Stereochemical model for proflavin intercalation in A-DNA.

abstract：:Signal transduction ATPases with numerous domains (STAND) get activated through inducer-dependent assembly into multimeric platforms. This switch relies on the conversion of their nucleotide-binding oligomerization domain (NOD) from a closed, ADP-bound form to an open, ATP-bound form. The NOD closed form is stabilized...

journal_title：Nucleic acids research

authors： Danot O

更新日期：2015-03-31 00:00:00

abstract：:Existence of some extra-genetic (epigenetic) codes has been postulated since the discovery of the primary genetic code. Evident effects of histone post-translational modifications or DNA methylation over the efficiency and the regulation of DNA processes are supporting this postulation. EMdeCODE is an original algorit...

journal_title：Nucleic acids research

authors： Santoni FA

更新日期：2013-02-01 00:00:00

abstract：:A synthetic gene for somatomedin C, a human growth factor, has been assembled by a single ligation of 23 oligodeoxyribonucleotides, which were chemically synthesized by an improved solid phase phosphotriester method. ...

journal_title：Nucleic acids research

authors： Sproat BS,Gait MJ

更新日期：1985-04-25 00:00:00

abstract：:The rabbit homolog to the locus control region (LCR) of the human beta-like globin gene cluster was isolated, and long segments containing the DNase I hypersensitive sites (HS) were sequenced. The order and spacing of HS4, HS3, HS2 and HS1 are conserved between rabbit and human. Alignment of these sequences with their...

journal_title：Nucleic acids research

authors： Hardison R,Xu J,Jackson J,Mansberger J,Selifonova O,Grotch B,Biesecker J,Petrykowska H,Miller W

更新日期：1993-03-11 00:00:00

abstract：:The MspI restriction-modification system, which recognizes the sequence 5'-CCGG-3', has been previously cloned and sequenced (1). We subcloned the methyltransferase gene (M.MspI) downstream of the ptac promoter in the multicopy vector pUC119 and overexpressed it in E. coli. Upon induction with IPTG, M.MspI constitutes...

journal_title：Nucleic acids research

authors： Dubey AK,Mollet B,Roberts RJ

更新日期：1992-04-11 00:00:00

abstract：:Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguan...

journal_title：Nucleic acids research

authors： Agris PF,Schmidt PG

更新日期：1980-05-10 00:00:00

abstract：:A peptide initiation factor purified from rat liver and promoting the binding of initiator tRNA and model initiators to 40S and 80S ribosome at an acid pH liberates methionine and N-acetylmethionine from Trna Met f at neutral reaction. Phenylalanyl-tRNA, N-acetylphenylalanyl-tRNA and methionyl-tRNA Met m are not hydro...

journal_title：Nucleic acids research

authors： Hradec J

更新日期：1975-11-01 00:00:00

abstract：:Diastereomeric dithymidine methanephosphonamidates (TnpmT) were synthesized by reaction of 3'-amino-3'-deoxythymidine with 3'- O -acetylthymidin-5-yl-methanephosphonochloridate. Separated dinucleotide TnpmT(fast) and TnpmT(slow) diastereomers were used as building blocks to prepare chimeric dodecathy-midylates, posses...

journal_title：Nucleic acids research

authors： Nawrot B,Boczkowska M,Wójcik M,Sochacki M,Kazmierski S,Stec WJ

更新日期：1998-06-01 00:00:00

abstract：:NF-IL6beta regulates gene expression and plays function roles in many tissues. The EGF-regulated cyclooxygenase-2 (cox-2) expression is mediated through p38(MAPK) signaling pathway and positively correlates with NF-IL6beta expression in A431 cells. NF-IL6beta coordinated with c-Jun on cox-2 transcriptional activation ...

journal_title：Nucleic acids research

authors： Wang JM,Ko CY,Chen LC,Wang WL,Chang WC

更新日期：2006-01-05 00:00:00

abstract：:Aberration within the p53 tumor suppressor gene is the most frequently identified genetic damage in human cancer. Regulatory functions proposed for the p53 protein include modulation of the cell cycle, cellular differentiation, signal transduction, and gene expression. Additionally, the p53 gene product may guard the ...

journal_title：Nucleic acids research

authors： Hulla JE,Schneider RP

更新日期：1993-02-11 00:00:00

abstract：:Alterations in cancer genomes strongly influence clinical responses to treatment and in many instances are potent biomarkers for response to drugs. The Genomics of Drug Sensitivity in Cancer (GDSC) database (www.cancerRxgene.org) is the largest public resource for information on drug sensitivity in cancer cells and mo...

journal_title：Nucleic acids research

authors： Yang W,Soares J,Greninger P,Edelman EJ,Lightfoot H,Forbes S,Bindal N,Beare D,Smith JA,Thompson IR,Ramaswamy S,Futreal PA,Haber DA,Stratton MR,Benes C,McDermott U,Garnett MJ

更新日期：2013-01-01 00:00:00

abstract：:The synthetic DNA fragment (formula, see text) (corresponding to nucleotides 4299-4314 of the phi X DNA sequence) was cloned into either the AmpR gene or the KmR gene of plasmid pACYC 177. The DNA sequence of the KmR gene around the insertion site was determined by nucleotide sequence analysis of the pACYC 177 FnudII ...

journal_title：Nucleic acids research

authors： Heidekamp F,Baas PD,van Boom JH,Veeneman GH,Zipursky SL,Jansz HS

更新日期：1981-07-24 00:00:00

abstract：:Perturbation and time-course data sets, in combination with computational approaches, can be used to infer transcriptional regulatory networks which ultimately govern the developmental pathways and responses of cells. Here, we individually knocked down the four transcription factors PU.1, IRF8, MYB and SP1 in the huma...

journal_title：Nucleic acids research

authors： Vitezic M,Lassmann T,Forrest AR,Suzuki M,Tomaru Y,Kawai J,Carninci P,Suzuki H,Hayashizaki Y,Daub CO

更新日期：2010-12-01 00:00:00

abstract：:The triphosphate of the nucleoside deoxyribosyl dihydropyrimido[4,5-c][1,2]oxazin-7-one (dP) is known to be incorporated into DNA efficiently by Taq polymerase and is a useful tool for polymerase-mediated in vitro mutagenesis. It is shown here that dP is a potent mutagen in Escherichia coli and Salmonella typhimurium ...

journal_title：Nucleic acids research

authors： Negishi K,Williams DM,Inoue Y,Moriyama K,Brown DM,Hayatsu H

更新日期：1997-04-15 00:00:00

abstract：:One surprisingly common element of RNA secondary structure consists of a hairpin capped by a four-base loop (or the tetraloop). Recently the 3-D structures of two RNA-tetraloops have been determined by NMR-studies. Both structures have a similar architecture: the first and the last bases of the loop form a hydrogen bo...

journal_title：Nucleic acids research

authors： Kajava A,Rüterjans H

更新日期：1993-09-25 00:00:00

abstract：:DNA assembly allows individual DNA constructs or libraries to be assembled quickly and reliably. Most methods are either: (i) Modular, easily scalable and suitable for combinatorial assembly, but leave undesirable 'scar' sequences; or (ii) bespoke (non-modular), scarless but less suitable for construction of combinato...

journal_title：Nucleic acids research

authors： Taylor GM,Mordaka PM,Heap JT

更新日期：2019-02-20 00:00:00

abstract：:In Saccharomyces cerevisiae, the DNA damage response (DDR) is activated by the spatio-temporal colocalization of Mec1-Ddc2 kinase and the 9-1-1 clamp. In the absence of direct means to monitor Mec1 kinase activation in vivo, activation of the checkpoint kinase Rad53 has been taken as a proxy for DDR activation. Here, ...

journal_title：Nucleic acids research

authors： Janke R,Herzberg K,Rolfsmeier M,Mar J,Bashkirov VI,Haghnazari E,Cantin G,Yates JR 3rd,Heyer WD

更新日期：2010-04-01 00:00:00

abstract：:MetaNetX is a repository of genome-scale metabolic networks (GSMNs) and biochemical pathways from a number of major resources imported into a common namespace of chemical compounds, reactions, cellular compartments--namely MNXref--and proteins. The MetaNetX.org website (http://www.metanetx.org/) provides access to the...

journal_title：Nucleic acids research

authors： Moretti S,Martin O,Van Du Tran T,Bridge A,Morgat A,Pagni M

更新日期：2016-01-04 00:00:00

abstract：:Heteroduplexes between the viral DNA of phiX174 and DNA from the replicative form (RF) of phage G4 were examined by electron microscopy. The single Eco RI site of G4-RF was utilized as a physical marker by preparing the heteroduplexes from the denatured, linear DNA obtained by restricting G4-RF with Eco RI endonucleas...

journal_title：Nucleic acids research

authors： Smiley BL,Warner RC

更新日期：1979-01-01 00:00:00

abstract：:Transcript of the rbpA1 gene in Anabaena variabilis accumulates significantly at low growth temperatures below 28 degreesC. This accumulation was maximal at 16 degreesC. Accumulation of the rbpA1 transcript was completely abolished by rifampicin, but not by chloramphenicol. Photosynthesis was not required for this col...

journal_title：Nucleic acids research

authors： Sato N,Nakamura A

更新日期：1998-05-01 00:00:00

abstract：:A new method for evaluating some complex characteristics of the primary structure of E.coli promoters is proposed. The method, of nonparametric statistical significance, selects important conserved single-base positions in combination with 2-base coupling relations of identity and complementarity. The extended consens...

journal_title：Nucleic acids research

authors： Rozkot F,Sázelová P,Pivec L

更新日期：1989-06-26 00:00:00

abstract：:DNA lengths in the structural repeat units of Chinese hamster ovary (CHO) and chicken erythrocyte chromatin were compared by analyzing the sizes of DNA fragments produced after treatment of nuclei with staphylococcal nuclease. The repeat length of CHO chromatin (173 +- 4 BP) is about 20 base pairs (BP) smaller than th...

journal_title：Nucleic acids research

authors： Rill RL,Nelson DA,Oosterhof DK,Hozier JC

更新日期：1977-04-01 00:00:00

abstract：:The interaction of the high mobility group proteins, HMG14 and HMG17, with nucleosome core particles has been studied. The results show that two molecules of HMG14/17 can be bound tightly but reversibly to each core particle and that their affinity for core particles is greater than their affinity for histone-free DNA...

journal_title：Nucleic acids research

authors： Sandeen G,Wood WI,Felsenfeld G

更新日期：1980-09-11 00:00:00

abstract：:In order to direct antisense oligonucleotides to specific tissues or cell types in vivo, we are exploring the possibility to utilize lipoproteins as transport vehicles. A 16-mer oligonucleotide (ODN) was derivatized at the 5' prime through a 32P phosphate spacer with cholesterol, yielding a 32P-labeled amphiphatic cho...

journal_title：Nucleic acids research

authors： de Smidt PC,Le Doan T,de Falco S,van Berkel TJ

更新日期：1991-09-11 00:00:00

abstract：:Biological networks are a topic of great current interest, particularly with the publication of a number of large genome-wide interaction datasets. They are globally characterized by a variety of graph-theoretic statistics, such as the degree distribution, clustering coefficient, characteristic path length and diamete...

journal_title：Nucleic acids research

authors： Yu H,Zhu X,Greenbaum D,Karro J,Gerstein M

更新日期：2004-01-14 00:00:00

abstract：:The widely accepted model for the processing of tRNAs in Escherichia coli involves essential initial cleavages by RNase E within polycistronic transcripts to generate pre-tRNAs that subsequently become substrates for RNase P. However, recently we identified two polycistronic tRNA transcripts whose endonucleolytic proc...

journal_title：Nucleic acids research

authors： Mohanty BK,Kushner SR

更新日期：2008-02-01 00:00:00

abstract：:Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression tha...

journal_title：Nucleic acids research

authors： Kubo M,Nishiyama T,Tamada Y,Sano R,Ishikawa M,Murata T,Imai A,Lang D,Demura T,Reski R,Hasebe M

更新日期：2019-05-21 00:00:00

abstract：:The nucleotide excision repair pathway removes ultraviolet (UV) photoproducts from the human genome in the form of short oligonucleotides ∼ 30 nt in length. Because there are limitations to many of the currently available methods for investigating UV photoproduct repair in vivo, we developed a convenient non-radioisot...

journal_title：Nucleic acids research

authors： Choi JH,Gaddameedhi S,Kim SY,Hu J,Kemp MG,Sancar A

更新日期：2014-02-01 00:00:00

abstract：:We have isolated a human cDNA (kup), encoding a new protein with two distantly spaced zinc fingers of the C2H2 type. This gene is highly conserved in mammals and is expressed mainly in hematopoietic cells and testis. Its expression was not higher in the various transformed cells tested than in the normal corresponding...

journal_title：Nucleic acids research

authors： Chardin P,Courtois G,Mattei MG,Gisselbrecht S

更新日期：1991-04-11 00:00:00

abstract：:The nucleotide sequence of Drosophila melanogaster tRNA 5 Lys is pGCCCGGAUAm2GCUCAGDCGGDAGAGCA psi psi GGACUsU*UUt6A*A psi CCAAGGm7GDm5CCAGGGTm psi CAm1AGUCCCUGUUCGGGCGCCA. The sU* is probably 5-methylcarboxymethyl-2-thiouridine and t6A* is a mixture of modified derivatives of t6A including t6A itself and a component ...

journal_title：Nucleic acids research

authors： Cribbs DL,Gillam IC,Tener GM

更新日期：1982-10-25 00:00:00