Self-assembly of porphyrin-grafted lipid into nanoparticles encapsulating doxorubicin for synergistic chemo-photodynamic therapy and fluorescence imaging.


:The limited clinical efficacy of monotherapies in the clinic has urged the development of novel combination platforms. Taking advantage of light-triggered photodynamic treatment combined together with the controlled release of nanomedicine, it has been possible to treat cancer without eliciting any adverse effects. However, the challenges imposed by limited drug loading capacity and complex synthesis process of organic nanoparticles (NPs) have seriously impeded advances in chemo-photodynamic combination therapy. In this experiment, we utilize our previously synthesized porphyrin-grafted lipid (PGL) NPs to load highly effective chemotherapeutic drug, doxorubicin (DOX) for synergistic chemo-photodynamic therapy. Methods: A relatively simple and inexpensive rapid injection method was used to prepare porphyrin-grafted lipid (PGL) NPs. The self-assembled PGL NPs were used further to encapsulate DOX via a pH-gradient loading protocol. The self-assembled liposome-like PGL NPs having a hydrophilic core were optimized to load DOX at an encapsulation efficiency (EE) of ~99%. The resultant PGL-DOX NPs were intact, highly stable and importantly these NPs successfully escaped from the endo-lysosomal compartment after laser irradiation to release DOX in the cytosol. The therapeutic efficacy of the aforementioned formulation was validated both in vitro and in vivo. Results: PGL-DOX NPs demonstrated excellent cellular uptake, chemo-photodynamic response, and fluorescence imaging ability in different cell lines. Under laser irradiation, cells treated with a low molar concentration of PGL-DOX NPs reduced cell viability significantly. Moreover, in vivo experiments conducted in a xenograft mouse model further demonstrated the excellent tumor accumulation capability of PGL-DOX NPs driven by the enhanced permeability and retention (EPR) effect. Through fluorescence imaging, the biodistribution of PGL-DOX NPs in tumor and major organs was also easily monitored in real time in vivo. The inherent ability of porphyrin to generate ROS under laser irradiation combined with the cytotoxic effect of the anticancer drug DOX significantly suppressed tumor growth in vivo. Conclusion: In summary, the PGL-DOX NPs combined chemo-photodynamic nanoplatform may serve as a potential candidate for cancer theranostics.






Hameed S,Bhattarai P,Liang X,Zhang N,Xu Y,Chen M,Dai Z




Has Abstract


2018-11-03 00:00:00












  • Active-target T1-weighted MR Imaging of Tiny Hepatic Tumor via RGD Modified Ultra-small Fe3O4 Nanoprobes.

    abstract::Developing ultrasensitive contrast agents for the early detection of malignant tumors in liver is highly demanded. Constructing hepatic tumors specific targeting probes could provide more sensitive imaging information but still faces great challenges. Here we report a novel approach for the synthesis of ultra-small Fe...


    pub_type: 杂志文章


    authors: Jia Z,Song L,Zang F,Song J,Zhang W,Yan C,Xie J,Ma Z,Ma M,Teng G,Gu N,Zhang Y

    更新日期:2016-07-15 00:00:00

  • Quantification of Tumor Vascular Permeability and Blood Volume by Positron Emission Tomography.

    abstract::Purpose: Evans Blue (EB) is an azo dye that binds quantitatively with serum albumin. With an albumin binding, NOTA conjugated truncated Evan's blue (NEB) dye derived PET tracer, we aimed to establish a strategy for evaluating vascular permeability in malignant tumors via non-invasive PET. Experimental design: Sixty-mi...


    pub_type: 杂志文章


    authors: Chen H,Tong X,Lang L,Jacobson O,Yung BC,Yang X,Bai R,Kiesewetter DO,Ma Y,Wu H,Niu G,Chen X

    更新日期:2017-06-14 00:00:00

  • A Combination of DNA-peptide Probes and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS): A Quasi-Targeted Proteomics Approach for Multiplexed MicroRNA Quantification.

    abstract::The distorted and unique expression of microRNAs (miRNAs) in cancer makes them an attractive source of biomarker. There is much evidence indicating that a panel of miRNAs, termed "miRNA fingerprints", is more specific and informative than an individual miRNA as biomarker. Thus, multiplex assays for simultaneous quanti...


    pub_type: 杂志文章


    authors: Xu F,Zhou W,Cao J,Xu Q,Jiang D,Chen Y

    更新日期:2017-07-08 00:00:00

  • Single enzyme loaded nanoparticles for combinational ultrasound-guided focused ultrasound ablation and hypoxia-relieved chemotherapy.

    abstract::Constructing nanosystems that synergistically combine therapeutic and diagnostic features is of great interest to the nanomedicine community but also remains a tremendous challenge. Methods: In this work, we report novel catalytic nanoparticles composed of the enzyme catalase, encapsulated in a polymer shell and surfa...


    pub_type: 杂志文章


    authors: Zhu J,Li Z,Zhang C,Lin L,Cao S,Che H,Shi X,Wang H,van Hest JCM

    更新日期:2019-10-17 00:00:00

  • Triple-Layered pH-Responsive Micelleplexes Loaded with siRNA and Cisplatin Prodrug for NF-Kappa B Targeted Treatment of Metastatic Breast Cancer.

    abstract::The combination of chemotherapy and RNA interference is a promising approach for efficient cancer therapy. However, the success of such a strategy is hampered by the lack of suitable vectors to coordinate small interfering RNA (siRNA) and chemotherapeutic drug into one single platform. We herein report a novel triple-...


    pub_type: 杂志文章


    authors: Yu H,Guo C,Feng B,Liu J,Chen X,Wang D,Teng L,Li Y,Yin Q,Zhang Z,Li Y

    更新日期:2016-01-01 00:00:00

  • The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.

    abstract::The intestinal exporter MRP2 plays an important role in disposition and elimination of a wide range of drugs. Here, we aimed to clarify the impact of circadian clock on intestinal MRP2, and to determine the molecular mechanisms for generation of diurnal MRP2 expression. Methods: The regulatory effects of Bmal1 on inte...


    pub_type: 杂志文章


    authors: Yu F,Zhang T,Zhou C,Xu H,Guo L,Chen M,Wu B

    更新日期:2019-04-13 00:00:00

  • Smooth muscle SIRT1 reprograms endothelial cells to suppress angiogenesis after ischemia.

    abstract::Objective: Vascular smooth muscle cells (VSMCs) undergo the phenotypic changes from contractile to synthetic state during vascular remodeling after ischemia. SIRT1 protects against stress-induced vascular remodeling via maintaining VSMC differentiated phenotype. However, the effect of smooth muscle SIRT1 on the functi...


    pub_type: 杂志文章


    authors: Dou YQ,Kong P,Li CL,Sun HX,Li WW,Yu Y,Nie L,Zhao LL,Miao SB,Li XK,Dong C,Zhang JW,Liu Y,Huo XX,Chi K,Gao X,Zhang N,Weng L,Yang H,Zhang F,Han M

    更新日期:2020-01-01 00:00:00

  • Bright Polymer Dots Tracking Stem Cell Engraftment and Migration to Injured Mouse Liver.

    abstract::Stem cell therapy holds promise for treatment of intractable diseases and injured organs. For clinical translation, it is pivotal to understand the homing, engraftment, and differentiation processes of stem cells in a living body. Here we report near-infrared (NIR) fluorescent semiconductor polymer dots (Pdots) for br...


    pub_type: 杂志文章


    authors: Chen D,Li Q,Meng Z,Guo L,Tang Y,Liu Z,Yin S,Qin W,Yuan Z,Zhang X,Wu C

    更新日期:2017-04-10 00:00:00

  • Nanoscale microenvironment engineering based on layer-by-layer self-assembly to regulate hair follicle stem cell fate for regenerative medicine.

    abstract::Hair regenerative medicine, a promising strategy for the treatment of hair loss, will likely involve the transplantation of autologous hair follicular stem cells (HFSCs) and dermal papilla cells (DPCs) into regions of hair loss. Cyclic hair regeneration results from the periodic partial activation of HFSCs. However, p...


    pub_type: 杂志文章


    authors: Chen P,Miao Y,Zhang F,Huang J,Chen Y,Fan Z,Yang L,Wang J,Hu Z

    更新日期:2020-09-22 00:00:00

  • MicroRNAs: New Biomarkers for Diagnosis, Prognosis, Therapy Prediction and Therapeutic Tools for Breast Cancer.

    abstract::Dysregulation of microRNAs (miRNAs) is involved in the initiation and progression of several human cancers, including breast cancer (BC), as strong evidence has been found that miRNAs can act as oncogenes or tumor suppressor genes. This review presents the state of the art on the role of miRNAs in the diagnosis, progn...


    pub_type: 杂志文章,评审


    authors: Bertoli G,Cava C,Castiglioni I

    更新日期:2015-07-13 00:00:00

  • Fatty acid-induced CD36 expression via O-GlcNAcylation drives gastric cancer metastasis.

    abstract::Metastasis is the primary cause of death in patients with advanced cancer. Recently, a high-fat diet was shown to specifically promote the metastatic potential of specific cancer cells in a CD36-dependent manner. However, the molecular basis of the fatty acid (FA)-induced upregulation of CD36 has remained unclear. Met...


    pub_type: 杂志文章


    authors: Jiang M,Wu N,Xu B,Chu Y,Li X,Su S,Chen D,Li W,Shi Y,Gao X,Zhang H,Zhang Z,Du W,Nie Y,Liang J,Fan D

    更新日期:2019-07-09 00:00:00

  • Glioma Dual-Targeting Nanohybrid Protein Toxin Constructed by Intein-Mediated Site-Specific Ligation for Multistage Booster Delivery.

    abstract::Malignant glioma is one of the most untreatable cancers because of the formidable blood-brain barrier (BBB), through which few therapeutics can penetrate and reach the tumors. Biologics have been booming in cancer therapy in the past two decades, but their application in brain tumor has long been ignored due to the im...


    pub_type: 杂志文章


    authors: Chen Y,Zhang M,Jin H,Li D,Xu F,Wu A,Wang J,Huang Y

    更新日期:2017-08-15 00:00:00

  • Membrane Radiolabelling of Exosomes for Comparative Biodistribution Analysis in Immunocompetent and Immunodeficient Mice - A Novel and Universal Approach.

    abstract::Extracellular vesicles, in particular exosomes, have recently gained interest as novel drug delivery vectors due to their biological origin and inherent intercellular biomolecule delivery capability. An in-depth knowledge of their in vivo biodistribution is therefore essential. This work aimed to develop a novel, reli...


    pub_type: 杂志文章


    authors: Faruqu FN,Wang JT,Xu L,McNickle L,Chong EM,Walters A,Gurney M,Clayton A,Smyth LA,Hider R,Sosabowski J,Al-Jamal KT

    更新日期:2019-02-28 00:00:00

  • Role of Extracellular Vesicles in Viral and Bacterial Infections: Pathogenesis, Diagnostics, and Therapeutics.

    abstract::Extracellular vesicles (EVs), or exosomes, are nanovesicles of endocytic origin that carry host and pathogen-derived protein, nucleic acid, and lipid cargos. They are secreted by most cell types and play important roles in normal cell-to-cell communications but can also spread pathogen- and host-derived molecules duri...


    pub_type: 杂志文章,评审


    authors: Rodrigues M,Fan J,Lyon C,Wan M,Hu Y

    更新日期:2018-04-09 00:00:00

  • Soyasaponin II protects against acute liver failure through diminishing YB-1 phosphorylation and Nlrp3-inflammasome priming in mice.

    abstract::Acute liver failure is characterized by the rapid development of liver dysfunction and remarkably high mortality. Accumulating evidence suggests that soyasaponin possesses potential anti-inflammatory activities. Here, we aimed to investigate the potential role of soyasaponin II in acute liver failure and establish the...


    pub_type: 杂志文章


    authors: Wang F,Gong S,Wang T,Li L,Luo H,Wang J,Huang C,Zhou H,Chen G,Liu Z,Zhang Q,Jiang Y,Chen P

    更新日期:2020-02-03 00:00:00

  • Roles of long non-coding RNAs and emerging RNA-binding proteins in innate antiviral responses.

    abstract::The diseases caused by viruses posed a great challenge to human health, the development of which was driven by the imbalanced host immune response. Host innate immunity is an evolutionary old defense system that is critical for the elimination of the virus. The overactive innate immune response also leads to inflammat...


    pub_type: 杂志文章,评审


    authors: Wang Y,Wang Y,Luo W,Song X,Huang L,Xiao J,Jin F,Ren Z,Wang Y

    更新日期:2020-07-23 00:00:00

  • Pretargeted molecular imaging and radioimmunotherapy.

    abstract::Pretargeting is a multi-step process that first has an unlabeled bispecific antibody (bsMAb) localize within a tumor by virtue of its anti-tumor binding site(s) before administering a small, fast-clearing radiolabeled compound that then attaches to the other portion of the bsMAb. The compound's rapid clearance signifi...


    pub_type: 杂志文章


    authors: Goldenberg DM,Chang CH,Rossi EA,J W,McBride,Sharkey RM

    更新日期:2012-01-01 00:00:00

  • Assessing Therapeutic Potential of Magnetic Mesoporous Nanoassemblies for Chemo-Resistant Tumors.

    abstract::Smart drug delivery system with strategic drug distribution is the future state-of-the-art treatment for any malignancy. To investigate therapeutic potential of such nanoparticle mediated delivery system, we examined the efficacy of dual drug-loaded, pH and thermo liable lipid coated mesoporous iron oxide-based magnet...


    pub_type: 杂志文章


    authors: Pradhan L,Thakur B,Srivastava R,Ray P,Bahadur D

    更新日期:2016-06-18 00:00:00

  • RUNX2 overexpression and PTEN haploinsufficiency cooperate to promote CXCR7 expression and cellular trafficking, AKT hyperactivation and prostate tumorigenesis.

    abstract::Rationale: The overall success rate of prostate cancer (PCa) diagnosis and therapy has been improved over the years. However, genomic and phenotypic heterogeneity remains a major challenge for effective detection and treatment of PCa. Efforts to better classify PCa into functional subtypes and elucidate the molecular ...


    pub_type: 杂志文章


    authors: Bai Y,Yang Y,Yan Y,Zhong J,Blee AM,Pan Y,Ma T,Karnes RJ,Jimenez R,Xu W,Huang H

    更新日期:2019-05-24 00:00:00

  • Inorganic Kernel-Reconstituted Lipoprotein Biomimetic Nanovehicles Enable Efficient Targeting "Trojan Horse" Delivery of STAT3-Decoy Oligonucleotide for Overcoming TRAIL Resistance.

    abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in a variety of tumor cells, but not most normal cells. Nevertheless, its therapeutic potential is limited due to the frequent occurrence of resistance in tumor cells, especially hepatocellular carcinoma cell lines. Theref...


    pub_type: 杂志文章


    authors: Shi K,Xue J,Fang Y,Bi H,Gao S,Yang D,Lu A,Li Y,Chen Y,Ke L

    更新日期:2017-10-12 00:00:00

  • Oleic acid-induced NOX4 is dependent on ANGPTL4 expression to promote human colorectal cancer metastasis.

    abstract::Background: Colorectal cancer (CRC) progression and related mortality are highly associated with metabolic disorders. However, the molecular mechanism involved in the regulation of hyperlipidemia-associated CRC metastasis remains unclear. This study aimed to investigate the effects of angiopoietin-like 4 (ANGPTL4) on ...


    pub_type: 杂志文章


    authors: Shen CJ,Chang KY,Lin BW,Lin WT,Su CM,Tsai JP,Liao YH,Hung LY,Chang WC,Chen BK

    更新日期:2020-05-30 00:00:00

  • Dendrimer- and copolymer-based nanoparticles for magnetic resonance cancer theranostics.

    abstract::Cancer theranostics is one of the most important approaches for detecting and treating patients at an early stage. To develop such a technique, accurate detection, specific targeting, and controlled delivery are the key components. Various kinds of nanoparticles have been proposed and demonstrated as potential nanoveh...


    pub_type: 杂志文章,评审


    authors: Ray S,Li Z,Hsu CH,Hwang LP,Lin YC,Chou PT,Lin YY

    更新日期:2018-11-29 00:00:00

  • MicroRNA-193b-3p regulates chondrogenesis and chondrocyte metabolism by targeting HDAC3.

    abstract::Histone deacetylase 3 (HDAC3) plays a pivotal role in the repression of cartilage-specific gene expression in human chondrocytes. The aim of this study was to determine whether microRNA-193b-3p (miR-193b-3p) regulates the expression of HDAC3 during chondrogenesis and chondrocyte metabolism. Methods: miR-193b-3p expres...


    pub_type: 杂志文章


    authors: Meng F,Li Z,Zhang Z,Yang Z,Kang Y,Zhao X,Long D,Hu S,Gu M,He S,Wu P,Chang Z,He A,Liao W

    更新日期:2018-04-15 00:00:00

  • Endothelial Klf2-Foxp1-TGFβ signal mediates the inhibitory effects of simvastatin on maladaptive cardiac remodeling.

    abstract::Aims: Pathological cardiac fibrosis and hypertrophy are common features of left ventricular remodeling that often progress to heart failure (HF). Endothelial cells (ECs) are the most abundant non-myocyte cells in adult mouse heart. Simvastatin, a strong inducer of Krüppel-like Factor 2 (Klf2) in ECs, ameliorates press...


    pub_type: 杂志文章


    authors: Li H,Wang Y,Liu J,Chen X,Duan Y,Wang X,Shen Y,Kuang Y,Zhuang T,Tomlinson B,Chan P,Yu Z,Cheng Y,Zhang L,Liu Z,Zhang Y,Zhao Z,Zhang Q,Liu J

    更新日期:2021-01-01 00:00:00

  • Acidic Microenvironment Up-Regulates Exosomal miR-21 and miR-10b in Early-Stage Hepatocellular Carcinoma to Promote Cancer Cell Proliferation and Metastasis.

    abstract::Rationale: The incidence of hepatocellular carcinoma is rising worldwide. It is predicted that nearly half of the early-stage hepatocellular carcinoma (E-HCC) patients will develop recurrence. Dysregulated pH, a hallmark of E-HCC, is correlated with poor prognosis. The acidic microenvironment has been shown to promote...


    pub_type: 杂志文章


    authors: Tian XP,Wang CY,Jin XH,Li M,Wang FW,Huang WJ,Yun JP,Xu RH,Cai QQ,Xie D

    更新日期:2019-03-16 00:00:00

  • Theranostics in immuno-oncology using nanobody derivatives.

    abstract::Targeted therapy and immunotherapy have become mainstream in cancer treatment. However, only patient subsets benefit from these expensive therapies, and often responses are short-lived or coincide with side effects. A growing modality in precision oncology is the development of theranostics, as this enables patient se...


    pub_type: 杂志文章,评审


    authors: Lecocq Q,De Vlaeminck Y,Hanssens H,D'Huyvetter M,Raes G,Goyvaerts C,Keyaerts M,Devoogdt N,Breckpot K

    更新日期:2019-10-15 00:00:00

  • The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes.

    abstract::Exosomes play critical roles in intercellular communication in both nearby and distant cells in individuals and organs. Polymerase I and transcript release factor (PTRF), also known as Cavin1, has previously been described as a critical factor in caveola formation, and aberrant PTRF expression has been reported in var...


    pub_type: 杂志文章


    authors: Huang K,Fang C,Yi K,Liu X,Qi H,Tan Y,Zhou J,Li Y,Liu M,Zhang Y,Yang J,Zhang J,Li M,Kang C

    更新日期:2018-02-07 00:00:00

  • Applications of magnetic microbubbles for theranostics.

    abstract::Compared with other diagnostic methods, ultrasound is proven to be a safe, simple, non-invasive and cost-effective imaging technique, but the resolution is not comparable to that of magnetic resonance imaging (MRI). Contrast-enhanced ultrasound employing microbubbles can gain a better resolution and is now widely used...


    pub_type: 杂志文章


    authors: Cai X,Yang F,Gu N

    更新日期:2012-01-01 00:00:00

  • Mdig promotes oncogenic gene expression through antagonizing repressive histone methylation markers.

    abstract::The mineral dust-induced gene (mdig) is overexpressed in a number of human cancers, suggesting critical roles of this gene played on the pathogenesis of cancers. Unlike several other JmjC-domain containing proteins that exhibit histone demethylase activity, it remains enigmatic whether mdig is involved in the demethyl...


    pub_type: 杂志文章


    authors: Zhang Q,Thakur C,Fu Y,Bi Z,Wadgaonkar P,Xu L,Liu Z,Liu W,Wang J,Kidder BL,Chen F

    更新日期:2020-01-01 00:00:00

  • Critical reviews of immunotheranostics.

    abstract::Immunity is the most critical and well-regulated protection to the body. Immunity is implicated in a wide range of diseases and serves as the foundation for immunotherapy. Immunotheranostics is the idea of improving immunotherapy through the organic integration of therapeutic, diagnostic, and screening technologies. T...


    pub_type: 社论


    authors: Chen X,Chen M

    更新日期:2020-06-11 00:00:00