Abstract：
OBJECTIVE:To identify transcripts whose expression is restricted to germ cells. DESIGN:Expressed sequence tags (ESTs) from unfertilized egg libraries were utilized to perform in silico subtraction and identify germ cell-specific transcripts. SETTING:Baylor College of Medicine, Houston, Texas. ANIMAL(S):C57BL/6J/129SvEv hybrid. INTERVENTION(S):Tissue harvesting from mice. MAIN OUTCOME MEASURE(S):Identification of germ cell-specific transcripts. RESULT(S):We have used the Unigene collection of mouse cDNA libraries to identify ESTs derived from unfertilized egg libraries. A total of 3,499 ESTs were identified from Knowles Solter and Ko unfertilized egg cDNA libraries. In silico subtraction identified 258 ESTs, which were found in these unfertilized egg libraries, but not in adult mouse tissue cDNA libraries. We performed reverse transcription polymerase chain reaction (RT-PCR) on multiple adult tissues with 43 selected ESTs and found 5 of them where expression was absent in heart, lung, liver, brain, spleen, stomach, intestines, kidneys, and uterus, but restricted to ovaries and testes. Three ESTs were further analyzed, and they were exclusively localized to the oocytes by in situ hybridization. CONCLUSION:We have shown that utilization of publicly available ESTs from murine EST libraries is a simple and rapid in silico approach to the identification of transcripts preferentially expressed in germ cells.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Rajkovic A,Yan M S C,Klysik M,Matzuk Mdoi
10.1016/s0015-0282(01)01966-5keywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
550-4issue
3eissn
0015-0282issn
1556-5653pii
S0015-0282(01)01966-5journal_volume
76pub_type
杂志文章abstract：:A total of 82 patients underwent 106 cycles in which immature oocytes were recovered after hCG priming and transferred at blastocyst-stage. After transfer, the implantation rate was 26.8%, and the clinical pregnancy rate was 51.9%. We found that the oocytes retrieved after hCG priming in women with high risk of ovaria...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.11.027
更新日期：2007-06-01 00:00:00
abstract：OBJECTIVE:A practical attempt to simplify and improve the technique of artificial uterine cavity distension by instilling gel instead of flushing saline. DESIGN:Prospective observational study. SETTING:Teaching hospital. PATIENT(S):One hundred twenty patients with an indication for sonohysterography (abnormal uterin...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.05.075
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVE:To discuss the new peer review system at the National Institutes of Health (NIH) and to present the process undertaken to accomplish these changes, with an emphasis on the reproductive sciences. DESIGN:A discussion of the many NIH committees and their composition and reports leading to the changes in peer re...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.10.006
更新日期：2004-02-01 00:00:00
abstract：:Effective contraception and easily available abortion have contributed to the decline of adoptable children. This decline has resulted in a marked increase in the demand for AID. The provider of donor semen must establish minimal semen criteria to meet this demand effectively. During the past 8 years the authors have ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)45923-6
更新日期：1981-12-01 00:00:00
abstract：OBJECTIVE:Our objective is to report observed changes in thyroid-stimulating hormone (TSH) in two patients undergoing super-ovulation for IVF. DESIGN:Case report. SETTING:Private assisted reproduction practices. PATIENT(S):Two hypothyroid women taking thyroxine replacement therapy undergoing super-ovulation for IVF....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.11.051
更新日期：2010-05-01 00:00:00
abstract：:The purpose of this study was to investigate the prognosis for fertility in children with hydrocele. Our results show that hydrocele seems to be more of a symptom than an actual pathological entity. It occurs both with an open and a closed processus vaginalis. Bilateral hydrocele seems to be a rare occurrence, mostly ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53467-0
更新日期：1990-04-01 00:00:00
abstract：OBJECTIVE:To compare pathologic characteristics and epidemiologic risk factors for uterine fibroids in African American and white women undergoing hysterectomy. DESIGN:Cross-sectional analysis of women undergoing premenopausal hysterectomy. SETTING:Two university-associated hospitals in North Carolina. PATIENT(S):Af...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.10.039
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:To determine intrafollicular hormone levels and characterize the mRNA expression of the insulin-like growth factor (IGF) receptors, IGF binding proteins (IGFBP), and pregnancy-associated plasma protein-A (PAPP-A) in granulosa cells before and after an ovulatory hCG stimulus. DESIGN:Experimental animal study....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.12.096
更新日期：2010-03-15 00:00:00
abstract：OBJECTIVE:To determine whether endometrial hCG infusion at the time of human blastocyst transfer impacts implantation rates. DESIGN:Randomized double-blinded placebo-controlled trial. SETTING:Academic. PATIENT(S):Infertile couples with the female partner less than 43 years old (n = 300) undergoing fresh or frozen ET...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2014.08.006
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVE:To improve the maturation rate of murine and human germinal vesicle (GV) oocytes using human tubal epithelial cells (hTECs). DESIGN:Murine and human GV oocytes were randomized to human tubal fluid (HTF) media alone or cocultured with mouse embryonic fibroblasts (MEFs) or primary hTECs or immortalized hTECS (...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.08.034
更新日期：2011-03-15 00:00:00
abstract：OBJECTIVE:To evaluate how varicocele repair (VR) impacts pregnancy (PRs) and live birth rates in infertile couples undergoing assisted reproduction wherein the male partner has oligospermia or azoospermia and a history of varicocele. DESIGN:Systematic review and meta-analysis. SETTING:Not applicable. PATIENT(S):Azoo...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.fertnstert.2016.07.1093
更新日期：2016-11-01 00:00:00
abstract：:Paternal aging is associated with increased risk of genetic disease transmission to the offspring. The changes associated with aging arise predominantly through formation of single nucleotide variation through DNA replication errors, as well as possibly chronic exposure to environmental toxins and reactive oxygen spec...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2016.12.021
更新日期：2017-02-01 00:00:00
abstract：OBJECTIVE:To describe the association of Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome and thrombocytopenia-absent radius (TAR) syndrome. DESIGN:Descriptive case report. SETTING:Tertiary university-affiliated care and referral facility. PATIENT(S):A 22-year-old woman with TAR syndrome referred to our department wit...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.06.077
更新日期：2005-02-01 00:00:00
abstract：OBJECTIVE:To investigate the relationship between sperm chromatin structure assay (SCSA) parameters, DNA fragmentation index (DFI) and high DNA stainability (HDS), and outcomes of IVF and intracytoplasmic sperm injection (ICSI). DESIGN:Retrospective review and prospective study. SETTING:Academic human reproduction la...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2007.06.018
更新日期：2008-08-01 00:00:00
abstract：OBJECTIVE:To present two cases of ectopic twin pregnancy in a Cesarean section scar following IVF-embryo transfer. DESIGN:Case report. SETTING:Tertiary referral case center. PATIENT(S):Two women who had ectopic twin pregnancy in a Cesarean section scar, in both cases following IVF and embryo transfer. INTERVENTION(...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.11.091
更新日期：2008-11-01 00:00:00
abstract：:The specific radioimmunoassay system was developed for the titration of the antibodies to porcine zona pellucida (ZP) in human sera by using 125I-labeled purified porcine ZP as antigen, which is known to have cross-reactivity with human ZP. The antibodies in human sera were detected in 3 of 11 (27%) women with unexpla...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47602-8
更新日期：1984-02-01 00:00:00
abstract：OBJECTIVE:To determine the difference between two laparoscopic methods for the management of endometriomas with regard to recurrence of signs and symptoms and pregnancy rate. DESIGN:Prospective, randomized clinical trial. SETTING:Infertility and gynecologic endoscopy units of two medical university hospitals. PATIEN...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/j.fertnstert.2004.04.067
更新日期：2004-12-01 00:00:00
abstract：OBJECTIVE:To determine the contribution of several variables to fluid loss during transcervical resection of submucous myomas. DESIGN:An observational study using multiple linear regression analyses. SETTING:A university-affiliated training hospital and a university department of clinical epidemiology and biostatisti...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00335-x
更新日期：1997-11-01 00:00:00
abstract：OBJECTIVE:To assess whether seminal vesicle sperm aspiration (SVSA) is an option for wounded warriors with severe genital and testicular injuries, with the goal of cryopreservation to use in future assisted reproductive technology (ART) cycles. DESIGN:Retrospective case series. SETTING:Tertiary care military hospital...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.05.028
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVE:To evaluate spontaneous apoptosis in single-cell suspensions of eutopic and ectopic endometrium from women with endometriosis and in eutopic endometrium from fertile controls without endometriosis. DESIGN:Paired specimens of eutopic and ectopic endometrial tissue from patients with endometriosis and eutopic ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00073-9
更新日期：1998-06-01 00:00:00
abstract：:Turner syndrome (TS), which affects approximately 1 in 2,500 live-born females, is characterized by loss or structural anomalies of an X chromosome. Clinical features vary among patients; multiple organ systems can be affected. Endocrinologists are involved in the management of short stature, delayed puberty, and infe...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2012.07.1104
更新日期：2012-10-01 00:00:00
abstract：OBJECTIVE:To quantify the effect of the new 2010 World Health Organization (WHO) semen analysis reference values on reclassifying previous semen analysis parameters and definition of patients with male factor infertility. DESIGN:A multi-institutional retrospective chart review. SETTING:University and private male inf...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.07.1130
更新日期：2012-12-01 00:00:00
abstract：:Tamoxifen therapy in premenopausal women with breast cancer may be associated with the development of ovarian cysts. In this report, a case of ovarian torsion associated with TAM therapy is described. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55688-x
更新日期：1993-02-01 00:00:00
abstract：:Twelve human uteri containing intrauterine contraceptive devices (IUDs) and ten uteri without IUDs were obtained at hysterectomy. Samples of fluid were collected from the uterine lumina by absorbing the fluid in small pieces of lens paper. In the samples of luminal fluid we measured the concentration of beta-galactosi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42078-9
更新日期：1976-09-01 00:00:00
abstract：OBJECTIVE:To describe trends in age-specific incidence rates of female sterilization (FS) procedures in Western Australia and to evaluate the effects of the introduction of government-subsidized contraceptive methods and the implementation of the Australian government's baby bonus policy on FS rates. DESIGN:Population...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.04.035
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy of transvaginal intratubal methotrexate (MTX) treatment of tubal ectopic pregnancy (EP). SETTING:Outpatient setting in University Hospital. PATIENTS:Forty women with early EP and rising serum beta-human chorionic gonadotropin (beta-hCG) levels. INTERVENTION:Transvaginal intratubal ...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)55143-7
更新日期：1992-07-01 00:00:00
abstract：OBJECTIVE:To report a case of parasitic leiomyoma of the abdominal wall after laparoscopic myomectomy. DESIGN:Case report. SETTING:Large medical center. PATIENT(S):A 31-year-old woman with a newly developed palpable mass in the abdominal wall near the trocar site of a previous laparoscopic myomectomy. INTERVENTION(...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.08.068
更新日期：2008-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the expression of type IV collagen [alpha1(IV) to alpha6(IV)] in testes and the association with spermatogenesis. DESIGN:Retrospective immunohistochemical study. SETTING:Division of Urology, Department of Organs Therapeutics, Faculty of Medicine, in a university hospital. PATIENT(S):Testicular ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(03)00775-1
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:To compare the incidence of noncompliance measured objectively by a home use fertility monitor with the traditional self-reported incidence of compliance in a study of a new method of contraception. DESIGN:Prospective cohort study. SETTING:A large family planning clinic in Edinburgh. PATIENT(S):Thirty-two ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02899-0
更新日期：2001-12-01 00:00:00
abstract：:Solid-phase enzyme immunoassays, based on monoclonal antibodies reactive with different forms of human placental-type alkaline phosphatases, were applied to their detection in human seminal plasma from fertile and infertile men. The placental-like form of alkaline phosphatase (PLAP-like AP), known to occur in testicul...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
