Genetical modification on adipose-derived stem cells facilitates facial nerve regeneration.


:Adipose-derived stem cells (ASCs) have a demonstrative therapeutic potential in aging-associated facial nerve regeneration, in which ASCs work as a source of Schwann cells therapy as an alternative to autologous nerve grafts. However, the transplantation of ASCs may induce local fibrosis, which causes inferior outcome. Here, we aimed to use genetic modification approaches to reduce the fibrogenic properties of ASCs to improve their therapeutic effects on facial nerve regeneration. Since procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1) is essential for hydroxylation of lysine residues in collagen telopeptides and for collagen pyridinoline cross-link formation during fibrosis, and since we found that ASCs expressed high levels of PLOD1, we depleted PLOD1 in ASCs by expression of either a short-hair interfering RNA for PLOD1 (shPLOD1) or a microRNA-449 (miR-449), the latter of which targets PLOD1 mRNA to suppress protein translation. Transplantation of either ASCs-shPLOD1 or ASCs-miR-449 or ASCs-control to repair a 6mm-gap in rat facial nerve was compared. Either ASCs-shPLOD1 or ASCs-miR-449 exhibited a better facial nerve function. Mechanistically, ASCs-shPLOD1 or ASCs-miR-449 significantly and similarly reduced the fibrosis in the injured region, likely through suppression of reactive oxygen species (ROS) and activation of myofibroblasts.


Aging (Albany NY)




Tan J,Xu Y,Han F,Ye X




Has Abstract


2019-02-06 00:00:00














  • SNCA Rep1 microsatellite length influences non-motor symptoms in early Parkinson's disease.

    abstract::Long alpha-synuclein gene (SNCA) promoter (Rep1) allele-carriers are linked to higher risk for Parkinson's disease (PD) and faster motor progression. Non-motor symptoms including autonomic, neuropsychiatric, and sleep disorders are common in PD. However, the relationship between SNCA Rep1 microsatellite lengths and no...


    pub_type: 杂志文章


    authors: Yong AC,Tan YJ,Zhao Y,Lu Z,Ng EY,Ng SY,Chia NS,Choi X,Heng D,Neo S,Xu Z,Tay KY,Au WL,Tan EK,Tan LC,Ng AS

    更新日期:2020-10-20 00:00:00

  • Mitochondria hyperfusion and elevated autophagic activity are key mechanisms for cellular bioenergetic preservation in centenarians.

    abstract::Mitochondria have been considered for long time as important determinants of cell aging because of their role in the production of reactive oxygen species. In this study we investigated the impact of mitochondrial metabolism and biology as determinants of successful aging in primary cultures of fibroblasts isolated fr...


    pub_type: 杂志文章


    authors: Sgarbi G,Matarrese P,Pinti M,Lanzarini C,Ascione B,Gibellini L,Dika E,Patrizi A,Tommasino C,Capri M,Cossarizza A,Baracca A,Lenaz G,Solaini G,Franceschi C,Malorni W,Salvioli S

    更新日期:2014-04-01 00:00:00

  • Increased serum salusin-α by aerobic exercise training correlates with improvements in arterial stiffness in middle-aged and older adults.

    abstract::Aging causes arterial stiffening which can be mitigated by increased physical activity. Although low circulating levels of salusin-α are associated with cardiovascular disease, whether salusin-α decreases with aging and whether the reduced arterial stiffening occurring with exercise training is associated with increas...


    pub_type: 杂志文章


    authors: Fujie S,Hasegawa N,Sanada K,Hamaoka T,Maeda S,Padilla J,Martinez-Lemus LA,Iemitsu M

    更新日期:2020-01-09 00:00:00

  • Age-related hearing loss accelerates cerebrospinal fluid tau levels and brain atrophy: a longitudinal study.

    abstract::Age-related hearing loss (ARHL) has been considered as a promising modifiable risk factor for cognitive impairment and dementia. Nonetheless, it is still unclear whether age-related hearing loss associates with neurodegenerative biomarkers of Alzheimer's disease (AD). Participants with ARHL were selected from the esta...


    pub_type: 杂志文章


    authors: Xu W,Zhang C,Li JQ,Tan CC,Cao XP,Tan L,Yu JT,Alzheimer’s Disease Neuroimaging Initiative.

    更新日期:2019-05-22 00:00:00

  • KCNQ1OT1 promotes melanoma growth and metastasis.

    abstract::Melanoma is the deadliest cutaneous neoplasm. To prevent metastasis, early diagnosis and surgical treatment is vital. Long non-coding RNAs (lncRNAs) may serve as biomarkers and therapeutic targets in tumors. We investigated the molecular mechanisms of lncRNA KCNQ1OT1 in melanoma. Real time PCR demonstrated that KCNQ1O...


    pub_type: 杂志文章


    authors: Guo B,Zhang Q,Wang H,Chang P,Tao K

    更新日期:2018-04-17 00:00:00

  • Neuroprotective effects and mechanisms of action of nicotinamide mononucleotide (NMN) in a photoreceptor degenerative model of retinal detachment.

    abstract::Currently, no pharmacotherapy has been proven effective in treating photoreceptor degeneration in patients. Discovering readily available and safe neuroprotectants is therefore highly sought after. Here, we investigated nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD+), in a re...


    pub_type: 杂志文章


    authors: Chen X,Amorim JA,Moustafa GA,Lee JJ,Yu Z,Ishihara K,Iesato Y,Barbisan P,Ueta T,Togka KA,Lu L,Sinclair DA,Vavvas DG

    更新日期:2020-12-29 00:00:00

  • Identification of survival-associated alternative splicing events and signatures in adrenocortical carcinoma based on TCGA SpliceSeq data.

    abstract:OBJECTIVE:To explore the correlations among alternative splicing (AS), splicing factors (SF) and survival outcome in adrenocortical carcinoma (ACC) patients. RESULTS:A total of 92 ACC patients were included. Univariate analysis identified 3919 AS events significantly associated with overall survival. Lasso method foll...


    pub_type: 杂志文章


    authors: Xu N,Ke ZB,Lin XD,Lin F,Chen SH,Wu YP,Chen YH,Wei Y,Zheng QS

    更新日期:2020-03-26 00:00:00

  • The clinicopathological characteristics and genetic alterations between younger and older gastric cancer patients with curative surgery.

    abstract::Few reports have investigated different genetic alterations according to age in various cancers. In total, 1749 GC patients receiving curative surgery were enrolled. The clinicopathological features, and prognoses were compared between younger (<65 years) and older (≥65 years) patients. Genetic mutations were analyzed...


    pub_type: 杂志文章


    authors: Wu CW,Chen MH,Huang KH,Chang SC,Fang WL,Lin CH,Chao Y,Lo SS,Li AF,Shyr YM

    更新日期:2020-08-18 00:00:00

  • Stress resistance and lifespan enhanced by downregulation of antimicrobial peptide genes in the Imd pathway.

    abstract::Biological behaviors and longevity of ectothermic animals are remarkably influenced by ambient temperature. Development at 18°C significantly enhances the stress resistance of adult flies with more accumulation of nutrients (especially fat) in the body than development at 25°C. Gene expression analysis between the fli...


    pub_type: 杂志文章


    authors: Lin YR,Parikh H,Park Y

    更新日期:2018-04-19 00:00:00

  • Prognostic values of GPNMB identified by mining TCGA database and STAD microenvironment.

    abstract::The survival rate of stomach adenocarcinoma patients with immune and stromal scores and different clinicopathological features obtained from the TCGA datasets was systematically compared. A list of genes that are correlated with stomach adenocarcinoma microenvironment were extracted using the TCGA database to predict ...


    pub_type: 杂志文章


    authors: Yao K,Wei L,Zhang J,Wang C,Wang C,Qin C,Li S

    更新日期:2020-08-21 00:00:00

  • Discovering master regulators in hepatocellular carcinoma: one novel MR, SEC14L2 inhibits cancer cells.

    abstract::Identification of master regulator (MR) genes offers a relatively rapid and efficient way to characterize disease-specific molecular programs. Since strong consensus regarding commonly altered MRs in hepatocellular carcinoma (HCC) is lacking, we generated a compendium of HCC datasets from 21 studies and identified a c...


    pub_type: 杂志文章


    authors: Li Z,Lou Y,Tian G,Wu J,Lu A,Chen J,Xu B,Shi J,Yang J

    更新日期:2019-12-18 00:00:00

  • Circ_0006332 promotes growth and progression of bladder cancer by modulating MYBL2 expression via miR-143.

    abstract::In this study, we analyzed the role of circular RNAs in the growth and progression of bladder cancer. Direct Sanger sequencing and quantitative RT-PCR analysis showed that circ_0006332 was significantly upregulated in bladder cancer tissues. Sequencing analysis showed that circ_0006332 is generated from splicing of ex...


    pub_type: 杂志文章


    authors: Li M,Liu Y,Liu J,Li W,Li N,Xue D,Zhang X,Wang P

    更新日期:2019-11-22 00:00:00

  • Periodontitis in elderly patients with type 2 diabetes mellitus: impact on gut microbiota and systemic inflammation.

    abstract::Elderly patients with type 2 diabetes mellitus (T2DM) exhibit considerable periodontitis frequency, which causes tooth loss and poor quality of life. To investigate the impact of periodontitis on gut microbiota, we used 16S rRNA amplicon sequencing to characterize the composition and structure of gut microbiota among ...


    pub_type: 杂志文章


    authors: Li J,Lu H,Wu H,Huang S,Chen L,Gui Q,Zhou W,Yang Y,Wu Y,Zhang H,Zhang Q,Yang Y

    更新日期:2020-11-25 00:00:00

  • Targeting CARD6 attenuates spinal cord injury (SCI) in mice through inhibiting apoptosis, inflammation and oxidative stress associated ROS production.

    abstract::Spinal cord injury (SCI) causes long-term and severe disability, influencing the quality of life and triggering serious socioeconomic consequences. Lack of effective pharmacotherapies for SCI is largely attributable to an incomplete understanding of its pathogenesis. Caspase recruitment domain family member 6 (CARD6) ...


    pub_type: 杂志文章


    authors: Wang JL,Luo X,Liu L

    更新日期:2019-12-16 00:00:00

  • Association between Nrf2 and CDKN2A expression in patients with end-stage renal disease: a pilot study.

    abstract::Patients with end-stage renal disease (ESRD) display phenotypic features of premature biological aging, characterized by disproportionately high morbidity and mortality at a younger age. Nuclear factor erythroid 2-related factor 2 (Nrf2) activity, a master regulator of antioxidative responses, declines with age and is...


    pub_type: 杂志文章


    authors: Sumida K,Han Z,Dashputre AA,Potukuchi PK,Kovesdy CP

    更新日期:2020-07-13 00:00:00

  • Dexrazoxane ameliorates radiation-induced heart disease in a rat model.

    abstract::Treatment of thoracic tumors with radiotherapy can lead to severe cardiac injury. We investigated the effects of dexrazoxane, a USFDA-approved cardioprotective drug administered with chemotherapy, on radiation-induced heart disease (RIHD) in a rat model. Male Sprague-Dawley rats were irradiated with a single dose of 2...


    pub_type: 杂志文章


    authors: Li L,Nie X,Zhang P,Huang Y,Ma L,Li F,Yi M,Qin W,Yuan X

    更新日期:2021-01-02 00:00:00

  • System level characterization of small molecule drugs and their affected long noncoding RNAs.

    abstract::Long noncoding RNAs (lncRNAs) have multiple regulatory roles and are involved in many human diseases. A potential therapeutic strategy based on targeting lncRNAs was recently developed. To gain insight into the global relationship between small molecule drugs and their affected lncRNAs, we constructed a small molecule...


    pub_type: 杂志文章


    authors: Yang H,Jiang Y,Zhang Y,Xu Y,Zhang C,Han J,Su F,Liu X,Mi K,Liu B,Shang D

    更新日期:2019-12-18 00:00:00

  • PD-1 gene rs10204525 and rs7421861 polymorphisms are associated with increased risk and clinical features of esophageal cancer in a Chinese Han population.

    abstract::Programmed death-1 (PD-1) polymorphisms have been associated with esophageal cancer risk. Here, the aims of this case-control study were to explore whether three PD-1 polymorphisms (rs10204525, rs7421861, and rs36084323) were related with the risk and clinical features of esophageal cancer in Chinese Han subjects (n =...


    pub_type: 杂志文章


    authors: Zang B,Chen C,Zhao JQ

    更新日期:2020-02-21 00:00:00

  • Reduced SOD2 expression is associated with mortality of hepatocellular carcinoma patients in a mutant p53-dependent manner.

    abstract::The development and progression of hepatocellular carcinoma (HCC) is accompanied with persistent oxidative stress, but the molecular basis is not well defined. Superoxide dismutase 2 (SOD2) is an important mitochondrial antioxidant and a key aging factor. Here we investigated the expression and clinical significance o...


    pub_type: 杂志文章


    authors: Wang R,Yin C,Li XX,Yang XZ,Yang Y,Zhang MY,Wang HY,Zheng XF

    更新日期:2016-06-01 00:00:00

  • Fish oil supplements, longevity and aging.

    abstract::Fish oil supplementation is of great medical and public interest with epidemiological evidence of health benefits in humans, in particular by conferring protection against heart diseases. Its anti-inflammatory properties have also been reported. Initial results from short-lived mouse strains showed that fish oil can i...


    pub_type: 评论,杂志文章


    authors: de Magalhães JP,Müller M,Rainger GE,Steegenga W

    更新日期:2016-08-01 00:00:00

  • CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age.

    abstract::Natural killer cells lacking expression of CD56 (CD56neg NK cells) have been described in chronic HIV and hepatitis C virus infection. Features and functions of CD56neg NK cells in the context of latent infection with CMV and / or EBV with age are not known. In a cohort of healthy donors >60 years of age, we found tha...


    pub_type: 杂志文章


    authors: Müller-Durovic B,Grählert J,Devine OP,Akbar AN,Hess C

    更新日期:2019-01-27 00:00:00

  • Prognostic roles of metabolic reprogramming-associated genes in patients with hepatocellular carcinoma.

    abstract::Metabolic reprogramming for adaptation to the tumor microenvironment is recognized as a hallmark of cancer. Although many altered metabolic genes have been reported to be associated with tumor pathological processes, systematic analysis of metabolic genes implicated in hepatocellular carcinoma prognosis remains rare. ...


    pub_type: 杂志文章


    authors: Cui L,Xue H,Wen Z,Lu Z,Liu Y,Zhang Y

    更新日期:2020-11-12 00:00:00

  • Birth mass is the key to understanding the negative correlation between lifespan and body size in dogs.

    abstract::Larger dog breeds live shorter than the smaller ones, opposite of the mass-lifespan relationship observed across mammalian species. Here we use data from 90 dog breeds and a theoretical model based on the first principles of energy conservation and life history tradeoffs to explain the negative correlation between lon...


    pub_type: 杂志文章


    authors: Fan R,Olbricht G,Baker X,Hou C

    更新日期:2016-12-08 00:00:00

  • LRP5 in age-related changes in vascular and alveolar morphogenesis in the lung.

    abstract::Aging is associated with impaired angiogenesis and lung alveolar regeneration, which contributes to the increased susceptibility to chronic lung diseases (CLD). We have reported that the Wnt ligand co-receptor, low-density lipoprotein receptor-related protein 5 (LRP5), stimulates angiogenesis and lung alveolar regener...


    pub_type: 杂志文章


    authors: Mammoto A,Muyleart M,Mammoto T

    更新日期:2019-01-05 00:00:00

  • Brain ageing changes proteoglycan sulfation, rendering perineuronal nets more inhibitory.

    abstract::Chondroitin sulfate (CS) proteoglycans in perineuronal nets (PNNs) from the central nervous system (CNS) are involved in the control of plasticity and memory. Removing PNNs reactivates plasticity and restores memory in models of Alzheimer's disease and ageing. Their actions depend on the glycosaminoglycan (GAG) chains...


    pub_type: 杂志文章


    authors: Foscarin S,Raha-Chowdhury R,Fawcett JW,Kwok JCF

    更新日期:2017-06-28 00:00:00

  • LINC01419 facilitates hepatocellular carcinoma growth and metastasis through targeting EZH2-regulated RECK.

    abstract::Long non-coding RNAs (lncRNAs) have been reported to play significant roles in human tumorigenesis, for example, in hepatocellular carcinoma (HCC). This study explored the role of LINC01419, a new lncRNA, in HCC. In vitro study revealed that LINC01419 promotes growth and migration of HCC cells. Genes that affected cel...


    pub_type: 杂志文章


    authors: Zhang G,Chen X,Ma L,Ding R,Zhao L,Ma F,Deng X

    更新日期:2020-06-10 00:00:00

  • Autophagy modulates mesenchymal-to-endothelial transition via p53.

    abstract::Mesenchymal-to-endothelial transition (MEndT) is one of the mechanisms that influences cardiac fibrosis, which is a key process in cardiac remodeling. It has been reported that autophagy inhibits endothelial cell transition. However, whether autophagy could modulate MEndT in cardiac fibrosis has not yet been investiga...


    pub_type: 杂志文章


    authors: Hu J,Kong S,Dong T,Lin Z,Zhang Q,Chen X,Gong Y,Fan X,He M,Zhou H

    更新日期:2020-11-13 00:00:00

  • Personality, self-rated health, and cognition in centenarians: do personality and self-rated health relate to cognitive function in advanced age?

    abstract::Personality and self-rated health have been linked previously to cognitive outcome in late life. However, these associations have not been shown among the oldest old. This study examined relationships between personality, self-rated health, and cognitive function in a selected sample of Ashkenazi Jewish centenarians (...


    pub_type: 杂志文章


    authors: Kato K,Zweig R,Schechter CB,Verghese J,Barzilai N,Atzmon G

    更新日期:2013-03-01 00:00:00

  • Overexpression of CBS and CSE genes affects lifespan, stress resistance and locomotor activity in Drosophila melanogaster.

    abstract::Recent experimental studies highlighted the role of hydrogen sulfide (H2S) in aging and longevity. The cystathionine ß-synthase (CBS) and cystathionine γ-lyase (CSE) are the key enzymes responsible for H2S production. Here we investigated the geroprotective effects of CSE and CBS overexpression in Drosophila. Overexpr...


    pub_type: 杂志文章


    authors: Shaposhnikov M,Proshkina E,Koval L,Zemskaya N,Zhavoronkov A,Moskalev A

    更新日期:2018-11-08 00:00:00

  • Aging: Dial M for Mitochondria.

    abstract::A major goal of aging research is to identify interventions that prolong lifespan in distantly related organisms. In recent years, genetic studies in both nematodes and rodents have reported that moderate inactivation of genes important for mitochondrial electron transport chain (ETC) function can promote longevity. W...


    pub_type: 杂志文章,评审


    authors: Hur JH,Cho J,Walker DW

    更新日期:2010-01-26 00:00:00