Abstract:
:A competitive enzyme-linked immunosorbent assay was developed for the xanthone dimer secalonic acid D. The immunogen and enzyme marker were prepared by direct reaction of secalonic acid D with bovine serum albumin and horseradish peroxidase, respectively. The resultant conjugates were characterized by UV/VIS spectra and thin layer chromatography. The hapten:protein ratios in the conjugates were estimated by difference UV/VIS spectra and by fluorescent techniques. Immunization procedures were conducted utilizing New Zealand rabbits over a period of 12 weeks. The competitive enzyme-linked immunosorbent assay on microtiter plates showed that secalonic acid D was detectable within a range of 250-25 000 ng/assay.
journal_name
Mycopathologiajournal_title
Mycopathologiaauthors
Neucere JNdoi
10.1007/BF00437013subject
Has Abstractpub_date
1986-01-01 00:00:00pages
39-43issue
1eissn
0301-486Xissn
1573-0832journal_volume
93pub_type
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