Abstract:
:Enterohemorrhagic Escherichia coli O157 is one of the leading worldwide public health concerns, causing large outbreaks of hemorrhagic colitis as well as numerous small outbreaks and sporadic cases. The variability of restriction enzyme-digestion patterns of O157 genomes, which is widely used to distinguish strains in the molecular epidemiology of O157 infections, suggests the presence of some genomic diversity among the strains. Based on the complete genome sequence of O157 Sakai, we analyzed the whole genome structures of eight O157 strains displaying diverse XbaI-digestion patterns by a systematic PCR analysis that we have named whole genome PCR scanning. This analysis identified not only the O157-specific sequences that are highly conserved among the strains, but also revealed an unexpectedly high degree of genomic diversity. In particular, prophages, including Shiga toxin-transducing phages, exhibited extensive structural and positional diversity, implying that variation of bacteriophages is a major factor in generating genomic diversity among the O157 lineage.
journal_name
Proc Natl Acad Sci U S Aauthors
Ohnishi M,Terajima J,Kurokawa K,Nakayama K,Murata T,Tamura K,Ogura Y,Watanabe H,Hayashi Tdoi
10.1073/pnas.262441699keywords:
subject
Has Abstractpub_date
2002-12-24 00:00:00pages
17043-8issue
26eissn
0027-8424issn
1091-6490pii
262441699journal_volume
99pub_type
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