Abstract：
:We previously proposed that the first enzyme for histidine biosynthesis in Salmonellatyphimurium plays a role in regulating expression of the histidine operon and that in order to play this role the enzyme must form a complex with histidyl-tRNA. Among the many observations that led to these conclusions were 1) that regulation of the histidine operon is defective in strains carrying a mutation in the gene for the first enzyme that renders the enzyme resistant to inhibition by histidine; and 2) that the enzyme purified from the wild type strain interacts specifically, and with high affinity, with histidyl-tRNA. The present study was carried out to test the prediction that the enzyme purified from the mutant strain described above would display a defect in its interaction with histidyl-tRNA. This prediction was fulfilled by the finding that purified histidine-insensitive enzyme does not bind histidyl-tRNA. Our results therefore suggest that the capacity of the enzyme to bind histidyl-tRNA invitro is a reflection of its regulatory function invivo.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Smith O,Meyers MM,Vogel T,Deeley RD,Goldberger Rdoi
10.1093/nar/1.7.881keywords:
subject
Has Abstractpub_date
1974-07-01 00:00:00pages
881-8issue
7eissn
0305-1048issn
1362-4962journal_volume
1pub_type
杂志文章abstract：:Biological evolution resulted in a homochiral world in which nucleic acids consist exclusively of d-nucleotides and proteins made by ribosomal translation of l-amino acids. From the perspective of synthetic biology, however, particularly anabolic enzymes that could build the mirror-image counterparts of biological mac...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx079
更新日期：2017-04-20 00:00:00
abstract：:Oligodeoxyribonucleotides incorporating non-hydrolyzable dialkyl sulfide linked thymidine dimers (TsT) were synthesized chemically by the solid-phase approach. The sulfide dimer TsT was stable to degradation by snake-venom phosphodiesterase, calf spleen phosphodiesterase, Nuclease P1 and Nuclease S1. Thermal denaturat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.6.1473
更新日期：1993-03-25 00:00:00
abstract：:Multiple regulatory modules contribute to the complex expression programs realized by many loci. Although long thought of as isolated components, recent studies demonstrate that such regulatory sequences can physically associate with promoters and with each other and may localize to specific sub-nuclear transcription ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1160
更新日期：2011-04-01 00:00:00
abstract：:Fifteen years elapsed between the discovery of the double helix (1953) and the first DNA sequencing (1968). Modern DNA sequencing began in 1977, with development of the chemical method of Maxam and Gilbert and the dideoxy method of Sanger, Nicklen and Coulson, and with the first complete DNA sequence (phage X174), whi...
journal_title：Nucleic acids research
pub_type： 历史文章,杂志文章
doi：10.1093/nar/gkm688
更新日期：2007-01-01 00:00:00
abstract：:Functionality of the non-coding transcripts encoded by the human genome is the coveted goal of the modern genomics research. While commonly relied on the classical methods of forward genetics, integration of different genomics datasets in a global Systems Biology fashion presents a more productive avenue of achieving ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw162
更新日期：2016-04-20 00:00:00
abstract：:Chromatin modulation at various cis-acting elements is critical for V(D)J recombination during T and B cell development. MARbeta, a matrix-associated region (MAR) located upstream of the T cell receptor beta (TCRbeta) enhancer (Ebeta), serves a crucial role in silencing Ebeta-mediated TCR activation. By DNaseI hyperse...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh807
更新日期：2004-09-15 00:00:00
abstract：:A new E. coli strain has been constructed that contains the dinD1::LacZ+ fusion and is deficient in methylation-dependent restriction systems (McrA-, McrBC-, Mrr-). This strain has been used to clone restriction endonuclease genes directly into E. coli. When E. coli cells are not fully protected by the cognate methyla...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.12.2399
更新日期：1994-06-25 00:00:00
abstract：:MiRNAs post-transcriptionally regulate gene expression by recruiting the miRNA-induced silencing complex (miRISC) to target mRNAs. However, the mechanisms by which miRISC components are maintained at appropriate levels for proper function are largely unknown. Here, we demonstrate that Caenorhabditis elegans TEG-1 regu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw836
更新日期：2017-02-17 00:00:00
abstract：:Long terminal repeat (LTR)-retrotransposons comprise a significant portion of the rice genome. Their complete characterization is thus necessary if the sequenced genome is to be annotated correctly. In addition, because LTR-retrotransposons can influence the expression of neighboring genes, the complete identification...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl780
更新日期：2007-01-01 00:00:00
abstract：:The SIGnaling Network Open Resource 2.0 (SIGNOR 2.0) is a public repository that stores signaling information as binary causal relationships between biological entities. The captured information is represented graphically as a signed directed graph. Each signaling relationship is associated to an effect (up/down-regul...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz949
更新日期：2020-01-08 00:00:00
abstract：:Here we describe the application of a novel combinatorial method, restriction endonuclease protection selection and amplification (REPSA), to identification of a consensus DNA binding site for the TATA binding subunit (hTBP) of the human general transcription factor TFIID. Unlike most combinatorial methods, REPSA is b...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.16.3339
更新日期：1997-08-15 00:00:00
abstract：:Orthology is a widely used concept in comparative and evolutionary genomics. In addition to prokaryotic orthology, delineating eukaryotic orthology has provided insight into the evolution of higher organisms. Indeed, many eukaryotic ortholog databases have been established for this purpose. However, unlike prokaryotes...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn227
更新日期：2008-06-01 00:00:00
abstract：:With recent advancements in techniques for cellular data acquisition, information on cellular processes has been increasing at a dramatic rate. Visualization is critical to analyzing and interpreting complex information; representing cellular processes or pathways is no exception. VISIBIOweb is a free, open-source, we...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq352
更新日期：2010-07-01 00:00:00
abstract：:The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.15.3031
更新日期：1996-08-01 00:00:00
abstract：:Budding yeast telomerase is mainly activated by Tel1/Mec1 (yeast ATM/ATR) on Cdc13 from late S to G2 phase of the cell cycle. Here, we demonstrated that the telomerase-recruitment domain of Cdc13 is also phosphorylated by Cdk1 at the same cell cycle stage as the Tel1/Mec1-dependent regulation. Phosphor-specific gel an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp235
更新日期：2009-06-01 00:00:00
abstract：:Here we examined the role of cellular vitamin C in genotoxicity of carcinogenic chromium(VI) that requires reduction to induce DNA damage. In the presence of ascorbate (Asc), low 0.2-2 microM doses of Cr(VI) caused 10-15 times more chromosomal breakage in primary human bronchial epithelial cells or lung fibroblasts. D...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl1069
更新日期：2007-01-01 00:00:00
abstract：:This work describes the organization, at the nucleotide sequence level, of genes flanking the junctions of the large single copy regions and the inverted repeats of Spinacia oleracea (spinach) and Nicotiana debneyi chloroplast DNAs. In both genomes, trnH1, the gene for tRNA-His(GUG) is located at the extremity of the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.16.6547
更新日期：1984-08-24 00:00:00
abstract：:The effect of amino groups attached at positions 2 and 8 of the hypoxanthine moiety in the structure, reactivity and stability of DNA duplexes and triplexes is studied by means of quantum mechanical calculations, as well as extended molecular dynamics (MD) and thermodynamic integration (MD/TI) simulations. Theoretical...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.12.2522
更新日期：2001-06-15 00:00:00
abstract：:Studies involving ribozyme-directed inactivation of targeted RNA molecules have met with mixed success, making clear the importance of methods to measure and optimize ribozyme activity within cells. The interpretation of biochemical assays for determining ribozyme activity in the cellular environment have been complic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.15.3494
更新日期：1998-08-01 00:00:00
abstract：:Pluripotency, the ability of embryonic stem cells to differentiate into specialized cell types, is determined by ESC-specific gene regulators such as transcription factors and chromatin modification factors. It is not well understood how ESCs are poised for differentiation, however, and methods are needed for prognosi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx752
更新日期：2017-10-13 00:00:00
abstract：:Chromatin boundaries regulate gene expression by modulating enhancer-promoter interactions and insulating transcriptional influences from organized chromatin. However, mechanistic distinctions between these two aspects of boundary function are not well understood. Here we show that SF1, a chromatin boundary located in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp362
更新日期：2009-07-01 00:00:00
abstract：:The catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs) is a member of a sub-family of phosphatidylinositol (PI) 3-kinases termed PIK-related kinases. A distinguishing feature of this sub-family is the presence of a conserved C-terminal region downstream of a PI 3-kinase domain. Mutants defective in DNA-P...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.8.1965
更新日期：1998-04-15 00:00:00
abstract：:Current approaches to design efficient antisense RNAs (asRNAs) rely primarily on a thermodynamic understanding of RNA-RNA interactions. However, these approaches depend on structure predictions and have limited accuracy, arguably due to overlooking important cellular environment factors. In this work, we develop a bio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx115
更新日期：2017-05-19 00:00:00
abstract：:We have formed complexes of relaxed closed circular Col E1 DNA with various combinations of histones, and examined the effects of treating the complexes with nicking-closing enzyme. Germond et al (1) have shown that when a mixture of the four core histones of the nucleosome (HIA, H2B, H3 and H4) is used in such an exp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.5.1159-a
更新日期：1977-01-01 00:00:00
abstract：:During S phase, the entire genome must be precisely duplicated, with no sections of DNA left unreplicated. Here, we develop a simple mathematical model to describe the probability of replication failing due to the irreversible stalling of replication forks. We show that the probability of complete genome replication i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt728
更新日期：2013-11-01 00:00:00
abstract：:cDNA expression libraries displayed on lambda phage have been successfully employed to identify partners involved in antibody-antigen, protein- protein and DNA-protein interactions and represent a novel approach to functional genomics. However, as in all other cDNA expression libraries based on fusion to a carrier pol...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnf077
更新日期：2002-08-01 00:00:00
abstract：:TP63, a member of the p53 gene family gene, encodes the ΔNp63 protein and is one of the most frequently amplified genes in squamous cell carcinomas (SCC) of the head and neck (HNSCC) and lungs (LUSC). Using an epiallelic series of siRNAs with intrinsically different knockdown abilities, we show that the complete loss ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw036
更新日期：2016-04-20 00:00:00
abstract：:Several proteins that have substantially diverged during evolution retain similar three-dimensional structures and biological function inspite of poor sequence identity. The database on Genomic Distribution of protein structural domain Superfamilies (GenDiS) provides record for the distribution of 4001 protein domains...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki087
更新日期：2005-01-01 00:00:00
abstract：:The functionalisation of oligodeoxynucleotides and oligoribonucleotides by incorporation of 2'-amido-2'-deoxyribonucleosides, possibly containing a reporter group via the 2'-amido bond, was examined. Therefore 2'-acetamido-ribonucleosides containing a small methyl group at the 2'-amido bond were synthesized as model c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.1.51
更新日期：1995-01-11 00:00:00
abstract：:Linked-atom molecular modelling was employed to determine the steric and torsional requirements for intercalation of proflavine into a double-stranded region of DNA compatible with adjacent regions of cohelical A-DNA. The optimum intercalation conformation is characterized by the dihedral angles xi and psi becoming tr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.11.3855
更新日期：1977-11-01 00:00:00
