Abstract:
:In mouse and human, the beta-globin genes reside in a linear array that is associated with a positive regulatory element located 5' to the genes known as the locus control region (LCR). The sequences of the mouse and human beta-globin LCRs are homologous, indicating conservation of an essential function in beta-globin gene regulation. We have sequenced regions flanking the beta-globin locus in both mouse and human and found that homology associated with the LCR is more extensive than previously known, making up a conserved block of approximately 40 kb. In addition, we have identified DNaseI-hypersensitive sites within the newly sequenced regions in both mouse and human, and these structural features also are conserved. Finally, we have found that both mouse and human beta-globin loci are embedded within an array of odorant receptor genes that are expressed in olfactory epithelium, and we also identify an olfactory receptor gene located 3' of the beta-globin locus in chicken. The data demonstrate an evolutionarily conserved genomic organization for the beta-globin locus and suggest a possible role for the beta-globin LCR in control of expression of these odorant receptor genes and/or the presence of mechanisms to separate regulatory signals in different tissues.
journal_name
Proc Natl Acad Sci U S Aauthors
Bulger M,van Doorninck JH,Saitoh N,Telling A,Farrell C,Bender MA,Felsenfeld G,Axel R,Groudine Mdoi
10.1073/pnas.96.9.5129keywords:
subject
Has Abstractpub_date
1999-04-27 00:00:00pages
5129-34issue
9eissn
0027-8424issn
1091-6490journal_volume
96pub_type
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