Molecular specialization of breast vasculature: a breast-homing phage-displayed peptide binds to aminopeptidase P in breast vasculature.

abstract：:Mutations in the thyroid hormone receptor α 1 gene (THRA) have recently been identified as a cause of intellectual deficit in humans. Patients present with structural abnormalities including microencephaly, reduced cerebellar volume and decreased axonal density. Here, we show that directed differentiation of THRA muta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krieger TG,Moran CM,Frangini A,Visser WE,Schoenmakers E,Muntoni F,Clark CA,Gadian D,Chong WK,Kuczynski A,Dattani M,Lyons G,Efthymiadou A,Varga-Khadem F,Simons BD,Chatterjee K,Livesey FJ

更新日期：2019-11-05 00:00:00

abstract：:Efforts to delineate the advent of many enzymes essential to protein translation are often limited by the fact that the modern genetic code evolved before divergence of the tree of life. Glutaminyl-tRNA synthetase (GlnRS) is one noteworthy exception to the universality of the translation apparatus. In eukaryotes and s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skouloubris S,Ribas de Pouplana L,De Reuse H,Hendrickson TL

更新日期：2003-09-30 00:00:00

abstract：:Expression of amino acid biosynthesis genes in bacteria is often repressed when abundant supplies of the cognate amino acid are available. Repression of the Bacillus subtilis lysC gene by lysine was previously shown to occur at the level of premature termination of transcription. In this study we show that lysine dire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grundy FJ,Lehman SC,Henkin TM

更新日期：2003-10-14 00:00:00

abstract：:Locus content maps are derived from monosomic or disomic chromosomes broken by radiation, shearing, or other clastogen, the fragments being distributed among clones by dilution or incorporation into the cells of another species and scored for segregation of markers. Locus content maps provide evidence about radiosensi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teague JW,Collins A,Morton NE

更新日期：1996-10-15 00:00:00

abstract：:In order to determine the chemical features of human placental lactogen (hPL) necessary for its biologic activity we prepared the following fragments from the plasmin-cleaved hormone: reduced and alkylated 1--134, reduced and alkylated 141--191, and a 1--134 dimer joined through the single cysteinyl residue at positio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Russell J,Sherwood LM,Kowalski K,Schneider AB

更新日期：1979-03-01 00:00:00

abstract：:The eukaryotic DNA sliding clamp that keeps DNA polymerase engaged at a replication fork, called proliferating cell nuclear antigen (PCNA), is loaded onto the 3' ends of primer DNA through its interaction with a heteropentameric protein complex called replication factor C (RFC). The ATPase activity of RFC is necessary...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shiomi Y,Usukura J,Masamura Y,Takeyasu K,Nakayama Y,Obuse C,Yoshikawa H,Tsurimoto T

更新日期：2000-12-19 00:00:00

abstract：:We investigate the hypothesis that two classes of Ca2+ currents, one quickly inactivated by Ca2+ and one slowly inactivated by voltage, contribute to bursting electrical activity in pancreatic islets. A mathematical model of these currents is fit to the experimental whole-cell current-voltage and inactivation profiles...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keizer J,Smolen P

更新日期：1991-05-01 00:00:00

abstract：:Soil bacteria and fungi play key roles in the functioning of terrestrial ecosystems, yet our understanding of their responses to climate change lags significantly behind that of other organisms. This gap in our understanding is particularly true for drylands, which occupy ∼41% of Earth´s surface, because no global, sy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maestre FT,Delgado-Baquerizo M,Jeffries TC,Eldridge DJ,Ochoa V,Gozalo B,Quero JL,García-Gómez M,Gallardo A,Ulrich W,Bowker MA,Arredondo T,Barraza-Zepeda C,Bran D,Florentino A,Gaitán J,Gutiérrez JR,Huber-Sannwald E,Jan

更新日期：2015-12-22 00:00:00

abstract：:We have found a defect in the ouabain-sensitive Na+, K+-ATPase (Na+ pump, EC 3.6.1.37) of erythrocytes from streptozocin diabetic rats. This defect was accompanied by an increase in cell volume and osmotic fragility and a decrease in the cytosolic K+/Na+ ratio. There was also a doubling in the time needed for diabetic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kowluru R,Bitensky MW,Kowluru A,Dembo M,Keaton PA,Buican T

更新日期：1989-05-01 00:00:00

abstract：:Selective inhibition of the intrinsic coagulation pathway is a promising strategy for developing safer anticoagulants that do not cause serious bleeding. Intrinsic tenase, the final and rate-limiting enzyme complex in the intrinsic coagulation pathway, is an attractive but less explored target for anticoagulants due t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao L,Wu M,Xiao C,Yang L,Zhou L,Gao N,Li Z,Chen J,Chen J,Liu J,Qin H,Zhao J

更新日期：2015-07-07 00:00:00

abstract：:The generalized seizures of status epilepticus (SE) trigger a series of molecular and cellular events that produce cognitive deficits and can culminate in the development of epilepsy. Known early events include opening of the blood-brain barrier (BBB) and astrocytosis accompanied by activation of brain microglia. Wher...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Varvel NH,Neher JJ,Bosch A,Wang W,Ransohoff RM,Miller RJ,Dingledine R

更新日期：2016-09-20 00:00:00

abstract：:Paired immunoglobulin-like type 2 receptor α (PILRα) and β (PILRβ) belong to the PILR family and are related to innate immune regulation in various species. Despite their high sequence identity, PILRα and PILRβ are shown to have variant sialic acid (SA) binding avidities. To explore the molecular basis of this interac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu Q,Lu G,Qi J,Wang H,Xuan Y,Wang Q,Li Y,Zhang Y,Zheng C,Fan Z,Yan J,Gao GF

更新日期：2014-06-03 00:00:00

abstract：:Dimers of bacteriochlorophyll a (Bchla) with optical absorption maximum at 853 nm and a nonconservative circular dichroism spectrum are formed in a solution of formamide/water that contains micelles of Triton X-100. The apparent equilibrium constant and the corresponding Gibbs energy change for the Bchl self-organizat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scherz A,Rosenbach-Belkin V

更新日期：1989-03-01 00:00:00

abstract：:The in vivo production of frameshift and base-substitution mutations predicted as a consequence of the metabolic processing of misaligned quasipalindromic DNA sequences has been confirmed. Spontaneous frameshift mutations of the T4 rII gene that had been genetically mapped to quasipalindromic DNA sequences were sequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Boer JG,Ripley LS

更新日期：1984-09-01 00:00:00

abstract：:People who are more avoidant of pathogens are more politically conservative, as are nations with greater parasite stress. In the current research, we test two prominent hypotheses that have been proposed as explanations for these relationships. The first, which is an intragroup account, holds that these relationships ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tybur JM,Inbar Y,Aarøe L,Barclay P,Barlow FK,de Barra M,Becker DV,Borovoi L,Choi I,Choi JA,Consedine NS,Conway A,Conway JR,Conway P,Adoric VC,Demirci DE,Fernández AM,Ferreira DC,Ishii K,Jakšić I,Ji T,van Leeuwen

更新日期：2016-11-01 00:00:00

abstract：:The recent development of HIV-1 lentiviral vectors is especially useful for gene transfer because they achieve efficient integration into nondividing cell genomes and successful long-term expression of the transgene. These attributes make the vector useful for gene delivery, mutagenesis, and other applications in mamm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai Z,Han I,Park M,Brady RO

更新日期：2002-03-19 00:00:00

abstract：:Insulin and insulin-like growth factor 1 (IGF1) are ubiquitous growth factors that regulate proliferation in most mammalian tissues including pancreatic islets. To explore the specificity of insulin receptors in compensatory beta-cell growth, we examined two models of insulin resistance. In the first model, we used li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okada T,Liew CW,Hu J,Hinault C,Michael MD,Krtzfeldt J,Yin C,Holzenberger M,Stoffel M,Kulkarni RN

更新日期：2007-05-22 00:00:00

abstract：:In most organisms, light plays a key role in the synchronization of the circadian timing system with the environmental day-night cycle. Light pulses that phase-shift the circadian clock also induce the expression of period (per) genes in vertebrates. Here, we report the cloning of a zebrafish per gene, zfper4, which i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vallone D,Gondi SB,Whitmore D,Foulkes NS

更新日期：2004-03-23 00:00:00

abstract：:Phosphorylation of inositol phospholipids plays a key role in cellular regulation via the generation of intracellular second messengers. In addition, it represents a mechanism to regulate interactions of the lipid bilayer with proteins and protein scaffolds involved in vesicle budding, cytoskeletal organization, and s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo J,Wenk MR,Pellegrini L,Onofri F,Benfenati F,De Camilli P

更新日期：2003-04-01 00:00:00

abstract：:The patch-clamp technique allows currents to be recorded through single ion channels in patches of cell membrane in the tips of glass pipettes. When recording, voltage is typically applied across the membrane patch to drive ions through open channels and to probe the voltage-sensitivity of channel activity. In this st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gil Z,Silberberg SD,Magleby KL

更新日期：1999-12-07 00:00:00

abstract：:Mass cytometry enables an unprecedented number of parameters to be measured in individual cells at a high throughput, but the large dimensionality of the resulting data severely limits approaches relying on manual "gating." Clustering cells based on phenotypic similarity comes at a loss of single-cell resolution and o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shekhar K,Brodin P,Davis MM,Chakraborty AK

更新日期：2014-01-07 00:00:00

abstract：:Reports differ as to whether reconstitution of telomerase activity alone is sufficient for immortalization of different types of human somatic cells or whether additional activities encoded by other "immortalizing" genes are also required. Here we show that ectopic expression of either the catalytic subunit of human t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Hare MJ,Bond J,Clarke C,Takeuchi Y,Atherton AJ,Berry C,Moody J,Silver AR,Davies DC,Alsop AE,Neville AM,Jat PS

更新日期：2001-01-16 00:00:00

abstract：:The electromechanochemical model has been reformulated to take account of the close connection between energy coupling and catalysis. In catalysis the protein is programmed to utilize thermal energy to produce local strains in the catalytic cavity and to generate conformational states that favor substrate --> product ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Green DE,Ji S

更新日期：1973-03-01 00:00:00

abstract：:The larger structures are, the lower their mechanical strength. Already discussed by Leonardo da Vinci and Edmé Mariotte several centuries ago, size effects on strength remain of crucial importance in modern engineering for the elaboration of safety regulations in structural design or the extrapolation of laboratory r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss J,Girard L,Gimbert F,Amitrano D,Vandembroucq D

更新日期：2014-04-29 00:00:00

abstract：:Endocrine therapy resistance invariably develops in advanced estrogen receptor-positive (ER+) breast cancer, but the underlying mechanisms are largely unknown. We have identified C-terminal SRC kinase (CSK) as a critical node in a previously unappreciated negative feedback loop that limits the efficacy of current ER-t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao T,Li W,Wang X,Xu H,Yang J,Wu Q,Huang Y,Geradts J,Jiang P,Fei T,Chi D,Zang C,Liao Q,Rennhack J,Andrechek E,Li N,Detre S,Dowsett M,Jeselsohn RM,Liu XS,Brown M

更新日期：2018-07-31 00:00:00

abstract：:Dantu is a rare antigen of the human MNSs blood group system carried by a hybrid glycophorin on the erythrocyte membrane. To delineate its structure and relationship to alpha and delta glycophorins at the genomic level, we analyzed DNA from a three-generation family, in which both the presence of Dantu and Mi-III (ano...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang CH,Blumenfeld OO

更新日期：1988-12-01 00:00:00

abstract：:When purified dimers of hemagglutinin-neuraminidase molecules released by protease digestion from three strains of human parainfluenza virus 1 were used in crystallization trials, long thin needle crystals formed. Electron microscopic analysis of these needle crystals revealed that they are composed of stacks of tripl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murti KG,Takimoto T,Laver WG,Portner A

更新日期：1993-02-15 00:00:00

abstract：:Crosses between closely related animal species often result in male hybrids that are sterile, and the molecular and functional basis of genetic factors for hybrid male sterility is of great interest. Here, we report a molecular and functional analysis of HMS1, a region of 9.2 kb in chromosome 3 of Drosophila mauritian...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liénard MA,Araripe LO,Hartl DL

更新日期：2016-07-19 00:00:00

abstract：:The region of the simian virus 40 genome complementary to the 5' end of the most abundant poly(A)-containing 19S and 16S mRNAs was mapped by hybridization of double-labeled RNA ([3H]methyl group and [14C]uridine) to specific DNA fragments. Chemical identification of methylated residues indicated that a common "leader"...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lavi S,Groner Y

更新日期：1977-12-01 00:00:00

abstract：:Germ-line missense mutations of the receptor-like tyrosine kinase ret are the causative genetic event of the multiple endocrine neoplasia (MEN) type 2A and type 2B syndromes and of the familial medullary thyroid carcinoma. We have used the rat pheochromocytoma cell line, PC12, as a model system to investigate the mech...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Califano D,D'Alessio A,Colucci-D'Amato GL,De Vita G,Monaco C,Santelli G,Di Fiore PP,Vecchio G,Fusco A,Santoro M,de Franciscis V

更新日期：1996-07-23 00:00:00