Abstract:
:This study aimed to verify the effects of calpain on coxsackievirus B3 (CVB3)-induced myocarditis and to further explore the underlying mechanisms. Transgenic mice overexpressing calpastatin, the endogenous calpain inhibitor, were introduced in the present study. The murine model of viral myocarditis (VMC) was established by intraperitoneal injection of CVB3 into transgenic and wild-type mice. Myocardial injury was measured by H&E staining and ELISA for cTnI. CVB3 replication was assessed via capsid protein VP1 detection and virus titration. The fibrotic factors collagen and TGF-β1 were evaluated by Masson staining and real-time PCR analysis, respectively. Moreover, the levels of NLRP3, AIM2, ASC, cleaved caspase-1, cleaved caspase-11 and the pyroptosis indicators GSDMD p30, IL-1β and HMGB1 were determined by real-time PCR, western blot or immunohistochemical analysis. In addition, peripheral IL-1β and HMGB1 were evaluated by ELISA. We observed that CVB3-infected transgenic mice had lower pathological scores, peripheral cTnI levels, viral loads and expression levels of collagen and TGF-β1 in the heart than CVB3-infected wild-type mice. Furthermore, we found decreased levels of NLRP3, ASC, cleaved caspase-1 and cleaved caspase-11 in the hearts of CVB3-infected transgenic mice. However, after CVB3 infection, the levels of AIM2 in transgenic mice and wild-type mice did not differ significantly. Additionally, calpastatin overexpression significantly reduced the levels of GSDMD p30, IL-1β and HMGB1 in the myocardium as well as peripheral IL-1β and HMGB1. Taken together, these findings indicate that calpain inhibition attenuates CVB3-induced myocarditis by suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and noncanonical caspase-11-mediated pyroptosis pathways.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Yu Y,Shi H,Yu Y,Liu M,Li M,Liu X,Wang Y,Chen Rsubject
Has Abstractpub_date
2020-05-15 00:00:00pages
1954-1964issue
5issn
1943-8141journal_volume
12pub_type
杂志文章abstract::Increasing evidence demonstrate that dysregulated microRNAs (miRNAs) are involved in carcinogenesis and tumor progression in papillary thyroid cancer (PTC). However, the specific miR-761 in cancer remains largely unknown. In this study, we reported for the first that miR-761 expression was down-regulated in PTC tissue...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-11-15 00:00:00
abstract::The microRNA-29 family, which contains mir-29a, mir-29b, and mir-29c, can promote or resist the development of several types of tumors. However, its role in rhabdomyosarcoma (RMS) has not been determined. In this work, we detected the expression of mir-29a/b/c in RMS. Results showed that the tissues and cell lines in ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-03-15 00:00:00
abstract::Depression is prevalence in patients with laryngeal cancer. In this study, we aim to investigate whether psychological intervention could reduce the depression of patients receiving cancer treatment. In this study, 258 patients with laryngeal cancer were assigned into two groups with or without psychological intervent...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-10-15 00:00:00
abstract::The house dust mite (HDM), Dermatophagoidesfarinae (D. farina), is one of the most important indoor allergen sources and a major elicitor of allergic asthma; itscharacterization is important in the diagnosis and immunotherapy of mite allergen-relevant diseases. This study aims to characterize a novel allergen, the D. ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-07-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-08-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-09-15 00:00:00
abstract::Background: Mitochondrial flashes (mitoflashes) are transient signals from transient bursts of reactive oxygen species (ROS) and changes in pH that occur in certain physiological or pathological conditions. Mitoflashes are closely related to metabolism, cell differentiation, stress response, diseases, and aging. Sepsi...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2021-01-15 00:00:00
abstract::Neural Wiskott-Aldrich syndrome protein (N-WASP) is an important member of the WASP family involved in the actin cytoskeleton reorganization. Recent evidence suggests that N-WASP may play important roles in tumor progression and metastasis. However, the contribution of N-WASP to cervical cancer is still unknown. The p...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-02-15 00:00:00
abstract:PURPOSE:This study aimed to determine whether chronic intraocular pressure (IOP) elevation induces retinal oxidative stress and alters mitochondrial morphology and function of retinal ganglion cells (RGC) and to explore the effects of AAV-SOD2 gene therapy on the RGC survival and mitochondrial dysfunction. METHODS:Chr...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-02-15 00:00:00
abstract:BACKGROUND:Circular RNAs (circRNAs) represent a class of non-coding RNAs that are emerging as important regulators during tumorigenesis and provide potential targets for cancer intervention. However, the expression profiles and functions of circRNAs in hepatocellular carcinoma (HCC) have not been completely clarified. ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-06-15 00:00:00
abstract::Skeletal muscle injuries can cause significant change in the ultrastructure and the metabolism of the skeletal muscle cells. Observation of the ultrastructure and measurements of the metabolism biomarkers such as total superoxide dismutase (T-SOD), malondialdehyde (MDA), and creatine kinase (CK) can be used to evaluat...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-01-15 00:00:00
abstract::As two major non-operative methods, physiotherapy and acupuncture have been proved to be safe and effective in osteoarthritis (OA) treatment. However, only a little study focused on functions of both methods on cartilage repairing. The main goal of this research is to prove and compare effectiveness of acupuncture and...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-09-15 00:00:00
abstract::NSUN5, a gene encodes a cytosine-5 RNA methyltransferase, is rarely mentioned in cancers. Our study is the first one to evaluate the role of NSUN5 in the progression of colorectal cancer. Data from TCGA was used to show the different expression of NSUN5 between CRC tumor tissues and adjacent normal ones. The NSUN5 exp...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-07-15 00:00:00
abstract::Circular RNA hsa_circ_0002024 has been reported to be underexpressed in bladder cancer (BC). However, the biological role of hsa_circ_0002024 in BC and its underlying molecular mechanisms remain unclear. In this study, the expression levels of hsa_circ_0002024 and miR-197-3p were examined by quantitative real-time pol...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-03-15 00:00:00
abstract::LncRNAs have been shown to invovled in the development of various cancers. However, the function and potential mechanism of PTCSC3 in cervical carcinoma progression remains unknown. In this reference, we showed that PTCSC3 expression was downregulated in 4 cervical carcinoma cell lines (Caski, Siha, Hela and C4-1) com...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-11-15 00:00:00
abstract::RAB18, a member of the Ras family, has been suggested to play a critical role in multiple biological process. However, its functions in the development of hepatocellular carcinoma (HCC) remain unknown. In the present study, the expression and biological role of RAB18 in HCC were investigated. Results showed that the e...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-02-15 00:00:00
abstract::Alopecia areata is characterized by the loss of hair on the scalp and elsewhere on the body. It affects approximately 2% of the general population. It is believed to be an autoimmune disease. However, its pathogenesis remains incompletely understood. Recent studies have revealed a substantial link between vitamin D an...
journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2019-09-15 00:00:00
abstract::Bronchopulmonary dysplasia (BPD) is one of the most common causes of mortality and morbidity in neonatal intensive care units. Persistent inflammation, with an abnormal influx of polymorphonuclear leukocytes (PMNs) followed by monocytes (MONOs), occurs early in the pathogenesis of BPD. Anti-inflammatory therapy with b...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Objectives: Persistent low-grade chronic inflammation is common in type 2 diabetes (T2D) and obesity. To date, the underlying molecular mechanism is not well understood. In this study, we aimed to investigate gut microbiota and the expression of monocyte surface molecules in obese T2D subjects who underwent Roux-en-Y ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-03-15 00:00:00
abstract:BACKGROUND:Systemic maternal inflammation and neonatal hyperoxia arrest alveolarization in neonates. The aims were to test whether human mesenchymal stem cells (MSCs) reduce lung inflammation and improve lung development in perinatal inflammation- and hyperoxia-induced experimental bronchopulmonary dysplasia. METHODS:...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-02-15 00:00:00
abstract::The abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are crucial pathological processes that are involved in atherosclerosis. Growing evidence suggests that microRNAs (miRNAs) play critical roles in VSMCs functions. Here, we analyzed the expression of four atherosclerosis-related miRNAs and...
journal_title:American journal of translational research
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doi:
更新日期:2017-02-15 00:00:00
abstract:OBJECTIVE:To explore the utility of dual-energy spectral computed tomography (CT) in the differential diagnosis of focal organizing pneumonia (FOP) and solitary bronchioloalveolar carcinoma (S-BAC). MATERIALS AND METHODS:The institutional review board approved this study and waived the requirement for informed consent...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-07-15 00:00:00
abstract::Prostate cancer (PCa) is a leading cause of cancer-related deaths among men. The anthracycline doxorubicin (DOX) is used for the treatment of this disease, but its considerable side effects and non-selectivity are major drawbacks. Simvastatin (Sim), a lipid-lowering agent, holds great promise as a cancer therapeutic, ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-03-15 00:00:00
abstract::Acute myocardial ischemia/reperfusion (MIR) injury leads to severe arrhythmias and a high lethality. We aim to determine the effect of heat shock protein A12B (HSPA12B), a newly discovered member of the Hsp70 family, on heart injury parameters following MIR surgery. We used HSPA12B transgenic mice to determine its eff...
journal_title:American journal of translational research
pub_type: 杂志文章,收录出版
doi:
更新日期:2015-12-15 00:00:00
abstract:BACKGROUND:A meta-analysis was conducted to evaluate the accuracy of MRI, CT and FDG PET/CT in TNM stage of nasopharyngeal carcinoma patients (NPC). METHODS:Through a search of studies from 1996 to April 2015, pooled estimated sensitivity, specificity, pooled diagnostic odds ratio (DOR), summary receiver operating cha...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-11-15 00:00:00
abstract::MicroRNAs (miRNAs) are small, non-coding RNAs which can function as oncogenes or tumor suppressor genes in human cancers. In the present study, we demonstrated that the expression ofmiR-133a was dramatically decreased in examined esophageal squamous cell carcinoma (ESCC) cell lines and clinical ESCC tissue samples. Ad...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-08-15 00:00:00
abstract::The effect of different static magnetic field strengths, 1 T or 7 T, on the quality of nitroxyl radical-based magnetic resonance redox imaging (MRRI) was examined. A stable nitroxyl radical, 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (MC-PROXYL), was used as a T1 contrast agent. Phantoms and animals were ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-10-15 00:00:00
abstract::Colorectal cancer (CRC) is one of the most commonly diagnosed cancers, and it has a poor prognosis. Emerging evidence shows that circular RNAs (circRNAs) may act as good therapeutic targets for cancers due to their abundance and stability. However, their regulatory role in CRC needs further investigation. This study r...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-06-15 00:00:00