当前位置： SCI文献检索 > ACS Infectious Diseases期刊下所有文献 > Rapid Isolation, Propagation, and Online Analysis of a Small Number of Therapeutic Staphylococcal Bacteriophages from a Complex Matrix.

Rapid Isolation, Propagation, and Online Analysis of a Small Number of Therapeutic Staphylococcal Bacteriophages from a Complex Matrix.

Abstract：

:A method for the fast isolation, propagation, and characterization of very low count bacteriophages active against pathogenic bacterial strains is described in this study. Bacteriophages with a count of 102 phage particles were dynamically adhered from the maximum 10 mL blood plasma sample onto the nanostructured part of the fused silica capillary. One-step propagation of phage particles of genus Kayvirus inside the etched capillary on 104Staphylococcus aureus host cells increased their number to 6 × 104 phage particles. Phage particles were concentrated online and separated by capillary electrophoretic methods. No phage replication occurred when the phage-resistant S. aureus or Escherichia coli cells were used. Two-step phage propagation in the capillary allowed an increase in the total virion count to up to 6 × 105 phage particles and subsequent off-line matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of the phage zone collected after capillary electrophoresis. Relative standard deviations of the phage peak area were at most 2.3%. We expect that the method of isolating bacteriophages from blood plasma and their simultaneous identification will facilitate clinical studies of phage preparations and contribute to pharmacokinetics studies during phage therapy. This approach is also suitable for capturing and enriching new phages from the environment when a susceptible indicator strain is available.

journal_name

ACS Infect Dis

journal_title

ACS infectious diseases

authors

Horká M,Šalplachta J,Karásek P,Růžička F,Štveráková D,Pantůček R,Roth M

doi

10.1021/acsinfecdis.0c00358

subject

Has Abstract

pub_date

2020-10-09 00:00:00

pages

2745-2755

issue

10

issn

2373-8227

journal_volume

6

pub_type

杂志文章

相关文献

ACS Infectious Diseases文献大全
  • Antibodies That Engage the Hemagglutinin Receptor-Binding Site of Influenza B Viruses.

    abstract：:We describe cross-reactive human antibodies recognizing influenza B viruses spanning nearly 80 years of antigenic drift. Structures show that they engage the receptor-binding site (RBS) of the viral hemagglutinin with strong similarities to their influenza A counterparts, despite structural differences between the RBS...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00726

    authors： Bajic G,Harrison SC

    更新日期：2021-01-08 00:00:00

  • Virus-like Particle Display of the α-Gal Epitope for the Diagnostic Assessment of Chagas Disease.

    abstract：:The α-Gal antigen [Galα(1,3)Galβ(1,4)GlcNAcα] is an immunodominant epitope displayed by infective trypomastigote forms of Trypanosoma cruzi, the causative agent of Chagas disease. A virus-like particle displaying a high density of α-Gal was found to be a superior reagent for the ELISA-based serological diagnosis of Ch...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.6b00114

    authors： Brito CR,McKay CS,Azevedo MA,Santos LC,Venuto AP,Nunes DF,D'Ávila DA,Rodrigues da Cunha GM,Almeida IC,Gazzinelli RT,Galvão LM,Chiari E,Sanhueza CA,Finn MG,Marques AF

    更新日期：2016-12-09 00:00:00

  • Chemical Control of Quorum Sensing in E. coli: Identification of Small Molecule Modulators of SdiA and Mechanistic Characterization of a Covalent Inhibitor.

    abstract：:Enterohemorrhagic Escherichia coli (EHEC) is the causative agent of severe diarrheal disease in humans. Cattle are the natural reservoir of EHEC, and approximately 75% of EHEC infections in humans stem from bovine products. Many common bacterial pathogens, including EHEC, rely on chemical communication systems, such a...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00654

    authors： Styles MJ,Early SA,Tucholski T,West KHJ,Ge Y,Blackwell HE

    更新日期：2020-12-11 00:00:00

  • Introduction of a Thio Functional Group to Diazabicyclooctane: An Effective Modification to Potentiate the Activity of β-Lactams against Gram-Negative Bacteria Producing Class A, C, and D Serine β-Lactamases.

    abstract：:By the emergence and worldwide spread of multi-drug-resistant Gram-negative bacteria, there have been growing demands for efficacious drugs to cure these resistant infections. The key mechanism for resistance to β-lactam antibiotics is the production of β-lactamases, which hydrolyze and deactivate β-lactams. Diazabicy...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00560

    authors： Fujiu M,Yokoo K,Sato J,Shibuya S,Komano K,Kusano H,Sato S,Aoki T,Kohira N,Miyagawa S,Kawachi T,Yamawaki K

    更新日期：2020-11-13 00:00:00

  • Effect of Composition on Antibacterial Activity of Sequence-Defined Cationic Oligothioetheramides.

    abstract：:In response to the urgent need for new antibiotic development strategies, antimicrobial peptides and their synthetic mimetics are being investigated as promising alternatives to traditional antibiotics. To facilitate their development into clinically viable candidates, we need to understand what molecular features and...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.8b00079

    authors： Artim CM,Phan NN,Alabi CA

    更新日期：2018-08-10 00:00:00

  • Substrate Tolerance of Bacterial Glycosyltransferase MurG: Novel Fluorescence-Based Assays.

    abstract：:MurG (uridine diphosphate-N-acetylglucosamine/N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase) is an essential bacterial glycosyltransferase that catalyzes the N-acetylglucosamine (GlcNAc) transformation of lipid I to lipid II during peptidoglycan biosynthesis. Park's nucleot...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00242

    authors： Mitachi K,Yun HG,Gillman CD,Skorupinska-Tudek K,Swiezewska E,Clemons WM Jr,Kurosu M

    更新日期：2020-06-12 00:00:00

  • A Methyl 4-Oxo-4-phenylbut-2-enoate with in Vivo Activity against MRSA that Inhibits MenB in the Bacterial Menaquinone Biosynthesis Pathway.

    abstract：:4-Oxo-4-phenyl-but-2-enoates inhibit MenB, the 1,4-dihydroxyl-2-naphthoyl-CoA synthase in the bacterial menaquinone (MK) biosynthesis pathway, through the formation of an adduct with coenzyme A (CoA). Here, we show that the corresponding methyl butenoates have MIC values as low as 0.35-0.75 µg/mL against drug sensitiv...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.6b00023

    authors： Matarlo JS,Lu Y,Daryaee F,Daryaee T,Ruzsicska B,Walker SG,Tonge PJ

    更新日期：2016-05-13 00:00:00

  • Targeting Polyamines Inhibits Coronavirus Infection by Reducing Cellular Attachment and Entry.

    abstract：:Coronaviruses first garnered widespread attention in 2002 when the severe acute respiratory syndrome coronavirus (SARS-CoV) emerged from bats in China and rapidly spread in human populations. Since then, Middle East respiratory syndrome coronavirus (MERS-CoV) emerged and still actively infects humans. The recent SARS-...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00491

    authors： Firpo MR,Mastrodomenico V,Hawkins GM,Prot M,Levillayer L,Gallagher T,Simon-Loriere E,Mounce BC

    更新日期：2020-10-06 00:00:00

  • Antagonizing Vancomycin Resistance in Enterococcus by Surface Localized Antimicrobial Display-Derived Peptides.

    abstract：:Decreasing the therapeutic pipeline for vancomycin-resistant Enterococci (VRE) calls for novel strategies to enhance our antibacterial arsenal. Herein, we investigated the potential applications of surface localized antimicrobial display (SLAY)-derived cationic peptides in the fight against VanA operon mediated vancom...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00164

    authors： Liu Y,Jia Y,Yang K,Li R,Xiao X,Wang Z

    更新日期：2020-05-08 00:00:00

  • 6-Hydroxydopamine Inhibits the Hepatitis C Virus through Alkylation of Host and Viral Proteins and the Induction of Oxidative Stress.

    abstract：:Many viruses, including the hepatitis C virus (HCV), are dependent on the host RNA silencing pathway for replication. In this study, we screened small molecule probes, previously reported to disrupt loading of the RNA-induced silencing complex (RISC), including 6-hydroxydopamine (6-OHDA), suramin (SUR), and aurintrica...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.6b00098

    authors： Lafreniere MA,Powdrill MH,Singaravelu R,Pezacki JP

    更新日期：2016-11-11 00:00:00

  • Signed, Sealed, Delivered: Conjugate and Prodrug Strategies as Targeted Delivery Vectors for Antibiotics.

    abstract：:Innate and developed resistance mechanisms of bacteria to antibiotics are obstacles in the design of novel drugs. However, antibacterial prodrugs and conjugates have shown promise in circumventing resistance and tolerance mechanisms via directed delivery of antibiotics to the site of infection or to specific species o...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.9b00019

    authors： Cheng AV,Wuest WM

    更新日期：2019-06-14 00:00:00

  • High-Throughput Immunochemical Method to Assess the 2-Heptyl-4-quinolone Quorum Sensing Molecule as a Potential Biomarker of Pseudomonas aeruginosa Infections.

    abstract：:Bacterial quorum sensing (QS) is being contemplated as a promising target for developing innovative diagnostic and therapeutic strategies. Here we report for the first time the development of antibodies against 2-heptyl-4-quinolone (HHQ), a signaling molecule from the pqs QS system of Pseudomonas aeruginosa, involved ...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00604

    authors： Montagut EJ,Vilaplana L,Martin-Gomez MT,Marco MP

    更新日期：2020-12-11 00:00:00

  • Detection of Bacterial Coinfection in COVID-19 Patients Is a Missing Piece of the Puzzle in the COVID-19 Management in Indonesia.

    abstract：:Bacterial coinfection in COVID-19 patients has the potential to complicate treatments and accelerate the development of antibiotic resistance in the clinic due to the widespread use of broad-spectrum antibiotics, including in Indonesia. The surge of COVID-19 patients may worsen antibiotic overuse; therefore, informati...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.1c00006

    authors： Prasetyoputri A

    更新日期：2021-01-27 00:00:00

  • Chemical Warfare at the Microorganismal Level: A Closer Look at the Superoxide Dismutase Enzymes of Pathogens.

    abstract：:Superoxide anion radical is generated as a natural byproduct of aerobic metabolism but is also produced as part of the oxidative burst of the innate immune response design to kill pathogens. In living systems, superoxide is largely managed through superoxide dismutases (SODs), families of metalloenzymes that use Fe, M...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.8b00026

    authors： Schatzman SS,Culotta VC

    更新日期：2018-06-08 00:00:00

  • Instructive Advances in Chemical Microbiology Inspired by Nature's Diverse Inventory of Molecules.

    abstract：:Natural product antibiotics have played an essential role in the treatment of bacterial infection in addition to serving as useful tools to explore the intricate biology of bacteria. Our current arsenal of antibiotics operate through the inhibition of well-defined bacterial targets critical for replication and growth....

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.9b00413

    authors： Liu K,Huigens RW 3rd

    更新日期：2020-04-10 00:00:00

  • Identification of Trypanosoma brucei AdoMetDC Inhibitors Using a High-Throughput Mass Spectrometry-Based Assay.

    abstract：:Human African trypanosomiasis (HAT) is a fatal infectious disease caused by the eukaryotic pathogen Trypanosoma brucei (Tb). Available treatments are difficult to administer and have significant safety issues. S-Adenosylmethionine decarboxylase (AdoMetDC) is an essential enzyme in the parasite polyamine biosynthetic p...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.7b00022

    authors： Volkov OA,Cosner CC,Brockway AJ,Kramer M,Booker M,Zhong S,Ketcherside A,Wei S,Longgood J,McCoy M,Richardson TE,Wring SA,Peel M,Klinger JD,Posner BA,De Brabander JK,Phillips MA

    更新日期：2017-07-14 00:00:00

  • N-Myristoylation as a Drug Target in Malaria: Exploring the Role of N-Myristoyltransferase Substrates in the Inhibitor Mode of Action.

    abstract：:Malaria continues to be a significant cause of death and morbidity worldwide, and there is a need for new antimalarial drugs with novel targets. We have focused as a potential target for drug development on N-myristoyl transferase (NMT), an enzyme that acylates a wide range of substrate proteins. The NMT substrates in...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.7b00203

    authors： Schlott AC,Holder AA,Tate EW

    更新日期：2018-04-13 00:00:00

  • Daptomycin Pore Formation Is Restricted by Lipid Acyl Chain Composition.

    abstract：:Daptomycin is a calcium-dependent lipopeptide antibiotic that is used clinically against various Gram-positive pathogens. It acts on bacterial cell membranes, whose susceptibility varies with the content of phosphatidylglycerol (PG). Some studies have reported that daptomycin permeabilizes and depolarizes bacterial ce...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.7b00138

    authors： Taylor R,Beriashvili D,Taylor S,Palmer M

    更新日期：2017-11-10 00:00:00

  • Combining 25-Hydroxycholesterol with an HIV Fusion Inhibitor Peptide: Interaction with Biomembrane Model Systems and Human Blood Cells.

    abstract：:The fusion between the viral and the target cell membrane is a crucial step in the life cycle of enveloped viruses. The blocking of this process is a well-known therapeutic approach that led to the development of the fusion inhibitor peptide enfuvirtide, clinically used against human immunodeficiency virus (HIV) type ...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.8b00321

    authors： Gomes B,Sanna G,Madeddu S,Hollmann A,Santos NC

    更新日期：2019-04-12 00:00:00

  • REP 2139: Antiviral Mechanisms and Applications in Achieving Functional Control of HBV and HDV Infection.

    abstract：:Nucleic acid polymers (NAPs) are broad spectrum antiviral agents whose antiviral activity in hepatitis B virus (HBV) infection is derived from their ability to block the release of the hepatitis B virus surface antigen (HBsAg). This pharmacological activity blocks replenishment of HBsAg in the circulation, allowing ho...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.8b00156

    authors： Vaillant A

    更新日期：2019-05-10 00:00:00

  • Clinical and Laboratory Diagnosis of SARS-CoV-2, the Virus Causing COVID-19.

    abstract：:In December 2019, a novel beta (β) coronavirus eventually named SARS-CoV-2 emerged in Wuhan, Hubei province, China, causing an outbreak of severe and even fatal pneumonia in humans. The virus has spread very rapidly to many countries across the world, resulting in the World Health Organization (WHO) to declare a pande...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.0c00274

    authors： da Silva SJR,Silva CTAD,Guarines KM,Mendes RPG,Pardee K,Kohl A,Pena L

    更新日期：2020-09-11 00:00:00

  • COVID-19 and Other Pandemics: How Might They Be Prevented?

    abstract：:Pandemics such as influenza, smallpox, and plague have caused the loss of hundreds of millions of lives and have occurred for many centuries. Fortunately, they have been largely eliminated by the use of vaccinations and drugs. More recently, Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (M...

    journal_title：ACS infectious diseases

    pub_type： 历史文章,杂志文章,评审

    doi：10.1021/acsinfecdis.0c00291

    authors： Oldfield E,Malwal SR

    更新日期：2020-07-10 00:00:00

  • 1,2,3-Triazolylmethaneboronate: A Structure Activity Relationship Study of a Class of β-Lactamase Inhibitors against Acinetobacter baumannii Cephalosporinase.

    abstract：:Boronic acid transition state inhibitors (BATSIs) are known reversible covalent inhibitors of serine β-lactamases. The selectivity and high potency of specific BATSIs bearing an amide side chain mimicking the β-lactam's amide side chain are an established and recognized synthetic strategy. Herein, we describe a new cl...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00254

    authors： Caselli E,Fini F,Introvigne ML,Stucchi M,Taracila MA,Fish ER,Smolen KA,Rather PN,Powers RA,Wallar BJ,Bonomo RA,Prati F

    更新日期：2020-07-10 00:00:00

  • Inhibiting Iron Mobilization from Bacterioferritin in Pseudomonas aeruginosa Impairs Biofilm Formation Irrespective of Environmental Iron Availability.

    abstract：:Although iron is essential for bacteria, the nutrient presents problems of toxicity and solubility. Bacteria circumvent these problems with the aid of iron storage proteins where Fe3+ is deposited and, when necessary, mobilized as Fe2+ for metabolic requirements. In Pseudomonas aeruginosa, Fe3+ is compartmentalized in...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.9b00398

    authors： Soldano A,Yao H,Chandler JR,Rivera M

    更新日期：2020-03-13 00:00:00

  • Toward Curative Immunomodulation Strategies for Chronic Hepatitis B Virus Infection.

    abstract：:Chronic hepatitis B virus (HBV) infection remains a major cause of morbidity and mortality worldwide. HBV surface antigen loss is considered a functional cure and is an ideal goal for antiviral therapy. However, current treatment regimens, including nucleos(t)ide analogues or interferons monotherapy and combination th...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章,评审

    doi：10.1021/acsinfecdis.8b00297

    authors： Zhu W,Liu H,Zhang X

    更新日期：2019-05-10 00:00:00

  • Development of Antibiotics That Dysregulate the Neisserial ClpP Protease.

    abstract：:Evolving antimicrobial resistance has motivated the search for novel targets and alternative therapies. Caseinolytic protease (ClpP) has emerged as an enticing new target since its function is conserved and essential for bacterial fitness, and because its inhibition or dysregulation leads to bacterial cell death. ClpP...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00599

    authors： Binepal G,Mabanglo MF,Goodreid JD,Leung E,Barghash MM,Wong KS,Lin F,Cossette M,Bansagi J,Song B,Balasco Serrão VH,Pai EF,Batey RA,Gray-Owen SD,Houry WA

    更新日期：2020-12-11 00:00:00

  • Permeability Barrier of Gram-Negative Cell Envelopes and Approaches To Bypass It.

    abstract：:Gram-negative bacteria are intrinsically resistant to many antibiotics. Species that have acquired multidrug resistance and cause infections that are effectively untreatable present a serious threat to public health. The problem is broadly recognized and tackled at both the fundamental and applied levels. This paper s...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.5b00097

    authors： Zgurskaya HI,Löpez CA,Gnanakaran S

    更新日期：2015-01-01 00:00:00

  • Synergistic Effect of Combined Artesunate and Tetramethylpyrazine in Experimental Cerebral Malaria.

    abstract：:Intravenous artesunate is effective against cerebral malaria (CM), but high mortality and neurological sequelae in survivors are inevitable. We investigated the effect of combined artesunate and tetramethylpyrazine using mouse models of experimental cerebral malaria (ECM). Artesunate + tetramethylpyrazine reduced micr...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00124

    authors： Jiang X,Chen L,Zheng Z,Chen Y,Weng X,Guo Y,Li K,Yang T,Qu S,Liu H,Li Y,Zhu X

    更新日期：2020-09-11 00:00:00

  • A Mitochondrial Orthologue of the dNTP Triphosphohydrolase SAMHD1 Is Essential and Controls Pyrimidine Homeostasis in Trypanosoma brucei.

    abstract：:The maintenance of deoxyribonucleotide triphosphate (dNTP) homeostasis through synthesis and degradation is critical for accurate genomic and mitochondrial DNA replication fidelity. Trypanosoma brucei makes use of both the salvage and de novo pathways for the provision of pyrimidine dNTPs. In this respect, the sterile...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00551

    authors： Yagüe-Capilla M,Castillo-Acosta VM,Bosch-Navarrete C,Ruiz-Pérez LM,González-Pacanowska D

    更新日期：2021-01-08 00:00:00

  • Two-Way Regulation of MmpL3 Expression Identifies and Validates Inhibitors of MmpL3 Function in Mycobacterium tuberculosis.

    abstract：:MmpL3, an essential mycolate transporter in the inner membrane of Mycobacterium tuberculosis (Mtb), has been identified as a target of multiple, chemically diverse antitubercular drugs. However, several of these molecules seem to have secondary targets and inhibit bacterial growth by more than one mechanism. Here, we ...

    journal_title：ACS infectious diseases

    pub_type： 杂志文章

    doi：10.1021/acsinfecdis.0c00675

    authors： Grover S,Engelhart CA,Pérez-Herrán E,Li W,Abrahams KA,Papavinasasundaram K,Bean JM,Sassetti CM,Mendoza-Losana A,Besra GS,Jackson M,Schnappinger D

    更新日期：2021-01-08 00:00:00