Genes upregulated during castration-induced rat prostatic apoptosis: cloning and characterization of new cDNAs.

Abstract:

OBJECTIVE:To isolate new cDNAs corresponding to genes whose expression is increased during castration-induced rat prostate apoptosis. MATERIALS AND METHODS:Differential display of mRNAs from 3-day castrated and normal rat ventral prostates was used to identify differentially expressed clones. Northern blots were hybridized to confirm the positive regulation of the candidates and to follow the change in their expression in the involuting rat prostate, and in thymocytes of dexamethazone-treated rats. RESULTS:Five cDNAs were cloned: one encoding ribosomal protein L7, one coding for the insulin-like growth factor binding protein-3 (IGFBP-3), and three whose products are unknown. After castration, all five genes had expression kinetics that closely paralleled the proportion of prostatic epithelial cells undergoing apoptosis. The gene encoding L7 and two of the unknown genes were also upregulated in glucocorticoid-induced programmed death in thymocytes. In addition to the IGFBP-3 gene, those coding for proteins IGFBP-4, -5 and -6 were also overexpressed in the involuting prostate of androgen-deprived rats. CONCLUSION:Five new genes were identified that are up-regulated during castration-induced rat prostate apoptosis, three of which are potentially involved in the common intracellular pathway leading to programmed cell death.

journal_name

BJU Int

journal_title

BJU international

authors

Bruyninx M,Ammar H,Reiter E,Cornet A,Closset J

doi

10.1046/j.1464-410x.2000.00654.x

keywords:

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

1134-42

issue

9

eissn

1464-4096

issn

1464-410X

pii

bju654

journal_volume

85

pub_type

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