Abstract：
:The COPI coatomer is thought to be required in yeast directly for retrograde transport from the Golgi to the endoplasmic reticulum (ER), and directly or indirectly for ER-to-Golgi transport. Unexpectedly, the secretory glycoproteins Hsp150 and invertase have been found not to require COPI for ER exit. The features according to which cargo proteins are selected for the COPI-independent pathway are not known. The ER form of Hsp150 has three distinct domains: an N-terminal fragment of 54 amino acids (subunit I) is followed by 11 repeats of a 19 amino acid peptide plus a unique C-terminal fragment of 114 amino acids (subunit II). By fusing heterologous proteins to different Hsp150 domains and expressing them in sec21-1 and sec21-3 mutants with temperature-sensitive mutations in the gamma-COPI subunit, we show here that the repeats of subunit II function as sorting determinants for COPI-independent ER exit. The C-terminal fragment of Hsp150 could be replaced by E. coli beta-lactamase or rat nerve growth factor receptor ectodomain (NGFRe), and subunit I could be deleted, without inhibiting COPI-independent transport. However, when the repetitive region was omitted and beta-lactamase was fused directly to the C terminus of subunit I, COPI was required for efficient ER exit. Mass spectroscopic analysis demonstrated that both subunit I and II of Hsp150 were extensively O-glycosylated, suggesting that the O-glycosylation pattern was not decisive for cargo selection.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Suntio T,Shmelev A,Lund M,Makarow Mkeywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
3889-98eissn
0021-9533issn
1477-9137journal_volume
112 ( Pt 22)pub_type
杂志文章abstract：:Dyneins are motor proteins that move along microtubules. They have many roles in the cell. They drive the beating of cilia and flagella, move cargos in the cytoplasm and function in the mitotic spindle. Dyneins are large and complex protein machines. Until recently, the way they move was poorly understood. In 2012, tw...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.120725
更新日期：2013-02-01 00:00:00
abstract：:Cyclin E is a regulatory subunit of the cdc2-related protein kinase cdk2, which is activated shortly before S-phase entry, thus defining it as a G1 cyclin. We report here the existence of a 43 kDa splice variant of human cyclin E, termed cyclin Es, which lacks 49 amino acids within the cyclin box compared to the known...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:The dynamics of cell adhesion sites control cell morphology and motility. Adhesion-site turnover is thought to depend on the local availability of the acidic phospholipid phosphatidylinositol-4,5-bisphosphate (PIP(2)). PIP(2) can bind to many cell adhesion proteins such as vinculin and talin, but the consequences of t...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01734
更新日期：2005-04-01 00:00:00
abstract：:Actomyosin stress fibers (SFs) enable cells to exert traction on planar extracellular matrices (ECMs) by tensing focal adhesions (FAs) at the cell-ECM interface. Although it is widely appreciated that the spatial and temporal distribution of these tensile forces play key roles in polarity, motility, fate choice, and o...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.119032
更新日期：2013-07-15 00:00:00
abstract：:The chromosomes of the active previtellogenic oocytes of the dragonfly Cordulia aenea L. extrude into the cytoplasm a substance, which is subsequently found there in the form of granulofibrillar masses. These nuclear extrusions evolve ultrastructurally into two components, nematosomes and dense masses. The nematosomes...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1975-10-01 00:00:00
abstract：:CK2 is a serine/threonine kinase with many substrates, largely unknown modes of regulation and essential roles in mitotic progression. CK2α, a catalytic subunit of CK2, is phosphorylated in mitosis, and here we examine the effect of phosphorylation on CK2α localization. Using phosphospecific antibodies, we show that C...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.077446
更新日期：2011-07-15 00:00:00
abstract：:Myosin-X (Myo10) is an unconventional myosin with MyTH4-FERM domains that is best known for its striking localization to the tips of filopodia and its ability to induce filopodia. Although the head domain of Myo10 enables it to function as an actin-based motor, its tail contains binding sites for several molecules wit...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.023549
更新日期：2011-11-15 00:00:00
abstract：:The influence of extracellular environmental factors on preimplantation rat blastocysts was tested by determining the number of embryos which escaped from their zonae pellucidae, followed by attachment and outgrowth of trophoblast giant cells, after 72 h in culture Uterine luminal ocmponents from individual females, o...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:Small latent TGF-beta consists of latency associated peptide (LAP) bound to the 25 kDa TGF-beta by noncovalent interactions. Small latent TGF-beta is secreted from cells and deposited into the extracellular matrix as covalent complexes with its binding proteins, LTBPs. Four LTBPs have been molecularly cloned and their...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
abstract：:Lysosomal cysteine proteases from mammalian cells and plants are regulated by endogenous tight-binding inhibitors from the cystatin superfamily. The presence of cystatin-like inhibitors in lower eukaryotes such as protozoan parasites has not yet been demonstrated, although these cells express large quantities of cyste...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:We use the fungus Ashbya gossypii to investigate how its polar growth machinery is organized to achieve sustained hyphal growth. In slowly elongating hyphae exocyst, cell polarity and polarisome proteins permanently localize as cortical cap at hyphal tips, thus defining the zone of secretory vesicle fusion. In tenfold...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.033852
更新日期：2008-12-01 00:00:00
abstract：:A universal feature of mitosis is that all chromosomes become aligned at the spindle equator--the halfway point between the two spindle poles--prior to anaphase onset. This migratory event is called congression, and is powered by centromere-bound protein machines called kinetochores. This Commentary aims to document r...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.169367
更新日期：2015-09-15 00:00:00
abstract：:The biosynthetic machinery in the melanotrope cells of the Xenopus intermediate pituitary is primarily dedicated to the generation of proopiomelanocortin (POMC)-derived, melanophore-stimulating peptides. Transfer of the animal to a black background stimulates the production of these peptides and causes a dramatic incr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:Ryanodine receptor subtype 1 (RyR1) has been primarily characterized in skeletal muscle but several studies have revealed its expression in smooth muscle. Here, we used Ryr1-null mice to investigate the role of this isoform in Ca(2+) signaling in urinary bladder smooth muscle. We show that RyR1 is required for depolar...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.009415
更新日期：2007-11-01 00:00:00
abstract：:The function of DAXX, a highly conserved mammalian gene, has remained controversial; this is due, in part, to its identification in a variety of yeast two-hybrid screens. Targeted deletion in the mouse revealed that DAXX is essential for embryonic development. Furthermore, the increased levels of apoptosis observed in...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00234
更新日期：2003-01-15 00:00:00
abstract：:Angiogenesis is critical for vascular remodeling during development and contributes to the pathogenesis of diseases such as cancer. Targeted disruption of several EphB class receptor tyrosine kinases results in vascular remodeling defects during embryogenesis. The role of EphA class receptors in vascular remodeling, h...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01061
更新日期：2004-04-15 00:00:00
abstract：:Intermediate filament protein keratin 8 (K8) binds to heat shock protein 70 (Hsp70) and p38 MAPK, and is phosphorylated at Ser74 by p38α (MAPK14, hereafter p38). However, a p38 binding site on K8 and the molecular mechanism of K8-p38 interaction related to Hsp70 are unknown. Here, we identify a p38 docking site on K8 ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.229534
更新日期：2019-09-26 00:00:00
abstract：:Human SW-13 cells express the intermediate filament protein vimentin in a mosaic pattern (Hedberg, K. K. and Chen, L. B. (1986). Exp. Cell Res. 163, 509-517). We have isolated SW-13 clones that do (vim+) or do not (vim-) synthesize vimentin as analyzed using anti-intermediate filament immunofluorescence, electron micr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Using a polyclonal antibody against purified zymogen granule membrane components from rat pancreas a cDNA coding for the 29 kDa protein (ZG29p) was identified by immunoscreening of a hormonally stimulated pancreas cDNA library. Western blot analysis suggests that ZG29p is a pancreas-specific protein and immunofluoresc...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:Homophilic binding of E-cadherins through their ectodomains is fundamental to epithelial cell-cell adhesion. Despite this, E-cadherin ectodomains have evolved differently in the vertebrate and insect lineages. Of the five rod-like, tandemly aligned extracellular cadherin domains of vertebrate E-cadherin, the tip extra...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.189258
更新日期：2016-09-01 00:00:00
abstract：:Keratins 8 and 18 (collectively referred to as K8/K18) are the major components of intermediate filaments of simple epithelial cells. Recent studies have revealed the function of K8/K18 in apoptosis modulation. Here, we show that eIF3k, originally identified as the smallest subunit of eukaryotic translation initiation...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.021394
更新日期：2008-07-15 00:00:00
abstract：:Compartmentalization of membrane transport and signaling processes is of pivotal importance to eukaryotic cell function. While plasma membrane compartmentalization and dynamics are well known to depend on the scaffolding function of septin GTPases, the roles of septins at intracellular membranes have remained largely ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.225557
更新日期：2019-02-01 00:00:00
abstract：:We first identified Bves (blood vessel/epicardial substance) as a transmembrane protein that localized to the lateral compartment of the epithelial epicardium. Bves traffics to sites of cell-cell contact in cultured epicardial cells and promotes adhesion following transfection into non-adherent fibroblastic L-cells, r...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02588
更新日期：2005-10-15 00:00:00
abstract：:Inactivation of the transcription factor and tumor suppressor p53, and overexpression or mutational activation of PIK3CA, which encodes the p110alpha catalytic subunit of phosphatidylinositol-3-kinase (PI3K), are two of the most common deleterious genomic changes in cancer, including in ovarian carcinomas. We investig...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.013029
更新日期：2008-03-01 00:00:00
abstract：:Feedback mechanisms preventing M phase occurrence before S phase completion are assumed to depend on inhibition of cyclin B-cdc2 kinase activation by unreplicated DNA. In sea urchin, fertilization stimulates protein synthesis and releases eggs from G1 arrest. We found that in the one-cell sea urchin embryo cyclin B-cd...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:In somatic cells, the heterodimeric Structural Maintenance of Chromosomes (SMC) proteins are involved in chromosome condensation and gene dosage compensation (SMC2 and 4), and sister chromatid cohesion and DNA recombination (SMC1 and 3). We report here evidence for an involvement of mammalian SMC1 and SMC3 proteins in...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.058693
更新日期：2010-01-15 00:00:00
abstract：:A fundamental prediction of the hypothesis that critical events in tumour formation are rare is that the tumours develop by clonal expansion. This prediction has been tested by establishing the isozyme content of tumours induced in mosaic animals whose normal tissues contain two distinguishable isozyme activities. The...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:Wound healing crucially relies on the mechanical activity of fibroblasts responding to TGFβ1 and to forces transmitted across focal adhesions. Integrin-linked kinase (ILK) is a central adapter recruited to integrin β1 tails in focal adhesions mediating the communication between cells and extracellular matrix. Here, we...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.063024
更新日期：2010-11-15 00:00:00
abstract：:Members of the PKC superfamily have been implicated in various migratory models and in particular in spatially restricted processes. However, defining the precise local events that underlie the PKC-dependent processes is constrained by the unspecific nature of interventions. Here we address this problem in relation to...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.062299
更新日期：2010-08-15 00:00:00
