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Establishment of a testicular carcinoma cell line producing alpha-fetoprotein.

Abstract：

OBJECTIVE:To characterize a newly established human testicular carcinoma cell line that continuously produces alpha-fetoprotein (AFP), and to investigate the effects of retinoic acid on AFP production. MATERIALS AND METHODS:A 24-year-old man underwent a radical orchidectomy for a right testicular tumour and was found to have two separate metastatic lesions in the lungs, both of which were removed surgically. The cancer cells were isolated from one of the tumours, which was composed of undifferentiated germ cells and produced AFP; the cells were cultured in a monolayer. This cell line was designated as KU-MT. RESULTS:The cell line was successfully maintained both in athymic nude mice and in culture. Histological examination showed that the xenografted tumours were composed of cells in the reticular, solid and glandular patterns of a yolk sac tumour, and of embryonal carcinoma cells. These cells immunostained positively for AFP. On electron microscopy, the extracellular deposition of a basement lamina-like substance, a typical feature of yolk sac tumour, was detected. The AFP production in mice correlated well with the tumour weight of the xenograft. The cultured KU-MT cells were oval to polygonal in morphology and grew exponentially, with a population doubling time of approximately 2 days. Chromosomal analysis showed a modal number of 57 with consistent structural abnormalities of +add(1)(p13), del(1)(q32), del(2)(q31), add(6) (q21), +add(9)(p22), add(11)(p15), and add(14)(p11). Reverse-transcription polymerase chain reaction analysis showed that the retinoic acid receptors (RAR)-alpha, RAR-gamma, and retinoid X receptor-alpha were present in the cells. The expression of AFP mRNA was up-regulated in response to all-trans-retinoic acid; treatment with this agent caused morphological changes and induced apoptosis in the cells. CONCLUSIONS:This newly established cell line provides a reproducible model system that should offer a good insight into the differentiation of testicular carcinoma.

journal_name

BJU Int

journal_title

BJU international

authors

Ueno M,Nakashima J,Ohigashi T,Deguchi N,Ban S,Akita M,Murai M

doi

10.1046/j.1464-410x.2001.02357.x

keywords:

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

611-21

issue

eissn

1464-4096

issn

1464-410X

pii

2357

journal_volume

pub_type

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Establishment of a testicular carcinoma cell line producing alpha-fetoprotein.