当前位置： SCI文献检索 > JAPANESE JOURNAL OF OPHTHALMOLOGY期刊下所有文献 > Involvement of mechanical stretch in the gelatinolytic activity of the fibrous sclera of chicks, in vitro.

Involvement of mechanical stretch in the gelatinolytic activity of the fibrous sclera of chicks, in vitro.

Abstract：

PURPOSE:To investigate the role of mechanical stretch in the regulation of matrix metalloproteinase-2 (MMP-2) in scleral fibroblasts of chick embryos. METHODS:Scleral fibroblasts derived from chick embryos were seeded onto flexible bottom culture plates, and subjected to a pulsatile stretch when the cells became subconfluent. After stretching, the cells and the conditioned medium were harvested. One portion of the conditioned medium was activated by reduction, alkylation, and 4-aminophenylmercuric acetate (APMA) treatment. The conditioned medium, with and without the treatment, was subjected to gelatin zymography and quantitative assays. Total cytoplasmic RNA was extracted from the cells, and the expression of MMP-2 and the tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA was examined by Northern blot analysis. RESULTS:The predominant gelatinolytic enzyme secreted by scleral fibroblasts was MMP-2. The mechanical stretch increased the gelatinolytic activities significantly in the conditioned medium with reduction, alkylation, and APMA treatment. Mechanical stretch also enhanced the expression of MMP-2 and TIMP-2 mRNA in scleral fibroblasts significantly. CONCLUSIONS:These results suggest that mechanical stretch may be involved in the regulation of the extracellular matrix in the fibrous sclera of chicks in vivo.

journal_name

Jpn J Ophthalmol

journal_title

Japanese journal of ophthalmology

authors

Fujikura H,Seko Y,Tokoro T,Mochizuki M,Shimokawa H

doi

10.1016/s0021-5155(01)00451-8

keywords:

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

24-30

issue

1

eissn

0021-5155

issn

1613-2246

pii

S0021515501004518

journal_volume

46

pub_type

杂志文章

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