Abstract:
:In human embryonic kidney (HEK) cells stably transfected with green fluorescent protein targeted to the endoplasmic reticulum (ER), elevation of intracellular Ca2+ ([Ca2+]i) altered ER morphology, making it appear punctate. Electron microscopy revealed that these punctate structures represented circular and branched rearrangements of the endoplasmic reticulum, but did not involve obvious swelling or pathological fragmentation. Activation of protein kinase C with phorbol 12-myristate 13-acetate (PMA), prevented the effects of ionomycin on ER structure without affecting the elevation of [Ca2+]i. These results suggest that protein kinase C activation alters cytoplasmic or ER components underlying the effects of high [Ca2+]i on ER structure. Treatment of HEK cells with PMA also reduced the size of the thapsigargin-sensitive Ca2+ pool and inhibited Ca2+ entry in response to thapsigargin. Thus, protein kinase C activation has multiple actions on the calcium storage and signalling function of the endoplasmic reticulum in HEK cells: (1) reduced intracellular Ca2+ storage capacity, (2) inhibition of capacitative Ca2+ entry, and (3) protection of the endoplasmic reticulum against the effects of high [Ca2+]i.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Ribeiro CM,McKay RR,Hosoki E,Bird GS,Putney JW Jrdoi
10.1054/ceca.2000.0108keywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
175-85issue
3eissn
0143-4160issn
1532-1991pii
S0143-4160(00)90108-8journal_volume
27pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::Antisense oligodeoxynucleotides (AS-ODNs) were used in combination with transient functional expression of the cardiac Na(+)-Ca2+ exchanger (NCX1) to correlate suppression of the Na(+)-Ca2+ exchange function with down-regulation of NCX1 protein expression. In a de-novo expression system (Sf9 cells), a decrease in both...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2004.10.006
更新日期:2005-03-01 00:00:00
abstract::Cytosolic Ca(2+) signals are fundamental for the early and late steps of myoblast differentiation and are, as in many cells, generated by Ca(2+) release from internal stores as well as by plasma membrane Ca(2+) entry. Our recent studies identified the store-operated Ca(2+) channels, Orai1 and TRPC1&C4, as crucial for ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.10.014
更新日期:2014-12-01 00:00:00
abstract::Numerous lines of evidence indicate that nuclear calcium concentration ([Ca2+]n) may be controlled independently from cytosolic events by a local machinery. In particular, the perinuclear space between the inner nuclear membrane (INM) and the outer nuclear membrane (ONM) of the nuclear envelope (NE) likely serves as a...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102143
更新日期:2020-03-01 00:00:00
abstract::Amyloid beta (Aβ) oligomers accumulate in the brain tissue of Alzheimer disease patients and are related to disease pathogenesis. The precise mechanisms by which Aβ oligomers cause neurotoxicity remain unknown. We recently reported that Aβ oligomers cause intracellular Ca(2+) overload and neuronal death that can be pr...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2011.02.001
更新日期:2011-03-01 00:00:00
abstract::The sphingosine derivatives sphingosylphosphorylcholine (SPC) and sphingosine-1-phosphate (S1P) caused a similar elevation of the intracellular Ca2+ concentration ([Ca2+]i) in an immortalized airway epithelial cell line (CFNP9o-) incubated in Ca(2+)-free medium. The maximal effect was obtained with 2 microM SPC and 0....
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90095-1
更新日期:1998-06-01 00:00:00
abstract::A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2005.06.012
更新日期:2005-09-01 00:00:00
abstract::Acidic organelles such as lysosomes serve as non-canonical Ca(2+) stores. The Ca(2+) mobilising messenger NAADP is thought to trigger local Ca(2+) release from such stores. These events are then amplified by Ca(2+) channels on canonical ER Ca(2+) stores to generate physiologically relevant global Ca(2+) signals. Coupl...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2015.03.006
更新日期:2015-10-01 00:00:00
abstract::The correlation between the intracellular Ca2+ concentration ([Ca2+]i) and membrane potential in monolayers of density-arrested normal rat kidney (NRK) fibroblasts was investigated. Using the fluorescent probe Fura-2, spontaneous repetitive spike-like increases in [Ca2+]i (Ca2+ spikes) were observed that were synchron...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90013-0
更新日期:1997-09-01 00:00:00
abstract::A potential role of arachidonic acid in the modulation of insulin secretion was investigated by measuring its effects on calmodulin-dependent protein kinase and protein kinase C in islet subcellular fractions. The results were interpreted in the light of arachidonic acid effects on insulin secretion from intact islets...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90044-s
更新日期:1992-03-01 00:00:00
abstract::Apparent calcium stability constants and the purity of Ca-chelating agents were evaluated using Ca(2+)-selective electrodes by the double-log optimization method [Oiki S. Yamamoto T. Okada Y. (1994) Cell Calcium, 15, 199-208]. The method was amended to allow evaluation of the free Ca2+ concentrations contaminating ele...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90060-4
更新日期:1994-03-01 00:00:00
abstract::An inward current responsible for hormone regulated Ca2+ entry has been identified in cultured rat hepatocytes using whole cell patch clamp. Addition of 20 nM vasopressin or of 100 microM ATP induced the inward current, which could be observed more clearly after blocking an outward K+ current. This large outward K+ cu...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90042-x
更新日期:1995-07-01 00:00:00
abstract::The endoplasmic reticulum (ER) acts as a dynamic calcium store and is involved in the generation of specific patterns of calcium signals in neurons. Calcium is mobilized from the ER store by multiple signaling cascades, and neuronal activity is known to regulate ER calcium levels. We asked how neurons regulate ER calc...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.04.002
更新日期:2015-08-01 00:00:00
abstract::Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90113-x
更新日期:1996-05-01 00:00:00
abstract::The annexins are a family of calcium-binding proteins. Data from protein and cDNA sequencing have shown that at least five distinct but closely related mammalian annexins exist each of which possesses four or eight homologous internal repeats which may be calcium-and phospholipid-binding domains. The proteins are pres...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(89)90038-9
更新日期:1989-01-01 00:00:00
abstract::NAADP has been shown to act as a second messenger in a wide range of systems from plants to mammalian cells. Although it had always been considered as a canonical second messenger, recent work has shown that it is also active when applied extracellularly. It has also been suggested that NAADP might have a direct actio...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2006.10.002
更新日期:2007-06-01 00:00:00
abstract::Odontoblasts are involved in the transduction of stimuli applied to exposed dentin. Although expression of thermo/mechano/osmo-sensitive transient receptor potential (TRP) channels has been demonstrated, the properties of TRP vanilloid 1 (TRPV1)-mediated signaling remain to be clarified. We investigated physiological ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2012.05.002
更新日期:2012-08-01 00:00:00
abstract::Transient spontaneous increases in the intracellular Ca2+ concentration have been frequently observed in astrocytes in cell culture and in acutely isolated slices from several brain regions. Recent in vivo experiments, however, reported only a low frequency of spontaneous Ca2+ events in astrocytes. Since the ex vivo e...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.06.002
更新日期:2008-03-01 00:00:00
abstract::R24571 a derivative of the antimycotic miconazole, appears to be 5 to 8 times more potent than trifluoperazine in its ability to inhibit the calmodulin-dependent phosphorylation of cardiac sarcoplasmic reticulum vesicles. The cAMP-dependent protein kinase mediated phosphorylation of cardiac sarcoplasmic reticulum was ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(83)90039-8
更新日期:1983-04-01 00:00:00
abstract::Pancreatic islets produce pulses of insulin and other hormones that maintain normal glucose homeostasis. These micro-organs possess exquisite glucose-sensing capabilities, allowing for precise changes in pulsatile insulin secretion in response to small changes in glucose. When communication among these cells is disrup...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102081
更新日期:2019-11-01 00:00:00
abstract::Polycystin-2, a member of the TRP family of calcium channels, is encoded by the human PKD2 gene. Mutations in that gene can lead to swelling of nephrons into the fluid-filled cysts of polycystic kidney disease. In addition to expression in tubular epithelial cells, human polycystin-2 is found in muscle and neuronal ce...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2005.03.003
更新日期:2005-06-01 00:00:00
abstract::Antigen-mediated mast cell (MC) degranulation is the critical early event in the induction of allergic reactions. Transient receptor potential channels (TRPC), particularly TRPC1, are thought to contribute to such MC activation. To explore the contribution of TRPC1 in MC-driven allergic reactions, we examined antigen-...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2013.02.001
更新日期:2013-05-01 00:00:00
abstract::The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examine...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.2001.0233
更新日期:2001-10-01 00:00:00
abstract::Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.11.001
更新日期:2012-03-01 00:00:00
abstract::Pituitary neuropeptide oxytocin is increasingly recognised as a cardiovascular hormone, in addition to its many regulatory roles in other organ systems. Studies in atrial and ventricular myocytes from the neonatal and adult rats have identified synthesis of oxytocin and the expression of oxytocin receptors in these ce...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102099
更新日期:2019-12-01 00:00:00
abstract::N,N'-dicyclohexylcarbodiimide (DCCD) and 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide (CMCD) inhibited calmodulin-dependent Ca2(+)+Mg2(+)-ATPase activity in erythrocyte ghost membranes. The extent of the inhibition caused by carbodiimides strongly depended on their hydrophobicity. Hydrophobic DCCD was a more potent...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90004-e
更新日期:1990-04-01 00:00:00
abstract::Rat liver was homogenized and subjected to differential centrifugation. When the low speed nuclear pellet was processed on a Percoll gradient, plasma membrane markers and Ins(1,4,5)P3 binding activity purified together. The high speed (microsomal) fraction was subfractionated by sucrose density gradient centrifugation...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90007-s
更新日期:1993-06-01 00:00:00
abstract::Cultured human skeletal muscle cells are frequently used as a model to study muscle pathology, in which Ca2+ homeostasis might be affected. However, their excitation-contraction (E-C) coupling has been poorly investigated. In order to elucidate E-C coupling of cultured muscle cells, we activated the acetylcholine rece...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90099-3
更新日期:1997-01-01 00:00:00
abstract::Human neutrophils, preloaded with the fluorescent probe, Fura-2, were exposed to Ca2+-releasing agents. The monitored traces of fluorescence were transformed by computer to cytosolic Ca2+ concentration ([ Ca2+]i). Due to quenching of Fura-2, the addition of Mn2+ enabled us to compute the cytosolic concentration of tot...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(89)90025-0
更新日期:1989-10-01 00:00:00
abstract::The fluo family of indicators is frequently used in studying Ca(2+) physiology; however, choosing which fluo indicator to use is not obvious. Indicator properties are typically determined in well-defined aqueous solutions. Inside cells, however, the properties can change markedly. We have characterized each of three f...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2012.05.010
更新日期:2012-08-01 00:00:00
abstract::The control of calcium homeostasis is essential for cell survival and is of crucial importance for several physiological functions. The discovery of the epithelial calcium channel Transient Receptor Potential Vaniloid (TRPV6) in intestine has uncovered important Ca(2+) absorptive pathways involved in the regulation of...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.02.007
更新日期:2008-11-01 00:00:00