Abstract:
:Sequence tagged sites generated for 60 NotI clones (NotI-STSs) from human chromosome 3-specific NotI-jumping and NotI-linking libraries were physically located using PCR screening of a radiation hybrid (RH) GeneBridge4 panel. The NotI map of chromosome 3 was generated using these RH-mapping data and those obtained earlier by FISH and sequencing of the corresponding NotI clones. The sequences of the NotI clones showed significant homologies with known genes and/or ESTs for 58 NotI-STSs (97%). These 58 NotI clones displayed 91-100% identity to 54 genes and 23 cDNA/EST clones. One known and two hypothetical protein-coding genes were localized for the first time and nine cDNA clones (unknown genes) were also carefully mapped only in this work. Three newly mapped genes are histone gene H1X (NR1-BK20C) and genes for hypothetical proteins THC1032178 and THC1024604 (NL1-243).
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Sulimova GE,Kutsenko AS,Rakhmanaliev ER,Udina IG,Kompaniytsev AA,Protopopov AI,Moisjak EV,Klimov EA,Muravenko OV,Zelenin AV,Braga EA,Kashuba VI,Zabarovsky ER,Kisselev LLdoi
10.1159/000069814keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
177-83issue
2-3eissn
1424-8581issn
1424-859Xpii
69814journal_volume
98pub_type
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