Current and Emerging Technologies for Probing Molecular Signatures of Traumatic Brain Injury.

Abstract:

:Traumatic brain injury (TBI) is understood as an interplay between the initial injury, subsequent secondary injuries, and a complex host response all of which are highly heterogeneous. An understanding of the underlying biology suggests a number of windows where mechanistically inspired interventions could be targeted. Unfortunately, biologically plausible therapies have to-date failed to translate into clinical practice. While a number of stereotypical pathways are now understood to be involved, current clinical characterization is too crude for it to be possible to characterize the biological phenotype in a truly mechanistically meaningful way. In this review, we examine current and emerging technologies for fuller biochemical characterization by the simultaneous measurement of multiple, diverse biomarkers. We describe how clinically available techniques such as cerebral microdialysis can be leveraged to give mechanistic insights into TBI pathobiology and how multiplex proteomic and metabolomic techniques can give a more complete description of the underlying biology. We also describe spatially resolved label-free multiplex techniques capable of probing structural differences in chemical signatures. Finally, we touch on the bioinformatics challenges that result from the acquisition of such large amounts of chemical data in the search for a more mechanistically complete description of the TBI phenotype.

journal_name

Front Neurol

journal_title

Frontiers in neurology

authors

Ercole A,Magnoni S,Vegliante G,Pastorelli R,Surmacki J,Bohndiek SE,Zanier ER

doi

10.3389/fneur.2017.00450

subject

Has Abstract

pub_date

2017-08-30 00:00:00

pages

450

issn

1664-2295

journal_volume

8

pub_type

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