Enzyme-linked immunosorbent assay for secalonic acid D.

Abstract:

:A competitive enzyme-linked immunosorbent assay was developed for the xanthone dimer secalonic acid D. The immunogen and enzyme marker were prepared by direct reaction of secalonic acid D with bovine serum albumin and horseradish peroxidase, respectively. The resultant conjugates were characterized by UV/VIS spectra and thin layer chromatography. The hapten:protein ratios in the conjugates were estimated by difference UV/VIS spectra and by fluorescent techniques. Immunization procedures were conducted utilizing New Zealand rabbits over a period of 12 weeks. The competitive enzyme-linked immunosorbent assay on microtiter plates showed that secalonic acid D was detectable within a range of 250-25 000 ng/assay.

journal_name

Mycopathologia

journal_title

Mycopathologia

authors

Neucere JN

doi

10.1007/BF00437013

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

39-43

issue

1

eissn

0301-486X

issn

1573-0832

journal_volume

93

pub_type

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