Abstract:
BACKGROUND:Human 15q11-13 is responsible for Prader-Willi syndrome (PWS) and Angelman syndrome (AS) and includes several imprinted genes together with bipartite elements named AS-IC (imprinting center) and PWS-IC. These concertedly confer allele specificity on 15q11-13. Here, we report DNA methylation status of 15q11-13 and other autosomal imprinted differentially methylated regions (iDMRs) in cases with various deletions within the PWS/AS-responsible region. METHODS:We performed array-based methylation analysis and examined the methylation status of CpG sites in 15q11-13 and in 71 iDMRs in six cases with various microdeletions, eight cases with conventional deletions within 15q11-13, and healthy controls. RESULTS:We detected 89 CpGs in 15q11-13 showing significant methylation changes in our cases. Of them, 14 CpGs in the SNORD116s cluster presented slight hypomethylation in the PWS cases and hypermethylation in the AS cases. No iDMRs at regions other than 15q11-13 showed abnormal methylation. CONCLUSIONS:We identified CpG sites and regions in which methylation status is regulated by AS-IC and PWS-IC. This result indicated that each IC had unique functions and coordinately regulated the DNA methylation of respective alleles. In addition, only aberrant methylation at iDMRs in 15q11-13 leads to the development of the phenotypes in our cases.
journal_name
Clin Epigeneticsjournal_title
Clinical epigeneticsauthors
Matsubara K,Itoh M,Shimizu K,Saito S,Enomoto K,Nakabayashi K,Hata K,Kurosawa K,Ogata T,Fukami M,Kagami Mdoi
10.1186/s13148-019-0633-1subject
Has Abstractpub_date
2019-02-28 00:00:00pages
36issue
1eissn
1868-7075issn
1868-7083pii
10.1186/s13148-019-0633-1journal_volume
11pub_type
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