Abstract：
:A stainless steel paper-embedded biofilm reactor (PEBR) was developed for Candida spp. growth, permitting confluent distribution of nutrients by capillary diffusion through ordinary laboratory filter paper. Antibiogram disks were distributed along the filter paper rim, and the PEBR received 0.1 or 0.01 % crystal violet (CV) at 200 μL min(-1) and at 37 °C, for 48 h. CV was recovered from the disks and measured at 540 nm. Candida albicans SC5314 cells were applied onto antibiogram disks. The bioreactor was assembled, and YEPD broth was admitted (200 μL min(-1)) at 37 °C, for 72 h. Biofilm growth was estimated via the MTT reduction test. Controls were disks that received the same treatments, except for the fungus. The PEBR was considered high-throughput table, low-cost, and feasible to grow C. albicans biofilms.
journal_name
Mycopathologiajournal_title
Mycopathologiaauthors
Selow ML,Rymovicz AU,Ribas CR,Saad RS,Rosa RT,Rosa EAdoi
10.1007/s11046-015-9889-ysubject
Has Abstractpub_date
2015-08-01 00:00:00pages
27-33issue
1-2eissn
0301-486Xissn
1573-0832journal_volume
180pub_type
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