Abstract:
:A proteinaceous inhibitor of alpha-amylase, Tendamistat, was evaluated as an immunogen to induce antibodies that mimic the enzyme activity and to investigate a new strategy to produce catalytic antibodies. Anti-Tendamistat polyclonal antibodies (pAbs) were shown to cross-react with acarbose, a strong carbohydrate inhibitor of alpha-amylases, and the alpha-amylase carbohydrate substrates, maltotetraose and maltoheptaose. Catalytic features of pAbs were characterized after denaturing natural serum alpha-amylase by treatment at pH 10 and in the presence of EDTA. A significant residual alpha-amylase activity was detected and associated with the IgG fraction, demonstrating that some antibodies behave as a functional mimic of natural amylase. Such antibodies, which behave as an "internal image" of the alpha-amylase, were used as immunogens to elicit anti-idiotype antibodies. It was demonstrated that the anti-idiotype antiserum contains a significant amount of antibodies that bind to porcine pancreatic amylase, which shows that they contain structural information from the original hapten, Tendamistat.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Gonçalves O,Dintinger T,Blanchard D,Tellier Cdoi
10.1016/s0022-1759(02)00238-7keywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
29-37issue
1-2eissn
0022-1759issn
1872-7905pii
S0022175902002387journal_volume
269pub_type
杂志文章abstract::We present a scheme for routine screening of patients' sera for monoclonal immunoglobulin by isoelectric focusing in agarose gels. Using this method combined with immunofixation, we have been able to detect monoclonal immunoglobulin in the sera of patients, and to identify the isotype of the immunoglobulin when conven...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(83)90393-9
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abstract::A generally applicable method was developed for the rapid and quantitative detection of both toxin- and virus neutralizing antibodies. The method was optimized for three different biological agents, i.e., Shigella toxin, influenza viruses (A/Beying, A/Taiwan and B/Yamagata) and Chikungunya virus. The in vitro micro-cy...
journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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abstract::A solid-phase enzyme immunoassay for the measurement of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis is reported. Sufficient amounts of acetylcholine receptor for the sensitive detection of anti-acetylcholine receptor antibody were directly fixed to Costar serocluster 96-well E...
journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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abstract::A single-step method for purification of mouse monoclonal antibodies directly from ascitic fluids using hydroxylapatite column chromatography is described. The procedure yields highly purified IgG or IgM antibodies. The purified immunoglobulin is essentially free of contaminating mouse albumin, transferrin, and other ...
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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abstract::This paper examines the parameters necessary for the efficient measurement of anti-Theiler's murine encephalomyelitis virus (TMEV) antibodies in an affinity-dependent manner using a variation of a solid-phase particle concentration fluorescence immunoassay (PCFIA). By allowing antibody to react with fluorochrome-label...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(89)90384-0
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journal_title:Journal of immunological methods
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doi:10.1016/0022-1759(96)00044-0
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journal_title:Journal of immunological methods
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doi:10.1016/j.jim.2008.01.014
更新日期:2008-04-20 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(91)90234-7
更新日期:1991-11-05 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(90)90308-i
更新日期:1990-06-12 00:00:00
abstract::Using in vitro established tumour cell lines attempts were made to assess the suitability of tetrazolium salt reduction (MTT) assay to replace the conventional radioactive base techniques for measuring cell proliferation and cell killing. The optimum conditions for MTT loading time, concentration of MTT and the time f...
journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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abstract::A procedure is described making it possible to obtain quantitative data with the immunohistochemical peroxidase anti--peroxidase (PAP) technique. After applying PAP on fixed fibroblast preparations, peroxidase enzymic activity is measured on solubilized immune-complexes. The results show this technique to be sensitive...
journal_title:Journal of immunological methods
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abstract::In an attempt to find a direct method for the determination of circulating immune complexes in pathological sera we studied the ability of Cibacron Blue 3GA covalently bound to Sepharose 4B to bind heat- or antigen-aggregated human IgG. Blue-Sepharose was found to bind significant amounts of aggregated IgG (about 1.5 ...
journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
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pub_type: 杂志文章
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