Abstract：
:The opportunistic human pathogen Penicillium marneffei exhibits a temperature-dependent dimorphic switch. At 25 degrees, multinucleate, septate hyphae that can undergo differentiation to produce asexual spores (conidia) are produced. At 37 degrees hyphae undergo arthroconidiation to produce uninucleate yeast cells that divide by fission. This work describes the cloning of the P. marneffei gasC gene encoding a G-protein alpha-subunit that shows high homology to members of the class III fungal Galpha-subunits. Characterization of a DeltagasC mutant and strains carrying a dominant-activating gasC(G45R) or a dominant-interfering gasC(G207R) allele show that GasC is a crucial regulator of germination. A DeltagasC mutant is severely delayed in germination, whereas strains carrying a dominant-activating gasC(G45R) allele show a significantly accelerated germination rate. Additionally, GasC signaling positively affects the production of the red pigment by P. marneffei at 25 degrees and negatively affects the onset of conidiation and the conidial yield, showing that GasC function overlaps with functions of the previously described Galpha-subunit GasA. In contrast to the S. cerevisiae ortholog Gpa2, our data indicate that GasC is not involved in carbon or nitrogen source sensing and plays no major role in either hyphal or yeast growth or in the switch between these two forms.
journal_name
Geneticsjournal_title
Geneticsauthors
Zuber S,Hynes MJ,Andrianopoulos Akeywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
487-99issue
2eissn
0016-6731issn
1943-2631journal_volume
164pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The Arp2/3 complex is an essential component of the actin cytoskeleton in yeast and is required for the movement of actin patches. In an attempt to identify proteins that interact with this complex in the fission yeast Schizosaccharomyces pombe, we sought high-copy suppressors of the S. pombe arp3-c1 mutant, and have ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:A coalescent theory for a sample of DNA sequences from a partially selfing diploid population and an algorithm for simulating such samples are developed in this article. Approximate formulas are given for the expectation and the variance of the number of segregating sites in a sample of k sequences from n individuals....
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:I reexamine the use of isolation by distance models as a basis for the estimation of demographic parameters from measures of population subdivision. To that aim, I first provide results for values of F-statistics in one-dimensional models and coalescence times in two-dimensional models, and make more precise earlier r...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:Dna2 is a nuclease and helicase that functions redundantly with other proteins in Okazaki fragment processing, double-strand break resection, and checkpoint kinase activation. Dna2 is an essential enzyme, required for yeast and mammalian cell viability. Here, we report that numerous mutations affecting the DNA damage ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300809
更新日期：2018-05-01 00:00:00
abstract：:In the yeast Saccharomyces cerevisiae ectopic recombination has been shown to occur at high frequencies for artificially created repeats, but at relatively low frequencies for a natural family of repeated sequences, the Ty family. Little is known about the mechanism(s) that prevent recombination between repeated seque...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:We derive a formula for the distribution of the length T of the recombination interval containing a target gene and using N gametes in a region where R kilobases correspond to 1 cM. The formula can be used to calculate the number of meiotic events required to narrow a target gene down to a specific interval size and h...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:A canon of population genetics concerns the properties of FST, a descriptor of spatial genetic structure. Interest for FST arose from Wright's early insights linking FST to dispersal parameters as well as to his concept of effective population size (e.g., Wright 1938, 1951). Although there is continued interest in thi...
journal_title：Genetics
pub_type： 评论,杂志文章
doi：10.1534/genetics.113.152132
更新日期：2013-07-01 00:00:00
abstract：:The mak7-1 mutant loses the killer toxin-encoding M1 dsRNA. MAK7 is RPL4A, one of two genes encoding ribosomal protein L4. KRB1 is a dominant suppressor of mak7-1 that is tightly centromerelinked, but not linked to centromere markers of chromosomes I-XVI. Our orthogonal field agarose gel electrophoresis analysis of ch...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:The application of quantitative genetics in plant and animal breeding has largely focused on additive models, which may also capture dominance and epistatic effects. Partitioning genetic variance into its additive and nonadditive components using pedigree-based models (P-genomic best linear unbiased predictor) (P-BLUP...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.171322
更新日期：2014-12-01 00:00:00
abstract：:We describe here a new variant of transposon Tn10 especially adapted for transposon analysis of cloned yeast genes; it can equally well be used for analysis of prokaryotic genes. We have applied this element to analysis of the LEU2, RAD50, and CDC48 genes of Saccharomyces cerevisiae. This transposon, nicknamed mini-Tn...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:Microsatellites have been widely used as tools for population studies. However, inference about population processes relies on the specification of mutation parameters that are largely unknown and likely to differ across loci. Here, we use data on somatic mutations to investigate the mutation process at 14 tetranucleo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:Hormonal, genetic, and environmental factors play major roles in the complex etiology of breast cancer. When treated continuously with 17beta-estradiol (E2), the ACI rat exhibits a genetically conferred propensity to develop mammary cancer. The susceptibility of the ACI rat to E2-induced mammary cancer appears to segr...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.033878
更新日期：2004-12-01 00:00:00
abstract：:As the dynamic nature of progenitor genomes accompanies the speciation by interspecific hybridization, the extraction of the constituent subgenome(s) from a natural allopolyploid species of long history and then restitution of the progenitor(s) provides the unique opportunity to study the genome evolution and interpla...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.190967
更新日期：2016-11-01 00:00:00
abstract：:The organization of the mitochondrial genome in plants is not well understood. In maize mitochondrial DNA (mtDNA) several subgenomic circular molecules as well as an abundant fraction of linear molecules have been seen by electron microscopy. It has been hypothesized that the circular molecules are the genetic entitie...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:We demonstrate that the previously described gene Androgen binding protein (Abp; Dlouhy and Karn, 1984) codes for the Alpha subunit of ABP and rename the locus Androgen binding protein alpha (Abpa). A study of recombinant inbred strains demonstrates that Abpa is located on chromosome 7 near Glucose phosphate isomerase...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:The RNA polymerase II (Pol-II) holoenzyme, responsible for messenger RNA production, typically consists of 10-12 subunits. Our laboratory previously demonstrated that maternally deposited, long, noncoding, template RNAs are essential for programmed genome rearrangements in the ciliate Oxytricha trifallax. Here we show...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.163279
更新日期：2014-07-01 00:00:00
abstract：:Two composite multiple regression-interval mapping analyses were performed to identify candidate quantitative trait loci (QTL) affecting components of wing shape in Drosophila melanogaster defined by eight relative warp-based measures. A recombinant inbred line design was used to map QTL for the shape of two intervein...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:The mitochondrial DNA (mtDNA) in animals is generally a circular molecule of approximately 15 kb, but there are many exceptions such as linear molecules and larger ones. RFLP studies indicated that the mtDNA in the terrestrial isopod Armadillidium vulgare varied from 20 to 42 kb. This variation depended on the restric...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The idealized individual in many fungal species is a haploid self-sterile hermaphrodite that may be propagated by asexually produced spores or that may reproduce sexually. In field populations, polymorphism occurs for female-sterile/hermaphrodite status, and female-sterile mutants, which function only as males during ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:As part of our investigation of disease resistance in lettuce, we generated mutants that have lost resistance to Bremia lactucae, the casual fungus of downy mildew. Using a rapid and reliable screen, we identified 16 distinct mutants of Latuca sativa that have lost activity of one of four different downy mildew resist...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:Self-incompatibility (SI) is a genetic system found in some hermaphrodite plants. Recognition of pollen by pistils expressing cognate specificities at two linked genes leads to rejection of self pollen and pollen from close relatives, i.e., to avoidance of self-fertilization and inbred matings, and thus increased outc...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.127399
更新日期：2011-07-01 00:00:00
abstract：:A genetic linkage map was constructed in a backcross family of the red flour beetle, Tribolium castaneum, based largely on sequences from bacterial artificial chromosome (BAC) ends and untranslated regions from random cDNA's. In most cases, dimorphisms were detected using heteroduplex or single-strand conformational p...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.032227
更新日期：2005-06-01 00:00:00
abstract：:In S. commune the frequency of recombination between the two subunits, alpha and beta, of the B incompatibility factor is genetically controlled. Analysis of the progeny obtained from crosses between high- and low-recombining strains indicates that the gene controlling recombination frequency in the B factor is linked...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-05-01 00:00:00
abstract：:The Yanomama Indians are a South American tribe distributed over an irregular area approximately 200 X 300 miles. The gene frequencies observed at 12 loci in 47 villages within this area have been analyzed for the occurrence of clines. Apparently significant clines are observed for alleles of the Rh, MNSs, Kidd, Gm, I...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:Schizosaccharomyces pombe cells that contain a mutation within rad9 are sensitive to ionizing radiation, UV light and hydroxyurea, relative to wild-type strains. In addition, the mutants are moderately hypomutable by UV and unable to delay initiation of mitosis after treatment with radiation or hydroxyurea. Three radi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:We identified the Drosophila melanogaster Signal peptide peptidase gene (Spp) that encodes a multipass transmembrane aspartyl protease. Drosophila SPP is homologous to the human signal peptide peptidase (SPP) and is distantly related to the presenilins. We show that, like human SPP, Drosophila SPP can proteolyze a mod...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.039933
更新日期：2005-05-01 00:00:00
abstract：:The timing of lethality caused by homozygosity for a null allele of the epidermal growth factor receptor (Egfrtm1Mag) in mice is strongly dependent on genetic background. Initial attempts to genetically map background modifiers using Swiss-derived, outbred CD-1 mice were unsuccessful. To investigate the genetic archit...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.103.020495
更新日期：2004-08-01 00:00:00
abstract：:BTB-zinc finger transcription factors play many important roles in metazoan development. In these proteins, the BTB domain is critical for dimerization and for recruiting cofactors to target genes. Identification of these cofactors is important for understanding how BTB-zinc finger proteins influence transcription. He...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.111591
更新日期：2010-04-01 00:00:00
abstract：:Animal microRNAs (miRNA) are implicated in the control of nearly all cellular functions. Due to high sequence redundancy within the miRNA gene pool, loss of most of these 21- to 24-bp long RNAs individually does not cause a phenotype. Thus, only very few miRNAs have been associated with clear functional roles. We cons...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.145383
更新日期：2012-12-01 00:00:00
abstract：:Significant correlations between allelic frequencies and environmental variables in a number of insect species have been demonstrated by multivariate techniques. Since many environmental variables show a strong relationship to geographic location and since gene flow between populations can also produce patterns of gen...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-05-01 00:00:00
