当前位置： SCI文献检索 > ACS Infectious Diseases期刊下所有文献 > Biochemical Characterization of Respiratory Syncytial Virus RNA-Dependent RNA Polymerase Complex.

Biochemical Characterization of Respiratory Syncytial Virus RNA-Dependent RNA Polymerase Complex.

Abstract：

:RNA-dependent RNA polymerases (RdRPs) from nonsegmented negative strand (NNS) RNA viruses perform both mRNA transcription and genome replication, and these activities are regulated by their interactions with RNA and other accessory proteins within the ribonucleoprotein (RNP) complex. Detailed biochemical characterization of these enzymatic activities and their regulation is essential for understanding the life cycles of many pathogenic RNA viruses and for antiviral drug discovery. We developed biochemical and biophysical kinetic methods to study the RNA synthesis and RNA binding activities of respiratory syncytial virus (RSV) L/P RdRP. We determined that the intact L protein is essential for RdRP activity, and in truncated L protein constructs, RdRP activity is abrogated due to their deficiency in RNA template binding. These results are in agreement with the observation of an RNA template-binding tunnel at the interface of RdRP and capping domains in RSV and vesicular stomatitis virus (VSV) L protein cryo-EM structures. We also describe nonradiometric assays for measuring RNA binding and RNA polymerization activity of RSV RdRP, which are amenable to compound screening and profiling.

journal_name

ACS Infect Dis

journal_title

ACS infectious diseases

authors

Balakrishnan A,Price E,Luu C,Shaul J,Wartchow C,Cantwell J,Vo T,DiDonato M,Spraggon G,Hekmat-Nejad M

doi

10.1021/acsinfecdis.0c00554

subject

Has Abstract

pub_date

2020-10-09 00:00:00

pages

2800-2811

issue

10

issn

2373-8227

journal_volume

6

pub_type

杂志文章

相关文献

ACS Infectious Diseases文献大全
  • Proof-of-Concept Rapid Diagnostic Test for Onchocerciasis: Exploring Peptide Biomarkers and the Use of Gold Nanoshells as Reporter Nanoparticles.

    abstract：:Three O. volvulus immunogenic peptide sequences recently discovered by peptide microarray were adapted to a lateral flow assay (LFA). The LFA employs gold nanoshells as novel high-contrast reporter nanoparticles and detects a serological response against the 3 peptides, found in OvOC9384, OvOC198, and OvOC5528, respec...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.8b00031

    authors： Gonzalez-Moa MJ,Van Dorst B,Lagatie O,Verheyen A,Stuyver L,Biamonte MA

    更新日期：2018-06-08 00:00:00

  • Efforts Aimed To Reduce Attrition in Antimalarial Drug Discovery: A Systematic Evaluation of the Current Antimalarial Targets Portfolio.

    abstract：:Malaria remains a major global health problem. In 2015 alone, more than 200 million cases of malaria were reported, and more than 400,000 deaths occurred. Since 2010, emerging resistance to current front-line ACTs (artemisinin combination therapies) has been detected in endemic countries. Therefore, there is an urgenc...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.7b00211

    authors： Chaparro MJ,Calderón F,Castañeda P,Fernández-Alvaro E,Gabarró R,Gamo FJ,Gómez-Lorenzo MG,Martín J,Fernández E

    更新日期：2018-04-13 00:00:00

  • Modification at the 2'-Position of the 4,5-Series of 2-Deoxystreptamine Aminoglycoside Antibiotics To Resist Aminoglycoside Modifying Enzymes and Increase Ribosomal Target Selectivity.

    abstract：:A series of derivatives of the 4,5-disubstituted class of 2-deoxystreptamine aminoglycoside antibiotics neomycin, paromomycin, and ribostamycin was prepared and assayed for (i) their ability to inhibit protein synthesis by bacterial ribosomes and by engineered bacterial ribosomes carrying eukaryotic decoding A sites, ...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00128

    authors： Sati GC,Sarpe VA,Furukawa T,Mondal S,Mantovani M,Hobbie SN,Vasella A,Böttger EC,Crich D

    更新日期：2019-10-11 00:00:00

  • Rapid Isolation, Propagation, and Online Analysis of a Small Number of Therapeutic Staphylococcal Bacteriophages from a Complex Matrix.

    abstract：:A method for the fast isolation, propagation, and characterization of very low count bacteriophages active against pathogenic bacterial strains is described in this study. Bacteriophages with a count of 102 phage particles were dynamically adhered from the maximum 10 mL blood plasma sample onto the nanostructured part...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00358

    authors： Horká M,Šalplachta J,Karásek P,Růžička F,Štveráková D,Pantůček R,Roth M

    更新日期：2020-10-09 00:00:00

  • Enterovirus D68 Antivirals: Past, Present, and Future.

    abstract：:Enterovirus D68 (EV-D68) is an RNA virus that causes respiratory illnesses mainly in children. In severe cases, it can lead to neurological complications such as acute flaccid myelitis (AFM). EV-D68 belongs to the enterovirus genera of the Picornaviridae family, which also includes many other significant human pathoge...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00120

    authors： Hu Y,Musharrafieh R,Zheng M,Wang J

    更新日期：2020-07-10 00:00:00

  • New Amidated 3,6-Diphenylated Imidazopyridazines with Potent Antiplasmodium Activity Are Dual Inhibitors of Plasmodium Phosphatidylinositol-4-kinase and cGMP-Dependent Protein Kinase.

    abstract：:Recent studies on 3,6-diphenylated imidazopyridazines have demonstrated impressive in vitro activity and in vivo efficacy in mouse models of malaria infection. Herein, we report the synthesis and antiplasmodium evaluation of a new series of amidated analogues and demonstrate that these compounds potently inhibit Plasm...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00481

    authors： Cheuka PM,Centani L,Arendse LB,Fienberg S,Wambua L,Renga SS,Dziwornu GA,Kumar M,Lawrence N,Taylor D,Wittlin S,Coertzen D,Reader J,van der Watt M,Birkholtz LM,Chibale K

    更新日期：2021-01-08 00:00:00

  • Nerve Growth Factor Confers Neuroprotection against Colistin-Induced Peripheral Neurotoxicity.

    abstract：:Neurotoxicity is an unwanted side effect for patients when receiving parenteral colistin therapy. The development of effective neuroprotective agents that can be coadministered during colistin therapy remains a priority area in antimicrobial chemotherapy. The present study aimed to investigate the protective effect of...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00107

    authors： Dai C,Xiong J,Wang Y,Shen J,Velkov T,Xiao X

    更新日期：2020-06-12 00:00:00

  • The Bare Essentials of Antibiotic Target Validation.

    abstract：:The convergence of competitive fitness experiments and phenotypic screening would seem to be an auspicious beginning for validation of an antibacterial target. IMPDH was already identified an essential protein in Mycobacterium tuberculosis when not one, but two, groups discovered inhibitors with promising antitubercul...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.6b00185

    authors： Hedstrom L

    更新日期：2017-01-13 00:00:00

  • LRRK2 in Infection: Friend or Foe?

    abstract：:In the field of Parkinson's disease (PD) research, leucine-rich repeat kinase 2 (LRRK2) remains one of the most enigmatic kinases. LRRK2 pathogenic mutations result in increased kinase activity, making LRRK2 an attractive therapeutic target for PD. For over 10 years, the identification of a bona fide substrate and a p...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00051

    authors： Herbst S,Gutierrez MG

    更新日期：2019-06-14 00:00:00

  • Investigational Studies on a Hit Compound Cyclopropane-Carboxylic Acid Derivative Targeting O-Acetylserine Sulfhydrylase as a Colistin Adjuvant.

    abstract：:Antibacterial adjuvants are of great significance, since they allow the therapeutic dose of conventional antibiotics to be lowered and reduce the insurgence of antibiotic resistance. Herein, we report that an O-acetylserine sulfhydrylase (OASS) inhibitor can be used as a colistin adjuvant to treat infections caused by...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00378

    authors： Annunziato G,Spadini C,Franko N,Storici P,Demitri N,Pieroni M,Flisi S,Rosati L,Iannarelli M,Marchetti M,Magalhaes J,Bettati S,Mozzarelli A,Cabassi CS,Campanini B,Costantino G

    更新日期：2021-01-29 00:00:00

  • Rapid in Vitro Assessment of Clostridioides difficile Inhibition by Probiotics Using Dielectrophoresis to Quantify Cell Structure Alterations.

    abstract：:Clostridioides difficile (C. difficile) infection (CDI) is the primary cause of nosocomial antibiotic-associated diarrhea, with high recurrence rates following initial antibiotic treatment regimens. Restoration of the host gut microbiome through probiotic therapy is under investigation to reduce recurrence. Current in...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00415

    authors： Moore JH,Honrado C,Stagnaro V,Kolling G,Warren CA,Swami NS

    更新日期：2020-05-08 00:00:00

  • A Methyl 4-Oxo-4-phenylbut-2-enoate with in Vivo Activity against MRSA that Inhibits MenB in the Bacterial Menaquinone Biosynthesis Pathway.

    abstract：:4-Oxo-4-phenyl-but-2-enoates inhibit MenB, the 1,4-dihydroxyl-2-naphthoyl-CoA synthase in the bacterial menaquinone (MK) biosynthesis pathway, through the formation of an adduct with coenzyme A (CoA). Here, we show that the corresponding methyl butenoates have MIC values as low as 0.35-0.75 µg/mL against drug sensitiv...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.6b00023

    authors： Matarlo JS,Lu Y,Daryaee F,Daryaee T,Ruzsicska B,Walker SG,Tonge PJ

    更新日期：2016-05-13 00:00:00

  • Preclinical Antiviral Testing for Dengue Virus Infection in Mouse Models and Its Association with Clinical Studies.

    abstract：:At present, there is no licensed antiviral drug against dengue virus (DENV) infection. Mouse models of DENV infection have been widely used for preclinical evaluation of antivirals. However, only in a few instances so far have the data obtained from preclinical mouse model testing been associated with data from clinic...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.8b00054

    authors： Watanabe S,Low JG,Vasudevan SG

    更新日期：2018-07-13 00:00:00

  • High-Throughput Minigenome System for Identifying Small-Molecule Inhibitors of Ebola Virus Replication.

    abstract：:Ebola virus (EBOV), a member of the family Filoviridae, is a nonsegmented negative-sense RNA virus that causes severe, often lethal, disease in humans. EBOV RNA synthesis is carried out by a complex that includes several viral proteins. The function of this machinery is essential for viral gene expression and viral re...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.5b00053

    authors： Edwards MR,Pietzsch C,Vausselin T,Shaw ML,Bukreyev A,Basler CF

    更新日期：2015-08-14 00:00:00

  • Molecular Mechanism of Avibactam-Mediated β-Lactamase Inhibition.

    abstract：:Emerging β-lactamase-mediated resistance is threatening the clinical utility of the single most prominent class of antibacterial agents used in medicine, the β-lactams. The diazabicyclooctane avibactam is able to inhibit a wider range of serine β-lactamases than has been previously observed with β-lactamase inhibitors...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.5b00007

    authors： King DT,King AM,Lal SM,Wright GD,Strynadka NC

    更新日期：2015-04-10 00:00:00

  • Host-Mediated Bioactivation of Pyrazinamide: Implications for Efficacy, Resistance, and Therapeutic Alternatives.

    abstract：:Pyrazinamide has played a critical role in shortening therapy against drug-sensitive, drug-resistant, active, and latent tuberculosis (TB). Despite widespread recognition of its therapeutic importance, the sterilizing properties of this 60-year-old drug remain an enigma given its rather poor activity in vitro. Here we...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/id500028m

    authors： Via LE,Savic R,Weiner DM,Zimmerman MD,Prideaux B,Irwin SM,Lyon E,O'Brien P,Gopal P,Eum S,Lee M,Lanoix JP,Dutta NK,Shim T,Cho JS,Kim W,Karakousis PC,Lenaerts A,Nuermberger E,Barry CE 3rd,Dartois V

    更新日期：2015-05-08 00:00:00

  • Mechanistic Evaluation of Lipopolysaccharide-Alexidine Interaction Using Spectroscopic and in Silico Approaches.

    abstract：:The increasing problem of multidrug resistance (MDR) in bacteria calls for discovery of new molecules and diagnostic methodologies that are effective against a wide range of microbial pathogens. We have studied the role of alexidine dihydrochloride (alex) as a bioaffinity ligand against lipopolysaccharide (LPS), a pat...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.8b00087

    authors： Jagtap P,Mishra R,Khanna S,Kumari P,Mittal B,Kashyap HK,Gupta S

    更新日期：2018-11-09 00:00:00

  • Staphylococcus aureus Specific FRET Probe-Based Antibacterial Susceptibility Testing (SF-AST) by Detection of Micrococcal Nuclease Activity.

    abstract：:In this study, we describe a simple and rapid antibacterial susceptibility testing (AST) method for Staphylococcus aureus called S. aureus specific fluorescence resonance energy transfer (FRET) probe-based AST (SF-AST), which is based on an S. aureus specific FRET probe (SF probe) that detects micrococcal nuclease (MN...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00260

    authors： Yi SY,Jeong J,Kim KE,Park K,Shin YB

    更新日期：2020-02-14 00:00:00

  • High-Throughput Immunochemical Method to Assess the 2-Heptyl-4-quinolone Quorum Sensing Molecule as a Potential Biomarker of Pseudomonas aeruginosa Infections.

    abstract：:Bacterial quorum sensing (QS) is being contemplated as a promising target for developing innovative diagnostic and therapeutic strategies. Here we report for the first time the development of antibodies against 2-heptyl-4-quinolone (HHQ), a signaling molecule from the pqs QS system of Pseudomonas aeruginosa, involved ...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00604

    authors： Montagut EJ,Vilaplana L,Martin-Gomez MT,Marco MP

    更新日期：2020-12-11 00:00:00

  • Violacein Targets the Cytoplasmic Membrane of Bacteria.

    abstract：:Violacein is a tryptophan-derived purple pigment produced by environmental bacteria, which displays multiple biological activities, including strong inhibition of Gram-positive pathogens. Here, we applied a combination of experimental approaches to identify the mechanism by which violacein kills Gram-positive bacteria...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.8b00245

    authors： Cauz ACG,Carretero GPB,Saraiva GKV,Park P,Mortara L,Cuccovia IM,Brocchi M,Gueiros-Filho FJ

    更新日期：2019-04-12 00:00:00

  • Diaza-anthracene Antibiotics from a Freshwater-Derived Actinomycete with Selective Antibacterial Activity toward Mycobacterium tuberculosis.

    abstract：:Multidrug- and extensively drug-resistant strains of Mycobacterium tuberculosis are resistant to first- and second-line drug regimens and resulted in 210,000 fatalities in 2013. In the current study, we screened a library of aquatic bacterial natural product fractions for their ability to inhibit this pathogen. A frac...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.5b00005

    authors： Mullowney MW,Hwang CH,Newsome AG,Wei X,Tanouye U,Wan B,Carlson S,Barranis NJ,hAinmhire E,Chen WL,Krishnamoorthy K,White J,Blair R,Lee H,Burdette JE,Rathod PK,Parish T,Cho S,Franzblau SG,Murphy BT

    更新日期：2015-04-10 00:00:00

  • Chemical Warfare at the Microorganismal Level: A Closer Look at the Superoxide Dismutase Enzymes of Pathogens.

    abstract：:Superoxide anion radical is generated as a natural byproduct of aerobic metabolism but is also produced as part of the oxidative burst of the innate immune response design to kill pathogens. In living systems, superoxide is largely managed through superoxide dismutases (SODs), families of metalloenzymes that use Fe, M...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.8b00026

    authors： Schatzman SS,Culotta VC

    更新日期：2018-06-08 00:00:00

  • Systematic Analysis of Structure Similarity between Zika Virus and Other Flaviviruses.

    abstract：:Zika virus (ZIKV) infection has caused global concern because of its association with fetal microcephaly and serious neurological complications in adults since 2016. Currently, no specific anti-ZIKV therapy is available to control ZIKV infection. During the last couple of years, the intensive investigation of ZIKV str...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.9b00047

    authors： Hu Y,Sun L

    更新日期：2019-07-12 00:00:00

  • 6-Hydroxydopamine Inhibits the Hepatitis C Virus through Alkylation of Host and Viral Proteins and the Induction of Oxidative Stress.

    abstract：:Many viruses, including the hepatitis C virus (HCV), are dependent on the host RNA silencing pathway for replication. In this study, we screened small molecule probes, previously reported to disrupt loading of the RNA-induced silencing complex (RISC), including 6-hydroxydopamine (6-OHDA), suramin (SUR), and aurintrica...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.6b00098

    authors： Lafreniere MA,Powdrill MH,Singaravelu R,Pezacki JP

    更新日期：2016-11-11 00:00:00

  • Repositioning of Guanabenz in Conjugation with Gold and Silver Nanoparticles against Pathogenic Amoebae Acanthamoeba castellanii and Naegleria fowleri.

    abstract：:Brain-eating amoebae cause devastating infections in the central nervous system of humans, resulting in a mortality rate of 95%. There are limited effective therapeutic options available clinically for treating granulomatous amoebic encephalitis and primary amoebic meningoencephalitis caused by Acanthamoeba castellani...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00263

    authors： Anwar A,Mungroo MR,Anwar A,Sullivan WJ Jr,Khan NA,Siddiqui R

    更新日期：2019-12-13 00:00:00

  • Antagonizing Vancomycin Resistance in Enterococcus by Surface Localized Antimicrobial Display-Derived Peptides.

    abstract：:Decreasing the therapeutic pipeline for vancomycin-resistant Enterococci (VRE) calls for novel strategies to enhance our antibacterial arsenal. Herein, we investigated the potential applications of surface localized antimicrobial display (SLAY)-derived cationic peptides in the fight against VanA operon mediated vancom...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00164

    authors： Liu Y,Jia Y,Yang K,Li R,Xiao X,Wang Z

    更新日期：2020-05-08 00:00:00

  • Discrimination of Escherichia coli and Shigella spp. by Nuclear Magnetic Resonance Based Metabolomic Characterization of Culture Media.

    abstract：:Dysentery is a major health threat that dramatically impacts childhood morbidity and mortality in developing countries. Various pathogenic agents cause dysentery, such as Shigella spp. and Escherichia coli, which are very closely related if not identical species. Sensitive and precise detection and identification of t...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00199

    authors： Rautureau GJP,Palama TL,Canard I,Mirande C,Chatellier S,van Belkum A,Elena-Herrmann B

    更新日期：2019-11-08 00:00:00

  • Identification of Trypanosoma brucei AdoMetDC Inhibitors Using a High-Throughput Mass Spectrometry-Based Assay.

    abstract：:Human African trypanosomiasis (HAT) is a fatal infectious disease caused by the eukaryotic pathogen Trypanosoma brucei (Tb). Available treatments are difficult to administer and have significant safety issues. S-Adenosylmethionine decarboxylase (AdoMetDC) is an essential enzyme in the parasite polyamine biosynthetic p...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.7b00022

    authors： Volkov OA,Cosner CC,Brockway AJ,Kramer M,Booker M,Zhong S,Ketcherside A,Wei S,Longgood J,McCoy M,Richardson TE,Wring SA,Peel M,Klinger JD,Posner BA,De Brabander JK,Phillips MA

    更新日期：2017-07-14 00:00:00

  • Biogenic and Biomimetic Carriers as Versatile Transporters To Treat Infections.

    abstract：:Biogenic and biomimetic therapeutics are a relatively new class of systems that are of physiological origin and/or take advantage of natural pathways or aim at mimicking these to improve selective interaction with target tissue. The number of biogenic and bioengineered avenues for drug therapy and diagnostics has mult...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.8b00030

    authors： Goes A,Fuhrmann G

    更新日期：2018-06-08 00:00:00

  • Fitness Landscape of the Immune Compromised Favors the Emergence of Antibiotic Resistance.

    abstract：:Antibiotic resistance can come at a high cost, both in terms of fitness for the pathogen and poorer outcomes for patients. The fitness landscape encountered by bacterial pathogens varies greatly throughout patient populations in terms of host immunity as well as the duration and spectrum of antibiotics encountered. Se...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.8b00158

    authors： Margolis E,Rosch JW

    更新日期：2018-09-14 00:00:00