Abstract：
:Daptomycin is a lipopeptide antibiotic approved for use against Gram-positive organisms, including highly resistant species. A number of studies have suggested that daptomycin kills bacteria by membrane permeabilization and depolarization. Recently a model membrane system consisting of 1,2-dimyristoyl-sn-glycero-3-phosphocholine and 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) in a 1:1 ratio and the ionophore CCCP was proposed as a simple model to investigate the mode of action of daptomycin and resistance mechanisms at a molecular level. This study investigates how this model depends on the composition of the membrane and the role of CCCP. Results obtained from a fluorescence assay using pyranine show that daptomycin causes leakage in liposomes of limited stability and that CCCP promotes this leakage. A different model membrane system used here, which relies on ion selective dyes such as 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-, tetrakis[(acetyloxy)methyl] ester (PBFI), and 4,4'-[1,4,10-trioxa-7,13-diazacyclopentadecane-7,13-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-, tetraammonium salt (SBFI), is a more robust alternative. Findings based on this newer model suggest that daptomycin is selective for potassium.
journal_name
ACS Infect Disjournal_title
ACS infectious diseasesauthors
Zhang J,Scoten K,Straus SKdoi
10.1021/acsinfecdis.6b00152subject
Has Abstractpub_date
2016-10-14 00:00:00pages
682-687issue
10issn
2373-8227journal_volume
2pub_type
杂志文章abstract：:Enterohemorrhagic Escherichia coli (EHEC) is the causative agent of severe diarrheal disease in humans. Cattle are the natural reservoir of EHEC, and approximately 75% of EHEC infections in humans stem from bovine products. Many common bacterial pathogens, including EHEC, rely on chemical communication systems, such a...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.0c00654
更新日期：2020-12-11 00:00:00
abstract：:In this study, we describe a simple and rapid antibacterial susceptibility testing (AST) method for Staphylococcus aureus called S. aureus specific fluorescence resonance energy transfer (FRET) probe-based AST (SF-AST), which is based on an S. aureus specific FRET probe (SF probe) that detects micrococcal nuclease (MN...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.9b00260
更新日期：2020-02-14 00:00:00
abstract：:Bacterial sliding clamps control the access of DNA polymerases to the replication fork and are appealing targets for antibacterial drug development. It is therefore essential to decipher the polymerase-clamp binding mode across various bacterial species. Here, two residues of the E. coli clamp binding pocket, EcS346 a...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.9b00089
更新日期：2019-06-14 00:00:00
abstract：:Computer-aided screening of antimicrobial peptides (AMPs) is a promising approach for discovering novel therapies against multidrug-resistant bacterial infections. Here, we functionally and structurally characterized an Escherichia coli-derived AMP (EcDBS1R5) previously designed through pattern identification [α-helic...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.8b00219
更新日期：2018-12-14 00:00:00
abstract：:The enzyme N-myristoyltransferase (NMT) catalyzes the essential fatty acylation of substrate proteins with myristic acid in eukaryotes and is a validated drug target in the parasite Trypanosoma brucei, the causative agent of African trypanosomiasis (sleeping sickness). N-Myristoylation typically mediates membrane loca...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.6b00034
更新日期：2016-06-10 00:00:00
abstract：:Nucleic acid polymers (NAPs) are broad spectrum antiviral agents whose antiviral activity in hepatitis B virus (HBV) infection is derived from their ability to block the release of the hepatitis B virus surface antigen (HBsAg). This pharmacological activity blocks replenishment of HBsAg in the circulation, allowing ho...
journal_title：ACS infectious diseases
pub_type： 杂志文章,评审
doi：10.1021/acsinfecdis.8b00156
更新日期：2019-05-10 00:00:00
abstract：:The envelope glycoprotein (Env) enables HIV-1 cell entry through fusion of host-cell and viral membranes induced by the transmembrane subunit gp41. Antibodies targeting the C-terminal sequence of the membrane-proximal external region (C-MPER) block the fusogenic activity of gp41 and achieve neutralization of divergent...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.0c00243
更新日期：2020-08-14 00:00:00
abstract：:Recent epidemiological studies have revealed that Porphyromonas gingivalis, a major pathogen in periodontal disease, is associated with the development of oral squamous cell carcinoma (OSCC). However, the underlying mechanisms induced by P. gingivalis have not been well-defined. We aimed to determine the role of P. gi...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.9b00032
更新日期：2019-08-09 00:00:00
abstract：:Decreasing the therapeutic pipeline for vancomycin-resistant Enterococci (VRE) calls for novel strategies to enhance our antibacterial arsenal. Herein, we investigated the potential applications of surface localized antimicrobial display (SLAY)-derived cationic peptides in the fight against VanA operon mediated vancom...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.9b00164
更新日期：2020-05-08 00:00:00
abstract：:A method for the fast isolation, propagation, and characterization of very low count bacteriophages active against pathogenic bacterial strains is described in this study. Bacteriophages with a count of 102 phage particles were dynamically adhered from the maximum 10 mL blood plasma sample onto the nanostructured part...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.0c00358
更新日期：2020-10-09 00:00:00
abstract：:RNA-dependent RNA polymerases (RdRPs) from nonsegmented negative strand (NNS) RNA viruses perform both mRNA transcription and genome replication, and these activities are regulated by their interactions with RNA and other accessory proteins within the ribonucleoprotein (RNP) complex. Detailed biochemical characterizat...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.0c00554
更新日期：2020-10-09 00:00:00
abstract：:Biogenic and biomimetic therapeutics are a relatively new class of systems that are of physiological origin and/or take advantage of natural pathways or aim at mimicking these to improve selective interaction with target tissue. The number of biogenic and bioengineered avenues for drug therapy and diagnostics has mult...
journal_title：ACS infectious diseases
pub_type： 杂志文章,评审
doi：10.1021/acsinfecdis.8b00030
更新日期：2018-06-08 00:00:00
abstract：:Poor patient adherence to antiretroviral medication represents a major obstacle for managing disease and reducing rates of new HIV infections. The measurement of patient drug levels is the most objective method of determining adherence. Tenofovir and tenofovir diphosphate are metabolites of some of the most common HIV...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.0c00010
更新日期：2020-07-10 00:00:00
abstract：:Individuals who exhibit long-term HIV suppression and CD4 T-cell preservation without antiretroviral therapy are of great interest for HIV research. There is currently no robust method for rapid identification of these "HIV controller" subjects; however, HLA-B*57 (human leukocyte antigen (major histocompatibility comp...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.7b00042
更新日期：2017-12-08 00:00:00
abstract：:Conventional antibiotics are not effective in treating infections caused by drug-resistant or persistent nongrowing bacteria, creating a dire need for the development of new antibiotics. We report that the small molecule nTZDpa, previously characterized as a nonthiazolidinedione peroxisome proliferator-activated recep...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.8b00161
更新日期：2018-11-09 00:00:00
abstract：:Clostridioides difficile (C. difficile) infection (CDI) is the primary cause of nosocomial antibiotic-associated diarrhea, with high recurrence rates following initial antibiotic treatment regimens. Restoration of the host gut microbiome through probiotic therapy is under investigation to reduce recurrence. Current in...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.9b00415
更新日期：2020-05-08 00:00:00
abstract：:Emerging β-lactamase-mediated resistance is threatening the clinical utility of the single most prominent class of antibacterial agents used in medicine, the β-lactams. The diazabicyclooctane avibactam is able to inhibit a wider range of serine β-lactamases than has been previously observed with β-lactamase inhibitors...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.5b00007
更新日期：2015-04-10 00:00:00
abstract：:Dysentery is a major health threat that dramatically impacts childhood morbidity and mortality in developing countries. Various pathogenic agents cause dysentery, such as Shigella spp. and Escherichia coli, which are very closely related if not identical species. Sensitive and precise detection and identification of t...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.9b00199
更新日期：2019-11-08 00:00:00
abstract：:Natural product antibiotics have played an essential role in the treatment of bacterial infection in addition to serving as useful tools to explore the intricate biology of bacteria. Our current arsenal of antibiotics operate through the inhibition of well-defined bacterial targets critical for replication and growth....
journal_title：ACS infectious diseases
pub_type： 杂志文章,评审
doi：10.1021/acsinfecdis.9b00413
更新日期：2020-04-10 00:00:00
abstract：:Daptomycin is a calcium-dependent lipopeptide antibiotic that is used clinically against various Gram-positive pathogens. It acts on bacterial cell membranes, whose susceptibility varies with the content of phosphatidylglycerol (PG). Some studies have reported that daptomycin permeabilizes and depolarizes bacterial ce...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.7b00138
更新日期：2017-11-10 00:00:00
abstract：:Candida albicans is an opportunistic pathogenic yeast and is responsible for candidiasis. It readily colonizes host tissues and implant devices, and forms biofilms, which play an important role in pathogenesis and drug resistance. In this study, the antibiofilm, antihyphal, and antivirulence activities of nepodin, iso...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.9b00033
更新日期：2019-07-12 00:00:00
abstract：:Glycopeptide antibiotics (GPAs) are a key weapon in the fight against drug resistant bacteria, with vancomycin still a mainstream therapy against serious Gram-positive infections more than 50 years after it was first introduced. New, more potent semisynthetic derivatives that have entered the clinic, such as dalbavanc...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.7b00258
更新日期：2018-05-11 00:00:00
abstract：:Streptococcus mutans ( S. mutans) is a Gram-positive human pathogen that is one of the major contributors to dental caries, a condition with an economic cost of over $100 billion per year in the United States. S. mutans secretes a 21-amino-acid peptide termed the competence stimulating peptide (21-CSP) to assess its p...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.8b00115
更新日期：2018-09-14 00:00:00
abstract：:Pandemics such as influenza, smallpox, and plague have caused the loss of hundreds of millions of lives and have occurred for many centuries. Fortunately, they have been largely eliminated by the use of vaccinations and drugs. More recently, Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (M...
journal_title：ACS infectious diseases
pub_type： 历史文章,杂志文章,评审
doi：10.1021/acsinfecdis.0c00291
更新日期：2020-07-10 00:00:00
abstract：:Many viruses, including the hepatitis C virus (HCV), are dependent on the host RNA silencing pathway for replication. In this study, we screened small molecule probes, previously reported to disrupt loading of the RNA-induced silencing complex (RISC), including 6-hydroxydopamine (6-OHDA), suramin (SUR), and aurintrica...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.6b00098
更新日期：2016-11-11 00:00:00
abstract：:The α-Gal antigen [Galα(1,3)Galβ(1,4)GlcNAcα] is an immunodominant epitope displayed by infective trypomastigote forms of Trypanosoma cruzi, the causative agent of Chagas disease. A virus-like particle displaying a high density of α-Gal was found to be a superior reagent for the ELISA-based serological diagnosis of Ch...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.6b00114
更新日期：2016-12-09 00:00:00
abstract：:Protein S-palmitoylation is an important post-translational modification (PTM) in blood stages of the malaria parasite, Plasmodium falciparum. S-palmitoylation refers to reversible covalent modification of cysteine residues of proteins by saturated fatty acids. In vivo, palmitoylation is regulated by concerted activit...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.9b00321
更新日期：2020-03-13 00:00:00
abstract：:The genomes of diverse mycobacterial species encode multiple proteins with the canonical l,d-transpeptidase (Ldt) sequence motif. The reason for this apparent redundancy is not well understood, but evidence suggests paralogous Ldts may serve niche roles in maintaining and/or remodeling mycobacterial peptidoglycan. We ...
journal_title：ACS infectious diseases
pub_type： 信件
doi：10.1021/acsinfecdis.9b00234
更新日期：2019-12-13 00:00:00
abstract：:Quaternary ammonium compounds (QACs) have historically served as a first line of defense against pathogenic bacteria. Recent reports have shown that QAC resistance is increasing at an alarming rate, especially among methicillin-resistant Staphylococcus aureus (MRSA), and preliminary work has suggested that the number ...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.5b00032
更新日期：2015-07-10 00:00:00
abstract：:The fusion between the viral and the target cell membrane is a crucial step in the life cycle of enveloped viruses. The blocking of this process is a well-known therapeutic approach that led to the development of the fusion inhibitor peptide enfuvirtide, clinically used against human immunodeficiency virus (HIV) type ...
journal_title：ACS infectious diseases
pub_type： 杂志文章
doi：10.1021/acsinfecdis.8b00321
更新日期：2019-04-12 00:00:00
