Abstract:
:Interleukin-16 (IL-16) acts as a chemoattractant for CD4+ cells, as a modulator of T-cell activation, and plays a key role in asthma. This report describes the cytokine-inducing effects of IL-16 on total peripheral blood mononuclear cells (PBMC) and PBMC subpopulations. While CD4+ T lymphocytes did not secrete cytokines in response to rhIL-16, CD14+ CD4+ monocytes and maturing macrophages secrete IL-1beta, IL-6, IL-15 and tumour necrosis factor-alpha (TNF-alpha) upon rhIL-16 stimulation. The mRNA species for these four cytokines were detected as early as 4 hr post-stimulation, with protein being secreted by 24 hr. Secretion of IL-1beta and IL-6 by total PBMC was dose dependent, with maximal secretion being observed using 50 ng/ml rhIL-16. However, for IL-15 or TNF-alpha maximal secretion by total PBMC occurred with all concentrations between 5 ng/ml to 500 ng/ml rhIL-16. Purified monocytes/macrophages secreted maximal concentrations of all four cytokines in the presence of 500 ng/ml rhIL-16, except for monocytes where maximal secretion of IL-15 was, interestingly, observed with only 50 ng/ml rhIL-16. The use of higher concentrations of rhIL-16 (1000 ng/ml) inhibited secretion of all four cytokines. While these IL-16-induced cytokines are likely to be involved in the immune system's response to antigen, the data suggest that IL-16 may play a key role in initiating and/or sustaining an inflammatory response.
journal_name
Immunologyjournal_title
Immunologyauthors
Mathy NL,Scheuer W,Lanzendörfer M,Honold K,Ambrosius D,Norley S,Kurth Rdoi
10.1046/j.1365-2567.2000.00997.xkeywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
63-9issue
1eissn
0019-2805issn
1365-2567pii
imm997journal_volume
100pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::The recovery of the B-cell population in adult thymectomized, irradiated and bone marrow-reconstituted mice (T X BM mice was estimated at various times after bone marrow transplantation. The spleen cells to be tested were mixed with dexamethasone-resistant thymocytes (DRT) and sheep red blood cells (SRBC) and transfer...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::Large numbers of cells can be recovered from pig intestine with phenotypes suggesting lamina propria rather than intraepithelial origin. Following activation with concanavalin A these cells produced a T-cell growth factor (TCGF) activity which was not inhibited in the presence of a monoclonal antibody recognizing pig ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::Antibodies to neuronal antigens are associated with many neurological diseases including paraneoplastic neurological disorders, epilepsy, amyotrophic lateral sclerosis and multiple sclerosis. Immunization with neuronal antigens such as neurofilament light (NF-L), a neuronal intermediate filament in axons, has been sho...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12797
更新日期:2017-12-01 00:00:00
abstract::The decay-accelerating factor (DAF) contains four complement control protein repeats (CCPs) with a single N-linked glycan positioned between CCPs 1 and 2. In previous studies we found that the classical pathway regulatory activity of DAF resides in CCPs 2 and 3 while its alternative pathway regulatory activity resides...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00086.x
更新日期:2000-09-01 00:00:00
abstract::Forssman antiserum produced in rabbits immunized with sheep erythrocyte stromata was found to contain an IgG antibody which inhibited both passive anaphylactic sensitization of guinea-pig lung and also histamine-releasing activity of soluble immune complexes. This Forssman antibody did not itself cause histamine rele...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::T-cell mediated immunity (CMI) is crucial for protection against genital chlamydial infection in mice. To define the underlying molecular mechanism for this protection, several T-cell clones generated against the Chlamydia trachomatis agent of mouse pneumonitis (MoPn) were analysed in an in vitro model of the mucosal ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Corneal allografts contain few or no Ia+ Langerhans' cells and consistently fail to elicit allospecific delayed-type hypersensitivity (DTH). The present study examined the hypothesis that the inability of corneal allografts to induce allospecific DTH was the result of active suppression. However, the results indicated...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::This study describes a subtractive hybridization analysis to identify differences in gene expression between sibling Th memory clones, elicited by virus infection and expressing identical T-cell receptor (TCR)-alphabeta rearrangements but distinct lymphokine phenotype: clone Bpp9 secretes interleukin (IL)-4, IL-5 and ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01953.x
更新日期:2004-10-01 00:00:00
abstract::Therapeutic vaccination using T-cell receptor (TCR) peptides from V genes commonly expressed by potentially pathogenic T cells remains an approach of interest for treatment of multiple sclerosis (MS) and other autoimmune diseases. We developed a trivalent TCR vaccine containing complementarity determining region (CDR)...
journal_title:Immunology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1365-2567.2007.02703.x
更新日期:2008-01-01 00:00:00
abstract::This study reports the expression and functional characterization of the rat analogue of the human complement regulatory molecule CD59. We here describe the expression in chinese hamster ovary (CHO) cells of rat CD59 and a modified rat CD59 in which an N-glycosylation site at Asn-16 has been deleted by point mutation....
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00200.x
更新日期:1997-04-01 00:00:00
abstract::Much of the pathology of malaria may be due to the interactions of cytokines, especially tumour necrosis factor (TNF), with various cell types, including endothelial cells, with consequent widespread systemic effects. It has been shown previously that heat-stable exoantigens in the supernatants of blood-stage parasite...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::Innate immune responses vary in a circadian manner, and more recent investigations aim to understand the underlying molecular mechanisms. Cytokine production varies significantly over the course of a day depending on the time of stimulation by pathogens or Toll-like receptor ligands, and multiple signaling pathways li...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13053
更新日期:2019-06-01 00:00:00
abstract::Lipoprotein lipase (LPL) is the key enzyme in the metabolism of triglyceride-rich lipoproteins. The patterns of LPL mRNA expression and secretion of the enzyme have not yet been established in natural killer (NK) cells. We show in the present communication that CD3- CD16+ cells (NK cells) transcribe LPL mRNA, express ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Acute and chronic infusions of a lipophilic analogue of cyclic AP (dibutyryl cyclic AMP) into cannulated afferent lymphatics of popliteal lymph nodes of sheep have reduced the output of both small and blast lymphocytes into efferent lymph and have tended to reduce the volume of efferent lymph flow (collected by chroni...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::A technique is reported here for the quantitative extraction of live cells from the lung interstitium; it involves the incubation of slices of perfused lung in a mixture containing optimal concentrations of collagenase, DNAse, and fetal calf serum, followed by the simultaneous recovery and fractionation of cells relea...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::The capacity of the immune system to respond efficiently to new antigens depends upon a continuous source of naive CD4+ T cells. Such cells exit from the thymus and join the recirculated T-cell pool. Factors present at the sites of naive CD4+ T-cell circulation must be responsible for their survival, since upon remova...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00906.x
更新日期:1999-11-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) may play an important role in the control of human papillomavirus (HPV)-induced anogenital neoplasias, but have been difficult to study owing to the difficulty in obtaining sufficient quantities of infectious virus. To address this we have stimulated human HPV-specific CTL in vitro using ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::Bovine peripheral blood monocytes were isolated from buffy coats of ACD-anticoagulated whole blood. Cells cultivated on plastic surfaces for 20 h were judged to be 95% monocytes based on nonspecific esterase-1 staining, uptake of latex particles and surface receptor characteristics. Bovine monocytes were maintained up...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::The endogenous cannabinoid system plays an important role in regulating the immune system. Modulation of endogenous cannabinoids represents an attractive alternative for the treatment of inflammatory disorders. This study investigated the effects of URB597, a selective inhibitor of fatty acid amide hydrolase (FAAH), t...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02838.x
更新日期:2008-10-01 00:00:00
abstract::The influence of intact influenza virus and purified detergent solubilized haemagglutinin (HA) subunits from these viruses on human natural killer (NK) cell activity was examined. Effector cells incubated with whole influenza virus for 18 hr initiated the production of alpha interferon which was associated with the en...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::To date, the most potent mucosal vaccine adjuvants to be identified have been bacterial toxins. The present data demonstrate that the type 2 ribosome-inactivating protein (type 2 RIP), mistletoe lectin I (ML-I) is a strong mucosal adjuvant of plant origin. A number of plant lectins were investigated as intranasal (i.n...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01157.x
更新日期:2001-01-01 00:00:00
abstract::Eosinophil recruitment to the airways, including involvement of haemopoietic eosinophil-basophil progenitors (Eo/B-CFU), is primarily regulated by interleukin-5 (IL-5) and eotaxin. In this study, we investigated the haemopoietic mechanisms in upper and lower airway eosinophilic inflammation. Ovalbumin (OVA) sensitized...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02109.x
更新日期:2005-03-01 00:00:00
abstract::Chemically cross-linked C-reactive protein (CRP) oligomers were prepared and characterized, and C1q binding and C activation were investigated. Purified human CRP was polymerized in the presence of both non-cleavable and cleavable cross-linking agents and further separated by Superose 12 analytical FPLC column chromat...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Bone-marrow-derived macrophages are divided into two phenotypically and functionally distinct subsets, M1 and M2 macrophages. Recently, it was shown that adoptive transfer of M2-polarized peritoneal macrophages reduced the severity of experimental colitis in mice. However, it is still unclear whether peritoneal macrop...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12647
更新日期:2016-11-01 00:00:00
abstract::There is increasing evidence that primitive progenitors migrate from the bone marrow (BM) via the peripheral circulation to tissue sites where they undergo in situ differentiation to provide a continued source of effector cells, such as eosinophils, during an allergic inflammatory response. To study mechanisms of prog...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01686.x
更新日期:2003-08-01 00:00:00
abstract::Group 2 innate lymphoid cells (ILC2s) produce a significant amount of interleukin-5 (IL-5), which supports eosinophil responses in various tissues; they also produce IL-13, which induces mucus production and contributes to tissue repair or fibrosis. The ILC2s are activated by alarmins, such as IL-33 released from epit...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12537
更新日期:2016-01-01 00:00:00
abstract::The ability to expand tumour-infiltrating lymphocytes in vitro has been greatly enhanced by the use of antigen-independent mechanisms of immune cell costimulation. We have produced human, using the K562 cell line, and murine, using YAC-1 cells, artificial antigen presenting cells (aAPC) and demonstrate that these cell...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01853.x
更新日期:2004-05-01 00:00:00
abstract::Mitogen-activated protein/ERK kinase kinase 3 (MEKK3) is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP3K family. Recently, we found that MEKK3 plays a critical role in interleukin-1 (IL-1) receptor and Toll-like receptor 4 signalling using established primary mouse embryonic fibroblast (MEF)...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02495.x
更新日期:2007-02-01 00:00:00
abstract::Apolipoprotein-E (apoE) is expressed at high levels by macrophages. In addition to its role in lipid transport, macrophage-derived apoE plays an important role in immunoregulation. Previous studies have identified macrophage subpopulations that differ substantially in their ability to synthesize specific cytokines and...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01972.x
更新日期:2004-11-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is a global health problem characterized by a high rate of chronic infection, which may in part be due to a defect in myeloid dendritic cells (mDCs). This defect appears to be remedied by treatment with interferon-α (IFN-α) -based antiviral therapies; however, the molecular mechanisms...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12350
更新日期:2014-12-01 00:00:00