Analysis of myelodysplastic syndrome clones arising after multiple myeloma: a case study by correlative interphase cytogenetic analysis.

Abstract:

BACKGROUND:A patient with multiple myeloma developed myelodysplastic syndrome (MDS). Chromosomal analysis performed after the development of MDS revealed monosomy of chromosome 9 in all the meta-phases. We wished to identify the extent of the clone with the chromosomal abnormality originating from MDS clone. METHODS:A correlative interphase study by fluorescence in situ hybridization (FISH) was performed and we determined whether each lineage of cells obtained the molecular mark. The chromosome 9 classic alpha satellite region DNA was used as a probe for the FISH analysis in smear specimens stained with Wright-Giemsa stain. RESULTS:Erythroblasts, granulocytes and myelocytes had only one signal, whereas myeloma cells showed two to four signals. CONCLUSION:This study visualized the spectrum of MDS clone. The results suggest that the origin of MDS is different from that of multiple myeloma, at least in this case.

journal_name

Jpn J Clin Oncol

authors

Kobayashi Y,Nakayama M,Uemura N,Takeyama K,Tobinai K,Takenaka T,Choi SH,Satoh H,Mori S,Asano S

doi

10.1093/jjco/29.8.374

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

374-7

issue

8

eissn

0368-2811

issn

1465-3621

journal_volume

29

pub_type

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