Heat-induced apoptosis in human prostatic stromal cells.

Abstract:

OBJECTIVE:To determine whether heat, used in transurethral microwave thermotherapy (TUMT) for benign prostatic hyperplasia and which causes necrotic lesions within the adenoma, induces apoptosis in benign human prostatic stromal cells. Materials and methods Prostatic stromal cells were cultured from benign human prostatic tissue. The origin of the cells was identified by immunohistochemical staining and transmission electron microscopy. Cell cultures were exposed to moderate hyperthermia (47 degrees C) for 1 h and any apoptosis detected by light microscopy, transmission electron microscopy and the measurement of induced caspase-3-like activity. RESULTS:The cultures contained a mixed population of smooth muscle cells and myofibroblasts. Twenty-four hours after heat exposure, 76% of the cells were apoptotic and the caspase activity had increased, whereas only 14% of the cells were necrotic. CONCLUSION:Moderate hyperthermia induces apoptosis in cultured human prostatic stromal cells.

journal_name

BJU Int

journal_title

BJU international

authors

Brehmer M,Svensson I

doi

10.1046/j.1464-410x.2000.00473.x

keywords:

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

535-41

issue

4

eissn

1464-4096

issn

1464-410X

pii

bju473

journal_volume

85

pub_type

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