Abstract:
:LRP6 is a cell surface molecule that plays a critical role in the Wnt signaling pathway, and is implicated in numerous human diseases. Studies of cellular signaling mediated by LRP6 have relied on overexpression experiments, due to the lack of good monoclonal antibodies (mAbs) reactive with native LRP6 ectodomain. By using native recombinant LRP6 ectodomain fragment produced in mammalian expression system, we succeeded in developing a panel of anti-human LRP6 mAbs. Selected mAbs were capable of staining endogenous LRP6 on cell surface by using flow cytometry and immunofluorescence microscopy, and enriching detergent-solubilized LRP6 from cell lysate by immunoprecipitation.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Yasui N,Mihara E,Nampo M,Tamura-Kawakami K,Unno H,Matsumoto K,Takagi Jdoi
10.1016/j.jim.2009.11.016subject
Has Abstractpub_date
2010-01-31 00:00:00pages
153-60issue
1-2eissn
0022-1759issn
1872-7905pii
S0022-1759(09)00352-4journal_volume
352pub_type
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