Abstract:
:Aluminium (Al)-activated citrate secretion plays an important role in Al resistance in a number of plant species, such as rice bean (Vigna umbellata). This study further characterized the regulation of VuMATE1, an aluminium-activated citrate transporter. Al stress induced VuMATE1 expression, followed by the secretion of citrate. Citrate secretion was specific to Al stress, whereas VuMATE1 expression was not, which could be explained by a combined regulation of VuMATE1 expression and Al-specific activation of VuMATE1 protein. Pre-treatment with a protein translation inhibitor suppressed VuMATE1 expression, indicating that de novo biosynthesis of proteins is required for gene expression. Furthermore, post-treatment with a protein translation inhibitor inhibited citrate secretion, indicating that post-transcriptional regulation of VuMATE1 is critical for citrate secretion. Protein kinase and phosphatase inhibitor studies showed that reversible phosphorylation was important not only for transcriptional regulation of VuMATE1 expression but also for post-translational regulation of VuMATE1 protein activity. These results suggest that citrate secretion is dependent on both transcriptional and post-transcriptional regulation of VuMATE1. Additionally, VuMATE1 promoter-β-glucuronidase fusion lines revealed that VuMATE1 expression was restricted to the root apex and was entirely Al induced, indicating the presence of cis-acting elements regulating root tip-specific and Al-inducible gene expression, which will be an important resource for genetic improvement of plant Al resistance.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Liu MY,Chen WW,Xu JM,Fan W,Yang JL,Zheng SJdoi
10.1093/jxb/ert039subject
Has Abstractpub_date
2013-04-01 00:00:00pages
1795-804issue
7eissn
0022-0957issn
1460-2431pii
ert039journal_volume
64pub_type
杂志文章abstract::The impact of sulphur limitation on the remobilization of endogenous S compounds during the rosette stage of oilseed rape, and the interactions with N availability on these processes, were examined using a long-term (34)SO(4)(2-) labelling method combined with a study of leaf senescence progression (using SAG12/Cab as...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp172
更新日期:2009-01-01 00:00:00
abstract::Carnivorous plants inhabit nutrient-poor environments, where prominent targets of prey capture are organic nitrogen and phosphorus. Some carnivorous plants also acquire carbon from their victims. A new report focusing on Utricularia, the bladderwort, demonstrates that carbon assimilated from photosynthesis is paradoxi...
journal_title:Journal of experimental botany
pub_type: 评论,杂志文章
doi:10.1093/jxb/erp349
更新日期:2010-01-01 00:00:00
abstract::The target of rapamycin (TOR) pathway has emerged as a central hub synchronizing plant growth according to the nutrient/energy status and environmental inputs. Molecular mechanisms through which TOR promotes plant growth involve the positive regulation of transcription of cell proliferation-associated genes, mRNA tran...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ery459
更新日期:2019-04-15 00:00:00
abstract::We previously identified the W-box-like-4 (Wbl-4) element (GTAGTGACTCAT), one of six Wbl elements in the BjC-P promoter of the unusual chitinase gene BjCHI1 from Brassica juncea, as the core element responsive to fungal infection. Here, we report the isolation and characterization of the cognate transcription factor i...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw240
更新日期:2016-08-01 00:00:00
abstract::We study in situ the mechanics and growth of a leaf. Young Nicotiana tabacum leaves respond to applied mechanical stress by altering both their mechanical properties and the characteristics of their growth. We observed two opposite behaviours, each with its own typical magnitude and timescale. On timescales of the ord...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw316
更新日期:2016-10-01 00:00:00
abstract::Several root anatomical phenes affect water acquisition from drying soil, and may therefore have utility in breeding more drought-tolerant crops. Anatomical phenes that reduce the metabolic cost of the root cortex ('cortical burden') improve soil exploration and therefore water acquisition from drying soil. The best e...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru162
更新日期:2014-11-01 00:00:00
abstract::Oxidative cleavage of cis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical step in the regulation of abscisic acid (ABA) synthesis in higher plants. ABA has been associated with dormancy and flower senescence, while also regulating plant adaptive responses to various environmental stresses....
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err293
更新日期:2012-01-01 00:00:00
abstract::Small signalling peptides have emerged as important cell to cell messengers in plant development and stress responses. However, only a few of the predicted peptides have been functionally characterized. Here, we present functional characterization of two members of the IDA-LIKE (IDL) peptide family in Arabidopsis thal...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx168
更新日期:2017-06-15 00:00:00
abstract::Producing more food per unit of water has never been as important as it is at present, and the demand for water by economic sectors other than agriculture will necessarily put a great deal of pressure on a dwindling resource, leading to a call for increases in the productivity of water in agriculture. This topic has b...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru040
更新日期:2014-11-01 00:00:00
abstract::In the present study abscisic acid-induced stomatal closing, and malate effects on stomatal apertures were analysed in the presence of guard cell ion channel regulators. A recent study has suggested that abscisic acid (ABA) activation of protein kinases and/or inhibition of protein phosphatases may be central to activ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/48.Special_Issue.539
更新日期:1997-03-01 00:00:00
abstract::An experiment was designed to apply a controlled bending to a tomato stem and simultaneously to measure its effect on stem elongation. Stem elongation was measured over 2 d until steady and equal rates were obtained for the control and the treated plants. Thereafter, the basal part of the stem was submitted to a trans...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.352.1813
更新日期:2000-11-01 00:00:00
abstract::By using a fine oil-filled glass microcapillary mounted on a micromanipulator, the solutes of individual plant cells can be sampled. These samples can then be analysed using a range of physical and chemical methods. Hydrostatic pressure (cell pressure probe), osmotic pressure (picolitre osmometer), organic solutes (en...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:
更新日期:2001-04-01 00:00:00
abstract::Classical and recently found phytohormones play an important role in plant growth and development, but plants additionally control these processes through small signalling peptides. Over 1000 potential small signalling peptide sequences are present in the Arabidopsis genome. However, to date, a mere handful of small s...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ert283
更新日期:2013-12-01 00:00:00
abstract::In this study, the uptake of Na+ into the cytosol of rice (Oryza sativa L. cvs Pokkali and BRRI Dhan29) protoplasts was measured using the acetoxy methyl ester of the fluorescent sodium-binding benzofuran isopthalate, SBFI-AM, and fluorescence microscopy. By means of inhibitor analyses the mechanisms for uptake and se...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri312
更新日期:2005-12-01 00:00:00
abstract::The aim of this study is to understand the parameters regulating calcium ion distribution in leaves. Accumulation of ions in leaf tissue is in part dependent on import from the xylem. This import via the transpiration stream is more important for ions such as calcium that are xylem but not phloem mobile and cannot the...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern279
更新日期:2009-01-01 00:00:00
abstract::We initially observed that Brassica rapa cultivars containing the Turnip mosaic virus (TuMV) resistance gene, Rnt1-1, accumulated a high level of endogenous ascorbic acid (AS) and dehydroascobic acid (DHA) when infected with TuMV. We here hypothesized a possible contribution of an elevated level of AS+DHA (TAA) to the...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw223
更新日期:2016-07-01 00:00:00
abstract::The P1B ATPase heavy metal ATPase 4 (HMA4) is responsible for zinc and cadmium translocation from roots to shoots in Arabidopsis thaliana. It couples ATP hydrolysis to cytosolic domain movements, enabling metal transport across the membrane. The detailed mechanism of metal permeation by HMA4 through the membrane remai...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery353
更新日期:2019-01-01 00:00:00
abstract::Heterotrimeric G-proteins have been implicated in having a role in many plant signalling pathways. To understand further the role of G-proteins, a preliminary experiment was performed to assess the impact of the G alpha subunit loss-of-function mutation gpa1-1 on the Arabidopsis transcriptome. The analysis indicated t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp060
更新日期:2009-01-01 00:00:00
abstract::A cDNA clone encoding a 64-amino acid type 3 metallothionein protein, designated GhMT3a, was isolated from cotton (Gossypium hirsutum) by cDNA library screening. Northern blot analysis indicated that mRNA accumulation of GhMT3a was up-regulated not only by high salinity, drought, and low temperature stresses, but also...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern291
更新日期:2009-01-01 00:00:00
abstract::Sugars regulate the expression of many genes at the transcriptional level. In Arabidopsis thaliana, sugars induce or repress the expression of >1800 genes, including the STP1 (SUGAR TRANSPORTER PROTEIN 1) gene, which encodes an H(+)/monosaccharide cotransporter. STP1 transcript levels decrease more rapidly after the a...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru394
更新日期:2015-01-01 00:00:00
abstract::Proanthocyanidins (PAs) are agronomically important biopolymers in higher plants composed primarily of catechin and epicatechin units. The biosynthesis of these natural products is regulated by transcription factors including proteins of the R2R3MYB class. To gain insight into the genetic control of the catechin and e...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq344
更新日期:2011-01-01 00:00:00
abstract::ERECTA family genes encode leucine-rich repeat receptor-like kinases that control multiple aspects of plant development such as elongation of aboveground organs, leaf initiation, development of flowers, and epidermis differentiation. These receptors have also been implicated in responses to biotic and abiotic stress, ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers305
更新日期:2012-11-01 00:00:00
abstract::Actin filament (AF) organization was studied during the plasmolytic cycle in leaf cells of Chlorophyton comosum Thunb. In most cells the hyperosmotic treatment induced convex or concave plasmolysis and intense reorganization of the AF cytoskeleton. Thin cortical AFs disappeared and numerous cortical, subcortical and e...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erf018
更新日期:2002-08-01 00:00:00
abstract::Fruit ripening is a complex physiological process in plants whereby cell wall programmed changes occur mainly to promote seed dispersal. Cell wall modification also directly regulates the textural properties, a fundamental aspect of fruit quality. In this study, two full-sib populations of apple, with 'Fuji' as the co...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err326
更新日期:2012-02-01 00:00:00
abstract::The complete elimination of unwanted cells during development is a repeated theme in both multicellular animals and in plants. In plants, such events have been extensively studied and reviewed in terms of their molecular regulation, of marker genes and proteins expressed, and in terms of cellular changes associated wi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw364
更新日期:2017-02-01 00:00:00
abstract::Populus euphratica is a poplar species growing in arid regions of Central Asia, where its distribution remains nevertheless restricted to river-banks or to areas with an access to deep water tables. To test whether the hydraulic architecture of this species differs from that of other poplars with respect to this ecolo...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri198
更新日期:2005-08-01 00:00:00
abstract::Pectin is a normal constituent of cell walls of green plants. When supplied externally to live cells or walls isolated from the large-celled green alga Chara corallina, pectin removes calcium from load-bearing cross-links in the wall, loosening the structure and allowing it to deform more rapidly under the action of t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers089
更新日期:2012-06-01 00:00:00
abstract::Cell wall changes were examined in fruit of a melting flesh peach (Prunus persica L.) allowed to ripen on the tree. Three phases to softening were noted, the first of which began prior to the completion of flesh colour change and an increase in ethylene evolution. Softening in young mature fruit, prior to ripening, wa...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh227
更新日期:2004-09-01 00:00:00
abstract::Nitrogen (N) nutrition, post-anthesis temperature and drought-induced changes in the kinetics of accumulation of dry mass, total grain N and protein fractions (albumins-globulins, amphiphils, gliadins, and glutenins) contents were examined for winter wheat (Triticum aestivum L.). Crops were grown in controlled environ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg183
更新日期:2003-07-01 00:00:00
abstract::Iron is an essential element for all living organisms due to its ubiquitous role in redox and other enzymes, especially in the context of respiration and photosynthesis. The iron uptake and storage systems of terrestrial/higher plants are now reasonably well understood, with two basic strategies for iron uptake being ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers225
更新日期:2012-10-01 00:00:00