Abstract:
:Histones and their variants are subjected to several post-translational modifications (PTMs). Histones PTMs play an important role in the regulation of gene expression and are critical for the development and progression of many types of cancer, including breast cancer. In this study, we used two-dimensional TAU/SDS electrophoresis, coupled with mass spectrometry for a comprehensive profiling of histone PTMs in breast cancer cell lines.Proteomic approach allowed us to identify 85 histone PTMs, seventeen of which are not reported in the UniProt database. Western blot analysis was performed to confirm a peculiar pattern of PTMs in the sporadic and hereditary breast cancer cell lines compared to normal cells. Overlapping mass spectrometry data with western blotting results, we identified, for the first time to our knowledge, a tyrosine phosphorylation on histone H1, which is significantly higher in breast cancer cells. Additionally, by inhibiting specific signaling paths, such as PI3K, PPARγ and FAK pathways, we established a correlation between their regulation and the presence of new histone PTMs. Our results may provide new insight on the possible implication of these modifications in breast cancer and may offer new perspectives for future clinical applications.
journal_name
Aging (Albany NY)journal_title
Agingauthors
Perri AM,Agosti V,Olivo E,Concolino A,Angelis M,Tammè L,Fiumara CV,Cuda G,Scumaci Ddoi
10.18632/aging.102577subject
Has Abstractpub_date
2019-12-08 00:00:00pages
11722-11755issue
23issn
1945-4589pii
102577journal_volume
11pub_type
杂志文章相关文献
Aging-US文献大全abstract::Here we demonstrated that sulforaphane-cysteine (SFN-Cys) regulated cell cycle-related protein expressions in G0/G1 and G2/M phases of U87MG cells via High Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (HPLC-MS/MS) and proteomics analysis. Further, mRNA products of CDK4, CDK6 and α-tubulin were...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103537
更新日期:2020-08-29 00:00:00
abstract::The Mitochondrial Free Radical Theory of Aging (MFRTA) is currently one of the most widely accepted theories used to explain aging. From MFRTA three basic predictions can be made: long-lived individuals or species should produce fewer mitochondrial Reactive Oxygen Species (mtROS) than short-lived individuals or specie...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100137
更新日期:2010-04-01 00:00:00
abstract::The mechanism underlying the association between age and depletion of the human ovarian follicle reserves remains uncertain. Many identified that impaired DNA polymerase β (Pol β)-mediated DNA base-excision repair (BER) drives to mouse oocyte aging. With aging, DNA lesions accumulate in primordial follicles. However, ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104123
更新日期:2020-11-20 00:00:00
abstract::Senescent cells (SCs) have been considered a source of age-related chronic sterile systemic inflammation and a target for anti-aging therapies. To understand mechanisms controlling the amount of SCs, we analyzed the phenomenon of rapid clearance of human senescent fibroblasts implanted into SCID mice, which can be ove...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100991
更新日期:2016-07-01 00:00:00
abstract::The development and progression of hepatocellular carcinoma (HCC) is accompanied with persistent oxidative stress, but the molecular basis is not well defined. Superoxide dismutase 2 (SOD2) is an important mitochondrial antioxidant and a key aging factor. Here we investigated the expression and clinical significance o...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100967
更新日期:2016-06-01 00:00:00
abstract::In this study, we investigated the mechanistic role and prognostic significance of the long coding RNA (lncRNA) KCNQ1OT1 in colorectal cancer (CRC). KCNQ1OT1 levels were significantly higher in CRC tissues than adjacent normal colorectal tissues (n=79). High KCNQ1OT1 expression correlated with poorer prognosis in CRC ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103334
更新日期:2020-06-21 00:00:00
abstract::Atg7, a critical component of autophagy machinery, is essential for counteracting hematopoietic aging. However, the non-autophagic role of Atg7 on hematopoietic cells remains fundamentally unclear. In this study, we found that loss of Atg7, but not Atg5, another autophagy-essential gene, in the hematopoietic system re...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104176
更新日期:2020-11-24 00:00:00
abstract::Proliferating cell nuclear antigen binding factor (encoded by KIAA0101/PCLAF) regulates DNA synthesis and cell cycle progression; however, whether the level of KIAA0101 mRNA in lung adenocarcinoma is related to prognosis and tumor immune infiltration is unknown. In patients with lung adenocarcinoma, the differential e...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104144
更新日期:2020-11-20 00:00:00
abstract::A recently discovered satiety molecule, nesfatin-1, is localized in neurons of the hypothalamus and brain stem and colocalized with stress-related substances, corticotropin-releasing hormone (CRH), oxytocin, proopiomelanocortin, noradrenaline (NA) and 5-hydroxytryptamine (5-HT). Intracerebroventricular (icv) administr...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100207
更新日期:2010-11-01 00:00:00
abstract::The reproductive tissues are negatively influenced by estrogens in hormone therapy. Qingyan formula ethanol extract (QYFE)'s estrogenic effects and safety on reproductive tissues after long-term administration and its mechanism via estrogen receptor (ER) pathway haven't been studied. Here, we characterized its estroge...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102035
更新日期:2019-06-19 00:00:00
abstract::Exosomes play important roles in proliferation and microenvironment modulation of many types of cancers, including colorectal cancer (CRC). However, the inhibitory effect of CRC cells-derived exosomes in angiogenesis has not been fully discussed. In this study, the roles of microRNA-183-5p (miR-183-5p) in abundant in ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103145
更新日期:2020-05-03 00:00:00
abstract:BACKGROUND/AIMS:Kinesin family member 20A (KIF20A) is upregulated in multiple cancers and plays important roles in promoting malignant behavior, whereas its exact role in CRC remains unknown. RESULTS:Both genomic and protein expression levels showed that KIF20A was upregulated in CRC. Further functional analyses revea...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102505
更新日期:2019-12-16 00:00:00
abstract::Few reports have investigated different genetic alterations according to age in various cancers. In total, 1749 GC patients receiving curative surgery were enrolled. The clinicopathological features, and prognoses were compared between younger (<65 years) and older (≥65 years) patients. Genetic mutations were analyzed...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103627
更新日期:2020-08-18 00:00:00
abstract::Perioperative neurocognitive disorders are common in elderly patients who have undergone surgical procedures. Neuroinflammation induced by microglial activation is a hallmark of these neurological disorders. Acetate can suppress inflammation in the context of inflammatory diseases. We employed an exploratory laparotom...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102856
更新日期:2020-02-26 00:00:00
abstract::In this retrospective study we assessed the efficacy and safety of tocilizumab in patients with critical or severe coronavirus disease 2019 (COVID-19). We enrolled 181 patients admitted to Huoshenshan Hospital (Wuhan, China) with confirmed COVID-19 between January 2020 and February 2020. Ninety-two patients were treat...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103988
更新日期:2020-10-08 00:00:00
abstract::Age-related hearing loss (ARHL) has been considered as a promising modifiable risk factor for cognitive impairment and dementia. Nonetheless, it is still unclear whether age-related hearing loss associates with neurodegenerative biomarkers of Alzheimer's disease (AD). Participants with ARHL were selected from the esta...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101971
更新日期:2019-05-22 00:00:00
abstract::Obesity contributes to the incidence of various tumors, including colon cancer. However, the impact of obesity on patients' survival and related mechanisms remains unclear. Multi-omics data of 227 cases of colon cancer patients combined with clinical characteristics data were acquired from The Cancer Genome Atlas (TCG...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103972
更新日期:2020-11-10 00:00:00
abstract::Inflammation of the vascular microenvironment modulates distinct types of vascular cells, and plays important roles in promoting atherosclerosis, stenosis/restenosis, and vascular-related diseases. Nik-related kinase (Nrk), a member of the Ste20-type kinase family, has been reported to be selectively expressed in embr...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103104
更新日期:2020-04-24 00:00:00
abstract::Differences in the way human and mouse fibroblasts experience senescence in culture had long puzzled researchers. While senescence of human cells is mediated by telomere shortening, Parrinello et al. demonstrated that senescence of mouse cells is caused by extreme oxygen sensitivity. It was hypothesized that the strik...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100958
更新日期:2016-05-01 00:00:00
abstract::Evidence has shown that microRNAs (miRNAs) participate in the progression of CRC. Previous studies have indicated that miR-214-3p is abnormally expressed in various malignant tumors. However, the biological function it plays in CRC and the potential mechanism are unclear. Here, we demonstrated that miR-214-3p was obvi...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103233
更新日期:2020-05-15 00:00:00
abstract::In this study, we constructed a model using a Cox proportional hazards model based on the expression of eight immune-related genes that were associated with prognosis in prostate cancer: EDNRB, ANGPTL2, TNFSF15, TNFRSF10D, EDN2, BMP2, NLRP14, and PLK1. We then identified associations between risk scores calculated wit...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103921
更新日期:2020-11-10 00:00:00
abstract::The lack of a univocal definition of frailty, a condition frequently found in the elderly population which is correlated with an increased risk of mortality, has prompted the search for clinical and laboratory parameters associated with this condition. Whereas OPG is a protein involved in different pathophysiological ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102083
更新日期:2019-07-17 00:00:00
abstract::Aged liver is usually impaired in response to hepatic injury. Tissue-specific stem cells participate in the repair of tissue injury. However, how oval cells (OCs) respond to injury and how the process is regulated by tissue microenvironment in aged mice have not been fully understood. In this study, taking advantage o...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101665
更新日期:2018-12-04 00:00:00
abstract::Liver fibrosis biomarker, Type IV collagen, may function as hepatocarcinogenesis niche. However, among the six isoforms, the isoforms providing tumor microenvironment and their regulatory network are still unclarified. Based on bioinformatics analysis of hundreds of HCC transcriptome datasets from public databases, we...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102610
更新日期:2020-01-05 00:00:00
abstract::Upregulated ubiquitin-conjugating enzyme E2M (UBE2M) is associated with poor prognosis in malignancies; However, the phenotype and mechanism of action of UBE2M in hepatocellular carcinoma (HCC) remain elusive. Here, we report that UBE2M is overexpressed and correlated with poor prognosis in HCC patients. The UBE2M lev...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102749
更新日期:2020-02-03 00:00:00
abstract::Inflammatory bowel disease (IBD) is a group of chronic and recurrent nonspecific inflammatory disorders, including Crohn's disease (CD) and ulcerative colitis (UC). Due to the persistent inflammation of intestinal mucosa caused by immune disorders, barrier dysfunction may be an essential cause of the pathogenesis of I...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103566
更新日期:2020-08-28 00:00:00
abstract::Genetic factors combined with oxidative stress are major determinants of age-related hearing loss (ARHL), one of the most prevalent disorders of the elderly. Dwarf grey mice, Ggt1dwg/dwg, are homozygous for a loss of function mutation of the g-glutamyl transferase 1 gene, which encodes an important antioxidant enzyme ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100927
更新日期:2016-04-01 00:00:00
abstract::Chondroitin sulfate (CS) proteoglycans in perineuronal nets (PNNs) from the central nervous system (CNS) are involved in the control of plasticity and memory. Removing PNNs reactivates plasticity and restores memory in models of Alzheimer's disease and ageing. Their actions depend on the glycosaminoglycan (GAG) chains...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101256
更新日期:2017-06-28 00:00:00
abstract::Bone marrow transplantation is used to examine survival, hematopoietic stem cell function and pathology in recipients of young and old wild type bone marrow derived stem cells (BMDSCs) as well as cells from p53-based models of premature aging. There is no difference in the long term survival of recipients of 8 week-ol...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100867
更新日期:2015-12-01 00:00:00
abstract::Little is known on how well senescence markers in vitro and in situ correlate within individual donors. We studied correlations between the same and different in vitro markers. Furthermore, we tested correlations between in vitro markers with in situ p16INK4a positivity.From 100 donors (20-91 years), cultured dermal f...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101389
更新日期:2018-02-28 00:00:00