Abstract:
:The three primary tissue systems of the funiculus each undergo unique developmental programs to support the growth and development of the filial seed. To understand the underlying transcriptional mechanisms that orchestrate development of the funiculus at the globular embryonic stage of seed development, we used laser microdissection coupled with RNA-sequencing to produce a high-resolution dataset of the mRNAs present in the epidermis, cortex, and vasculature of the Brassica napus (canola) funiculus. We identified 7761 additional genes in these tissues compared with the whole funiculus organ alone using this technology. Differential expression and enrichment analyses were used to identify several biological processes associated with each tissue system. Our data show that cell wall modification and lipid metabolism are prominent in the epidermis, cell growth and modification occur in the cortex, and vascular tissue proliferation and differentiation occur in the central vascular strand. We provide further evidence that each of the three tissue systems of the globular stage funiculus are involved in specific biological processes that all co-ordinate to support seed development. The identification of genes and gene regulators responsible for tissue-specific developmental processes of the canola funiculus now serves as a valuable resource for seed improvement research.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Chan AC,Khan D,Girard IJ,Becker MG,Millar JL,Sytnik D,Belmonte MFdoi
10.1093/jxb/erw179subject
Has Abstractpub_date
2016-05-01 00:00:00pages
3561-71issue
11eissn
0022-0957issn
1460-2431pii
erw179journal_volume
67pub_type
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