Abstract：
:A rapid and accurate method to quantify ochratoxin A (OTA) at ppt (pg/ml) levels in urine has been developed. The method uses commercial immunoaffinity columns for clean-up and reversed phase high-performance liquid chromatography (HPLC) with fluorescence detector for quantification of the toxin. Average recoveries of OTA from human urine spiked at levels from 0.05 ng/ml to 1.0 ng/ml ranged from 88% to 93%, with relative standard deviations (RSDs) between 1% and 8%. Detection limit was 0.005 ng/ml. Out of 41 human urine samples, 25 were found positive to OTA with only one sample exceeding 0.05 ng/ml; the latter originated from a patient affected by karyomegalic interstitial nephritis. The method can be used as a rapid and non-invasive tool to assess human and animal exposure to OTA in epidemiological studies and to establish the possible role of OTA in acute animal intoxications or human end-stage renal diseases.
journal_name
Mycopathologiajournal_title
Mycopathologiaauthors
Pascale M,Visconti Adoi
10.1023/a:1012463227948keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
91-5issue
2eissn
0301-486Xissn
1573-0832journal_volume
152pub_type
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