Abstract:
:At mammalian neuromuscular junctions (NMJs), innervation induces and maintains the metabolic stability of acetylcholine receptors (AChRs). To explore whether neural agrin may cause similar receptor stabilization, we injected neural agrin cDNA of increasing transfection efficiencies into denervated adult rat soleus (SOL) muscles. As the efficiency increased, the amount of recombinant neural agrin expressed in the muscles also increased. This agrin aggregated AChRs on muscle fibers, whose half-life increased in a dose-dependent way from 1 to 10 days. Electrical muscle stimulation enhanced the stability of AChRs with short half-lives. Therefore, neural agrin can stabilize aggregated AChRs in a concentration- and activity-dependent way. However, there was no effect of stimulation on AChRs with a long half-life (10 days). Thus, at sufficiently high concentrations, neural agrin alone can stabilize AChRs to levels characteristic of innervated NMJs.
journal_name
Proc Natl Acad Sci U S Aauthors
Bezakova G,Rabben I,Sefland I,Fumagalli G,Lømo Tdoi
10.1073/pnas.171539698keywords:
subject
Has Abstractpub_date
2001-08-14 00:00:00pages
9924-9issue
17eissn
0027-8424issn
1091-6490pii
171539698journal_volume
98pub_type
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