Involvement of small GTP-binding proteins in defense signal-transduction pathways of higher plants.

abstract：:In vitro translation of cellular poly(A)+ RNA coupled with immunoprecipitation was developed as a technique for characterizing 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] receptors and assessing receptor mRNA activity. Cell-free translation of poly(A)+ RNA isolated from chicken intestine revealed two immunoprecipitable fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mangelsdorf DJ,Pike JW,Haussler MR

更新日期：1987-01-01 00:00:00

abstract：:The DNA origami method permits folding of long single-stranded DNA into complex 3D structures with subnanometer precision. Transmission electron microscopy, atomic force microscopy, and recently cryo-EM tomography have been used to characterize the properties of such DNA origami objects, however their microscopic stru...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoo J,Aksimentiev A

更新日期：2013-12-10 00:00:00

abstract：:The murine T-cell surface molecules Lyt-2 and L3T4 play a role in the activation of antigen-specific T cells. The currently accepted model for the function of these molecules proposes that Lyt-2 and L3T4 increase the overall avidity of the interaction between the T-cell antigen receptor and antigen in association with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fazekas de St Groth B,Gallagher PF,Miller JF

更新日期：1986-04-01 00:00:00

abstract：:Figs (Ficus spp., Moraceae) and their pollinating wasps (Agaonidae, Chalcidoidea) constitute perhaps the most tightly integrated pollination mutualism that is known. Figs are characterized by extraordinarily high global and local species diversity. It has been proposed that the diversification of this mutualism has oc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Machado CA,Robbins N,Gilbert MT,Herre EA

更新日期：2005-05-03 00:00:00

abstract：:Molecular junctions are essentially modified electrodes familiar to electrochemists where the electrolyte is replaced by a conducting "contact." It is generally hypothesized that changing molecular structure will alter system energy levels leading to a change in the transport barrier. Here, we show the conductance of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sayed SY,Fereiro JA,Yan H,McCreery RL,Bergren AJ

更新日期：2012-07-17 00:00:00

abstract：:The P23T mutant of human gammaD-crystallin (HGD) is associated with cataract. We have previously investigated the solution properties of this mutant, as well as those of the closely related P23V and P23S mutants, and shown that although mutations at site 23 of HGD do not produce a significant structural change in the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McManus JJ,Lomakin A,Ogun O,Pande A,Basan M,Pande J,Benedek GB

更新日期：2007-10-23 00:00:00

abstract：:Sensory neurons perceive environmental cues and are important of organismal survival. Peripheral sensory neurons interact intimately with glial cells. While the function of axonal ensheathment by glia is well studied, less is known about the functional significance of glial interaction with the somatodendritic compart...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yadav S,Younger SH,Zhang L,Thompson-Peer KL,Li T,Jan LY,Jan YN

更新日期：2019-03-12 00:00:00

abstract：:The observed intensities of the reflections from the body-centered cubic phase of lipid systems are shown to be incompatible with a recently reported model consisting of straight, indefinitely long rods. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luzzati V,Tardieu A,Gulik-Krzywicki T

更新日期：1981-08-01 00:00:00

abstract：:Aotus monkeys, which are generally killed when infected with the human malaria parasite Plasmodium falciparum, have been identified and grouped by karyotype. These animals were immunized with parasite material obtained from P. falciparum cultivated in vitro which had been maintained in culture for over a year. When su...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reese RT,Trager W,Jensen JB,Miller DA,Tantravahi R

更新日期：1978-11-01 00:00:00

abstract：:The polypeptide precursor pr76 to the internal viral group specific (gs) antigen proteins of Rous sarcoma virus, synthesized in a cell-free system of ascites cells, has been processed in vitro into the viral proteins by purified viral protein p15 as well as by disrupted Rous sarcoma virus. Disrupted Rauscher murine le...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： von der Helm K

更新日期：1977-03-01 00:00:00

abstract：:Bitemporal injections of puromycin that primarily affect the hippocampal-entorhinal area induce amnesia of aversive maze-learning in mice for 3 days after training but are ineffective 6 or more days after training. At these later times, additional puromycin sites covering widespread forebrain areas are necessary to in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flexner JB,Flexner LB,Church AC,Rainbow TC,Brunswick DJ

更新日期：1985-11-01 00:00:00

abstract：:When purified dimers of hemagglutinin-neuraminidase molecules released by protease digestion from three strains of human parainfluenza virus 1 were used in crystallization trials, long thin needle crystals formed. Electron microscopic analysis of these needle crystals revealed that they are composed of stacks of tripl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murti KG,Takimoto T,Laver WG,Portner A

更新日期：1993-02-15 00:00:00

abstract：:The 24 human chromosome types of normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophoto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrano AV,Gray JW,Langlois RG,Burkhart-Schultz KJ,Van Dilla MA

更新日期：1979-03-01 00:00:00

abstract：:In a study of juvenile hormone-regulated gene expression, we isolated an anonymous cDNA representing a message that was strongly suppressed by juvenile hormone in the fat body of the cockroach Blaberus discoidalis. The protein deduced from the cDNA sequence showed compelling resemblance in sequence to the transferrins...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamroz RC,Gasdaska JR,Bradfield JY,Law JH

更新日期：1993-02-15 00:00:00

abstract：:Tunas are migratory fishes in offshore habitats and top predators with unique features. Despite their ecological importance and high market values, the open-ocean lifestyle of tuna, in which effective sensing systems such as color vision are required for capture of prey, has been poorly understood. To elucidate the ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura Y,Mori K,Saitoh K,Oshima K,Mekuchi M,Sugaya T,Shigenobu Y,Ojima N,Muta S,Fujiwara A,Yasuike M,Oohara I,Hirakawa H,Chowdhury VS,Kobayashi T,Nakajima K,Sano M,Wada T,Tashiro K,Ikeo K,Hattori M,Kuhara S,

更新日期：2013-07-02 00:00:00

abstract：:H-2(q) mice (DBA/1) do not make an antibody response to a synthetic aminoacid polymer, (Glu, Ala,Tyr(10)), after an immunizing regimen that produces a good antibody response in mouse strains with other H-2 alleles. Their thymocytes, however, show evidence of recognizing this antigen since they synthesize DNA when they...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gershon RK,Maurer PH,Merryman CF

更新日期：1973-01-01 00:00:00

abstract：:Gyrate atrophy of the choroid and retina (GA) is an inherited chorioretinal degeneration caused by deficiency of ornithine delta-aminotransferase (OAT; L-ornithine: 2-oxo-acid aminotransferase; EC 2.6.1.13). GA is one of the "Finnish genetic diseases," a group of several rare monogenic disorders that occur with increa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell GA,Brody LC,Sipila I,Looney JE,Wong C,Engelhardt JF,Patel AS,Steel G,Obie C,Kaiser-Kupfer M

更新日期：1989-01-01 00:00:00

abstract：:To explore the basis for the template specificities of the bacteriophage T3 and T7 RNA polymerases (EC 2.7.7.6), we determined the nucleotide sequences of six promoters recognized by the T3 RNA polymerase and compared them with the previously determined promoter sequences recognized by the bacteriophage T7 RNA polymer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bailey JN,Klement JF,McAllister WT

更新日期：1983-05-01 00:00:00

abstract：:Accumulation of amyloid-beta (Aβ), which is associated with Alzheimer's disease, can be caused by excess production or insufficient clearance. Because of its β-sheet structure, fibrillar Aβ is resistant to proteolysis, which would contribute to slow degradation of Aβ plaques in vivo. Fibrillar Aβ can be internalized b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Solé-Domènech S,Rojas AV,Maisuradze GG,Scheraga HA,Lobel P,Maxfield FR

更新日期：2018-02-13 00:00:00

abstract：:Aquaporin (AQP) 4 is the predominant water channel in the mammalian brain, abundantly expressed in the blood-brain and brain-cerebrospinal fluid interfaces of glial cells. Its function in cerebral water balance has implications in neuropathological disorders, including brain edema, stroke, and head injuries. The 1.8-A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho JD,Yeh R,Sandstrom A,Chorny I,Harries WE,Robbins RA,Miercke LJ,Stroud RM

更新日期：2009-05-05 00:00:00

abstract：:A recently described experimental system for analyzing the mode of action of a morphogen gradient involves the in situ hybridization of sectioned tissue constructs. In these constructs, a source of activin signaling induces the transcription of several mesodermal genes in blastula animal caps, according to the positio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gurdon JB,Mitchell A,Ryan K

更新日期：1996-09-03 00:00:00

abstract：:Escherichia coli lipopolysaccharide (LPS)-induced nitrate biosynthesis was studied in LPS-sensitive C3H/He and LPS-resistant C3H/HeJ mice. Intraperitoneal injection of 15 micrograms of LPS led to a temporary 5- to 6-fold increase in blood nitrate concentration in the C3H/He strain. Levels of nitrate excreted in the ur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stuehr DJ,Marletta MA

更新日期：1985-11-01 00:00:00

abstract：:Adenosine has been identified in the anterior pituitary gland and is secreted from cultured folliculostellate (FS) cells. To determine whether adenosine controls the secretion of anterior pituitary hormones in vitro, adenosine was incubated with anterior pituitaries. It stimulated prolactin (PRL) release at the lowest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu WH,Kimura M,Walczewska A,Porter JC,McCann SM

更新日期：1998-06-23 00:00:00

abstract：:Whether electromagnetic precursors to earthquakes (EQs) exist is an important question not only for EQ prediction but also for understanding the physical processes of EQ generation. Slow transient geoelectric potential changes have been observed before several recent EQs in Japan. In most cases, they appeared 1-19 day...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Uyeda S,Nagao T,Orihara Y,Yamaguchi T,Takahashi I

更新日期：2000-04-25 00:00:00

abstract：:Human thioredoxin reductase (TR) contains selenocysteine (Secys) in a redox center [cysteine (Cys)-497,Secys-498] near the C-terminus. The essential role of Secys in TR isolated from HeLa cells was demonstrated by the alkylation studies. Reaction of native NADPH reduced enzyme with bromoacetate at pH 6.5 inhibited enz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gorlatov SN,Stadtman TC

更新日期：1998-07-21 00:00:00

abstract：:The phylogenetic affinities of the grass family (Poaceae) have long been debated. The chloroplast genomes of at least some grasses have been known to possess three inversions relative to the typical gene arrangement found in most flowering plants. We have surveyed for the presence of these inversions in grasses and ot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doyle JJ,Davis JI,Soreng RJ,Garvin D,Anderson MJ

更新日期：1992-08-15 00:00:00

abstract：:The electrical response of a pigmented lipid bilayer to a short laser pulse is measured by a tunable voltage clamp method. In this method, a variable access impedance permits "tuning" of the observed relaxations for optimal measurements. Analysis of the data so obtained leads to an equivalent circuit that contains a n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong FT,Mauzerall D

更新日期：1974-04-01 00:00:00

abstract：:Changes in nuclear K+ electrochemical activity and total nuclear K+ content in salivary glands of Chironomus tentans were measured with ion-selective microelectrodes based on valinomycin and with flameless atomic absorption spectrometry, respectively. The K+ activity increased by a factor of 2.6 and the total K+, by a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wuhrmann P,Ineichen H,Riesen-Willi U,Lezzi M

更新日期：1979-02-01 00:00:00

abstract：:A monomorphic anti-HLA-G monoclonal antibody (mAb) was obtained by immunization of HLA-B27/human beta 2-microglobulin double-transgenic mice with transfected murine L cells expressing both HLA-G and human beta 2-microglobulin. This mAb, designated BFL.1, specifically recognizes, by flow cytometry analysis, the immuniz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bensussan A,Mansur IG,Mallet V,Rodriguez AM,Girr M,Weiss EH,Brem G,Boumsell L,Gluckman E,Dausset J

更新日期：1995-10-24 00:00:00

abstract：:Particles composed of secondary organic material (SOM) are abundant in the lower troposphere. The viscosity of these particles is a fundamental property that is presently poorly quantified yet required for accurate modeling of their formation, growth, evaporation, and environmental impacts. Using two unique techniques...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renbaum-Wolff L,Grayson JW,Bateman AP,Kuwata M,Sellier M,Murray BJ,Shilling JE,Martin ST,Bertram AK

更新日期：2013-05-14 00:00:00