Abstract:
:Impaired lung epithelial Na(+) channel (ENaC) activity at the time of birth results in respiratory distress. To investigate potential mechanisms, the ontogeny and cellular distribution of the alphaENaC subunit mRNA expression was studied in normal, immature, and abnormal (hypoplastic) human fetal lungs using nonradioisotopic in situ hybridization. Surprisingly, alphaENaC expression was detected at the embryonic stage of normal lung development (4 to 5 wk gestation) when expression was localized to the fetal lung bud epithelium. By late gestation, ENaC was expressed in the conductive and respiratory airway epithelium, serous cells, and the distal lung unit in an alveolar type II (ATII) epitheliumlike distribution. Significant alphaENaC expression was found in newborn lung diseases associated with respiratory distress. One explanation is that alphaENaC mRNA is constitutively expressed, and that activity is regulated, at least in part, at the post-transcriptional level. Alternative explanations are that the expression of the beta or gammaENaC subunits may be impaired in certain newborn lung diseases or that alternate Na(+) permeant channels or transporters are important to lung liquid absorption in humans at birth.
journal_name
Am J Respir Crit Care Medauthors
Smith DE,Otulakowski G,Yeger H,Post M,Cutz E,O'Brodovich HMdoi
10.1164/ajrccm.161.4.9905064keywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
1322-31issue
4 Pt 1eissn
1073-449Xissn
1535-4970journal_volume
161pub_type
杂志文章abstract:RATIONALE:No methodical assessment of the lung, cardiac, and sleep function of patients surviving an acute hypercapnic respiratory failure episode requiring admission to the intensive care unit (ICU) has been reported in the literature. OBJECTIVES:To prospectively investigate the prevalence and impact of comorbidities...
journal_title:American journal of respiratory and critical care medicine
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更新日期:2017-07-15 00:00:00
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更新日期:2020-12-11 00:00:00
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journal_title:American journal of respiratory and critical care medicine
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更新日期:2010-02-15 00:00:00
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pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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更新日期:2015-10-01 00:00:00
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更新日期:2016-08-15 00:00:00
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doi:10.1164/ajrccm.154.6.8970360
更新日期:1996-12-01 00:00:00
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更新日期:2021-02-01 00:00:00
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更新日期:2005-07-01 00:00:00
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更新日期:1996-07-01 00:00:00
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更新日期:2002-04-01 00:00:00
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更新日期:2015-07-01 00:00:00
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更新日期:2017-09-01 00:00:00
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更新日期:2014-06-01 00:00:00
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更新日期:2013-08-01 00:00:00
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更新日期:2008-11-01 00:00:00