当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF ONCOLOGY期刊下所有文献 > Activation of RB tumor suppressor protein and growth suppression of small cell lung carcinoma cells by reintroduction of p16INK4A gene.

Activation of RB tumor suppressor protein and growth suppression of small cell lung carcinoma cells by reintroduction of p16INK4A gene.

Abstract：

:The p16INK4A tumor suppressor gene is frequently inactivated in non-small cell lung carcinoma (NSCLC) and is less frequently inactivated in small cell lung carcinoma (SCLC) by mutation, deletion or DNA methylation. There are several reports that the reintroduction of the p16INK4A gene into p16(-) NSCLC cells results in significant growth suppression. However, there have been no reports of reintroduction of the p16INK4A gene into SCLC cells. To assess the biological significance of p16INK4A inactivation in the development of SCLC, full-length p16INK4A cDNA was introduced into an SCLC cell line, Ms-13, in which the p16INK4A protein was not detected. SCLC cells stably transfected with the p16INK4A expression vector formed only 2-16% of the number of neomycin-resistant colonies formed by cells transfected with a control vector, and no expression of exogenous p16INK4A protein was detected in any of 16 expanded clones. Transient transfection of the p16INK4A gene into SCLC cells resulted in exogeneous p16INK4A protein expression and dephosphorylation of endogenous retinoblastoma (RB) protein. These results suggest that the restoration of the p16INK4A function suppresses the growth of SCLC cells by dephosphorylation of the RB protein. Therefore, inactivation of p16INK4A may play an important role in the enhancement of growth of p16INK4A(-) RB(+) SCLC tumors in vivo.

journal_name

Int J Oncol

journal_title

International journal of oncology

authors

Sumitomo K,Shimizu E,Shinohara A,Yokota J,Sone S

doi

10.3892/ijo.14.6.1075

keywords:

subject

Has Abstract

pub_date

1999-06-01 00:00:00

pages

1075-80

issue

6

eissn

1019-6439

issn

1791-2423

journal_volume

14

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF ONCOLOGY文献大全
  • Alphavirus vectors for cancer gene therapy (review).

    abstract：:Alphaviruses have several characteristics that make them attractive as gene therapy vectors such as transient and high-level expression of a heterologous gene. Alphavirus vectors, Semliki Forest virus (SFV), Sindbis virus (SIN) and Venezuelan equine encephalitis virus (VEE) have been developed as gene expression vecto...

    journal_title：International journal of oncology

    pub_type： 杂志文章,评审

    doi：

    authors： Yamanaka R

    更新日期：2004-04-01 00:00:00

  • A model of spontaneous mouse mammary tumor for human estrogen receptor- and progesterone receptor-negative breast cancer.

    abstract：:Breast cancer (BC) is the most frequently malignancy in women. Therefore, establishment of an animal model for the development of preventative measures and effective treatment for tumors is required. A novel heterogeneous spontaneous mammary tumor animal model of Kunming mice was generated. The purpose of this study w...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.2014.2657

    authors： Zheng L,Zhou B,Meng X,Zhu W,Zuo A,Wang X,Jiang R,Yu S

    更新日期：2014-12-01 00:00:00

  • Prognostic significance of cyclin expression in human osteosarcoma.

    abstract：:To evaluate the distribution of cyclin protein expression, in relation to cell proliferation rate and clinical behavior, an immunohistochemical study was performed on 92 tumor samples of patients with high grade osteosarcoma (OS). A large cyclin A- and cyclin E-positive fraction was found respectively in 59% and 47% o...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：

    authors： Molendini L,Benassi MS,Magagnoli G,Merli M,Sollazzo MR,Ragazzini P,Gamberi G,Ferrari C,Balladelli A,Bacchini P,Picci P

    更新日期：1998-05-01 00:00:00

  • Functional characterization of AIBp, a novel Aurora-A binding protein in centrosome structure and spindle formation.

    abstract：:Aurora-A is involved in chromosome alignment, centrosome maturation, mitotic spindle assembly and regards to an oncogene. Aurora-A is also known to bind to several other proteins affecting its up-regulation or down-regulation and localization. However, how these different binding signals work together to regulate Auro...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo_00000691

    authors： Lieu AS,Cheng TS,Chou CH,Wu CH,Hsu CY,Huang CY,Chang LK,Loh JK,Chang CS,Hsu CM,Howng SL,Hong YR

    更新日期：2010-08-01 00:00:00

  • Polymorphisms in the glutathione S-transferase mu cluster are associated with tumour progression and patient outcome in colorectal cancer.

    abstract：:Glutathione S-transferase (GST) enzymes catalyse the detoxification of by-products of reactive oxygen species and are thus important in cellular defence mechanisms. The GSTs are polymorphic with allelic variants encoding isoforms with functional differences. GST polymorphism has been associated with susceptibility and...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：

    authors： Holley SL,Rajagopal R,Hoban PR,Deakin M,Fawole AS,Elder JB,Elder J,Smith V,Strange RC,Fryer AA

    更新日期：2006-01-01 00:00:00

  • Suppression of prostate tumor cell growth in vivo by WT1, the Wilms' tumor suppressor gene.

    abstract：:The primary form of therapy for prostate cancer is androgen ablation resulting in apoptosis and expression of apoptotic genes (i.e. par-4). Prostate cancer cells that survive androgen ablation therapy express pro-survival genes (i.e. bcl-2) permitting these androgen independent (AI) cells to overcome apoptotic signals...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：

    authors： Fraizer G,Leahy R,Priyadarshini S,Graham K,Delacerda J,Diaz M

    更新日期：2004-03-01 00:00:00

  • Tyrosine receptor kinase B silencing inhibits anoikis‑resistance and improves anticancer efficiency of sorafenib in human renal cancer cells.

    abstract：:Renal cell carcinoma (RCC) is the most common solid neoplasm of adult kidney, and the major treatment for metastatic RCC (mRCC) is molecular targeted therapy. Sorafenib, as a multi-targeted tyrosine kinase inhibitor (TKI), has significantly improved clinical outcomes of mRCC patients. However, complete or long-term re...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.2016.3356

    authors： Zhang P,Xing Z,Li X,Song Y,Zhao J,Xiao Y,Xing Y

    更新日期：2016-04-01 00:00:00

  • ONO-4007, a synthetic lipid A analog, induces Th1-type immune response in tumor eradication and restores nitric oxide production by peritoneal macrophages.

    abstract：:We have reported that ONO-4007, a novel synthetic lipid A derivative with low toxic activities, produced large amount of tumor necrosis factor (TNF)-alpha selectively in the tumor tissues and brought about complete cures in about 60% of rats bearing TNF-alpha sensitive KDH-8 cells, which also secreted a large amount o...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：

    authors： Matsushita K,Kuramitsu Y,Ohiro Y,Kobayashi I,Watanabe T,Kobayashi M,Hosokawa M

    更新日期：2003-08-01 00:00:00

  • Characterization of a h-3 GnRH method for the measurement of GnRH binding-sites in human breast-cancer and breast-cancer cell-lines.

    abstract：:The peptide hormone, GnRH has been hypothesized to play a direct antitumoral role in certain kinds of breast cancer. This direct effect is considered to function via high affinity membrane receptors to GnRH that have been demonstrated in tumors. However, no routine assay for GnRH receptors exist limiting further resea...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：

    authors： Reshkin S,Pezzetta A,Albarani V,Schittulli F,Paradiso A

    更新日期：1995-08-01 00:00:00

  • Paracrine interactions of vascular endothelial growth factor and platelet-derived growth factor in endothelial and lung cancer cells.

    abstract：:While the effects of single growth factors on endothelial cells (ECs) have been extensively studied, the importance of induction of growth factors such as PDGF-BB (platelet derived growth factor) in ECs and its impact on tumor cell functions are only partly understood. Human umbilical vein endothelial cells (HUVECs) w...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：

    authors： Reinmuth N,Rensinghoff S,Raedel M,Fehrmann N,Schwöppe C,Kessler T,Bisping G,Hilberg F,Roth GJ,Berdel W,Thomas M,Mesters RM

    更新日期：2007-09-01 00:00:00

  • Preoperative radiotherapy for muscle-invading bladder-carcinoma.

    abstract：:One hundred five patients with clinical stage T2-T4 transitional cell bladder carcinoma who completed either low-dose (20-26 Gy) or high dose (40-50 Gy) preoperative radiotherapy (RT) followed by cystectomy were reviewed. Five-year actuarial survival was 42%, 52%, and 17% for stages T2, T3, and T4, respectively. Five-...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.1.5.555

    authors： Cox J,Greven K,Anscher M,Morgan T

    更新日期：1992-10-01 00:00:00

  • Therapeutic results of intra-arterial chemotherapy in patients with malignant glioma.

    abstract：:Thirty-three adult patients with anaplastic astrocytoma or glioblastoma multiforme, verified between 1990 and 1997, received postoperative intra-arterial ACNU chemotherapy and irradiation. Since our previous strategies to malignant gliomas had included intra-venous ACNU, the purpose of the present study is to evaluate...

    journal_title：International journal of oncology

    pub_type： 临床试验,杂志文章

    doi：10.3892/ijo.13.3.537

    authors： Hirano Y,Mineura K,Mizoi K,Tomura N

    更新日期：1998-09-01 00:00:00

  • Establishment and characterization of GCSR1, a multi-drug resistant signet ring cell gastric cancer cell line.

    abstract：:Signet ring cell gastric cancer (SRCGC) has very poor prognosis worldwide, and studying its molecular characteristics is urgent for improving the outcome. However, few well-characterized SRCGC cell lines are available for research. Therefore, we established a novel cell line GCSR1, from a Chinese male SRCGC patient. C...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.2015.2966

    authors： Xu X,Qian LJ,Su XY,He KF,Jin KT,Gu LH,Feng JG,Li GL,Zhou Q,Xu ZZ,Wang HH,Zhang J,Cao J,Teng LS

    更新日期：2015-01-01 00:00:00

  • Elevated levels of cathepsin B in human glioblastoma cell lines.

    abstract：:Degradation of the extracellular matrix is a prerequisite for the invasive phenotype in glioma cells. Several proteases released by invading tumor cells seem to participate in the focal degradation of extracellular matrix proteins. Using enzymatic assays, Western blotting, and Northern blotting techniques, we investig...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.19.3.519

    authors： Konduri S,Lakka SS,Tasiou A,Yanamandra N,Gondi CS,Dinh DH,Olivero WC,Gujrati M,Rao JS

    更新日期：2001-09-01 00:00:00

  • [Corrigendum] miR-28-5p promotes the development and progression of ovarian cancer through inhibition of N4BP1.

    abstract：:Following the publication of this article, the authors noted that there was an error in the affiliations. Specifically, the affiliation of the first author JUAN XU should be: Jinhua Municipal Central Hospital, Jinhua Hospital of Zhejiang University, Jinhua, Zhejiang 321000, P.R. China. [the original article was publis...

    journal_title：International journal of oncology

    pub_type： 杂志文章,已发布勘误

    doi：10.3892/ijo.2017.3977

    authors： Xu J,Jiang N,Shi H,Zhao S,Yao S,Shen H

    更新日期：2017-06-01 00:00:00

  • A novel cromakalim analogue induces cell cycle arrest and apoptosis in human cervical carcinoma HeLa cells through the caspase- and mitochondria-dependent pathway.

    abstract：:In the present study, a series of seven synthetic croma-kalim analogues were prepared and evaluated for cytotoxic effect on human cervical carcinoma HeLa cells using WST-8 assay. A preliminary screening of these cromakalim analogues showed that 1-[(3S,4R)-4-(2-ethoxy-4-methyl-1H-pyrrol-1-yl)-3-hydroxy- 2,2-dimethylchr...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.2011.1153

    authors： Zhang X,Zhao J,Kang S,Yi M,You S,Shin DS,Kim DK

    更新日期：2011-12-01 00:00:00

  • MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway.

    abstract：:Mammalian sterile-20-like kinase 4 (MST4) has been implicated in cell proliferation and differentiation. In a previous study, we found MST4 to be an important candidate gene for metastatic hepatocellular carcinoma (HCC); however, the molecular mechanism of the promoting role of MST4 in HCC metastasis is poorly underst...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.2014.2455

    authors： Lin ZH,Wang L,Zhang JB,Liu Y,Li XQ,Guo L,Zhang B,Zhu WW,Ye QH

    更新日期：2014-08-01 00:00:00

  • Induction of differentiation and tetraploidy by long-term treatment of C6 rat glioma cells with erucylphosphocholine.

    abstract：:Induction of differentiation represents a promising concept for chemotherapy of malignant gliomas, which are often refractory even to the combined treatment with surgery, irradiation and chemotherapy. Since anti-neoplastic alkylphosphocholines can induce differentiation of leukemic cell lines, the effects of the intra...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.19.4.673

    authors： Jendrossek V,Kugler W,Erdlenbruch B,Eibl H,Lakomek M

    更新日期：2001-10-01 00:00:00

  • The uptake of a monoclonal antibody (Po66) in lung tumour cell aggregates involves a phagocytose-like phenomenon.

    abstract：:The efficacy of radioimmunotherapy with radiolabelled monoclonal antibody (Mab) depends on the amount of antibody taken up by the tumour and on its intratumoral distribution. Multicellular spheroids (MTS) of lung cell carcinoma was investigated to study cellular and subcellular distribution of a Mab Po66 labelled with...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.10.3.609

    authors： Dazord L,Thomas D,Brichory F,Cavalier A,Meritte H,Desrues B,Cauletmaugendre S,Bourguet P

    更新日期：1997-03-01 00:00:00

  • Tumor oxygenation and radiosensitization by pentoxifylline and a perflubron emulsion carbogen breathing.

    abstract：:Oxygen tension measurements were made in three tumors: (i) the murine FSaII fibrosarcoma, (ii) the rat 9L gliosarcoma and (iii) the rat 13672 mammary adenocarcinoma using a pO2 histograph. Tumor oxygenation measurements were made while the animals breathed air or breathed carbogen (95% oxygen/5% carbon dioxide). Pento...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.2.1.13

    authors： Teicher B,Sotomayor E,Robinson M,Dupuis N,Schwartz G,Frei E

    更新日期：1993-01-01 00:00:00

  • Diosmetin suppresses human prostate cancer cell proliferation through the induction of apoptosis and cell cycle arrest.

    abstract：:Diosmetin, a plant flavonoid, has been shown to exert promising effects on prostate cancer cells as an anti‑proliferative and anticancer agent. In this study, using western blot analysis for protein expression and flow cytometry for cell cycle analysis, we determined that the treatment of the LNCaP and PC‑3 prostate c...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.2018.4407

    authors： Oak C,Khalifa AO,Isali I,Bhaskaran N,Walker E,Shukla S

    更新日期：2018-08-01 00:00:00

  • Mutations of the STK11 gene in sporadic gastric carcinoma.

    abstract：:Gastric carcinoma may occur sporadically or in association with hereditary diseases, such as Peutz-Jehgers syndrome (PJS). The PJS gene (named STK11 or LKB1) was mapped to 19p13.3 and recently cloned. Germ-line mutations of the gene have been detected in familial PJS patients and are predicted to predispose STK11 carr...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：

    authors： Park WS,Moon YW,Yang YM,Kim YS,Kim YD,Fuller BG,Vortmeyer AO,Fogt F,Lubensky IA,Zhuang Z

    更新日期：1998-09-01 00:00:00

  • Chromosome-11q13 gene amplifications in head and neck squamous-cell carcinomas - relation with lymph-node invasion.

    abstract：:Gene amplifications occurring in the q13 band of chromosome 11 are frequently observed in head and neck squamous cell carcinomas. In order to determine the relative frequency of amplification in 5 distinct 11q13 loci and their relation with clinical data, tumor DNAs from 31 patients - including 26 who had undergone ne...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.5.2.309

    authors： Parise O,Janot F,Guerry R,Fortin A,Luboinski B,Tursz T,Schwaab G,Busson P

    更新日期：1994-08-01 00:00:00

  • Treatment of hepatocellular carcinoma and the exacerbation of liver function.

    abstract：:We performed interventional treatments on 50 patients with hepatocellular carcinoma (HCC) and analyzed the relationship between these treatments and the exacerbation of liver function after treatment. The different treatments included transcatheter arterial embolization (TAE), percutaneous ethanol injection therapy (P...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.19.6.1279

    authors： Okano H,Shiraki K,Inoue H,Deguchi M,Sugimoto K,Sakai T,Ohmori S,Murata K,Nakano T,Yamakado K,Takeda K

    更新日期：2001-12-01 00:00:00

  • 5-Caffeoylquinic acid inhibits invasion of non-small cell lung cancer cells through the inactivation of p70S6K and Akt activity: Involvement of p53 in differential regulation of signaling pathways.

    abstract：:In the present study, we investigated the effects and molecular mechanism of 5-caffeoylquinic acid (5-CQA), a natural phenolic compound isolated from Ligularia fischeri, on cell invasion, proliferation and adhesion in p53 wild-type A549 and p53-deficient H1299 non-small cell lung cancer (NSCLC) cells. 5-CQA abrogated ...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.2016.3436

    authors： In JK,Kim JK,Oh JS,Seo DW

    更新日期：2016-05-01 00:00:00

  • Clinical significance of integrin alpha 6 mRNA expression in gastric carcinoma.

    abstract：:Integrin alpha 6 is believed to be involved in malignant biological development. High tumor/normal (T/N) ratio of integrin alpha 6 was observed in intestinal type tumors in gastric carcinoma. In an immunohistochemical study, an intense and continuous staining was seen at the basement layer in the intestinal type tumor...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.11.5.959

    authors： Mimori K,Mori M,Begum N,Tanaka S,Haraguchi M,Ueo H,Karimine N,Akiyoshi T,Barnard G

    更新日期：1997-11-01 00:00:00

  • Trans-activating activity of the E6 proteins of the human papillomavirus (HPV) type-11 and -16 on the PE1E4 promoter of HPV-11 in C33A cells.

    abstract：:We investigated trans-activating effects of the full-length E6 protein of HPV-16 (16E6) and the E6 protein of HPV-11 (11E6) on the PE1E4 promoter of HPV-11 in C33A cells which lack normal function of p53. 16E6 showed no significant activation of the reporter plasmid containing PE1E4 and the upstream sequence, includin...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.13.6.1253

    authors： Tomita Y,Asano Y,Shirasawa H

    更新日期：1998-12-01 00:00:00

  • Fenofibrate inhibits tumour intravasation by several independent mechanisms in a 3-dimensional co-culture model.

    abstract：:Lymph node metastasis of breast cancer is a clinical marker of poor prognosis. Yet, there exist no therapies targeting mechanisms of intravasation into lymphatics. Herein we report on an effect of the antidyslipidemic drug fenofibrate with vasoprotective activity, which attenuates breast cancer intravasation in vitro,...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo.2017.3956

    authors： Nguyen CH,Huttary N,Atanasov AG,Chatuphonprasert W,Brenner S,Fristiohady A,Hong J,Stadler S,Holzner S,Milovanovic D,Dirsch VM,Kopp B,Saiko P,Krenn L,Jäger W,Krupitza G

    更新日期：2017-05-01 00:00:00

  • Transcriptome analysis of hepatitis B virus-associated small hepatocellular carcinoma by serial analysis of gene expression.

    abstract：:Serial analysis of gene expression (SAGE) is a sequence-based tool that enables quantitative study of comprehensive gene expression profiles. In this study, we generated a SAGE library of hepatitis B virus (HBV)-associated small hepatocellular carcinoma (HCC) and compared it with libraries of normal liver and other or...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：10.3892/ijo_00000321

    authors： Kim JW,Lee SH,Park YS,Jeong SH,Kim N,Lee DH,Lee HS

    更新日期：2009-07-01 00:00:00

  • Histone deacetylase inhibitor scriptaid induces cell cycle arrest and epigenetic change in colon cancer cells.

    abstract：:Histone deacetylase inhibitors (HDACIs) are involved in cell growth, apoptosis and differentiation. This study aimed to investigate the effects of HDACI scriptaid on histone modification, demethylation, cell growth, cell cycle and apoptosis in the RKO colorectal cancer cell line and screening for scriptaid-induced gen...

    journal_title：International journal of oncology

    pub_type： 杂志文章

    doi：

    authors： Lee EJ,Lee BB,Kim SJ,Park YD,Park J,Kim DH

    更新日期：2008-10-01 00:00:00