Abstract:
:Human granulocytic ehrlichiosis (HGE) is an emerging febrile systemic disease caused by the HGE agent, an obligatory intracellular bacterium of granulocytes. The pathogenicity- and immunity-related mechanisms of HGE are unknown. In this study, several cytokines generated in human peripheral blood leukocytes (PBLs) incubated with the HGE agent or a recombinant 44-kDa major surface protein (rP44) of the HGE agent were examined by reverse transcription-PCR and a capture enzyme-linked immunosorbent assay. The HGE agent induced expression of interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha), and IL-6 mRNAs and proteins in PBLs in a dose-dependent manner to levels as high as those resulting from Escherichia coli lipopolysaccharide stimulation. The kinetics of induction of these three cytokines in PBLs by rP44 and by the HGE agent were similar. Proteinase K treatment of the HGE agent or rP44 eliminated the ability to induce these three cytokines. Induction of these cytokine mRNAs was not dependent on superoxide generation. These results suggest that P44 proteins have a major role in inducing the production of proinflammatory cytokines by PBLs. Expression of IL-8, IL-10, gamma interferon, transforming growth factor beta, and IL-2 mRNAs in response to the HGE agent was not remarkable. Among PBLs, neutrophils and lymphocytes expressed IL-1beta mRNA but not TNF-alpha or IL-6 mRNA in response to the HGE agent, whereas monocytes expressed all three of these cytokine mRNAs. These observations suggest that induction of proinflammatory-cytokine gene expression by the major outer membrane protein of the HGE agent in monocytes, which are not the primary host cells of the HGE agent, contributes to HGE pathogenesis and immunomodulation.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Kim HY,Rikihisa Ydoi
10.1128/iai.68.6.3394-3402.2000keywords:
subject
Has Abstractpub_date
2000-06-01 00:00:00pages
3394-402issue
6eissn
0019-9567issn
1098-5522journal_volume
68pub_type
杂志文章abstract::We have constructed an improved recombination-based in vivo expression technology (RIVET) and used it as a screening method to identify Vibrio cholerae genes that are transcriptionally induced during infection of infant mice. The improvements include the introduction of modified substrate cassettes for resolvase that ...
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1663-1667.1989
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journal_title:Infection and immunity
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doi:10.1128/IAI.64.4.1272-1283.1996
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.90-98.1979
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journal_title:Infection and immunity
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doi:10.1128/IAI.3.6.756-761.1971
更新日期:1971-06-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.40.3.1218-1222.1983
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journal_title:Infection and immunity
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doi:10.1128/IAI.13.2.438-447.1976
更新日期:1976-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.609-621.1995
更新日期:1995-02-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.59.1.398-406.1991
更新日期:1991-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.516-522.1978
更新日期:1978-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01690-06
更新日期:2007-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.9.2450-2457.1988
更新日期:1988-09-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.4.516-518.1970
更新日期:1970-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6891-6895.2000
更新日期:2000-12-01 00:00:00
abstract::Borrelia burgdorferi, the agent of Lyme disease, is transmitted by ticks. During transmission from the tick to the host, spirochetes are delivered with tick saliva, which contains the salivary protein Salp15. Salp15 has been shown to protect spirochetes against B. burgdorferi-specific antibodies. We now show that Salp...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00