alpha4-Fucosyltransferase is regulated during flower development: increases in activity are targeted to pollen maturation and pollen tube elongation.


:alpha4-Fucosylation represents a final step of protein N- glycosylation. alpha4-fucosylated N-glycans are thought to be involved in cell-to-cell communication and recognition in primates and plants. Nevertheless, in the plant life cycle, the function of alpha4-fucosylation remains largely unknown. To gain an insight into the role of alpha4-fucosylation during development, the study focused on tobacco flowers. It is shown that an increase in alpha(1,4)fucosyltransferase (Fuc-T) activity is only observed during anther development, whereas it remains at a constant but low level (around 20 pmol Fuc h(-1) mg(-1) protein) in the gynoecium and perianth. At least a 4-fold higher activity is detected in mature pollen grains. These data suggest that alpha(1,4)Fuc-T activity is regulated during anther development. Furthermore, alpha(1,4)Fuc-T activity could be required during pollen tube elongation where the activity level peaks at 350 pmol h(-1) mg(-1) protein. Based on enzyme profile and cycloheximide effects on pollen germination and activity, it is hypothesized that the gene encoding alpha4-Fuc-T could be regulated late during pollen development. A potential role of alpha4- fucosylation during pollen tube elongation is also discussed.


J Exp Bot


Joly C,Léonard R,Maftah A,Riou-Khamlichi C





Has Abstract


2002-06-01 00:00:00












  • Combining QTL and co-expression analysis allowed identification of new candidates for oil accumulation in rapeseed.

    abstract::Quantitative trait loci (QTL) have been discovered in crops, where some of causal quantitative trait genes (QTGs) may not be functionally characterized even in model plant Arabidopsis. We propose an approach to delineate QTGs by coordinating expression of genes located within QTL in crops and known orthologs related w...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Cui Y,Zeng X,Xiong Q,Wei D,Liao J,Xu Y,Chen G,Zhou Y,Dong H,Wan H,Liu Z,Li J,Guo L,Jung C,He Y,Qian W

    更新日期:2020-11-29 00:00:00

  • Viscoelastic properties of cell walls of single living plant cells determined by dynamic nanoindentation.

    abstract::Plant development results from controlled cell divisions, structural modifications, and reorganizations of the cell wall. Thereby, regulation of cell wall behaviour takes place at multiple length scales involving compositional and architectural aspects in addition to various developmental and/or environmental factors....

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Hayot CM,Forouzesh E,Goel A,Avramova Z,Turner JA

    更新日期:2012-04-01 00:00:00

  • Light signals, phytochromes and cross-talk with other environmental cues.

    abstract::Plants have evolved highly complex sensory mechanisms to monitor their surroundings and adapt their growth and development to the prevailing environmental conditions. The integration of information from multiple environmental cues enables the co-ordination of development with favourable seasonal conditions and, ultima...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Franklin KA,Whitelam GC

    更新日期:2004-01-01 00:00:00

  • Differential expression of two barley XET-related genes during coleoptile growth.

    abstract::Plant cell elongation depends on the physical properties of the primary cell wall. Because xyloglucan endotransglycosylases (XETs) are enzymes that mediate cleavage and rejoining of the beta(1-4)-XG backbone of primary cell wall, they are potentially involved in cell elongation. In this paper, the growth of the barley...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Burstin J

    更新日期:2000-05-01 00:00:00

  • Variation of floret fertility in hexaploid wheat revealed by tiller removal.

    abstract::Grain number per spike, which is greatly influenced by floret fertility, is an important trait of wheat (Triticum aestivum L.) yield. Maximum floret primordia, fertile floret, and final grain number per spikelet are three crucial factors of floret fertility. Floral degradation plays a critical role in determining thes...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Guo Z,Schnurbusch T

    更新日期:2015-09-01 00:00:00

  • The Arabidopsis AtEm1 promoter is active in Brassica napus L. and is temporally and spatially regulated.

    abstract::The promoter of the Arabidopsis thaliana L. AtEm1 gene encoding a late embryogenesis abundant protein was fused to the beta-glucuronidase reporter gene and introduced into Brassica napus. The promoter is highly active in the vascular tissues of embryo and pollen grains and also active in petals, sepals, caulinar leave...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Vicient CM,Gruber V,Delseny M

    更新日期:2001-07-01 00:00:00

  • Post-translational modifications of FLOWERING LOCUS C modulate its activity.

    abstract::Flowering Locus C (FLC) is a key floral repressor that precisely controls flowering time. The role of FLC has been extensively studied at the transcriptional level using molecular biological and epigenetic approaches. However, how FLC functions and how its stability is controlled at the post-translational level are on...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Kwak JS,Son GH,Song JT,Seo HS

    更新日期:2017-01-01 00:00:00

  • Characterization and functional investigation of an Arabidopsis cDNA encoding a homologue to the d-PGMase superfamily.

    abstract::An Arabidopsis thaliana cDNA (At-74) has been isolated that encoded an uncharacterized protein showing homology with members of the d-PGMase superfamily: cofactor-dependent phosphoglycerate mutases (d-PGM-ases) and the phosphatase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (6PF2Kase/F2, 6Pase). Pr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Bourgis F,Botha FC,Mani S,Hiten FN,Rigden DJ,Verbruggen N

    更新日期:2005-04-01 00:00:00

  • Isolation and characterization of a new, full-length cellulose synthase cDNA, PtrCesA5 from developing xylem of aspen trees.

    abstract::Cellulose synthases (CesA) represent a group of beta-1,4 glycosyltransferases involved in cellulose biosynthesis. Recent molecular studies confirmed that a large CesA gene family encoding various CesA isoforms exists in plants. The isolation and characterization of a new, full-length CesA cDNA, PtrCesA5 from an econom...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Kalluri UC,Joshi CP

    更新日期:2003-09-01 00:00:00

  • Mutation in xyloglucan 6-xylosytransferase results in abnormal root hair development in Oryza sativa.

    abstract::Root hairs are important for nutrient uptake, anchorage, and plant-microbe interactions. From a population of rice (Oryza sativa) mutagenized by ethyl methanesulfonate (EMS), a short root hair2 (srh2) mutant was identified. In hydroponic culture, srh2 seedlings were significantly reduced in root hair length. Bubble-li...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Wang C,Li S,Ng S,Zhang B,Zhou Y,Whelan J,Wu P,Shou H

    更新日期:2014-08-01 00:00:00

  • Drought stress modulates cuticular wax composition of the grape berry.

    abstract::Drought events are a major challenge for many horticultural crops, including grapes, which are often cultivated in dry and warm climates. It is not understood how the cuticle contributes to the grape berry response to water deficit (WD); furthermore, the cuticular waxes and the related biosynthetic pathways are poorly...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Dimopoulos N,Tindjau R,Wong DCJ,Matzat T,Haslam T,Song C,Gambetta GA,Kunst L,Castellarin SD

    更新日期:2020-05-30 00:00:00

  • Five ways to stay green.

    abstract::The relationship between carbon income and expenditure over the life of a leaf is described and related to the productivity benefits of altering the timing of senescence initiation. In genetic variants with delayed leaf senescence ('stay-greens') deconstruction of the photosynthetic apparatus during leaf senescence is...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Thomas H,Howarth CJ

    更新日期:2000-02-01 00:00:00

  • Making sense of the metabolome using evolutionary computation: seeing the wood with the trees.

    abstract::One should perhaps start off by asking the question, 'But what wood is it we want to see?' There are so many trees that make up the wood; within a post-genomics context, genes, transcripts, proteins, and metabolites are the more tangible ones. Rather than studying these components in isolation, a more holistic approac...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Goodacre R

    更新日期:2005-01-01 00:00:00

  • Overexpression of wheat Na+/H+ antiporter TNHX1 and H+-pyrophosphatase TVP1 improve salt- and drought-stress tolerance in Arabidopsis thaliana plants.

    abstract::Transgenic Arabidopsis plants overexpressing the wheat vacuolar Na(+)/H(+) antiporter TNHX1 and H(+)-PPase TVP1 are much more resistant to high concentrations of NaCl and to water deprivation than the wild-type strains. These transgenic plants grow well in the presence of 200 mM NaCl and also under a water-deprivation...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Brini F,Hanin M,Mezghani I,Berkowitz GA,Masmoudi K

    更新日期:2007-01-01 00:00:00

  • Cellulose and lignin biosynthesis is altered by ozone in wood of hybrid poplar (Populus tremula × alba).

    abstract::Wood formation in trees is a dynamic process that is strongly affected by environmental factors. However, the impact of ozone on wood is poorly documented. The objective of this study was to assess the effects of ozone on wood formation by focusing on the two major wood components, cellulose and lignin, and analysing ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Richet N,Afif D,Huber F,Pollet B,Banvoy J,El Zein R,Lapierre C,Dizengremel P,Perré P,Cabané M

    更新日期:2011-06-01 00:00:00

  • Differential coupling of gibberellin responses by Rht-B1c suppressor alleles and Rht-B1b in wheat highlights a unique role for the DELLA N-terminus in dormancy.

    abstract::During the Green Revolution, substantial increases in wheat (Triticum aestivum) yields were realized, at least in part, through the introduction of the Reduced height (Rht)-B1b and Rht-D1b semi-dwarfing alleles. In contrast to Rht-B1b and Rht-D1b, the Rht-B1c allele is characterized by extreme dwarfism and exceptional...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Van De Velde K,Chandler PM,Van Der Straeten D,Rohde A

    更新日期:2017-01-01 00:00:00

  • Good and bad protons: genetic aspects of acidity stress responses in plants.

    abstract::Physiological aspects of acidity stress in plants (synonymous with H(+) rhizotoxicity or low-pH stress) have long been a focus of research, in particular with respect to acidic soils where aluminium and H(+) rhizotoxicities often co-occur. However, toxic H(+) and Al(3+) elicit different response mechanisms in plants, ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Shavrukov Y,Hirai Y

    更新日期:2016-01-01 00:00:00

  • A methylation status analysis of the apomixis-specific region in Paspalum spp. suggests an epigenetic control of parthenogenesis.

    abstract::Apomixis, a clonal plant reproduction by seeds, is controlled in Paspalum spp. by a single locus which is blocked in terms of recombination. Partial sequence analysis of the apomixis locus revealed structural features of heterochromatin, namely the presence of repetitive elements, gene degeneration, and de-regulation....

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Podio M,Cáceres ME,Samoluk SS,Seijo JG,Pessino SC,Ortiz JP,Pupilli F

    更新日期:2014-12-01 00:00:00

  • Root exudation from Hordeum vulgare in response to localized nitrate supply.

    abstract::Root proliferation as a response to exploit zones of nutrient enrichment in soil has been demonstrated for a wide range of plant species. However, the effectiveness of this as a strategy to acquire nutrients is also dependent on interactions with the soil microbial community. Specifically, C-flow from roots modifies m...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Paterson E,Sim A,Standing D,Dorward M,McDonald AJ

    更新日期:2006-01-01 00:00:00

  • Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.

    abstract::Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress. Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance. This study aimed to determine whether galactin...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Liu Y,Zhang L,Meng S,Liu Y,Zhao X,Pang C,Zhang H,Xu T,He Y,Qi M,Li T

    更新日期:2020-01-01 00:00:00

  • Isolation and functional characterization of PgTIP1, a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng.

    abstract::The suppression subtractive hybridization technique was used to identify differentially expressed genes between hormone-autotrophic and hormone-dependent Panax ginseng callus lines. A tonoplast intrinsic protein cDNA (PgTIP1) was found to be highly and specifically expressed in hormone-autotrophic ginseng cells, which...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lin W,Peng Y,Li G,Arora R,Tang Z,Su W,Cai W

    更新日期:2007-01-01 00:00:00

  • Xylem tension affects growth-induced water potential and daily elongation of maize leaves.

    abstract::Diurnal rates of leaf elongation vary in maize (Zea mays L.) and are characterized by a decline each afternoon. The cause of the afternoon decline was investigated. When the atmospheric environment was held constant in a controlled environment, and water and nutrients were adequately supplied to the soil or the roots ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Tang AC,Boyer JS

    更新日期:2008-01-01 00:00:00

  • Plasma membrane isolation from freshwater and salt-tolerant species of Chara: antibody cross-reactions and phosphohydrolase activities.

    abstract::Plasma membranes were isolated using the aqueous polymer two-phase partition method from the algae Chara corallina and Chara longifolia, algae which differ in their ability to grow in saline environments. Enrichment of plasma membrane and depletion of tonoplast relative to the microsomal fraction was monitored using p...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Faraday CD,Spanswick RM,Bisson MA

    更新日期:1996-04-01 00:00:00

  • Soil compaction and the architectural plasticity of root systems.

    abstract::Soil compaction is a serious global problem, and is a major cause of inadequate rooting and poor yield in crops around the world. Root system architecture (RSA) describes the spatial arrangement of root components within the soil and determines the plant's exploration of the soil. Soil strength restricts root growth a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Correa J,Postma JA,Watt M,Wojciechowski T

    更新日期:2019-11-18 00:00:00

  • Overproduction of SAT and/or OASTL in transgenic plants: a survey of effects.

    abstract::The last steps of cysteine biosynthesis are catalysed by a bi-enzyme complex composed of serine acetyltransferase (SAT) and cysteine synthase, also called O-acetyl-serine (thiol) lyase (OASTL). SAT is responsible for the production of O-acetyl-serine (OAS) from serine and acetyl-coenzyme A, while OASTL catalyses the f...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Sirko A,Błaszczyk A,Liszewska F

    更新日期:2004-08-01 00:00:00

  • Differential expression of putative floral genes in Pharbitis nil shoot apices cultured on glucose compared with sucrose.

    abstract::If, following an inductive treatment of 2 d of continuous darkness, shoot apices of Pharbitis nil are cultured 1 d later on White's medium supplemented with 2% sucrose, they cannot form carpels, but they can if they are cultured on 2% glucose. It was hypothesized that the differential effect of these sugars was becaus...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Parfitt D,Herbert RJ,Rogers HJ,Francis D

    更新日期:2004-10-01 00:00:00

  • Sustained enhancement of photosynthesis in coffee trees grown under free-air CO2 enrichment conditions: disentangling the contributions of stomatal, mesophyll, and biochemical limitations.

    abstract::Coffee (Coffea spp.), a globally traded commodity, is a slow-growing tropical tree species that displays an improved photosynthetic performance when grown under elevated atmospheric CO2 concentrations ([CO2]). To investigate the mechanisms underlying this response, two commercial coffee cultivars (Catuaí and Obatã) we...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: DaMatta FM,Godoy AG,Menezes-Silva PE,Martins SC,Sanglard LM,Morais LE,Torre-Neto A,Ghini R

    更新日期:2016-01-01 00:00:00

  • Interactions between cytokinin signalling and abiotic stress responses.

    abstract::Plants have evolved elaborate mechanisms for sensing and responding to sub-optimal environmental conditions. Abiotic stresses caused by these conditions trigger a wide range of local and long-distance signals which must be co-ordinated and integrated into whole-plant processes, such as development, in order for the pl...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Zwack PJ,Rashotte AM

    更新日期:2015-08-01 00:00:00

  • Glycine-rich RNA-binding proteins are functionally conserved in Arabidopsis thaliana and Oryza sativa during cold adaptation process.

    abstract::Contrary to the increasing amount of knowledge regarding the functional roles of glycine-rich RNA-binding proteins (GRPs) in Arabidopsis thaliana in stress responses, the physiological functions of GRPs in rice (Oryza sativa) currently remain largely unknown. In this study, the functional roles of six OsGRPs from rice...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Kim JY,Kim WY,Kwak KJ,Oh SH,Han YS,Kang H

    更新日期:2010-05-01 00:00:00

  • High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth.

    abstract::Heat shock transcription factors (Hsfs) are the central regulators of the heat shock (HS) stress response in all eukaryotic organisms. HsfA2 is one of the Arabidopsis class A Hsfs, and the induction of HsfA2 expression in response to HS stress is highest among all 21 Arabidopsis Hsfs. In this study, it is reported tha...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Ogawa D,Yamaguchi K,Nishiuchi T

    更新日期:2007-01-01 00:00:00