Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro.


:Di (2-ethylhexyl) phthalate (DEHP), an estrogen-like compound that is a ubiquitous environmental contaminant, has been reported to adversely affect human and mammalian reproduction. Many studies have found that exposure to DEHP during pregnancy perturbs female germ cell meiosis and is detrimental to oogenesis. Previous studies have demonstrated that melatonin (MLT) is beneficial to reproductive endocrinology, oogenesis, and embryonic development as the ability to antioxidative and antiapoptotic. However, whether the meiotic defect of germ cells exposed to DEHP could be rescued by MLT is not clear. Here, we cultured 12.5 days post coitum (dpc) fetal mouse ovaries for 6 days, exposed them to 100 μM DEHP with or without 1 μM MLT in vitro.. The results showed that DEHP exposure induced the abnormal formation of DNA double-strand breaks (DSBs), and inhibited the repair of DSBs during meiotic recombination. In addition, we found defective oocytes were prone to undergo apoptosis. Notably, this defect could be remarkably ameliorated by the addition of MLT via a reduction of the levels of reactive oxygen species and an inhibition of apoptosis. In conclusion, our data revealed that MLT had a protective action against the meiotic deterioration of fetal oocytes induced by DEHP in the mouse in vitro.


Aging (Albany NY)




Sun ZY,Zhang P,Wang JJ,Liu JC,Li L,Shen W,Zhai QY




Has Abstract


2018-12-26 00:00:00














  • KIF20A promotes cellular malignant behavior and enhances resistance to chemotherapy in colorectal cancer through regulation of the JAK/STAT3 signaling pathway.

    abstract:BACKGROUND/AIMS:Kinesin family member 20A (KIF20A) is upregulated in multiple cancers and plays important roles in promoting malignant behavior, whereas its exact role in CRC remains unknown. RESULTS:Both genomic and protein expression levels showed that KIF20A was upregulated in CRC. Further functional analyses revea...


    pub_type: 杂志文章


    authors: Xiong M,Zhuang K,Luo Y,Lai Q,Luo X,Fang Y,Zhang Y,Li A,Liu S

    更新日期:2019-12-16 00:00:00

  • Resveratrol targets PD-L1 glycosylation and dimerization to enhance antitumor T-cell immunity.

    abstract::New strategies to block the immune evasion activity of programmed death ligand-1 (PD-L1) are urgently needed. When exploring the PD-L1-targeted effects of mechanistically diverse metabolism-targeting drugs, exposure to the dietary polyphenol resveratrol (RSV) revealed its differential capacity to generate a distinct P...


    pub_type: 杂志文章


    authors: Verdura S,Cuyàs E,Cortada E,Brunet J,Lopez-Bonet E,Martin-Castillo B,Bosch-Barrera J,Encinar JA,Menendez JA

    更新日期:2020-01-04 00:00:00

  • Spermine and spermidine modulate T-cell function in older adults with and without cognitive decline ex vivo.

    abstract::The global increase in neurodegenerative disorders is one of the most crucial public health issues. Oral polyamine intake was shown to improve memory performance which is thought to be mediated at least in part via increased autophagy induced in brain cells. In Alzheimer's Disease, T-cells were identified as important...


    pub_type: 杂志文章


    authors: Fischer M,Ruhnau J,Schulze J,Obst D,Flöel A,Vogelgesang A

    更新日期:2020-06-30 00:00:00

  • Long noncoding RNA ARHGAP27P1 inhibits gastric cancer cell proliferation and cell cycle progression through epigenetically regulating p15 and p16.

    abstract::Long noncoding RNAs (lncRNAs) have emerged as important regulators in the development and progression of gastric cancer (GC). ARHGAP27P1 is a pseudogene-derived lncRNA, and it has been found to be associated with GC in our preliminary study, but this association has not been studied further. Herein, we confirmed that ...


    pub_type: 杂志文章


    authors: Zhang G,Xu Y,Zou C,Tang Y,Lu J,Gong Z,Ma G,Zhang W,Jiang P

    更新日期:2019-10-30 00:00:00

  • SNRPB-mediated RNA splicing drives tumor cell proliferation and stemness in hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) is one of the leading malignant diseases worldwide, but therapeutic targets for HCC are lacking. Here, we characterized a significant upregulation of Small Nuclear Ribonucleoprotein Polypeptides B and B1 (SNRPB) in HCC via qRT-PCR, western blotting, tissue microarray and public database ...


    pub_type: 杂志文章


    authors: Zhan YT,Li L,Zeng TT,Zhou NN,Guan XY,Li Y

    更新日期:2020-12-03 00:00:00

  • Downregulation of circFASTKD1 ameliorates myocardial infarction by promoting angiogenesis.

    abstract::Circular RNAs (circRNAs), a novel class of endogenous long non-coding RNAs, have attracted considerable attention due to their closed continuous loop structure and potential clinical value. In this study, we investigated the function of circFASTKD1 in vascular endothelial cells. CircFASTKD1 bound directly to miR-106a ...


    pub_type: 杂志文章


    authors: Gao WQ,Hu XM,Zhang Q,Yang L,Lv XZ,Chen S,Wu P,Duan DW,Lang YH,Ning M,Lai KG,Zhang ZY,Liang B,Bao JY,Wu HD,Li T

    更新日期:2020-12-19 00:00:00

  • The circRNA circIFI30 promotes progression of triple-negative breast cancer and correlates with prognosis.

    abstract::Growing evidence suggests that circRNAs exert a critical role in tumorigenesis and cancer progression. To date, the molecular mechanisms underlying circRNAs in triple-negative breast cancer (TNBC) are still poorly known. Here, circRNA expression profile was investigated by RNA sequencing in TNBC tissues and matched pa...


    pub_type: 杂志文章


    authors: Xing L,Yang R,Wang X,Zheng X,Yang X,Zhang L,Jiang R,Ren G,Chen J

    更新日期:2020-06-04 00:00:00

  • A predictive model for the severity of COVID-19 in elderly patients.

    abstract::Elderly patients with coronavirus disease 2019 (COVID-19) are more likely to develop severe or critical pneumonia, with a high fatality rate. To date, there is no model to predict the severity of COVID-19 in elderly patients. In this study, patients who maintained a non-severe condition and patients who progressed to ...


    pub_type: 杂志文章


    authors: Zeng F,Deng G,Cui Y,Zhang Y,Dai M,Chen L,Han D,Li W,Guo K,Chen X,Shen M,Pan P

    更新日期:2020-11-10 00:00:00

  • Pharmacological blockade of TNFα prevents sarcopenia and prolongs survival in aging mice.

    abstract::Sarcopenia is a hallmark of aging. Inflammation due to increased generation of cytokines such as TNFα, IL-1β and IL-6 has been implicated in the pathogenesis of sarcopenia. In skeletal muscle of C57BL/6 mice from 12 until 28 months of age, we observed a progressive reduction of myofiber cross sectional area, loss of t...


    pub_type: 杂志文章


    authors: Sciorati C,Gamberale R,Monno A,Citterio L,Lanzani C,De Lorenzo R,Ramirez GA,Esposito A,Manunta P,Manfredi AA,Rovere-Querini P

    更新日期:2020-11-26 00:00:00

  • Prevention of diet-induced hepatic steatosis and hepatic insulin resistance by second generation antisense oligonucleotides targeted to the longevity gene mIndy (Slc13a5).

    abstract::Reducing the expression of the Indy (I'm Not Dead Yet) gene in lower organisms extends life span by mechanisms resembling caloric restriction. Similarly, deletion of the mammalian homolog, mIndy (Slc13a5), encoding for a plasma membrane tricarboxylate transporter, protects from aging- and diet-induced adiposity and in...


    pub_type: 杂志文章


    authors: Pesta DH,Perry RJ,Guebre-Egziabher F,Zhang D,Jurczak M,Fischer-Rosinsky A,Daniels MA,Willmes DM,Bhanot S,Bornstein SR,Knauf F,Samuel VT,Shulman GI,Birkenfeld AL

    更新日期:2015-12-01 00:00:00

  • Expression profile-based screening for critical genes reveals S100A4, ACKR3 and CDH1 in docetaxel-resistant prostate cancer cells.

    abstract::Docetaxel is a first-line anticancer drug widely used in the treatment of advanced prostate cancer. However, its therapeutic efficacy is limited by its side effects and the development of chemoresistance by the tumor. Using a gene differential expression microarray, we identified 449 genes differentially expressed in ...


    pub_type: 杂志文章


    authors: Zhu S,Min Z,Qiao X,Chen S,Yang J,Zhang X,Liu X,Ran W,Lv R,Lin Y,Wang J

    更新日期:2019-12-29 00:00:00

  • Clinical significance and prognostic value of TRIM24 expression in esophageal squamous cell carcinoma.

    abstract::Tripartite motif-containing 24 (TRIM24), a member of the transcription intermediary factor 1 family, is defined as a co-regulator with several nuclear receptors, such as RARα. TRIM24 has been reported to be involved in many cancers. In this study, we aimed to investigate the expression pattern and prognostic significa...


    pub_type: 杂志文章


    authors: Chi J,Yang Q,Xie XF,Yang XZ,Zhang MY,Wang HY,Xu GL

    更新日期:2016-09-28 00:00:00

  • MicroRNA-214-3p targets the PLAGL2-MYH9 axis to suppress tumor proliferation and metastasis in human colorectal cancer.

    abstract::Evidence has shown that microRNAs (miRNAs) participate in the progression of CRC. Previous studies have indicated that miR-214-3p is abnormally expressed in various malignant tumors. However, the biological function it plays in CRC and the potential mechanism are unclear. Here, we demonstrated that miR-214-3p was obvi...


    pub_type: 杂志文章


    authors: Zhou Z,Wu L,Liu Z,Zhang X,Han S,Zhao N,Bao H,Yuan W,Chen J,Ji J,Shu X

    更新日期:2020-05-15 00:00:00

  • Silencing KIF14 reverses acquired resistance to sorafenib in hepatocellular carcinoma.

    abstract::For nearly a decade, sorafenib has served as a first-line chemotherapeutic drug for the treatment of hepatocellular carcinoma (HCC), but it displays only limited efficacy against advanced drug-resistant HCC. Regorafenib, the first second-line drug approved for treatment after sorafenib failure, can reverse resistance ...


    pub_type: 杂志文章


    authors: Zhu Q,Ren H,Li X,Qian B,Fan S,Hu F,Xu L,Zhai B

    更新日期:2020-11-16 00:00:00

  • Identification of candidate lncRNAs and circRNAs regulating WNT3/β-catenin signaling in essential hypertension.

    abstract::Mounting evidence suggests that noncoding RNAs (ncRNAs) contribute to the pathogenesis of cardiovascular diseases. However, their role in essential hypertension (EH) is still unclear. We therefore identified differentially expressed long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) in EH patients from a high-...


    pub_type: 杂志文章


    authors: Yin L,Yao J,Deng G,Wang X,Cai W,Shen J

    更新日期:2020-05-11 00:00:00

  • Age-associated deficient recruitment of 53BP1 in G1 cells directs DNA double-strand break repair to BRCA1/CtIP-mediated DNA-end resection.

    abstract::DNA repair mechanisms play a crucial role in maintaining genome integrity. However, the increased frequency of DNA double-strand breaks (DSBs) and genome rearrangements in aged individuals suggests an age-associated DNA repair deficiency. Previous work from our group revealed a delayed firing of the DNA damage respons...


    pub_type: 杂志文章


    authors: Anglada T,Genescà A,Martín M

    更新日期:2020-12-27 00:00:00

  • GSG2 (Haspin) promotes development and progression of bladder cancer through targeting KIF15 (Kinase-12).

    abstract::Bladder cancer is the most commonly diagnosed malignant tumor in urological system worldwide. The relationship between GSG2 and bladder cancer has not been demonstrated and remains unclear. In this study, it was demonstrated that GSG2 was up-regulated in bladder cancer tissues compared with the normal tissues and its ...


    pub_type: 杂志文章


    authors: Chen Y,Fu D,Zhao H,Cheng W,Xu F

    更新日期:2020-05-21 00:00:00

  • Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria.

    abstract::Recently epidemiological studies suggest females lose neuroprotection from neurodegenerative diseases as they go through menopause. It has been hypothesized that this neuroprotection is hormone-dependent. The current study characterized cell signaling molecules downstream of estrogen receptor beta that are known to pl...


    pub_type: 杂志文章


    authors: Evola CM,Hudson TL,Huang L,Corbett AM,Mayes DA

    更新日期:2018-08-31 00:00:00

  • Single-cell RNA sequencing of immune cells in gastric cancer patients.

    abstract::Cancer immunotherapy has achieved positive clinical responses in the treatment of various cancers, including gastric cancer (GC). In this study, we characterized the heterogeneity of T cells isolated from GC patients at the single-cell level using single-cell RNA sequencing. We identified different immune cell subtype...


    pub_type: 杂志文章


    authors: Fu K,Hui B,Wang Q,Lu C,Shi W,Zhang Z,Rong D,Zhang B,Tian Z,Tang W,Cao H,Wang X,Chen Z

    更新日期:2020-02-10 00:00:00

  • Long non-coding RNA EWSAT1 promotes human nasopharyngeal carcinoma cell growth in vitro by targeting miR-326/-330-5p.

    abstract::Long non-coding RNA (lncRNA) Ewing sarcoma associated transcript 1 (EWSAT1) has been identified as an oncogene, and its dysregulation is closed corrected with tumor progression in Ewing sarcoma. Recently, high-through put analysis reveals that EWSAT1 is also highly expressed in human nasopharyngeal carcinoma (NPC). Ho...


    pub_type: 杂志文章


    authors: Song P,Yin SC

    更新日期:2016-11-03 00:00:00

  • DNA methylation perturbations may link altered development and aging in the lung.

    abstract::Fetal perturbations in DNA methylation during lung development may reveal insights into the enduring impacts on adult lung health and disease during aging that have not been explored altogether before. We studied the association between genome-wide DNA-methylation and post-conception age in fetal-lung (n=78, 42 expose...


    pub_type: 杂志文章


    authors: Kachroo P,Morrow JD,Vyhlidal CA,Gaedigk R,Silverman EK,Weiss ST,Tantisira KG,DeMeo DL

    更新日期:2021-01-19 00:00:00

  • Immune profile of the tumor microenvironment and the identification of a four-gene signature for lung adenocarcinoma.

    abstract::The composition and relative abundances of immune cells in the tumor microenvironment are key factors affecting the progression of lung adenocarcinomas (LUADs) and the efficacy of immunotherapy. Using the cancer gene expression dataset from The Cancer Genome Atlas (TCGA) program, we scored stromal and immune cells for...


    pub_type: 杂志文章


    authors: Fan T,Zhu M,Wang L,Liu Y,Tian H,Zheng Y,Tan F,Sun N,Li C,He J

    更新日期:2020-12-09 00:00:00

  • Pyridoxine 5'-phosphate oxidase is correlated with human breast invasive ductal carcinoma development.

    abstract::Pyridoxine 5'-phosphate oxidase (PNPO) is a converting enzyme for an active form of vitamin B6. This study aims to evaluate the biological function and the regulatory mechanism of PNPO in human breast invasive ductal carcinoma (IDC). We unveiled for the first time that PNPO was upregulated in patients with IDC and was...


    pub_type: 杂志文章


    authors: Ren W,Guan W,Zhang J,Wang F,Xu G

    更新日期:2019-04-14 00:00:00

  • NEAT1/miR-200b-3p/SMAD2 axis promotes progression of melanoma.

    abstract::Melanoma is a skin malignancy with a high mutation frequency of genetic alterations. MicroRNA (miR)-200b-3p is involved in various cancers, while in melanoma its bio-function remains unknown. In this study, we found that miR-200b-3p was down-regulated in melanoma tissues and cell lines compared to benign nevus cells. ...


    pub_type: 杂志文章


    authors: Zhou WJ,Wang HY,Zhang J,Dai HY,Yao ZX,Zheng Z,Meng-Yan S,Wu K

    更新日期:2020-11-16 00:00:00

  • PD-1 gene rs10204525 and rs7421861 polymorphisms are associated with increased risk and clinical features of esophageal cancer in a Chinese Han population.

    abstract::Programmed death-1 (PD-1) polymorphisms have been associated with esophageal cancer risk. Here, the aims of this case-control study were to explore whether three PD-1 polymorphisms (rs10204525, rs7421861, and rs36084323) were related with the risk and clinical features of esophageal cancer in Chinese Han subjects (n =...


    pub_type: 杂志文章


    authors: Zang B,Chen C,Zhao JQ

    更新日期:2020-02-21 00:00:00

  • System level characterization of small molecule drugs and their affected long noncoding RNAs.

    abstract::Long noncoding RNAs (lncRNAs) have multiple regulatory roles and are involved in many human diseases. A potential therapeutic strategy based on targeting lncRNAs was recently developed. To gain insight into the global relationship between small molecule drugs and their affected lncRNAs, we constructed a small molecule...


    pub_type: 杂志文章


    authors: Yang H,Jiang Y,Zhang Y,Xu Y,Zhang C,Han J,Su F,Liu X,Mi K,Liu B,Shang D

    更新日期:2019-12-18 00:00:00

  • Association between serum levels of Klotho and inflammatory cytokines in cardiovascular disease: a case-control study.

    abstract::Decrease in soluble anti-aging Klotho protein levels is associated to cardiovascular disease (CVD). Diverse studies have shown a bidirectional relationship between Klotho and inflammation, a risk factor for the development of CVD. In this work we aimed to evaluate the association between Klotho and inflammatory cytoki...


    pub_type: 杂志文章


    authors: Martín-Núñez E,Donate-Correa J,Ferri C,López-Castillo Á,Delgado-Molinos A,Hernández-Carballo C,Pérez-Delgado N,Rodríguez-Ramos S,Cerro-López P,Tagua VG,Mora-Fernández C,Navarro-González JF

    更新日期:2020-01-27 00:00:00

  • A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

    abstract::Increasing evidence has indicated the prognostic value of miR-433 across a series of malignancy types. However, the underlying mechanisms involved in cancer progression haven't been sufficiently elucidated. In the present work, we found that miR-433 was downregulated in CRC tissues and cell lines. Ectopic expression o...


    pub_type: 杂志文章


    authors: Yan L,You WQ,Sheng NQ,Gong JF,Hu LD,Tan GW,Chen HQ,Wang ZG

    更新日期:2018-12-06 00:00:00

  • LAIR-1 suppresses cell growth of ovarian cancer cell via the PI3K-AKT-mTOR pathway.

    abstract::Recently, over-expression of LAIR-1 has been found in some solid cancers, including ovarian cancer. The role of LAIR-1 in cancer progression needs further investigation. In this study, we identified the LAIR-1 cDNA sequence of the ovarian cancer cells HO8910. Using SKOV3 cells, we confirmed the finding from our previo...


    pub_type: 杂志文章


    authors: Liu Y,Ma L,Shangguan F,Zhao X,Wang W,Gao Z,Zhou H,Qu G,Huang Y,An J,Xue J,Yang S,Cao Q

    更新日期:2020-09-05 00:00:00

  • Co-expression network analysis identified hub genes critical to triglyceride and free fatty acid metabolism as key regulators of age-related vascular dysfunction in mice.

    abstract::Background: Aging has often been linked to age-related vascular disorders. The elucidation of the putative genes and pathways underlying vascular aging likely provides useful insights into vascular diseases at advanced ages. Transcriptional regulatory network analysis is the key to describing genetic interactions betw...


    pub_type: 杂志文章


    authors: Li H,Wang X,Lu X,Zhu H,Li S,Duan S,Zhao X,Zhang F,Alterovitz G,Wang F,Li Q,Tian XL,Xu M

    更新日期:2019-09-12 00:00:00