00:00:00
abstract：:The effect of fatty acids on secretion of triglyceride-rich lipoprotein (d less than 1.006 g/ml) by CaCo-2 cells was studied. Of the fatty acids studied, oleic acid (18:1) was the most potent stimulator of newly synthesized triglyceride secretion in triglyceride-rich lipoproteins followed in descending order by 18:2, ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:Alzheimer's disease (AD) is characterized by an extensive accumulation of amyloid-β (Aβ) peptide, which triggers a set of deleterious processes, including synaptic dysfunction, inflammation, and neuronal injury, leading to neuronal loss and cognitive impairment. A large body of evidence supports that nuclear receptor ...
journal_title：Journal of lipid research
pub_type： 杂志文章,评审
doi：10.1194/jlr.R075556
更新日期：2017-10-01 00:00:00
abstract：:A method for the preparation of 1-alkyl 2-acyl glycero-3-phosphorylcholine, labeled in the 2-position with oleic acid-1-(14)C is described. This method, based on the acylation of a 1-alkyl glycero-3-phosphorylcholine-CdCl(2) complex which is, in turn, formed from 1-alk-1-enyl glycero-3-phosphorylcholine by hydrogenati...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1970-09-01 00:00:00
abstract：:The effect of 26-hydroxycholesterol and other intermediates in bile acid synthesis on HMG-CoA reductase activity was studied in Chinese hamster ovary (CHO) cell culture. Incubation of CHO cells for 5 hr in 0.25 microM 26-hydroxycholesterol caused a 40% inhibition of HMG-CoA reductase activity. All other intermediates ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:Human high density lipoprotein enriched in free cholesterol was obtained by exposing the lipoprotein to lipid dispersions having a free cholesterol/lecithin molar ratio greater than two. The metabolism of cholesterol was studied in tissue culture cells exposed to normal and cholesterol-enriched lipoproteins. Incubatio...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:In an effort to explore the feasibility of converting a lipase into an esterase by modifying the lid region, we designed and characterized two novel Rhizopus chinensis lipase variants by lid swapping. The substrate specificity of an R. chinensis lipase was successfully modified toward water-soluble substrates, that is...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M043950
更新日期：2014-06-01 00:00:00
abstract：:Some commercial preparations of common natural conjugated bile salts contain impurities (e.g., amines, lipids, and calcium) that are likely to affect their physicochemical properties. A method was developed for purifying commercial preparations of sodium salts of glycine- and taurine-conjugated bile acids. The method ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:Elevated serum levels of triglyceride-rich remnant lipoproteins (TRL) are a major risk factor predisposing a subject to atherosclerosis. Apolipoprotein C-III (apoC-III) is a major constituent of TRL that impedes triglyceride hydrolysis and remnant clearance and, as such, may exert pro-atherogenic activities. In the pr...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.m200386-jlr200
更新日期：2002-12-01 00:00:00
abstract：:The simultaneous exchange of (3h)tocopherol and (14C)cholesterol between rat plasma, rat plasma lipoproteins, and RBC was studied in vitro to compare quantitavely (a) the fractional exchange rates and (b) the half-times for isotope equilibration. In all incubations of RBC with plasma or with plasma lipoprotein fractio...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Familial dysbetalipoproteinemia (FD) is a genetic disorder associated with impaired postprandial lipid clearance. The effect of adding bezafibrate to standard lipid-lowering therapy on postprandial and fasting lipid levels in patients with FD is unknown. In this randomized placebo-controlled double-blind crossover tri...
journal_title：Journal of lipid research
pub_type： 杂志文章,随机对照试验
doi：10.1194/jlr.M076901
更新日期：2017-11-01 00:00:00
abstract：:We have studied the recognition and uptake of acid lipase by human fibroblasts in order to determine requirements for localization and function of the enzyme in lysosomes. Our approach was based on evidence that a number of acid hydrolases involved in mucopolysaccharide metabolism are secreted from cultured fibroblast...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:The diffusion of [2-(14)C]glucose out of liposomes prepared from extracted human erythrocyte lipids was examined. Increased glucose efflux was observed when the lipids were treated with hydrogen peroxide and CuCl(2) before liposome formation, and this phenomenon required both peroxide and metal. Peroxidation of these ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1974-05-01 00:00:00
abstract：:Sterol-regulatory element binding protein-1c (SREBP-1c) is a transcription factor that controls lipogenesis in the liver. Hepatic SREBP-1c is nutritionally regulated, and its sustained activation causes hepatic steatosis and insulin resistance. Although regulation of SREBP-1c is known to occur at the transcriptional l...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M004234
更新日期：2010-07-01 00:00:00
abstract：:A colony of New Zealand white rabbits has been developed which, when fed a cholesterol-supplemented diet, exhibit unusual resistance to hypercholesterolemia and atherosclerosis, disorders usually observed in normal cholesterol-fed rabbits. When resistant rabbits (RT) were fed a normal low cholesterol diet (ND), their ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:Recent evidence suggests the possibility that macrophages can influence lipoprotein metabolism. Therefore we investigated the ability of cultured macrophages to alter low density lipoprotein (LDL) uptake in a human liver cell line (HepG2). Conditioned media from phlogogenic-induced mouse peritoneal macrophages or from...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Achilles tendon xanthomas are associated with increased cardiovascular risk in patients with familial hypercholesterolemia (FH). Oxidized low density lipoprotein (OxLDL), the antibodies against OxLDL, and the LDL-associated phospholipase A(2) (Lp-PLA(2)) may play important roles in atherogenesis. We investigated the p...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M600109-JLR200
更新日期：2006-10-01 00:00:00
abstract：:The extent to which cholesterol synthesis is modulated in macrophage foam cells by changes in cholesterol influx and efflux was determined using thioglycollate-elicited peritoneal macrophages from normal and cholesterol-fed White Carneau (WC) and Show Racer (SR) pigeons. In peritoneal macrophages from normocholesterol...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:Sequence homology indicates the existence of three human cytosolic acyl protein thioesterases, including APT1 that is known to depalmitoylate H- and N-Ras. One of them is the lysophospholipase-like 1 (LYPLAL1) protein that on the one hand is predicted to be closely related to APT1 but on the other hand might also func...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M019851
更新日期：2012-01-01 00:00:00
abstract：:Severe endothelial abnormalities are a prominent feature in sepsis with cytokines such as tumor necrosis factor (TNF)alpha being implicated in the pathogenesis. As mimic to inflammation, human umbilical vascular endothelial cells (HUVEC) were incubated with TNFalpha for 22 h, in the absence or presence of the omega-6 ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：:Bis(monoacylglycero)phosphate (BMP), also known as lysobisphosphatidic acid, is a phospholipid that promotes lipid sorting in late endosomes/lysosomes by activating lipid hydrolases and lipid transfer proteins. Changes in the cellular BMP content therefore reflect an altered metabolic activity of the endolysosomal sys...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.RA119000516
更新日期：2020-07-01 00:00:00
abstract：:Diseases such as obesity, diabetes, and atherosclerosis result from multiple genetic and environmental factors, and importantly, interactions between genetic and environmental factors. Identifying susceptibility genes for these diseases using genetic and genomic technologies is accelerating, and the expectation over t...
journal_title：Journal of lipid research
pub_type： 杂志文章,评审
doi：10.1194/jlr.R600026-JLR200
更新日期：2006-12-01 00:00:00
abstract：:Apoliprotein (apo) B-100-containing very low density lipoprotein (VLDL) particles secreted from the liver accumulate in plasma during alimentary lipemia. To determine whether changes of VLDL composition occur in the postprandial state that may render these lipoproteins more atherogenic, apoE, C-I, C-II, and C-III, and...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:Three glycosides of 13-hydroxydocosanoic acid isolated from Candida bogoriensis were characterized by quantitating the amount of carbohydrate, acetate, and hydroxy acid in each, and by gas-liquid chromatography and mass spectrometry of their methyl ester, trimethylsilyl ether derivatives. One of the glycosides was the...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1972-09-01 00:00:00
abstract：:Each abdominal fat depot, such as mesenteric or epididymal, differently contributes to the development of insulin resistance. The aim of this study was to identify the genetic regions that contribute to fat accumulation in epididymal/mesenteric fat and to examine whether or not the genetic regions that affect glucose ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M009563
更新日期：2010-12-01 00:00:00
abstract：:apoE is a multi-functional protein expressed in several cell types and in several organs. It is highly expressed in adipose tissue, where it is important for modulating adipocyte lipid flux and gene expression in isolated adipocytes. In order to investigate a potential systemic role for apoE that is produced in adipos...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M050567
更新日期：2015-02-01 00:00:00
abstract：:Significant amounts of ursodeoxycholic acid (UDCA) used for the treatment of patients with primary biliary cirrhosis (PBC) become epimerized at C-3 to isoUDCA. We investigated the metabolism of isoUDCA and a possible pharmacologic effect in five patients (51.4 +/- 5.8 years old; 3 females, 2 males) with PBC and persis...
journal_title：Journal of lipid research
pub_type： 临床试验,杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:Using [13C]-tracers and direct fetal doses, we show for the first time that the fetal primate converts alpha-linolenic acid (18:3) to docosahexaenoic acid (22:6) in vivo, and we estimate the relative bioefficacy of the two substrates for brain 22:6 accretion. Pregnant female baboons consumed a diet free of long chain ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
