Expression, purification and characterization of Der f 27, a new allergen from dermatophagoides farinae.

Abstract:

:The house dust mite (HDM), Dermatophagoidesfarinae (D. farina), is one of the most important indoor allergen sources and a major elicitor of allergic asthma; itscharacterization is important in the diagnosis and immunotherapy of mite allergen-relevant diseases. This study aims to characterize a novel allergen, the D. farinae-derived serpin (Der f 27). In this study, the total RNA of D. farinae was extracted, and the Der f 27 gene was cloned and expressed. The allergenicity of recombinant Der f 27 protein was determined by enzyme-linked immunosorbent assay, and Western-blotting with the sera of asthma patients, and skin prick test (SPT) in allergic human subjects. A r-Der f 27 allergic asthma mouse model was established. The cloned Der f 27 gene has been presented at the Gene Bank with an accession number of KM009995. The IgE levels of r-Der f 27 in the serum from r-Der f 27 SPT positive allergic patients were 3 folds more than healthy subjects. The Der f 27 SPT positive ratewas 42.1% in 19 DM-SPT positive patients. Airway hyperresponsiveness, serum specific IgE, and levels of interleukin-4 in the spleen cell culture supernatant were significantly increased in allergic asthma mice sensitized to r-Der f 27. In conclusion, Der f 27 is a new subtype of house mite allergen.

journal_name

Am J Transl Res

authors

Lin J,Li M,Liu Y,Jiang C,Wu Y,Wang Y,Gao A,Liu Z,Yang P,Liu X

subject

Has Abstract

pub_date

2015-07-15 00:00:00

pages

1260-70

issue

7

issn

1943-8141

journal_volume

7

pub_type

杂志文章
  • Adhesion: a confounding bias in murine cervical heterotopic heart transplantation.

    abstract::Tissue adhesion is a common postsurgical phenomenon among the human population. This complication also occurs in murine transplant models. In this study, we investigated the impact of adhesion on murine cervical heterotopic heart transplantation by using sodium hyaluronate (SH) as an anti-adhesive agent. Our study rev...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Yang J,Chen Q,Liu F,Fu Z,Wang Q

    更新日期:2015-09-15 00:00:00

  • Exercise training and immune crosstalk in breast cancer microenvironment: exploring the paradigms of exercise-induced immune modulation and exercise-induced myokines.

    abstract::Observational research suggests that exercise may reduce the risk of breast cancer and improve survival. One proposed mechanism for the protective effect of aerobic exercise related to cancer risk and outcomes, but has not been examined definitively, is the immune response to aerobic exercise. Two prevailing paradigms...

    journal_title:American journal of translational research

    pub_type: 杂志文章,评审

    doi:

    authors: Goh J,Niksirat N,Campbell KL

    更新日期:2014-10-11 00:00:00

  • Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and noncanonical caspase-11-mediated pyroptosis pathways.

    abstract::This study aimed to verify the effects of calpain on coxsackievirus B3 (CVB3)-induced myocarditis and to further explore the underlying mechanisms. Transgenic mice overexpressing calpastatin, the endogenous calpain inhibitor, were introduced in the present study. The murine model of viral myocarditis (VMC) was establi...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Yu Y,Shi H,Yu Y,Liu M,Li M,Liu X,Wang Y,Chen R

    更新日期:2020-05-15 00:00:00

  • LncRNA MEG3-derived miR-361-5p regulate vascular smooth muscle cells proliferation and apoptosis by targeting ABCA1.

    abstract:BACKGROUND:Atherosclerosis remains a leading cause of cardiology disease worldwide, which vascular smooth muscle cells (VSMCs) proliferation and apoptosis are involved. Increasing evidences have revealed that long non-coding RNAs (lncRNAs) considered to be critical regulatory factors of VSMCs function. However, the mol...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wang M,Li C,Zhang Y,Zhou X,Liu Y,Lu C

    更新日期:2019-06-15 00:00:00

  • Tomographic analysis of the temporomandibular joint in patients with arthritis: a case of disease translation in Yucatan, Mexico.

    abstract::Computed tomography imaging of the temporomandibular joint was carried out in 22 previously-diagnosed arthritis patients (3 men, 19 women). This descriptive, cross-sectional observational, qualitative study allowed to characterize the type of condylar morphology condition, the space between temporomandibular joint, th...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Mendiburu-Zavala C,Camargo-Riqué ME,Peñaloza-Cuevas R,Cárdenas-Erosa R,Lara-Flores M,Castaño VM

    更新日期:2019-01-15 00:00:00

  • CircRNAs: potency of protein translation and feasibility of novel biomarkers and therapeutic targets for head and neck cancers.

    abstract::Circular RNAs (circRNAs), a new star noncoding RNA (ncRNA), show stability, conservation, abundance, and tissue and stage specificity. They act as key regulators of biological processes. They target the mRNAs of many other different genes or signaling pathways, and closely link associated genes into regulatory network...

    journal_title:American journal of translational research

    pub_type: 杂志文章,评审

    doi:

    authors: Zhou DN,Ye CS,Deng YF

    更新日期:2020-05-15 00:00:00

  • Endothelial MMP-9 drives the inflammatory response in abdominal aortic aneurysm (AAA).

    abstract::Progression of abdominal aortic aneurysm (AAA) is typified by chronic inflammation and extracellular matrix (ECM) degradation of the aortic wall. Vascular inflammation involves complex interactions among inflammatory cells, endothelial cells (ECs), vascular smooth muscle cells (vSMCs), and ECM. Although vascular endot...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Ramella M,Boccafoschi F,Bellofatto K,Follenzi A,Fusaro L,Boldorini R,Casella F,Porta C,Settembrini P,Cannas M

    更新日期:2017-12-15 00:00:00

  • Identification of mesothelial cells in intraoperative blood salvage.

    abstract::Intraoperative auto-transfusion with the use of cell saver systems is routinely used to reduce the rate of packed red blood transfusion in major surgery. Nevertheless some concerns have been raised on possible risks of coagulation disorders. The aim of the study was to analyze the blood processed by the cell saver, re...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Santise G,Maselli D,Malanga D,Di Vito A,Mandarino N,Boccadamo G,Zeppa P,Amorosi A,Viglietto G,Rizzuto A,Mignogna C

    更新日期:2019-03-15 00:00:00

  • Combination therapy with extracorporeal shock wave and melatonin markedly attenuated neuropathic pain in rat.

    abstract::This study tested the hypothesis that combination therapy using extracorporeal shock wave (ECSW)-melatonin (Mel) was superior to either alone at ameliorating neuropathic pain (NP). NP was induced by chronic constriction injury (CCI) to the left sciatic nerve in rats. Animals were categorized into sham control (group 1...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Chen KH,Yang CH,Wallace CG,Lin CR,Liu CK,Yin TC,Huang TH,Chen YL,Sun CK,Yip HK

    更新日期:2017-10-15 00:00:00

  • Akt blocks the tumor suppressor activity of LKB1 by promoting phosphorylation-dependent nuclear retention through 14-3-3 proteins.

    abstract::The survival kinase Akt and the tumor suppressor LKB1 elicit opposite effects on cell proliferation and tumorigenesis. The present study demonstrates that Akt acts as an upstream kinase of LKB1 to promote the phosphorylation at Ser334 and facilitate its binding to 14-3-3 proteins, resulting in a decreased interaction ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Liu L,Siu FM,Che CM,Xu A,Wang Y

    更新日期:2012-01-01 00:00:00

  • Inhibition of N-acetyltransferase 10 using remodelin attenuates doxorubicin resistance by reversing the epithelial-mesenchymal transition in breast cancer.

    abstract::Development of resistance to doxorubicin-based chemotherapy limits curative effect in breast cancer (BC). N-acetyltransferase 10 (NAT10), a nucleolar protein involved in histone acetylation, is overexpressed in several cancers. We investigated whether NAT10 is involved in doxorubicin resistance in BC and explored the ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wu J,Zhu H,Wu J,Chen W,Guan X

    更新日期:2018-01-15 00:00:00

  • Expression profile of ACTL8, CTCFL, OIP5 and XAGE3 in glioma and their prognostic significance: a retrospective clinical study.

    abstract:BACKGROUND:Cancer/testis antigens (CTAs) are attractive therapeutic targets for tumor immunotherapy due to their restrictive expression in normal testis but excessive in majority of tumor types. ACTL8, CTCFL, OIP5 and XAGE3 are members of the CTAs family. Currently, the data of ACTL8, CTCFL, OIP5 and XAGE3 expression i...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Li X,Ning L,Zhang Q,Ge Y,Liu C,Bi S,Zeng X,Nong W,Wu S,Guo G,Xiao S,Luo B,Xie X

    更新日期:2020-12-15 00:00:00

  • Comparison of MRI, CT and 18F-FDG PET/CT in the diagnosis of local and metastatic of nasopharyngeal carcinomas: an updated meta analysis of clinical studies.

    abstract:BACKGROUND:A meta-analysis was conducted to evaluate the accuracy of MRI, CT and FDG PET/CT in TNM stage of nasopharyngeal carcinoma patients (NPC). METHODS:Through a search of studies from 1996 to April 2015, pooled estimated sensitivity, specificity, pooled diagnostic odds ratio (DOR), summary receiver operating cha...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Chen WS,Li JJ,Hong L,Xing ZB,Wang F,Li CQ

    更新日期:2016-11-15 00:00:00

  • Normal peripheral prostate stromal cells stimulate prostate cancer development: roles of c-kit signal.

    abstract:BACKGROUND:To investigated the peripheral stromal cell conditioned medium (CM) -stimulated c-kit-JAK2-STAT1 pathway in prostate cancer. METHODS:CM harvested from normal prostate peripheral stromal cells was added to DU145 cells. DU145 cell viability and migration were measured by cell counting kit-8 reagent and Transw...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Guo JH,Zhou J,Zhao Y,Liu PY,Yao HJ,Da J,Zhang M,Zhou Z,Chen Q,Peng YB,Wang Z

    更新日期:2015-03-15 00:00:00

  • A medical mirroring: chronic myelomonocytic leukemia mimicking immunoglobulin G4-related disease.

    abstract::Chronic myelomonocytic leukemia (CMML) can be complicated by autoimmune features associated with rheumatologic disorders, which have been commonly reported by more researches currently. The intrinsic correlation between CMML and autoimmune diseases can create significant pitfalls in differential diagnosis. CMML occasi...

    journal_title:American journal of translational research

    pub_type:

    doi:

    authors: Bai Z,Chen Y,Chen X,Dong L

    更新日期:2019-07-15 00:00:00

  • Influence of acupuncture in treatment of knee osteoarthritis and cartilage repairing.

    abstract::As two major non-operative methods, physiotherapy and acupuncture have been proved to be safe and effective in osteoarthritis (OA) treatment. However, only a little study focused on functions of both methods on cartilage repairing. The main goal of this research is to prove and compare effectiveness of acupuncture and...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Zhang Y,Bao F,Wang Y,Wu Z

    更新日期:2016-09-15 00:00:00

  • A novel transverse talar tunnel achieved less vessel damage and better drilling safety for ATFL reconstruction: a cadaveric study with three-dimensional microCT.

    abstract:PURPOSE:To introduce a novel transverse tunnel (TT) in anterior talofibular ligament (ATFL) reconstruction, and assess whether it was superior to the tunnels currently used. METHODS:Thirteen fresh cadaveric lower extremities were perfused with lead-based contrast. Talar tunnels were drilled from the ATFL insertion in ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wang D,Shen Z,Yang S,Zhang B,Li Y,Fang Y,Jiao C,Guo Q,Zhang W,Jiang D

    更新日期:2020-12-15 00:00:00

  • Long noncoding RNA MEG3 suppressed endothelial cell proliferation and migration through regulating miR-21.

    abstract::Long non-coding RNAs (lncRNAs) act critical roles in many biological processes, including cell proliferation, apoptosis, development, invasion and migration. LncRNA maternally expressed gene 3 (MEG3) is found to be downregulated in several tumors; however, its role in the atherosclerosis is still unknown. In the prese...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wu Z,He Y,Li D,Fang X,Shang T,Zhang H,Zheng X

    更新日期:2017-07-15 00:00:00

  • Diosgenin increased DDX3 expression in hepatocellular carcinoma.

    abstract::Liver cancer, one of the most common malignant tumors occurred worldwide, has emerged as a main health trouble and accounts for leading cancer-related death. Diosgenin is provided as an important material in the pharmaceutical industry, and is used to manage various medical troubles such as cancer because of its multi...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Yu H,Liu Y,Niu C,Cheng Y

    更新日期:2018-11-15 00:00:00

  • Later phase cardioprotection of ischemic post-conditioning against ischemia/reperfusion injury depends on iNOS and PI3K-Akt pathway.

    abstract:BACKGROUND:The cardioprotection of ischemic post-conditioning (IPO) has been well demonstrated after a short period of reperfusion. However, little is known about the long-term effects of IPO. This study aimed to investigate the long term cardioprotection of IPO in a rat myocardial ischemia/reperfusion model and to exp...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wang G,Li X,Wang H,Wang Y,Zhang L,Zhang L,Liu B,Zhang M

    更新日期:2015-12-15 00:00:00

  • Profiling and functional characterization of circulation LncRNAs that are associated with coronary atherosclerotic plaque stability.

    abstract:BACKGROUND:Accumulating studies have demonstrated that some long non-coding RNAs (lncRNAs) play critical roles in the pathogenesis of atherosclerosis. We aimed to identify circulation lncRNAs that are potential biomarkers to evaluate coronary atherosclerotic plaque stability. METHODS AND RESULTS:The transcriptomes of ...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Pan Z,Fan Z,Ma J,Liu H,Shen L,He B,Zhang M

    更新日期:2019-06-15 00:00:00

  • The application of next-generation sequencing in diagnosing invasive pulmonary aspergillosis: three case reports.

    abstract::Invasive pulmonary aspergillosis (IPA) is a severe infectious disease with high mortality. However, the clinical diagnosis of IPA remains difficult since the microbiological evidence is hard to acquire. The main issue of the current microbiological methods is that they are time-consuming and offer a low yield. Current...

    journal_title:American journal of translational research

    pub_type:

    doi:

    authors: He BC,Liu LL,Chen BL,Zhang F,Su X

    更新日期:2019-04-15 00:00:00

  • VNN1 overexpression is associated with poor response to preoperative chemoradiotherapy and adverse prognosis in patients with rectal cancers.

    abstract:BACKGROUND:Colorectal cancer is prevalent worldwide and it is also the fourth most common cause of cancer mortality. For rectal cancer, neoadjuvant concurrent chemoradiotherapy (CCRT) followed by radical proctectomy is gold standard treatment for patients with stage II/III rectal cancer. By data mining a public dataset...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Chai CY,Zhang Y,Song J,Lin SC,Sun S,Chang IW

    更新日期:2016-10-15 00:00:00

  • Extinction bursts in rats trained to self-administer nicotine or food in 1-h daily sessions.

    abstract::Extinction bursts are characterized by a temporary increase in responding when drug access is withheld from rats trained to self-administer drugs of abuse. Thus far, one study has examined extinction bursts for nicotine self-administration using a 23-h access paradigm [1]. Here we examined extinction bursts using prev...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Pushparaj A,Pryslawsky Y,Forget B,Yan Y,Le Foll B

    更新日期:2012-01-01 00:00:00

  • Interleukin-21 is associated with the severity of psoriasis vulgaris through promoting CD4+ T cells to differentiate into Th17 cells.

    abstract::Interleukin-21 (IL-21) and T helper 17 (Th17) cells are known to be involved in the pathogenesis of psoriasis, but little is known about their relationship in psoriasis. Herein, we investigated whether IL-21 could regulate Th17 cell induction in patients with psoriasis vulgaris. 32 patients with psoriasis vulgaris and...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Wang Y,Wang LL,Yang HY,Wang FF,Zhang XX,Bai YP

    更新日期:2016-07-15 00:00:00

  • Downregulation of cathepsin G reduces the activation of CD4+ T cells in murine autoimmune diabetes.

    abstract::Type 1 diabetes mellitus (T1DM) is an autoimmune disease due to progressive injury of islet cells mediated by T lymphocytes (T cells). Our previous studies have shown that only cathepsin G (CatG), not other proteases, is involved in the antigen presentation of proinsulin, and if the presentation is inhibited, the acti...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Zou F,Lai X,Li J,Lei S,Hu L

    更新日期:2017-11-15 00:00:00

  • Galectin-1 mediates TGF-β-induced transformation from normal fibroblasts into carcinoma-associated fibroblasts and promotes tumor progression in gastric cancer.

    abstract::Rcinoma-associated fibroblasts (CAFs) are a major constituent of the tumor microenvironment. Cancer cells can induce the transformation from normal fibroblasts (NFs) into CAFs, reciprocally, CAFs promote tumor invasion and proliferation. TGF-β has been the mostly accepted factor to fuel NFs transformation into CAFs. G...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Zheng L,Xu C,Guan Z,Su X,Xu Z,Cao J,Teng L

    更新日期:2016-04-15 00:00:00

  • Over-expression of HSPA12B protects mice against myocardium ischemic/reperfusion injury through a PPARγ-dependent PI3K/Akt/eNOS pathway.

    abstract::Acute myocardial ischemia/reperfusion (MIR) injury leads to severe arrhythmias and a high lethality. We aim to determine the effect of heat shock protein A12B (HSPA12B), a newly discovered member of the Hsp70 family, on heart injury parameters following MIR surgery. We used HSPA12B transgenic mice to determine its eff...

    journal_title:American journal of translational research

    pub_type: 杂志文章,收录出版

    doi:

    authors: Sun Y,Ye L,Jiang C,Jiang J,Hong H,Qiu L

    更新日期:2015-12-15 00:00:00

  • Spermatogonial stem cells are a promising and pluripotent cell source for regenerative medicine.

    abstract::Regenerative medicine has been shown to hold enormous potential to treat traumatic and degenerative diseases, and substantial advancements have been made in the recent decades. In particular, different cell types were evaluated in basic research and preclinical studies on cell-based therapy applications. Despite the e...

    journal_title:American journal of translational research

    pub_type: 杂志文章,评审

    doi:

    authors: Chen Z,Hong F,Wang Z,Hao D,Yang H

    更新日期:2020-11-15 00:00:00

  • Effects of intermittent pressure imitating rolling manipulation in traditional Chinese medicine on ultrastructure and metabolism in injured human skeletal muscle cells.

    abstract::Skeletal muscle injuries can cause significant change in the ultrastructure and the metabolism of the skeletal muscle cells. Observation of the ultrastructure and measurements of the metabolism biomarkers such as total superoxide dismutase (T-SOD), malondialdehyde (MDA), and creatine kinase (CK) can be used to evaluat...

    journal_title:American journal of translational research

    pub_type: 杂志文章

    doi:

    authors: Guan H,Zhao L,Liu H,Xie D,Liu Y,Zhang G,Mason DC,Zhang S,Li Y,Zhang H

    更新日期:2020-01-15 00:00:00