Abstract:
:Trans-10,cis-12 conjugated linoleic acid (CLA) has previously been shown to be the CLA isomer responsible for CLA-induced reductions in body fat in animal models, and we have shown that this isomer, but not the cis-9,trans-11 CLA isomer, specifically decreased triglyceride (TG) accumulation in primary human adipocytes in vitro. Here we investigated the mechanism behind the isomer-specific, CLA-mediated reduction in TG accumulation in differentiating human preadipocytes. Trans-10,cis-12 CLA decreased insulin-stimulated glucose uptake and oxidation, and reduced insulin-dependent glucose transporter 4 gene expression. Furthermore, trans-10,cis-12 CLA reduced oleic acid uptake and oxidation when compared with all other treatments. In parallel to CLA's effects on metabolism, trans-10,cis-12 CLA decreased, whereas cis-9,trans-11 CLA increased, the expression of peroxisome proliferator-activated receptor gamma (PPARgamma) and several of its downstream target genes when compared with vehicle controls. Transient transfections demonstrated that both CLA isomers antagonized ligand-dependent activation of PPARgamma. Collectively, trans-10,cis-12, but not cis-9, trans-11, CLA decreased glucose and lipid uptake and oxidation and preadipocyte differentiation by altering preadipocyte gene transcription in a manner that appeared to be due, in part, to decreased PPARgamma expression.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Brown JM,Boysen MS,Jensen SS,Morrison RF,Storkson J,Lea-Currie R,Pariza M,Mandrup S,McIntosh MKdoi
10.1194/jlr.M300001-JLR200keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
1287-300issue
7eissn
0022-2275issn
1539-7262pii
M300001-JLR200journal_volume
44pub_type
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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更新日期:1975-05-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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journal_title:Journal of lipid research
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更新日期:1998-05-01 00:00:00
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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