Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway.


BACKGROUND:Toll-like receptor 3 (TLR3) is a critical component of the innate immune response to dsRNA viruses, which was considered to be mainly expressed in immune cells and some endothelial cells. In this study, we investigated the expression and proapoptotic activity of TLR3 in human and murine tumor cell lines. METHODS:RT-PCR and FACS analysis were used to detect expression of TLR3 in various human and murine tumor cell lines. All tumor cell lines were cultured with poly I:C, CHX, or both for 12 h, 24 h, 72 h, and then the cell viability was analyzed with CellTiter 96(R) AQueous One Solution, the apoptosis was measured by FACS with Annexin V and PI staining. Production of Type I IFN in poly I:C/CHX mediated apoptosis were detected through western blotting. TLR3 antibodies and IFN-beta antibodies were used in Blockade and Neutralization Assay. RESULTS:We show that TLR3 are widely expressed on human and murine tumor cell lines, and activation of TLR3 signaling in cancerous cells by poly I:C made Hela cells (human cervical cancer) and MCA38 cells (murine colon cancer) become dose-dependently sensitive to protein synthesis inhibitor cycloheximide (CHX)-induced apoptosis. Blockade of TLR3 recognition with anti-TLR3 antibody greatly attenuated the proapoptotic effects of poly I:C on tumor cells cultured with CHX. IFN-beta production was induced after poly I:C/CHX treatment and neutralization of IFN-beta slightly reduced poly I:C/CHX -induced apoptosis. CONCLUSION:Our study demonstrated the proapoptotic activity of TLR3 expressed by various tumor cells, which may open a new range of clinical applications for TLR3 agonists as an adjuvant of certain cancer chemotherapy.


BMC Cancer


BMC cancer


Jiang Q,Wei H,Tian Z




Has Abstract


2008-01-17 00:00:00










  • Investigating the performance and cost-effectiveness of the simple ultrasound-based rules compared to the risk of malignancy index in the diagnosis of ovarian cancer (SUBSONiC-study): protocol of a prospective multicenter cohort study in the Netherlands.

    abstract:BACKGROUND:Estimating the risk of malignancy is essential in the management of adnexal masses. An accurate differential diagnosis between benign and malignant masses will reduce morbidity and costs due to unnecessary operations, and will improve referral to a gynecologic oncologist for specialized cancer care, which im...

    journal_title:BMC cancer

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Meys EM,Rutten IJ,Kruitwagen RF,Slangen BF,Bergmans MG,Mertens HJ,Nolting E,Boskamp D,Beets-Tan RG,van Gorp T

    更新日期:2015-06-26 00:00:00

  • Hyperbolastic modeling of tumor growth with a combined treatment of iodoacetate and dimethylsulphoxide.

    abstract:BACKGROUND:An understanding of growth dynamics of tumors is important in understanding progression of cancer and designing appropriate treatment strategies. We perform a comparative study of the hyperbolastic growth models with the Weibull and Gompertz models, which are prevalently used in the field of tumor growth. M...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Eby WM,Tabatabai MA,Bursac Z

    更新日期:2010-09-23 00:00:00

  • Molecular mechanism of anti-cancer activity of phycocyanin in triple-negative breast cancer cells.

    abstract:BACKGROUND:Triple-negative breast cancers represent an important clinical challenge, as these cancers do not respond to conventional endocrine therapies or other available targeted agents. Phycocyanin (PC), a natural, water soluble and non-toxic molecule is shown to have potent anti-cancer property. METHODS:In this st...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Ravi M,Tentu S,Baskar G,Rohan Prasad S,Raghavan S,Jayaprakash P,Jeyakanthan J,Rayala SK,Venkatraman G

    更新日期:2015-10-23 00:00:00

  • Attitudes towards the Faecal Occult Blood Test (FOBT) versus the Faecal Immunochemical Test (FIT) for colorectal cancer screening: perceived ease of completion and disgust.

    abstract:BACKGROUND:Colorectal cancer screening is key to early detection and thus to early treatment, but uptake is often sub-optimal, particularly amongst lower income groups. It is proposed that the imminent introduction of the single-sample Faecal Immunochemical Test (FIT) in Scotland may lead to increased uptake as compare...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Chambers JA,Callander AS,Grangeret R,O'Carroll RE

    更新日期:2016-02-13 00:00:00

  • Transcatheter arterial chemoembolization plus sorafenib versus transcatheter arterial chemoembolization alone to treat advanced hepatocellular carcinoma: a meta-analysis.

    abstract:BACKGROUND:Many studies have combined sorafenib with transcatheter arterial chemoembolization (TACE) to treat patients with advanced hepatocellular carcinoma (HCC), but the results are disputable. Thus, we conducted this meta-analysis to assess the efficacy and safety of the combination treatment in patients with advan...

    journal_title:BMC cancer

    pub_type: 杂志文章,meta分析


    authors: Cai R,Song R,Pang P,Yan Y,Liao Y,Zhou C,Wang S,Zhou X,Wang H,Zhang H,Sun H,Ma H

    更新日期:2017-11-06 00:00:00

  • Genetic associations between the miRNA polymorphisms miR-130b (rs373001), miR-200b (rs7549819), and miR-495 (rs2281611) and colorectal cancer susceptibility.

    abstract:BACKGROUND:Recent studies have extensively investigated the role of miRNAs in colorectal cancer (CRC), and several associations have been reported. In addition, single nucleotide polymorphisms (SNPs) in promoter regions of miRNAs have been shown to affect miRNA expression. Therefore, we aimed to analyze the effect of m...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Kim EG,Kim JO,Park HS,Ryu CS,Oh J,Jun HH,Kim JW,Kim NK

    更新日期:2019-05-22 00:00:00

  • Differential effects of arsenic trioxide on chemosensitization in human hepatic tumor and stellate cell lines.

    abstract:BACKGROUND:Crosstalk between malignant hepatocytes and the surrounding peritumoral stroma is a key modulator of hepatocarcinogenesis and therapeutic resistance. To examine the chemotherapy resistance of these two cellular compartments in vitro, we evaluated a well-established hepatic tumor cell line, HepG2, and an adul...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Rangwala F,Williams KP,Smith GR,Thomas Z,Allensworth JL,Lyerly HK,Diehl AM,Morse MA,Devi GR

    更新日期:2012-09-10 00:00:00

  • Matrix metalloproteinase 1 and circulating tumor cells in early breast cancer.

    abstract:BACKGROUND:Matrix metalloproteinases (MMPs) are involved in cancer invasion and metastasis. Circulating tumor cells (CTCs) play role in tumor dissemination and are an independent survival predictor in breast cancer (BC) patients. The aim of this study was to assess correlation between CTCs and tumor MMP1 in BC. METHOD...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Cierna Z,Mego M,Janega P,Karaba M,Minarik G,Benca J,Sedlácková T,Cingelova S,Gronesova P,Manasova D,Pindak D,Sufliarsky J,Danihel L,Reuben JM,Mardiak J

    更新日期:2014-06-28 00:00:00

  • HPV infection related immune infiltration gene associated therapeutic strategy and clinical outcome in HNSCC.

    abstract:BACKGROUND:Head and neck squamous cell carcinoma (HNSCC) is the sixth most common tumor in human. Research has shown that HPV status HNSCC is a unique prognosis factor, which may due to its immune infiltration landscape. But the underlying mechanism is unclear. METHODS:In this study, we used a combination of several b...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Zeng H,Song X,Ji J,Chen L,Liao Q,Ma X

    更新日期:2020-08-24 00:00:00

  • Correction to: A novel coordination complex of platinum (PT) induces cell death in colorectal cancer by altering redox balance and modulating MAPK pathway.

    abstract::An amendment to this paper has been published and can be accessed via the original article. ...

    journal_title:BMC cancer

    pub_type: 已发布勘误


    authors: Al-Khayal K,Vaali-Mohammed MA,Elwatidy M,Traiki TB,Al-Obeed O,Azam M,Khan Z,Abdulla M,Ahmad R

    更新日期:2020-09-01 00:00:00

  • P53, Somatostatin receptor 2a and Chromogranin A immunostaining as prognostic markers in high grade gastroenteropancreatic neuroendocrine neoplasms.

    abstract:BACKGROUND:High grade gastroenteropancreatic (GEP) neuroendocrine neoplasms (NEN) with a Ki67 proliferation index > 20%, include well-differentiated tumours grade 3 (NET G3) and poorly differentiated (PD) neuroendocrine carcinomas (NEC). Abnormal p53-expression is a feature of PD tumours, while expression of chromogran...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Nielsen K,Binderup T,Langer SW,Kjaer A,Knigge P,Grøndahl V,Melchior L,Federspiel B,Knigge U

    更新日期:2020-01-10 00:00:00

  • Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein.

    abstract:BACKGROUND:Humans are exposed to an array of both harmful and beneficial hormonally active compounds in the environment and through diet. Two such chemicals are Bisphenol A (BPA), a plasticizer, and genistein, a component of soy. Prepubertal exposure to BPA increased mammary carcinogenesis, while genistein suppressed c...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Wang J,Jenkins S,Lamartiniere CA

    更新日期:2014-05-29 00:00:00

  • Interleukin 12 shows a better curative effect on lung cancer than paclitaxel and cisplatin doublet chemotherapy.

    abstract:BACKGROUND:Interleukin 12 (IL-12) is a cytokine that has been reported to exhibit potent tumoricidal effects in animal tumor models. A combined approach using Paclitaxel and platinum-based doublet chemotherapy is the most commonly used backbone regimen for treating lung cancer. Despite numerous studies regarding the an...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Yue T,Zheng X,Dou Y,Zheng X,Sun R,Tian Z,Wei H

    更新日期:2016-08-22 00:00:00

  • Increased tartrate-resistant acid phosphatase (TRAP) expression in malignant breast, ovarian and melanoma tissue: an investigational study.

    abstract:BACKGROUND:Tartrate-resistant acid phosphatase (TRAP) is a metalloprotein enzyme that belongs to the acid phosphatases and is known to be expressed by osteoclasts. It has already been investigated as a marker of bone metastases in cancer patients. In this study, which examined the value of serum TRAP concentrations as ...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Honig A,Rieger L,Kapp M,Krockenberger M,Eck M,Dietl J,Kämmerer U

    更新日期:2006-07-25 00:00:00

  • Promotion of the occurrence of endometrioid carcinoma by S100 calcium binding protein P.

    abstract:BACKGROUND:Endometrial cancer, one of the most common malignant tumors, is a serious threat to women's health. Endometrial hyperplasia is a precursor of endometrial cancer. S100 calcium binding protein P (S100P) has been found to play important roles in many types of cancer. The present study aimed to investigate the e...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Zhang D,Chen X,Xia H,Wang L,Zhao H,Xu B,Zhang A,Zhang W

    更新日期:2020-09-03 00:00:00

  • Increased expression of colony stimulating factor-1 is a predictor of poor prognosis in patients with clear-cell renal cell carcinoma.

    abstract:BACKGROUND:This study aims to evaluate the impact of colony stimulating factor-1 (CSF-1) expression on recurrence and survival of patients with clear-cell renal cell carcinoma (ccRCC) following surgery. METHODS:We retrospectively enrolled 267 patients (195 in the training cohort and 72 in the validation cohort) with c...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Yang L,Wu Q,Xu L,Zhang W,Zhu Y,Liu H,Xu J,Gu J

    更新日期:2015-02-18 00:00:00

  • Lentivirus-mediated CDglyTK gene-modified free flaps by intra-artery perfusion show targeted therapeutic efficacy in rat model of breast cancer.

    abstract:BACKGROUND:Free flap-mediated gene therapy in the tumor bed following surgical resection is a promising approach in cancer targeted treatment of residual disease. We investigated the selective killing efficacy of a lentivirus-mediated cytosine deaminase-thymidine kinase (CDglyTK) gene in transplanted breast cancer deli...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Zhang J,Liu Y,Zang M,Zhu S,Chen B,Li S,Xue B,Yan L

    更新日期:2019-09-14 00:00:00

  • Polymorphisms in GSTT1, GSTM1, NAT1 and NAT2 genes and bladder cancer risk in men and women.

    abstract:BACKGROUND:Cigarette smoking is an established risk factor for bladder cancer. Epidemiological and biological data suggest that genetic polymorphisms in activating and detoxifying enzymes may play a role in determining an individual's susceptibility to bladder cancer in particular when in combination with specific envi...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: McGrath M,Michaud D,De Vivo I

    更新日期:2006-10-06 00:00:00

  • Evaluation of postoperative ascites after somatostatin infusion following hepatectomy for hepatocellular carcinoma by laparotomy: a multicenter randomized double-blind controlled trial (SOMAPROTECT).

    abstract:BACKGROUND:The majority of patients undergoing hepatectomy for hepatocellular carcinoma (HCC) suffer from underlying liver disease and are exposed to the risk of postoperative ascites, which is favored by an imbalance between portal venous inflow and a diminished hepatic volume. Finding a reversible, non-invasive metho...

    journal_title:BMC cancer

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Mohkam K,Rayar M,Adam JP,Muscari F,Rode A,Merle P,Pradat P,Bauler S,Delfour I,Chiche L,Ducerf C,Boudjema K,Lesurtel M,Laurent C,Mabrut JY

    更新日期:2018-08-23 00:00:00

  • Li-Fraumeni syndrome with simultaneous osteosarcoma and liver cancer: increased expression of a CD44 variant isoform after chemotherapy.

    abstract:BACKGROUND:Li-Fraumeni syndrome (LFS) is a hereditary cancer predisposition syndrome that is commonly associated with a germline mutation in the tumor suppressor gene p53. Loss of p53 results in increased expression of CD44, a cancer stem cell (CSC) marker, which is involved in the scavenging of reactive oxygen species...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Yoshida GJ,Fuchimoto Y,Osumi T,Shimada H,Hosaka S,Morioka H,Mukai M,Masugi Y,Sakamoto M,Kuroda T

    更新日期:2012-10-02 00:00:00

  • Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines.

    abstract:BACKGROUND:More than 50% of metastatic melanoma patients have a specific mutation in the serine/threonine kinase BRAF. This results in constitutive activation of the RAS-RAF-MEK-ERK-MAP kinase pathway, which causes uncontrolled cell growth. Vemurafenib (PLX4032) is an oral chemotherapeutic agent that targets the specif...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Xiao J,Egger ME,McMasters KM,Hao H

    更新日期:2018-06-22 00:00:00

  • Clinical relevance of ErbB-2/HER2 nuclear expression in breast cancer.

    abstract:BACKGROUND:The biological relevance of nuclear ErbB-2/HER2 (NuclErbB-2) presence in breast tumors remains unexplored. In this study we assessed the clinical significance of ErbB-2 nuclear localization in primary invasive breast cancer. The reporting recommendations for tumor marker prognostic studies (REMARK) guideline...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Schillaci R,Guzmán P,Cayrol F,Beguelin W,Díaz Flaqué MC,Proietti CJ,Pineda V,Palazzi J,Frahm I,Charreau EH,Maronna E,Roa JC,Elizalde PV

    更新日期:2012-02-22 00:00:00

  • The early response of renal cell carcinoma to tyrosine kinase inhibitors evaluated by FDG PET/CT was not influenced by metastatic organ.

    abstract:BACKGROUND:Tyrosine kinase inhibitors (TKIs) have become the mainstay of treatment for advanced renal cell carcinoma (RCC), but it has been unclear whether the antitumor effect of TKIs depends on the organ where the RCC metastasis is located. We previously reported that the FDG accumulation assessed by FDG PET/CT, was ...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Kakizoe M,Yao M,Tateishi U,Minamimoto R,Ueno D,Namura K,Makiyama K,Hayashi N,Sano F,Kishida T,Kobayashi K,Noguchi S,Ikeda I,Ohgo Y,Taguri M,Morita S,Inoue T,Kubota Y,Nakaigawa N

    更新日期:2014-06-02 00:00:00

  • The T790M resistance mutation in EGFR is only found in cfDNA from erlotinib-treated NSCLC patients that harbored an activating EGFR mutation before treatment.

    abstract:BACKGROUND:Lung cancer patients with an activating mutation in the EGFR (epidermal growth factor receptor) can develop resistance to erlotinib treatment, which is often mediated by the T790M resistance mutation in EGFR. The difficulties in obtaining biopsies at progression make it challenging to investigate the appeara...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Demuth C,Madsen AT,Weber B,Wu L,Meldgaard P,Sorensen BS

    更新日期:2018-02-15 00:00:00

  • Cytoplasmic Kaiso is associated with poor prognosis in non-small cell lung cancer.

    abstract:BACKGROUND:Kaiso has been identified as a new member of the POZ-zinc finger family of transcription factors that are implicated in development and cancer. Although controversy still exists, Kaiso is supposed to be involved in human cancer. However, there is limited information regarding the clinical significance of cyt...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Dai SD,Wang Y,Miao Y,Zhao Y,Zhang Y,Jiang GY,Zhang PX,Yang ZQ,Wang EH

    更新日期:2009-06-09 00:00:00

  • Using high throughput microtissue culture to study the difference in prostate cancer cell behavior and drug response in 2D and 3D co-cultures.

    abstract:BACKGROUND:There is increasing appreciation that non-cancer cells within the tumour microenvironment influence cancer progression and anti-cancer drug efficacy. For metastatic prostate cancer (PCa), the bone marrow microenvironment influences metastasis, drug response, and possibly drug resistance. METHODS:Using a nov...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Mosaad E,Chambers K,Futrega K,Clements J,Doran MR

    更新日期:2018-05-24 00:00:00

  • Feasibility of isometric spinal muscle training in patients with bone metastases under radiation therapy - first results of a randomized pilot trial.

    abstract:BACKGROUND:Spinal bone metastases are commonly diagnosed in cancer patients. The consequences are pain both at rest and under exercise, impairment of activities of daily life (ADL), reduced clinical performance, the risk of pathological fractures, and neurological deficits. The aim of this randomized, controlled pilot ...

    journal_title:BMC cancer

    pub_type: 杂志文章,随机对照试验


    authors: Rief H,Omlor G,Akbar M,Welzel T,Bruckner T,Rieken S,Haefner MF,Schlampp I,Gioules A,Habermehl D,von Nettelbladt F,Debus J

    更新日期:2014-02-05 00:00:00

  • A multicentre randomised controlled trial to evaluate the efficacy, morbidity and functional outcome of endoscopic transanal proctectomy versus laparoscopic proctectomy for low-lying rectal cancer (ETAP-GRECCAR 11 TRIAL): rationale and design.

    abstract:BACKGROUND:Total mesorectal excision is the standard surgical treatment for mid- and low-rectal cancer. Laparoscopy represents a clear leap forward in the management of rectal cancer patients, offering significant improvements in post-operative measures such as pain, first bowel movement, and hospital length of stay. H...

    journal_title:BMC cancer

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Lelong B,de Chaisemartin C,Meillat H,Cournier S,Boher JM,Genre D,Karoui M,Tuech JJ,Delpero JR,French Research Group of Rectal Cancer Surgery (GRECCAR).

    更新日期:2017-04-11 00:00:00

  • Skin cancers in people with albinism in Togo in 2019: results of two rounds of national mobile skin care clinics.

    abstract:BACKGROUND:In people with albinism (PWA), the deficiency of melanin increase the risk of skin cancers. The aim of this study was to determine the prevalence of skin cancers and characteristics of these detected skin cancers (histological types, localization) in PWA in 10 cities in Togo in 2019. METHODS:This is a cross...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Saka B,Akakpo SA,Teclessou JN,Gnossike P,Adam S,Mahamadou G,Kassang P,Elegbede Y,Mouhari-Toure A,Darre T,Kombate K,Pitché P

    更新日期:2021-01-05 00:00:00

  • A survey of the clinicopathological and molecular characteristics of patients with suspected Lynch syndrome in Latin America.

    abstract:BACKGROUND:Genetic counselling and testing for Lynch syndrome (LS) have recently been introduced in several Latin America countries. We aimed to characterize the clinical, molecular and mismatch repair (MMR) variants spectrum of patients with suspected LS in Latin America. METHODS:Eleven LS hereditary cancer registrie...

    journal_title:BMC cancer

    pub_type: 杂志文章


    authors: Rossi BM,Palmero EI,López-Kostner F,Sarroca C,Vaccaro CA,Spirandelli F,Ashton-Prolla P,Rodriguez Y,de Campos Reis Galvão H,Reis RM,Escremim de Paula A,Capochin Romagnolo LG,Alvarez K,Della Valle A,Neffa F,Kalfayan PG,Spir

    更新日期:2017-09-05 00:00:00