Evaluation of human sperm membrane integrity using the water test and the hypoosmotic test.

Abstract:

:The objective of the study was to compare the water test and the hypoosmotic test (HOS) in the assessment of the human sperm membrane. A total of 686 semen samples from human male donors were subjected to water and HOS tests after routine semen evaluation. The mean percentage of swollen spermatozoa was 71.8 +/- 9.6% in the HOS test and 67.8 +/- 9.4% for the water test; these values were not statistically different. The correlation of coefficients between the water test and the HOS test was highly significant whether the values for the HOS test were higher or lower than 60% (P < 0.001). A poor correlation was obtained when the two tests were compared for sperm counts either higher or lower than 20 x 6 ml-1 and when the results for both tests were compared with the percentage of eosin-Y staining spermatozoa. A poor correlation was also obtained when the results of each test were compared with eosin-Y staining spermatozoa in normal and abnormal semen samples. The coefficient of regression between the two tests showed a high correlation (P < 0.001). In conclusion, even though a high correlation between the HOS test and water test was observed in this study, it is not possible to recommend assessment of sperm membrane integrity using the water test and the consequent replacement of the HOS test in routine practice. Further studies are necessary to establish the best test for sperm vitality.

journal_name

Andrologia

journal_title

Andrologia

authors

Bahamondes L,Fazano F,De Lucio MA,Neves PA,Bottcher Luiz F,Lorenzetti GB

doi

10.1046/j.1439-0272.2001.00374.x

keywords:

subject

Has Abstract

pub_date

2001-03-01 00:00:00

pages

75-7

issue

2

eissn

0303-4569

issn

1439-0272

pii

and374

journal_volume

33

pub_type

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