Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva.


BACKGROUND:Hosts repeatedly bitten by sand flies develop antibodies against sand fly saliva and screening of these immunoglobulins can be employed to estimate the risk of Leishmania transmission, to indicate the feeding preferences of sand flies, or to evaluate the effectiveness of vector control campaigns. Previously, antibodies to sand fly saliva were detected using whole salivary gland homogenate (SGH) or recombinant proteins, both of which also have their disadvantages. This is the first study on sand flies where short peptides designed based on salivary antigens were successfully utilized for antibody screening. METHODOLOGY/PRINCIPAL FINDINGS:Specific IgG was studied in hosts naturally exposed to Phlebotomus orientalis, the main vector of Leishmania donovani in East Africa. Four peptides were designed by the commercial program EpiQuest-B, based on the sequences of the two most promising salivary antigens, yellow-related protein and ParSP25-like protein. Short amino acid peptides were synthesised and modified for ELISA experiments. Specific anti-P. orientalis IgG was detected in sera of dogs, goats, and sheep from Ethiopia. The peptide OR24 P2 was shown to be suitable for antibody screening; it correlated positively with SGH and its specificity and sensitivity were comparable or even better than that of previously published recombinant proteins. CONCLUSIONS/SIGNIFICANCE:OR24 P2, the peptide based on salivary antigen of P. orientalis, was shown to be a valuable tool for antibody screening of domestic animals naturally exposed to P. orientalis. We suggest the application of this promising methodology using species-specific short peptides to other sand fly-host combinations.


PLoS Negl Trop Dis


Sima M,Ferencova B,Bhattacharyya T,Miles MA,Litvinov SV,Hailu A,Baneth G,Volf P




Has Abstract


2019-01-24 00:00:00














  • Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products.

    abstract:INTRODUCTION:While we lack a complete understanding of the molecular mechanisms by which parasites establish and achieve protection from host immune responses, it is accepted that many of these processes are mediated by products, primarily proteins, released from the parasite. Parasitic nematodes occur in different lif...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Moreno Y,Geary TG

    更新日期:2008-01-01 00:00:00

  • Unforeseen costs of cutting mosquito surveillance budgets.

    abstract::A budget proposal to stop the U.S. Centers for Disease Control and Prevention (CDC) funding in surveillance and research for mosquito-borne diseases such as dengue and West Nile virus has the potential to leave the country ill-prepared to handle new emerging diseases and manage existing ones. In order to demonstrate t...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Vazquez-Prokopec GM,Chaves LF,Ritchie SA,Davis J,Kitron U

    更新日期:2010-10-26 00:00:00

  • Chlamydia trachomatis ompA variants in trachoma: what do they tell us?

    abstract:BACKGROUND:Trachoma, caused by Chlamydia trachomatis (Ct), is the leading infectious cause of blindness. Sequence-based analysis of the multiple strains typically present in endemic communities may be informative for epidemiology, transmission, response to treatment, and understanding the host response. METHODS:Conjun...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Andreasen AA,Burton MJ,Holland MJ,Polley S,Faal N,Mabey DC,Bailey RL

    更新日期:2008-09-24 00:00:00

  • Biodegradable nanocarriers coated with polymyxin B: Evaluation of leishmanicidal and antibacterial potential.

    abstract::Most treatments of leishmaniasis require hospitalization and present side effects or parasite resistance; innovations in drug formulation/reposition can overcome these barriers and must be pursued to increase therapeutic alternatives. Therefore, we tested polymyxin B (polB) potential to kill Leishmania amazonensis, ad...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Souza Ribeiro Costa J,Medeiros M,Yamashiro-Kanashiro EH,Rocha MC,Cotrim PC,Stephano MA,Lancellotti M,Tavares GD,Oliveira-Nascimento L

    更新日期:2019-05-01 00:00:00

  • Barriers to Buruli ulcer treatment completion in the Ashanti and Central Regions, Ghana.

    abstract:BACKGROUND:Buruli ulcer is a chronic ulcerating skin condition, with the highest burden found in Central and West Africa where it disproportionately affects the most vulnerable populations. Treatment is demanding, comprising eight-weeks of daily antibiotics, regular wound care and possible surgical intervention. Treatm...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Collinson S,Frimpong VNB,Agbavor B,Montgomery B,Oppong M,Frimpong M,Amoako YA,Marks M,Phillips RO

    更新日期:2020-05-26 00:00:00

  • Last generation triazoles for imported eumycetoma in eleven consecutive adults.

    abstract:BACKGROUND:Optimal management of eumycetoma, a severely debilitating chronic progressive fungal infection of skin, disseminating to bone and viscera, remains challenging. Especially, optimal antifungal treatment and duration are ill defined. METHODOLOGY/PRINCIPAL FINDINGS:We conducted a monocentric retrospective study...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Crabol Y,Poiree S,Bougnoux ME,Maunoury C,Barete S,Zeller V,Arvieux C,Pineau S,Amazzough K,Lecuit M,Lanternier F,Lortholary O,French Mycosis Study Group.

    更新日期:2014-10-09 00:00:00

  • Comparison between itraconazole and cotrimoxazole in the treatment of paracoccidiodomycosis.

    abstract:BACKGROUND:There are no published reports on studies comparing itraconazole (ITC), sulfamethoxazole-trimethoprim (cotrimoxazole, CMX), and ITC followed by CMX (ITC/CMX) in the treatment of paracoccidiodomycosis. This study aimed to compare the efficacy, effectiveness, safety and time to clinical and serologic cure in p...

    journal_title:PLoS neglected tropical diseases

    pub_type: 临床试验,杂志文章


    authors: Cavalcante Rde S,Sylvestre TF,Levorato AD,de Carvalho LR,Mendes RP

    更新日期:2014-04-17 00:00:00

  • Bacterial community composition in the salivary glands of triatomines (Hemiptera: Reduviidae).

    abstract:BACKGROUND:Chagas disease is caused by the parasite Trypanosoma cruzi and is transmitted through triatomines (Hemiptera: Reduviidae). In the last year, many studies of triatomine gut microbiota have outlined its potential role in modulating vector competence. However, little is known about the microbiota present in the...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Lima MS,Laport MS,Lorosa ES,Jurberg J,Dos Santos KRN,da Silva Neto MAC,Rachid CTCDC,Atella GC

    更新日期:2018-09-13 00:00:00

  • Complexities and Perplexities: A Critical Appraisal of the Evidence for Soil-Transmitted Helminth Infection-Related Morbidity.

    abstract:BACKGROUND:Soil-transmitted helminths (STH) have acute and chronic manifestations, and can result in lifetime morbidity. Disease burden is difficult to quantify, yet quantitative evidence is required to justify large-scale deworming programmes. A recent Cochrane systematic review, which influences Global Burden of Dise...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,meta分析,评审


    authors: Campbell SJ,Nery SV,Doi SA,Gray DJ,Soares Magalhães RJ,McCarthy JS,Traub RJ,Andrews RM,Clements AC

    更新日期:2016-05-19 00:00:00

  • Oxidative stress and DNA lesions: the role of 8-oxoguanine lesions in Trypanosoma cruzi cell viability.

    abstract::The main consequence of oxidative stress is the formation of DNA lesions, which can result in genomic instability and lead to cell death. Guanine is the base that is most susceptible to oxidation, due to its low redox potential, and 8-oxoguanine (8-oxoG) is the most common lesion. These characteristics make 8-oxoG a g...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Aguiar PH,Furtado C,Repolês BM,Ribeiro GA,Mendes IC,Peloso EF,Gadelha FR,Macedo AM,Franco GR,Pena SD,Teixeira SM,Vieira LQ,Guarneri AA,Andrade LO,Machado CR

    更新日期:2013-06-13 00:00:00

  • L-arginine availability and arginase activity: Characterization of amino acid permease 3 in Leishmania amazonensis.

    abstract:BACKGROUND:Leishmania uses the amino acid L-arginine as a substrate for arginase, enzyme that produces urea and ornithine, last precursor of polyamine pathway. This pathway is used by the parasite to replicate and it is essential to establish the infection in the mammalian host. L-arginine is not synthesized by the par...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Aoki JI,Muxel SM,Zampieri RA,Acuña SM,Fernandes JCR,Vanderlinde RH,Sales MCOP,Floeter-Winter LM

    更新日期:2017-10-26 00:00:00

  • Correction: Prevalence of Lymphatic Filariasis and Treatment Effectiveness of Albendazole/ Ivermectin in Individuals with HIV Co-infection in Southwest-Tanzania.

    abstract::[This corrects the article DOI: 10.1371/journal.pntd.0004618.]. ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 已发布勘误


    authors: Kroidl I,Saathoff E,Maganga L,Clowes P,Maboko L,Hoerauf A,Makunde WH,Haule A,Mviombo P,Pitter B,Mgeni N,Mabuye J,Kowuor D,Mwingira U,Malecela MN,Löscher T,Hoelscher M

    更新日期:2016-08-26 00:00:00

  • Molecular typing of Treponema pallidum: a systematic review and meta-analysis.

    abstract:BACKGROUND:Syphilis is resurgent in many regions of the world. Molecular typing is a robust tool for investigating strain diversity and epidemiology. This study aimed to review original research on molecular typing of Treponema pallidum (T. pallidum) with three objectives: (1) to determine specimen types most suitable ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,meta分析,评审


    authors: Peng RR,Wang AL,Li J,Tucker JD,Yin YP,Chen XS

    更新日期:2011-11-01 00:00:00

  • Multi locus sequence typing of Burkholderia pseudomallei isolates from India unveils molecular diversity and confers regional association in Southeast Asia.

    abstract:OBJECTIVES:Burkholderia pseudomallei, the causative agent for melioidosis, has become a public health problem in India and across the world. Melioidosis can be difficult to diagnose because of the inconsistent clinical presentations of the disease. This study aims to determine the genetic diversity among the clinical i...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Balaji V,Perumalla S,Perumal R,Inbanathan FY,Rajamani Sekar SK,Paul MM,Sahni RD,Prakash JAJ,Iyadurai R

    更新日期:2018-06-27 00:00:00

  • Therapeutic control of leishmaniasis by inhibitors of the mammalian target of rapamycin.

    abstract::Leishmaniasis is a serious global health problem affecting many people worldwide. While patients with leishmaniasis can be treated with several agents, drug toxicicty and the emergence of resistant strains render available treatments ineffective in the long run. Inhibitors of the mammalian target of rapamycin (mTOR) h...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Khadir F,Shaler CR,Oryan A,Rudak PT,Mazzuca DM,Taheri T,Dikeakos JD,Haeryfar SMM,Rafati S

    更新日期:2018-08-22 00:00:00

  • Bioecological Drivers of Rabies Virus Circulation in a Neotropical Bat Community.

    abstract:INTRODUCTION:In addition to the commonly accepted importance of the vampire bat in the maintenance and transmission of the rabies virus (RABV) in South America, RABV infection of other species is widely evidenced, challenging their role in the viral cycle. METHODOLOGY / PRINCIPLES FINDINGS:To identify the bioecologica...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: de Thoisy B,Bourhy H,Delaval M,Pontier D,Dacheux L,Darcissac E,Donato D,Guidez A,Larrous F,Lavenir R,Salmier A,Lacoste V,Lavergne A

    更新日期:2016-01-25 00:00:00

  • Annexin A2 depletion exacerbates the intracerebral microhemorrhage induced by acute rickettsia and Ebola virus infections.

    abstract::Intracerebral microhemorrhages (CMHs) are small foci of hemorrhages in the cerebrum. Acute infections induced by some intracellular pathogens, including rickettsia, can result in CMHs. Annexin a2 (ANXA2) has been documented to play a functional role during intracellular bacterial adhesion. Here we report that ANXA2-kn...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Su Z,Chang Q,Drelich A,Shelite T,Judy B,Liu Y,Xiao J,Zhou C,He X,Jin Y,Saito T,Tang S,Soong L,Wakamiya M,Fang X,Bukreyev A,Ksiazek T,Russell WK,Gong B

    更新日期:2020-07-20 00:00:00

  • Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota.

    abstract:BACKGROUND:Mycobacterium tuberculosis complex (MTBC), the causative agent of tuberculosis (TB), is composed of eight subspecies. TB in West Africa, in contrast to other geographical regions, is caused by Mycobacterium africanum (MAF) in addition to M. tuberculosis (MTB), with both infections presenting similar symptoms...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Namasivayam S,Diarra B,Diabate S,Sarro YDS,Kone A,Kone B,Tolofoudie M,Baya B,Diakite MT,Kodio O,Cohen K,Holl J,Achenbach CJ,Chatterjee S,Murphy RL,Bishai W,Diallo S,Sher A,Maiga M

    更新日期:2020-05-13 00:00:00

  • Stress susceptibility in Trypanosoma brucei lacking the RNA-binding protein ZC3H30.

    abstract::Trypanosomes rely on post-transcriptional mechanisms and mRNA-binding proteins for control of gene expression. Trypanosoma brucei ZC3H30 is an mRNA-binding protein that is expressed in both the bloodstream form (which grows in mammals) and the procyclic form (which grows in the tsetse fly midgut). Attachment of ZC3H30...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Chakraborty C,Clayton C

    更新日期:2018-10-01 00:00:00

  • Neglected tropical diseases in children: An assessment of gaps in research prioritization.

    abstract:BACKGROUND:Despite the known burden of neglected tropical diseases (NTDs) on child health, there is limited information on current efforts to increase pediatric therapeutic options. Our objective was to quantify and characterize research activity and treatment availability for NTDs in children in order to inform the pr...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Rees CA,Hotez PJ,Monuteaux MC,Niescierenko M,Bourgeois FT

    更新日期:2019-01-29 00:00:00

  • Head and neck mycetoma: the mycetoma research centre experience.

    abstract::Mycetoma is a unique neglected tropical disease which is endemic in what is known as the "mycetoma belt". The disease has many devastating impacts on patients and communities in endemic area and is characterised by massive deformity, destruction and disability. Mycetoma is commonly seen in the foot and hand and less f...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fahal A,Mahgoub el S,El Hassan AM,Jacoub AO,Hassan D

    更新日期:2015-03-13 00:00:00

  • Insights into the trypanosome-host interactions revealed through transcriptomic analysis of parasitized tsetse fly salivary glands.

    abstract::The agents of sleeping sickness disease, Trypanosoma brucei complex parasites, are transmitted to mammalian hosts through the bite of an infected tsetse. Information on tsetse-trypanosome interactions in the salivary gland (SG) tissue, and on mammalian infective metacyclic (MC) parasites present in the SG, is sparse. ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Telleria EL,Benoit JB,Zhao X,Savage AF,Regmi S,Alves e Silva TL,O'Neill M,Aksoy S

    更新日期:2014-04-24 00:00:00

  • Geographical distribution of soil transmitted helminths and the effects of community type in South Asia and South East Asia - A systematic review.

    abstract:BACKGROUND:Soil-transmitted helminth (STH) infections are among the most prevalent neglected tropical diseases (NTD) worldwide. Since the publication of the WHO road map to combat NTD in 2012, there has been a renewed commitment to control STH. In this study, we analysed the geographical distribution and effect of comm...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Silver ZA,Kaliappan SP,Samuel P,Venugopal S,Kang G,Sarkar R,Ajjampur SSR

    更新日期:2018-01-18 00:00:00

  • Evaluation of the loop mediated isothermal DNA amplification (LAMP) kit for malaria diagnosis in P. vivax endemic settings of Colombia.

    abstract:BACKGROUND:Most commonly used malaria diagnostic tests, including microscopy and antigen-detecting rapid tests, cannot reliably detect low-density infections which are frequent in low transmission settings. Molecular methods such as polymerase chain reaction (PCR) are highly sensitive but remain too laborious for field...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Vallejo AF,Martínez NL,González IJ,Arévalo-Herrera M,Herrera S

    更新日期:2015-01-08 00:00:00

  • Engineered action at a distance: Blood-meal-inducible paralysis in Aedes aegypti.

    abstract:BACKGROUND:Population suppression through mass-release of Aedes aegypti males carrying dominant-lethal transgenes has been demonstrated in the field. Where population dynamics show negative density-dependence, suppression can be enhanced if lethality occurs after the density-dependent (i.e. larval) stage. Existing mole...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Haghighat-Khah RE,Harvey-Samuel T,Basu S,StJohn O,Scaife S,Verkuijl S,Lovett E,Alphey L

    更新日期:2019-09-03 00:00:00

  • Total Phenolic Fraction (TPF) from Extra Virgin Olive Oil: Induction of apoptotic-like cell death in Leishmania spp. promastigotes and in vivo potential of therapeutic immunomodulation.

    abstract:BACKGROUND:Leishmaniasis is a serious multifactorial parasitic disease with limited treatment options. Current chemotherapy is mainly consisted of drugs with serious drawbacks such as toxicity, variable efficacy and resistance. Alternative bioactive phytocompounds may provide a promising source for discovering new anti...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Karampetsou K,Koutsoni OS,Gogou G,Angelis A,Skaltsounis LA,Dotsika E

    更新日期:2021-01-11 00:00:00

  • Favipiravir (T-705) inhibits Junín virus infection and reduces mortality in a guinea pig model of Argentine hemorrhagic fever.

    abstract:BACKGROUND:Junín virus (JUNV), the etiologic agent of Argentine hemorrhagic fever (AHF), is classified by the NIAID and CDC as a Category A priority pathogen. Presently, antiviral therapy for AHF is limited to immune plasma, which is readily available only in the endemic regions of Argentina. T-705 (favipiravir) is a b...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Gowen BB,Juelich TL,Sefing EJ,Brasel T,Smith JK,Zhang L,Tigabu B,Hill TE,Yun T,Pietzsch C,Furuta Y,Freiberg AN

    更新日期:2013-12-26 00:00:00

  • Yaws resurgence in Bankim, Cameroon: The relative effectiveness of different means of detection in rural communities.

    abstract:BACKGROUND:Yaws is an infectious, debilitating and disfiguring disease of poverty that mainly affects children in rural communities in tropical areas. In Cameroon, mass-treatment campaigns carried out in the 1950s reduced yaws to such low levels that it was presumed the disease was eradicated. In 2010, an epidemiologic...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Boock AU,Awah PK,Mou F,Nichter M

    更新日期:2017-05-08 00:00:00

  • Rapid identification of paragonimiasis foci by lay informants in Lao People's Democratic Republic.

    abstract:BACKGROUND:Paragonimiasis is a food-borne trematodiasis leading to lung disease. Worldwide, an estimated 21 million people are infected. Foci of ongoing transmission remain often unnoticed. We evaluated a simple questionnaire approach using lay-informants at the village level to identify paragonimiasis foci and suspect...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Odermatt P,Veasna D,Zhang W,Vannavong N,Phrommala S,Habe S,Barennes H,Strobel M

    更新日期:2009-09-22 00:00:00

  • Nodular worm infection in wild chimpanzees in Western Uganda: a risk for human health?

    abstract::This study focused on Oeosophagostomum sp., and more especially on O. bifurcum, as a parasite that can be lethal to humans and is widespread among humans and monkeys in endemic regions, but has not yet been documented in apes. Its epidemiology and the role played by non-human primates in its transmission are still poo...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Krief S,Vermeulen B,Lafosse S,Kasenene JM,Nieguitsila A,Berthelemy M,L'hostis M,Bain O,Guillot J

    更新日期:2010-03-16 00:00:00