Personal experience with the application of carbohydrate-deficient transferrin (CDT) assays to the detection of congenital disorders of glycosylation.

abstract：:Background Systemic sclerosis (SSc) and primary biliary cholangitis (PBC) are autoimmune diseases that may occur concomitantly and are both strongly associated with disease-specific autoantibodies. This study investigated the prevalence and fine specificity of PBC-specific serology (PBC-Ab) and associations with the S...

journal_title：Clinical chemistry and laboratory medicine

authors： Florin L,Rubben K,Vanhaecke A,Devreese K,De Keyser F,Smith V,Bonroy C

更新日期：2020-02-25 00:00:00

abstract：BACKGROUND:Growth differentiation factor-15 (GDF-15), a stress responsive cytokine, is a promising biomarker of renal functional decline in diabetic kidney disease (DKD). This study aimed primarily to establish normative data and secondarily to evaluate the potential utility of GDF-15 in DKD using Roche Diagnostics ele...

journal_title：Clinical chemistry and laboratory medicine

authors： Hamon SM,Griffin TP,Islam MN,Wall D,Griffin MD,O'Shea PM

更新日期：2019-03-26 00:00:00

abstract：BACKGROUND:Our laboratory was confronted with two successive urine samples from a single patient which tested positive for human chorionic gonadotropin (hCG) when tested with both qualitative and quantitative assays, combined with no detectable hCG in corresponding plasma samples. METHODS:Serial dilution and recovery ...

journal_title：Clinical chemistry and laboratory medicine

authors： Albersen A,Kemper-Proper E,Thelen MH,Kianmanesh Rad NA,Hoedemaeker RF,Boesten LS

更新日期：2011-06-01 00:00:00

abstract：:We discovered a hitherto undescribed insertion/deletion (I/D) polymorphism of 7 base pairs in intron 4 of the HP1 allele and intron 4 and 6 of the HP2 allele. Genotyping was performed in 311 Belgian subjects. The association between serum haptoglobin (Hp) and lipid concentrations and the Hp I/D genotypes was investiga...

journal_title：Clinical chemistry and laboratory medicine

authors： Wuyts B,Hetet G,Grandchamp B,Delanghe JR

更新日期：2002-05-01 00:00:00

abstract：:Since PSA is supposed to play an active role in the progression of prostate cancer, we applied a quantitative RT-PCR to measure the absolute levels of prostate-specific antigen (PSA) mRNA expression in benign and malignant prostatic tissue. Consecutive fine needle prostate biopsy material from 59 patients (43 with pro...

journal_title：Clinical chemistry and laboratory medicine

authors： Gelmini S,Tricarico C,Petrone L,Forti G,Amorosi A,Dedola GL,Serio M,Pazzagli M,Orlando C

更新日期：2003-03-01 00:00:00

abstract：:The variability of the tumor markers cancer antigen (CA) 15.3, carcinoembryonic antigen (CEA) and tissue polypeptide antigen (TPA) during steady state concentrations and the rate of increase during progression is described. One hundred and ninety-two patients were monitored during first-line chemotherapy for metastati...

journal_title：Clinical chemistry and laboratory medicine

authors： Sölétormos G,Petersen PH,Dombernowsky P

更新日期：2000-05-01 00:00:00

abstract：:Given the lack of information about Y haplotypes for Campania (southern Italy), we analyzed eight Y short tandem repeats in a sample of males from this region with the aim of establishing a Y-haplotype database that can be used for forensic purposes. The eight Y short tandem repeats were amplified by two PCR multiplex...

journal_title：Clinical chemistry and laboratory medicine

authors： Calcagno G,Labruna G,Sacchetti L

更新日期：2005-01-01 00:00:00

abstract：:Undiagnosed thyroid disease is a common problem with significant public health implications. This is especially true during pregnancy, when the health of both the mother and the developing child can be adversely affected by abnormal maternal thyroid function. Measurement of serum thyroid stimulating hormone (TSH) and ...

journal_title：Clinical chemistry and laboratory medicine

authors： Quinn FA,Gridasov GN,Vdovenko SA,Krasnova NA,Vodopianova NV,Epiphanova MA,Schulten M

更新日期：2005-01-01 00:00:00

abstract：:There is evidence that angiogenesis plays an important role in the progression of multiple myeloma (MM). Hepatocyte growth factor (HGF) and tumor necrosis factor-alpha (TNF-alpha) are cytokines that potently stimulate angiogenesis. We evaluated the microvascular density (MVD) of bone marrow biopsies (after immunostain...

journal_title：Clinical chemistry and laboratory medicine

authors： Alexandrakis MG,Passam FJ,Ganotakis E,Dafnis E,Dambaki C,Konsolas J,Kyriakou DS,Stathopoulos E

更新日期：2004-01-01 00:00:00

abstract：:Although affinity and kinetic measurements on their own provide useful information regarding the suitability of antibodies for various immunoassays, a thermodynamic analysis provices additional information that throws light on the molecular forces at work in the antigen-antibody interaction. It may then be possible to...

journal_title：Clinical chemistry and laboratory medicine

authors： Van Regenmortel MH

更新日期：1998-06-01 00:00:00

abstract：BACKGROUND:Sequencing based mutation screening assays of genes encompassing large numbers of exons could be substantially optimized by multiplex PCR, which enables simultaneous amplification of many targets in one reaction. In the present study, a multiplex PCR protocol originally developed for fragment analysis was ev...

journal_title：Clinical chemistry and laboratory medicine

authors： Horn MP,Mäder-Heinemann G,Andrey G,Largiadèr CR

更新日期：2009-01-01 00:00:00

abstract：:The effect of solvent conditions on the aggregation of IgM in serum specimens from patients with macroglobulinemia was studied by a turbidimetric procedure. Aggregation of IgM varied considerably among the samples and was affected by a number of experimental parameters. In general, IgM aggregation was more pronounced ...

journal_title：Clinical chemistry and laboratory medicine

authors： Sharma L,Baker J,Brooks AM,Sharma A

更新日期：2000-08-01 00:00:00

abstract：:Background The present study was undertaken to evaluate the clinical impact of a thyroglobulin (Tg) minirecovery test (Tg-mRec) in a large series of differentiated thyroid carcinoma (DTC) patients treated and monitored homogeneously in a tertiary referral center. Methods Included were 1120 serum samples from 798 DTC p...

journal_title：Clinical chemistry and laboratory medicine

authors： Giovanella L,Verburg FA,Trimboli P,Imperiali M,Keller F,Ceriani L

更新日期：2019-07-26 00:00:00

abstract：:Thrombophilia can be identified in about half of all patients presenting with venous thromboembolism (VTE). Thrombophilia screening for various indications has increased tremendously, but whether the results of such tests help in the clinical management of patients is uncertain. Here, current recommendations for throm...

journal_title：Clinical chemistry and laboratory medicine

authors： Stegnar M

更新日期：2010-12-01 00:00:00

abstract：:Polymorphisms of the gene encoding apolipoprotein E have been implicated in the pathogenesis of peripheral and coronary artery disease and neurodegenerative disorders such as sporadic and late-onset familial forms of Alzheimer's disease. We have developed TaqMan assay systems for the single nucleotide polymorphisms -2...

journal_title：Clinical chemistry and laboratory medicine

authors： Koch W,Ehrenhaft A,Griesser K,Pfeufer A,Müller J,Schömig A,Kastrati A

更新日期：2002-11-01 00:00:00

abstract：:Total prostate-specific antigen (PSA) and complexed PSA were determined in venous blood from 12 patients with prostate cancer before and after radical prostatectomy by using Immuno 1 PSA assays. The elimination kinetics of complexed PSA were compared with that of total PSA. Nearly constant concentrations of complexed ...

journal_title：Clinical chemistry and laboratory medicine

authors： Stephan C,Jung K,Brux B,Lein M,Sinha P,Schnorr D,Loening SA

更新日期：2000-04-01 00:00:00

abstract：BACKGROUND:The presence of preanalytical mistakes (PM) in samples from primary care centres (PCC) is a widely studied topic. Different correcting strategies have been proposed, with variable success. We planned a series of multidisciplinary sessions for clinical update, with the aim to decrease PM rates in samples from...

journal_title：Clinical chemistry and laboratory medicine

authors： Romero A,Gómez-Salgado J,Gómez-Fernández JA,Cobos A,Ruiz-Frutos C

更新日期：2017-10-26 00:00:00

abstract：:Molecular diagnostics is one of the most rapidly growing areas of laboratory medicine. This rapid growth of clinical molecular tests has outpaced the availability and development of reference methods and reference materials. Such methods and materials are important for the development, validation, and interpretation o...

journal_title：Clinical chemistry and laboratory medicine

authors： Payne DA,Mamotte CD,Gancberg D,Pazzagli M,van Schaik RH,Schimmel H,Rousseau F,IFCC Committee for Molecular Diagnostics (C-MD).

更新日期：2010-11-01 00:00:00

abstract：:In our efforts to develop diagnostic tests for complex multifactorial disorders, and to assist the research community in evaluating genetic markers for predisposition to cardiovascular disease, we have developed a prototype assay to genotype up to 35 variable sites among 15 genes. The candidate markers in this panel w...

journal_title：Clinical chemistry and laboratory medicine

authors： Cheng S,Pallaud C,Grow MA,Scharf SJ,Erlich HA,Klitz W,Pullinger CR,Malloy MJ,Kane JP,Siest G,Visvikis S

更新日期：1998-08-01 00:00:00

abstract：BACKGROUND:Insulin-like growth factors (IGFs) are believed to be important in brain development and repair following neuronal damage. It is also speculated that IGFs are involved in the association of foetal and pre-adult growth with schizophrenia (SZ). METHODS:The aim of this study was to assess levels of IGF-I, IGF-...

journal_title：Clinical chemistry and laboratory medicine

authors： Akanji AO,Ohaeri JU,Al-Shammri SA,Fatania HR

更新日期：2007-01-01 00:00:00

abstract：Background:Anti-mitochondrial autoantibodies (AMA) detected by indirect immunofluorescence (IIF) on rodent tissues are the diagnostic marker of primary biliary cholangitis (PBC). However, up to 15% of patients with PBC are AMA-negative by IIF. In the effort to close the serological gap and improve the diagnostic sensit...

journal_title：Clinical chemistry and laboratory medicine

authors： Villalta D,Seaman A,Tiongson M,Warren C,Bentow C,Bizzaro N,Alessio MG,Porcelli B,Norman GL,Mahler M

更新日期：2020-04-10 00:00:00

abstract：BACKGROUND:We report a Dutch-Belgian multicentre evaluation of the Tosoh HLC-723G8 glycohaemoglobin analyser, an ion-exchange HPLC instrument for the separation and quantification of haemoglobin A1c (HbA1c) in whole blood. METHODS:We evaluated the analytical performances of the Tosoh G8 analyser and compared the resul...

journal_title：Clinical chemistry and laboratory medicine

authors： Chapelle JP,Teixeira J,Maisin D,Assink H,Barla G,Stroobants AK,Delzenne B,van den Eshof W

更新日期：2010-03-01 00:00:00

abstract：BACKGROUND:The objective of the work was to study the manifestations of antiphospholipid syndrome (APS) in the Chinese population, and evaluate the ability of lupus anticoagulant (LAC) assays to identify APS. METHODS:Consecutive patients with APS from 2012 to 2015 in our hospital were recruited, strictly following the...

journal_title：Clinical chemistry and laboratory medicine

authors： Tang N,Sun Z,Yin S

更新日期：2016-11-01 00:00:00

abstract：Background:Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based assays are employed in more and more clinical laboratories to quantify steroids. The steroid quantification by LC-MS/MS shows great value in screening or diagnosing endocrine disorders; however, the number of functional steroids included in the ...

journal_title：Clinical chemistry and laboratory medicine

authors： Wang Z,Wang H,Peng Y,Chen F,Zhao L,Li X,Qin J,Li Q,Wang B,Pan B,Guo W

更新日期：2020-02-21 00:00:00

abstract：:The objective of this study was to establish reference intervals for thyroid stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3), total thyronine (TT4) and total triiodothyronine (TT3) on the Architect i2000 analyser (Abbott). Serum samples were obtained from apparently healthy adults (n=217, ...

journal_title：Clinical chemistry and laboratory medicine

authors： Hubl W,Schmieder J,Gladrow E,Demant T

更新日期：2002-02-01 00:00:00

abstract：:Primary biliary cholangitis (PBC), also known as primary biliary cirrhosis, is an autoimmune disease of the liver characterized by anti-mitochondrial antibodies (AMA) in 90%-95% of patients. The aim of this study was to evaluate the diagnostic value of several serum biomarkers in patients with PBC but negative for AMA...

journal_title：Clinical chemistry and laboratory medicine

authors： de Liso F,Matinato C,Ronchi M,Maiavacca R

更新日期：2017-11-27 00:00:00

abstract：:The effects of the time of the day and breakfast on the outcome of interleukin-6 and its soluble receptor in serum were studied on 22 subjectively health volunteers. We collected blood specimens at 8.00 a.m. after an overnight fast, at 9.30 a.m. after usual breakfast, and at 11.30 a.m. A second group of volunteers (n ...

journal_title：Clinical chemistry and laboratory medicine

authors： Dugué B,Leppänen E

更新日期：1998-05-01 00:00:00

abstract：:Venous blood sampling (phlebotomy) is the most common invasive procedure performed in patient care. Guidelines on the correct practice of phlebotomy are available, including the H3-A6 guideline issued by the Clinical Laboratory Standards Institute (CLSI). As the quality of practices and procedures related to venous bl...

journal_title：Clinical chemistry and laboratory medicine

authors： van Dongen-Lases EC,Cornes MP,Grankvist K,Ibarz M,Kristensen GB,Lippi G,Nybo M,Simundic AM,Working Group for Preanalytical Phase (WG-PRE), European Federation of Clinical Chemistry and Laboratory Medicine (EFLM).

更新日期：2016-07-01 00:00:00

abstract：BACKGROUND:Accurate and early diagnosis of tuberculosis (TB) is of major importance in the management and control of TB. Because the conventional bacteriological diagnosis of TB has several limitations, nucleic acid amplification (NAA) tests have emerged as promising alternatives. A potential problem with NAA tests is ...

journal_title：Clinical chemistry and laboratory medicine

authors： Qin L,Zheng R,Fan C,Cai J,Liu Z,Wang J,Lu J,Jin R,Yang H,Cui Z,Feng Y,Hu Z

更新日期：2010-10-01 00:00:00

abstract：:The recent revision of ISO15189 has further strengthened its position as the standard for accreditation for medical laboratories. Both for laboratories and their customers it is important that the scope of such accreditation is clear. Therefore the European co-operation for accreditation (EA) demands that the national...

journal_title：Clinical chemistry and laboratory medicine

authors： Thelen MH,Vanstapel FJ,Kroupis C,Vukasovic I,Boursier G,Barrett E,Bernabeu Andreu F,Brguljan PM,Brugnoni D,Lohmander M,Sprongl L,Vodnik T,Ghita I,Vaubourdolle M,Huisman W,Working Group Accreditation ISO\/CEN standards (WG-A

更新日期：2015-07-01 00:00:00