Abstract:
BACKGROUND:In vitro follicle culture (IFC), as applied in the mouse system, allows the growth and maturation of a large number of immature preantral follicles to become mature and competent oocytes. In the human oncofertility clinic, there is increasing interest in developing this technique as an alternative to ovarian cortical tissue transplantation and to preserve the fertility of prepubertal cancer patients. However, the effect of IFC and hormonal stimulation on DNA methylation in the oocyte is not fully known, and there is legitimate concern over epigenetic abnormalities that could be induced by procedures applied during assisted reproductive technology (ART). RESULTS:In this study, we present the first genome-wide analysis of DNA methylation in MII oocytes obtained after natural ovulation, after IFC and after superovulation. We also performed a comparison between prepubertal and adult hormonally stimulated oocytes. Globally, the distinctive methylation landscape of oocytes, comprising alternating hyper- and hypomethylated domains, is preserved irrespective of the procedure. The conservation of methylation extends to the germline differential methylated regions (DMRs) of imprinted genes, necessary for their monoallelic expression in the embryo. However, we do detect specific, consistent, and coherent differences in DNA methylation in IFC oocytes, and between oocytes obtained after superovulation from prepubertal compared with sexually mature females. Several methylation differences span entire transcription units. Among these, we found alterations in Tcf4, Sox5, Zfp521, and other genes related to nervous system development. CONCLUSIONS:Our observations show that IFC is associated with altered methylation at specific set of loci. DNA methylation of superovulated prepubertal oocytes differs from that of superovulated adult oocytes, whereas oocytes from superovulated adult females differ very little from naturally ovulated oocytes. Importantly, we show that regions other than imprinted gDMRs are susceptible to methylation changes associated with superovulation, IFC, and/or sexual immaturity in mouse oocytes. Our results provide an important reference for the use of in vitro growth and maturation of oocytes, particularly from prepubertal females, in assisted reproductive treatments or fertility preservation.
journal_name
Clin Epigeneticsjournal_title
Clinical epigeneticsauthors
Saenz-de-Juano MD,Ivanova E,Billooye K,Herta AC,Smitz J,Kelsey G,Anckaert Edoi
10.1186/s13148-019-0794-ysubject
Has Abstractpub_date
2019-12-19 00:00:00pages
197issue
1eissn
1868-7075issn
1868-7083pii
10.1186/s13148-019-0794-yjournal_volume
11pub_type
杂志文章abstract:BACKGROUND:Epigenetic mechanisms have been suggested to play a role in the development of post-traumatic stress disorder (PTSD). Here, blood-derived DNA methylation data (HumanMethylation450 BeadChip) collected prior to and following combat exposure in three cohorts of male military members were analyzed to assess whet...
journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-019-0798-7
更新日期:2020-01-13 00:00:00
abstract:BACKGROUND:Prostate cancer (PCa), a highly incident and heterogeneous malignancy, mostly affects men from developed countries. Increased knowledge of the biological mechanisms underlying PCa onset and progression are critical for improved clinical management. MicroRNAs (miRNAs) deregulation is common in human cancers, ...
journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-015-0076-2
更新日期:2015-04-10 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/1868-7083-5-7
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journal_title:Clinical epigenetics
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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更新日期:2020-11-23 00:00:00
abstract::[This corrects the article DOI: 10.1186/s13148-016-0190-9.]. ...
journal_title:Clinical epigenetics
pub_type: 已发布勘误
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journal_title:Clinical epigenetics
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pub_type: 杂志文章
doi:10.1186/1868-7083-5-6
更新日期:2013-04-26 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章,评审
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更新日期:2021-01-19 00:00:00
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journal_title:Clinical epigenetics
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journal_title:Clinical epigenetics
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-016-0172-y
更新日期:2016-01-20 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-018-0539-3
更新日期:2018-08-06 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/1868-7083-3-9
更新日期:2011-12-05 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-019-0658-5
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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更新日期:2015-03-13 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-017-0335-5
更新日期:2017-04-14 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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