Abstract：
BACKGROUND & AIMS:Caspases are critical mediators of apoptosis and proliferation of peripheral blood T cells (PBT), but their role in lamina propria T cells (LPT), a cell population highly susceptible to apoptosis, has not been explored. METHODS:RA(+), RO(+) PBT, and LPT were activated with CD3, CD2, and CD28 antibodies, and caspase activity, apoptosis, and proliferation were measured by a fluorometric assay, DNA content, and thymidine incorporation, respectively. Levels of FLIP, an endogenous inhibitor of caspase 8, were measured by immunoblotting. RESULTS:In RA(+) and RO(+) PBT, activation leads to significant increase of caspase activity but not cell death, whereas in LPT a lower elevation of caspase activity was followed by a marked degree of apoptosis. Based on the results of its inhibition, caspase 8 seemed to be essential for LPT apoptosis but, in contrast to RA(+) PBT, had no effect on proliferation. In addition, compatible with their differential susceptibility to apoptosis, levels of FLIP were lower in LPT than PBT. CONCLUSIONS:The high susceptibility of LPT to apoptosis is associated with a distinct regulation of caspase 8 activity, which seems to reflect their mucosal origin rather than simply their memory status. This unique behavior may allow proper control of mucosal T-cell proliferation while still permitting elimination by apoptosis in the face of excessive antigenic pressure.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Sturm A,Mohr S,Fiocchi Cdoi
10.1053/gast.2002.32996keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
1334-45issue
5eissn
0016-5085issn
1528-0012pii
S0016508502367672journal_volume
122pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract：:Intracellular microelectrodes were used to evaluate electrical properties of the cell membranes in Necturus antral mucosa during exposure to luminal acid alone (pH 4) or to 5 mmol/L aspirin [acetylsalicylic acid (ASA)] in the presence of luminal acid. When nutrient solutions were buffered by HCO3- (pH 7.3), ASA modera...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(92)91167-3
更新日期：1992-11-01 00:00:00
abstract：:Bile acids cause diarrhea by inducing colonic secretion, probably mediated through the cyclic AMP system. The aim was to determine the effects of an adenylate cyclase inhibitor, propranolol, on deoxycholic acid (DCA) stimulation of net secretion and the cyclic AMP system in the colon. In each of 30 New Zealand white r...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1976-07-01 00:00:00
abstract：:A patient with gastric outlet syndrome (Bouveret's syndrome) caused by a large gallstone impacted in the duodenal bulb was successfully treated by extracorporeal shock-wave lithotripsy. Thus, open abdominal surgery could be avoided. For disintegration of the stone, three consecutive lithotripsy procedures were necessa...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(89)90086-3
更新日期：1989-08-01 00:00:00
abstract：BACKGROUND/AIMS:The elevation of cytosolic free calcium concentration ([Ca2+]i) and intracellular pH mediate the growth factor-initiated proliferation of many cells, but it is not known if they trigger mitosis in resting hepatocytes. The maintenance of [Ca2+]i and intracellular pH depends partly on extracellular calciu...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(95)90077-2
更新日期：1995-02-01 00:00:00
abstract：BACKGROUND & AIMS:Although bacterial bowel flora may be one of the contributing factors in the pathogenesis of chronic mucosal inflammation, antibiotic treatment has no established role in ulcerative colitis. The aim of the study was to evaluate the role of ciprofloxacin in the induction and maintenance of remission in...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0016-5085(98)70076-9
更新日期：1998-11-01 00:00:00
abstract：:Experiments were designed to test the hypothesis that alterations of intestinal motility accompany enteric helminth infections. Motility was measured in rats during the intestinal phase of infection with the nematode Trichinella spiralis by following the transit of radioactive chromium through the gut. Intraduodenal c...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:The fibrinolytic activity of blood draining from the stomachs of patients with gastroduodenal disease has been compared with the fibrinolytic activity of blood in the systemic venous circulation and with the blood draining from normal stomachs. Gastric venous blood from normal and diseased stomachs contains greater am...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：BACKGROUND & AIMS:Dilutional hyponatremia is a frequent complication of cirrhosis partly because of nonosmotic vasopressin release. No effective therapy exists for this complication. Therefore, we investigated the effects of VPA-985, an orally active vasopressin V2 receptor antagonist, in patients with cirrhosis and di...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1053/gast.2003.50143
更新日期：2003-04-01 00:00:00
abstract：BACKGROUND & AIMS:Impaired gallbladder emptying occurs in patients undergoing bile salt therapy for cholesterol gallstone dissolution and in patients with cirrhosis who have elevated serum bile salt concentrations. To determine if bile salts directly inhibit gallbladder contractility, isometric contraction of the guine...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(97)70053-2
更新日期：1997-05-01 00:00:00
abstract：BACKGROUND & AIMS:Although protein kinase C (PKC) alterations may play a role in the abnormal reactivity of cirrhotic rat aortas, its isoforms and cellular distribution are unknown. We therefore studied the protein expression and cellular distribution of PKC isoforms and their activation in cirrhotic rat aortas. METHO...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2000.8522
更新日期：2000-07-01 00:00:00
abstract：BACKGROUND & AIMS:Microsatellite instability is a property of most tumors occurring in the context of hereditary nonpolyposis colon cancer. Instability also occurs in 10%-15% of apparently sporadic colorectal cancers, and it has been hypothesized that this instability may indicate a genetic predisposition to colonic ca...
journal_title：Gastroenterology
pub_type： 评论,杂志文章
doi：10.1016/0016-5085(95)90742-4
更新日期：1995-12-01 00:00:00
abstract：BACKGROUND AND AIMS:Tumor necrosis factor (TNF) is a cytokine that promotes inflammation and contributes to pathogenesis of inflammatory bowel diseases. Unlike other cells and tissues, intestinal epithelial cells undergo rapid cell death upon exposure to TNF, by unclear mechanisms. We investigated the roles of inhibito...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2016.11.019
更新日期：2017-03-01 00:00:00
abstract：:Assessment of the influence of nutritional substrates on the disposition of ingested ethanol from the blood requires, first, an understanding of the influence that these may exert upon ethanol absorption from the gastrointestinal tract. In this report, the effects of various carbohydrates on the intestinal absorption ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：:This study examines restitution of the gastric surface epithelium at luminal pH 1 after damage to the gastric mucosa by instillation of 2 M NaCl for 10 min. After mucosal damage, potential difference decreased to zero and H+ back-diffusion increased. Gastric blood flow increased after exposure to 2 M NaCl, irrespectiv...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(87)90437-9
更新日期：1987-10-01 00:00:00
abstract：BACKGROUND AND AIMS:The peptidyl prolyl isomerase Pin1 frequently is overexpressed in hepatocellular carcinoma (HCC). Hepatitis B virus (HBV) is the most common etiologic agent in HCC, and its encoded X-protein (HBx) is oncogenic and possesses a serine-proline motif that may bind Pin1. The role of Pin1 in hepatocarcino...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2006.12.030
更新日期：2007-03-01 00:00:00
abstract：BACKGROUND & AIMS:Hereditary diffuse gastric cancer (HDGC) families carry CDH1 heterozygous germline mutations; their tumors acquire complete CDH1 inactivation through "2nd-hit" mechanisms. Most frequently, this occurs via promoter hypermethylation (epigenetic modification), and less frequently via CDH1 mutations and l...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2009.02.065
更新日期：2009-06-01 00:00:00
abstract：BACKGROUND AND AIMS:Cholestasis is one of the principal manifestations of liver disease and often results from disorders involving bile duct epithelia rather than hepatocytes. A range of disorders affects biliary epithelia, and no unifying pathophysiologic event in these cells has been identified as the cause of choles...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(03)01201-0
更新日期：2003-10-01 00:00:00
abstract：BACKGROUND & AIMS:In several rat infection experiments, we have shown that dietary calcium inhibits intestinal colonization and translocation of invasive salmonella. The aim of the present study was to find out whether calcium is also protective against enterotoxigenic Escherichia coli (ETEC) infection. This was first ...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章
doi：10.1016/s0016-5085(03)00884-9
更新日期：2003-08-01 00:00:00
abstract：:Three analogues of the carboxyl-terminal tricosapeptide of secretin (S5-27), one with glutamine replacing glutamic acid in position 9 (9-Gln-S5-27), a second with asparagine substituted for aspartic acid in position 15 (15-Asn-S5-27), and a third with both replacements (9-Gln-15-Asn-S5-27) were tested for their abilit...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1976-12-01 00:00:00
abstract：:The prevalence of gastroesophageal varices and gastrointestinal hemorrhage was determined in 124 patients with severe chronic active liver disease (CALD) receiving prednisone and followed regularly for up to 10 yr. Varices were demonstrated by contrast radiography in only 19 patients (15%). Ten had varices before ther...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:This study was conducted to determine whether short-term (1.75 h) luminal glucose perfusion increases the mucosal protein synthesis rate in rat small intestine. A luminal perfusate containing 56 mM glucose was compared with a control perfusate containing mannitol in two jejunal segments constructed in the same animal....
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：BACKGROUND:The composition of the extracellular matrix (ECM) as well as insulinlike growth factor I (IGF-I) receptor density vary along the crypt-villus axis. We determined whether components of the ECM influence IGF-I receptor expression in IEC-18 rat small intestine crypt cells. METHODS:IEC-18 cells were cultured on...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(93)90649-w
更新日期：1993-06-01 00:00:00
abstract：:Human intrinsic factor (IF) is found on the surface of parietal cells at the light microscopic level by immunofluorescence, but there is no direct evidence that IF is synthesized in and secreted from this cell. In order to provide this evidence, IF was localized at the ultrastructural level in human stomach, using an ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
abstract：:Enteroglucagon-containing cells have been maintained in short-term culture, and the morphologic characteristics of these cells and their response to selected agents have been determined. After 48 h in culture the ultrastructural appearance of the enteroglucagon-immunoreactive cells showed evidence of polarization with...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(87)90442-2
更新日期：1987-10-01 00:00:00
abstract：BACKGROUND & AIMS:Abdominal pain is the main symptom in patients with irritable bowel syndrome (IBS). Glycerol is an irritant of colonic mucosa. The aim of this study was to evaluate the effects of an intraluminal injection of glycerol on colonic motility and tone in patients with IBS and their modulation by drugs. ME...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1053/gast.1996.v110.pm8566580
更新日期：1996-02-01 00:00:00
abstract：:Disordered gustatory acuity was demonstrated in 22 patients with acute viral hepatitis and in 16 patients with chronic liver disease utilizing subjective responses and objective measurements of detection and recognition thresholds and scaling for NaCl, sucrose, HCl, and urea. In patients with early hepatitis and those...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：BACKGROUND & AIMS:Nitric oxide is a major inhibitory neurotransmitter in the enteric nervous system. Loss or dysfunction of nitrinergic neurons is associated with serious disruptions of motility, intractable symptoms, and long-term suffering. The aim of this study was to evaluate the effect of intrapyloric transplantat...
journal_title：Gastroenterology
pub_type： 评论,杂志文章
doi：10.1053/j.gastro.2005.08.055
更新日期：2005-12-01 00:00:00
abstract：:The range of activity and the location of lipase and pepsin were determined in the stomach and duodenum of infants, children, and adults. The range of lipase activity in biopsy specimens from the gastric body, in 29 subjects aged from 3 months to 26 years, was 1.8-5.3 U/mg protein (1 U is 1 mumol [3H]oleic acid releas...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(91)90467-y
更新日期：1991-07-01 00:00:00
abstract：BACKGROUND & AIMS:The mechanism by which gastric mucosa becomes more resistant to damage by repeated aspirin administration is not known. Transforming growth factor alpha (TGF-alpha) and epidermal growth factor (EGF) prevent drug-induced gastric injury. The aim of this study was to determine whether gastroduodenal tiss...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.1996.v110.pm8613050
更新日期：1996-05-01 00:00:00
abstract：BACKGROUND & AIMS:Crohn's disease (CD) is an inflammatory bowel disease characterized by uncontrolled immune responses to bacterial flora, with excessive activation of T lymphocytes. MICA is a stress-induced major histocompatibility complex-related molecule expressed on normal intestinal epithelial cells (IECs) and rec...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2007.03.025
更新日期：2007-06-01 00:00:00
