Abstract:
PURPOSE:To determine the pharmacological response when dysiherbaine acts on cultured Müller cells, considering the glutamate receptor functions. Dysiherbaine is a new excitotoxic amino acid, which was recently isolated from the liquid extract of a certain sponge. METHODS:Retinas of adult rabbits were used to culture the Müller cells. Intracellular calcium ion concentration ([Ca(2+)](i)) analysis was done by fluorophotometry with the calcium indicator, acetoxymethyl ester fura-2 (Fura-2 AM). RESULTS:A transient increase of [Ca(2+)](i) was observed following the administration of dysiherbaine (2.5 microM-2.5 mM) to Müller cells, but no [Ca(2+)](i) increase was observed in the extracellular calcium-free solution. This increase was blocked by the non-N-methyl-D-aspartate (NMDA) glutamate receptor antagonist, 6-cyano-7 nitroquinoxaline-2,3-dione (CNQX). A dysiherbaine-induced increase in [Ca(2+)](i) following preincubation of the NMDA glutamate receptor antagonist, (5R,10S)-(+)-5-methyl-10,11-dyhydro-5H-dibenzo [a,d] cyclohepten-5, 10-imine hydrogen maleate (MK 801) was seen in the same number of Müller cells with and without the antagonist. CONCLUSIONS:Dysiherbaine appears to act primarily as a non-NMDA glutamate receptor agonist, having a secondary action as a NMDA glutamate receptor agonist.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Minei Rdoi
10.1016/s0021-5155(01)00488-9keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
153-9issue
2eissn
0021-5155issn
1613-2246pii
S0021515501004889journal_volume
46pub_type
杂志文章abstract::Lacquer cracks are thought to represent healed mechanical breaks in the retinal pigment epithelium, Bruch's membrane, and choriocapillaris complex. In this study, we analyzed the indocyanine green (ICG) angiographic features of lacquer cracks and compared them with findings using fluorescein angiography. Complete opht...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(98)00008-2
更新日期:1998-07-01 00:00:00
abstract::Distribution of microglia cells was studied in the normal retina of Wistar rats kept in a specific-pathogen-free condition, using a combined technique of light and electron microscopy with the weak silver carbonate staining method of del Rio-Hortega. Typical microglia cells were found mainly in the inner plexiform lay...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:The aqueous humor (AH) contains numerous immunosuppressive molecules that contribute to the ocular immune privilege. Here, we mimic an inflammatory environment to analyze the inhibitory effects of the AH on lipopolysaccharide (LPS)-induced maturation of dendritic cells (DC). METHODS:Different concentrations of...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-011-0060-0
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-003-0004-4
更新日期:2004-01-01 00:00:00
abstract:PURPOSE:To evaluate the repeatability of corneal deformation parameters measured using a dynamic Scheimpflug analyzer and the impact of baseline clinical factors on the repeatability of each parameter. STUDY DESIGN:Retrospective, cross-sectional study. METHODS:Forty-eight eyes (48 healthy subjects; mean age, 49.0 ± 1...
journal_title:Japanese journal of ophthalmology
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1007/s10384-010-0812-2
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abstract:PURPOSE:The mortality rate of patients with retinoblastoma still varies in developing countries, and data for Asia and Taiwan are relatively scarce. In this retrospective study, we aimed to describe the survival characteristics and prognostic factors of 54 retinoblastoma cases diagnosed at the Taipei Veterans General H...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-005-0320-y
更新日期:2006-05-01 00:00:00
abstract:PURPOSE:To evaluate changes in conjunctival-scleral thickness following strabismus surgery with anterior segment optical coherence tomography (AS-OCT). STUDY DESIGN:Prospective, observational, consecutive case series. METHODS:Distances between the conjunctival epithelium and inner scleral wall were measured with AS-O...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-018-0609-2
更新日期:2018-09-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00233-1
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journal_title:Japanese journal of ophthalmology
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doi:10.1007/s10384-018-0589-2
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(97)00046-4
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(03)00144-8
更新日期:2003-11-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-03-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
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更新日期:2019-11-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-013-0239-7
更新日期:2013-07-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00157-x
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
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更新日期:2009-09-01 00:00:00
abstract::The proliferative activity of the lens epithelium in the early stages of cataract crisis was investigated in rats and mice using the 3H-thymidine autoradiographic method with the whole-mount preparations of total lens epithelial cells. Three-week-old Sprague-Dawley (SD) rats were given a diet which included galactose ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-005-0289-6
更新日期:2006-03-01 00:00:00
abstract::A nationwide, multicenter study of typical retinitis pigmentosa was carried out in collaboration with 13 university hospitals throughout Japan. A total of 253 patients, 122 males and 131 females, with a wide range of ages (mean 48 years), were registered during a two-month period in 1989. Determination of inheritance ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:
更新日期:1993-01-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,评审
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(01)00451-8
更新日期:2002-01-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Astrocytes were cultured from the cerebral cortices of newborn rabbits and were so confirmed by staining of the glial fibrillary acidic protein (GFAP). In Experiment I, the astrocytes of newborn rabbits of both sexes from one mother were pooled and suspended in a culture medium, and approximately 1-2 X 10(4) cells wer...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-008-0568-0
更新日期:2008-09-01 00:00:00