Abstract:
:Candida albicans biofilms on most medical devices are exposed to a flow of body fluids that provide water and nutrients to the fungal cells. While C. albicans biofilms grown in vitro under static conditions have been exhaustively studied, the same is not true for biofilms developed under continuous flow of replenishing nutrients. Here, we describe a simple flow biofilm (FB) model that can be built easily with materials commonly available in most microbiological laboratories. We demonstrate that C. albicans biofilms formed using this flow system show increased architectural complexity compared to biofilms grown under static conditions. C. albicans biofilms under continuous medium flow grow rapidly, and by 8 h show characteristics similar to 24 h statically grown biofilms. Biomass measurements and microscopic observations further revealed that after 24 h of incubation, FB was more than twofold thicker than biofilms grown under static conditions. Microscopic analyses revealed that the surface of these biofilms was extremely compact and wrinkled, unlike the open hyphal layer typically seen in 24 h static biofilms. Results of antifungal drug susceptibility tests showed that C. albicans cells in FB exhibited increased resistance to most clinically used antifungal agents.
journal_name
Mycopathologiajournal_title
Mycopathologiaauthors
Uppuluri P,Chaturvedi AK,Lopez-Ribot JLdoi
10.1007/s11046-009-9205-9subject
Has Abstractpub_date
2009-09-01 00:00:00pages
101-9issue
3eissn
0301-486Xissn
1573-0832journal_volume
168pub_type
杂志文章相关文献
MYCOPATHOLOGIA文献大全abstract:BACKGROUND:A reformulated skin test for coccidioidomycosis, Spherusol®, was recently approved for use in the USA. We hypothesized that it could be useful in predicting severity of illness and outcome in various types of coccidioidomycosis. METHODS:Subjects with non-meningeal coccidioidomycosis attending a clinic in th...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-019-00356-5
更新日期:2019-08-01 00:00:00
abstract::Species of Aspergillus section Flavi were isolated and identified in maize samples collected from a wide geographical region in northeastern China. Aspergillus flavus was identified to be the primary species in the population (99%) becoming a potential source of aflatoxin contamination in the region. Isolated A. flavu...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-007-9029-4
更新日期:2007-08-01 00:00:00
abstract::This study was conducted to evaluate the possible protector effect of bentonite and zeolite in Bovans chicks fed a diet containing 59 mg kg(-1) of fumonisin B1 (FB1) during 3 weeks. A total of 200 one-day-old male chicks were treated varying the amount of bentonite and zeolite. Chick weight was registered weekly. At t...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-012-9534-y
更新日期:2012-09-01 00:00:00
abstract::A dematiaceous fungus, Phialophora richardsiae (Nannf.) Conant, was isolated from human bone. In culture the fungus produced no yeast forms and was less pigmented than two other P. richardsiae isolates. While growth rates were similar, colonial forms differed. Phialides were of two kinds. While both had broad bases an...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00436495
更新日期:1984-05-30 00:00:00
abstract::Talaromyces marneffei, previously known as Penicillium marneffei, is the most important pathogenic thermally dimorphic fungus causing systemic mycosis in Southeast Asia. Traditionally, T. marneffei infection in human was mainly associated with acquired immunodeficiency syndrome caused by HIV infection. In recent years...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-017-0239-0
更新日期:2018-06-01 00:00:00
abstract::The diagnosis of invasive fungal infections (IFI) remains a challenge, particularly for diseases caused by filamentous fungi such as Aspergillus species. Unfortunately, many patients affected by these conditions are not identified before autopsy. Therefore, there is a need for new diagnostic methods for IFI. Galactoma...
journal_title:Mycopathologia
pub_type: 杂志文章,评审
doi:10.1007/s11046-007-9010-2
更新日期:2007-04-01 00:00:00
abstract::A mycotic keratitis case which was caused by Curvularia lunata var. aeria in a patient with an injury in the eye is described. The diagnosis was based on the mycologic analysis of several samples taken from the ulcer of cornea. In vitro tests of the sensitivity of the isolated species to several antifungal drugs were ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00437007
更新日期:1986-01-01 00:00:00
abstract::The number of new fungal pathogens is increasing due to growing population of immunocompromised patients and advanced identification techniques. Fereydounia khargensis is a yeast and was first described in 2014 from environmental samples. As far as we know, this is the first report of human infections associated with ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-016-0002-y
更新日期:2016-08-01 00:00:00
abstract::This is a comparative study to isolate the dermatophytes of tinea capitis using the cytobrush and comparing it versus the standard method. A prospective, observational, comparative trial of 178 probable cases of tinea capitis was conducted in two dermatological centers. Each patient underwent mycological tests that in...
journal_title:Mycopathologia
pub_type: 临床试验,杂志文章
doi:10.1007/s11046-007-9019-6
更新日期:2007-06-01 00:00:00
abstract::Isolation frequency of Ophiosphaerella korrae (spring dead spot pathogen) from Cynodon dactylon (bermudagrass) roots at a golf course near West Point, Mississippi, was monitored over a 3-year investigation. Laboratory and greenhouse experiments were conducted to determine optimal temperatures for the growth of selecte...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-010-9273-x
更新日期:2010-05-01 00:00:00
abstract::Two hundred strains of the Aspergillus flavus group isolated from groundnuts (peanuts) growing in Israel were examined for their ability to produce mycotoxins in potato dextrose (PD) broth. Almost 77% of the isolates produced aflatoxin; aflatoxins B1 and B2 were formed by most of the isolates. Simultaneous production ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF01103479
更新日期:1993-06-01 00:00:00
abstract::Fusarium acuminatum NRRL 6227 produces an antifungal metabolite that causes incubating conidia of several Penicillium species to swell 5-10 diameters while inhibiting germination. The swollen conidia are spherical, translucent, nonviable and easily shattered with a slight physical pressure; however, they remain resist...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00491996
更新日期:1977-11-30 00:00:00
abstract::Sunflower (Helianthus annuus) stem canker caused by Diaporthe helianthi is one of the most important sunflower diseases in Croatia. Until recently, sunflower was the only known host for D. helianthi. In our research carried out in the area of Eastern Croatia, isolates of Diaporthe/Phomospis were collected from Xanthiu...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-010-9289-2
更新日期:2010-07-01 00:00:00
abstract::Adult CBA mice showed considerably greater resistance to experimental infection with Aspergillus fumigatus than young animals of the same strain (P less than 0.01). Furthermore, in contrast with the young mice, the susceptibility of the adults was only moderately increased by treatment with cortico-steroids. On the ot...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00490376
更新日期:1977-02-18 00:00:00
abstract::An intracellular beta-hemolysin capable of lysing human, sheep and cow erythrocytes but not cells from some other animals was isolated from the cell walls of the three developmental cell-forms of Nocardia asteroides and characterised. The spherical cell-forms contained the highest amounts of the hemolysin (100 h.u./mg...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00436319
更新日期:1986-07-01 00:00:00
abstract::Using the grain-plate method and on glucose- Czapek 's agar at 28 degrees C, fifty-eight species belonging to 26 genera were collected from barley (42 species and 19 genera), maize (29 species and 16 genera), sorghum (32 species and 17 genera) and wheat grains (42 species and 18 genera). The most frequent genera were ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00436702
更新日期:1984-03-15 00:00:00
abstract::Soils from 100 irrigated fields (95 under vegetables, 5 under citrus) in different geographical locations in the West Bank (Palestinian Autonomous Territory) were surveyed for hymexazol-insensitive (HIS) Pythium species using the surface soil dilution plate (SSDP) method with the VP3 medium amended with 50 mg/L hymexa...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1023/b:myco.0000003561.47451.f7
更新日期:2003-01-01 00:00:00
abstract::The taxonomic position of the causal agent of fruit rot of olives was determined from fresh collections of the fungus from central Greece. In culture it formed two types of conidia, namely fusiform, hyaline, aseptate conidia typical of the genus Fusicoccum, and dark-walled, ovoid, ellipsoid or fusiform, 1-2 septate co...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-005-0256-2
更新日期:2005-04-01 00:00:00
abstract::Scopoletin (7-hydroxy-6-methoxy coumarin) which inhibited the conidial germination of Corynespora cassiicola was isolated from the uninfected mature leaves of Hevea brasiliensis. Scopoletin was not detected in uninfected immature rubber leaves. The immature leaves produced scopoletin after being infected with C. cassi...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1023/a:1014910132595
更新日期:2002-01-01 00:00:00
abstract::We describe a case of tinea unguis in a 20 year old female student from Caracas. The peri-ungual skin of a thumb appeared altered first and, since 6 months, the lamina was being progressively invaded and destroyed. At the direct mycologic examination numerous, regular and irregular, hyaline fungal hyphae were visible....
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00436983
更新日期:1983-02-17 00:00:00
abstract::Four cases of Tinea Nigra by Exophiala werneckii, observed in Porto Alegre, RS, during the period 1981-1992 were related. A boy presented bilateral palmar lesions, one girl had plantar lesions and the remaining two girls had lesions on the palms. Three cases were autochthonous and the remaining patient was infected du...
journal_title:Mycopathologia
pub_type: 杂志文章,评审
doi:10.1007/BF01103769
更新日期:1994-06-01 00:00:00
abstract::The present study reports the histopathological findings of 86 skin lesions of dogs with sporotrichosis from Rio de Janeiro. Suppurative granulomatous inflammation was the predominant finding and was observed in 76 (88.37%) cases. Plasma cells surrounding the suppurative granulomas were detected in 68 (89.5%) cases an...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-009-9198-4
更新日期:2009-08-01 00:00:00
abstract::Candidiasis was experimentally produced in young Japanese quail by oral administration of Candida albicans cells. Lesions were confined to upper digestive tract with most characteristic changes occurring on the mucosa of crop. No lesions were observed in other tissues of the body. The initial changes in the crop were ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF01103575
更新日期:1993-02-01 00:00:00
abstract::In vivo interactions of rabbit alveolar macrophages (AM) and Cryptococcus neoformans, a yeast pathogenic for humans, were studied. As a control, inert silica particles of a similar diameter (5-6 microns) were used. Of 16 rabbits, 6 were instilled intratracheally with fluorescein-labelled heat-killed C. neoformans, 6 w...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1023/a:1006843202124
更新日期:1997-01-01 00:00:00
abstract::A case of a 62-year-old man with Candida krusei peritonitis secondary to duodenal perforation due to Candida duodenitis that was successfully treated with a 14-day course of caspofungin is reported. The potential role of Candida infection in the pathogenesis of peptic ulcers and duodenal perforation is considered. If ...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-010-9344-z
更新日期:2011-01-01 00:00:00
abstract::Chronic exposure to moniliformin results in the development of myocardial hypertrophy and degeneration. The cause of this hypertrophy is unknown. However, moniliformin-induced hypoxia or altered function of cardiac pyruvate dehydrogenase, rather than direct cardiostimulation have been proposed as potential mechanisms....
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00439122
更新日期:1996-01-01 00:00:00
abstract::The antifungal activity of base-soluble proteins (BSP) and methanol-soluble polysaccharides (PS) from A. flavus resistant (Yellow Creole) and susceptible (Huffman) genotypes of corn were investigated by in vitro studies. Bioassays of fungal growth inhibition in agar media showed antifungal activity by proteins and pol...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/BF00442418
更新日期:1991-02-01 00:00:00
abstract::Nocardia beijingensis, a recently established new species, is an isolate from soil in China. During our taxonomic studies on 450 nocardial clinical isolates in Thailand and Japan, 17 strains from Thailand and 1 strain from Japan were found to have a similar physiological characteristic to those of N. beijingensis, suc...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1023/b:myco.0000020588.60081.37
更新日期:2004-02-01 00:00:00
abstract::Scedosporium and Lomentospora species are the second most frequent colonizing, allergenic, or invasive fungal pathogens in patients with cystic fibrosis, and are responsible for infections varying from cutaneous and subcutaneous tissue infections caused by traumatic inoculation to severe systemic diseases in immunocom...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-017-0195-8
更新日期:2017-12-01 00:00:00
abstract::Invasive aspergillosis can be difficult to diagnose, and early recognition and initiation of therapy is imperative for improving patient outcomes. A case of invasive Aspergillus laryngotracheobronchitis is presented here with a review of the relevant literature. A 58-year-old male undergoing treatment for CNS lymphoma...
journal_title:Mycopathologia
pub_type: 杂志文章
doi:10.1007/s11046-017-0123-y
更新日期:2017-08-01 00:00:00